CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/289.mrc Output diagnostic file name Micrographs_0601/289.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/289.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2508.000 8880.000 Mean, RMS : 4437.267 210.6614 TITLE 1: emi2mrc: 289.emi Sun Jan 05 22:04:00 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.08%, Obj 88.09%, Dif 73.67% TITLE 3: X 39.359, Y 596.488, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01758 10200.00 10000.00 0.00 0.01900 10400.00 10000.00 0.00 0.02047 10600.00 10000.00 0.00 0.02193 10800.00 10000.00 0.00 0.02333 11000.00 10000.00 0.00 0.02504 11200.00 10000.00 0.00 0.02669 11400.00 10000.00 0.00 0.02821 11600.00 10000.00 0.00 0.02987 11800.00 10000.00 0.00 0.03157 12000.00 10000.00 0.00 0.03359 12200.00 10000.00 0.00 0.03580 12400.00 10000.00 0.00 0.03813 12600.00 10000.00 0.00 0.04035 12800.00 10000.00 0.00 0.04261 13000.00 10000.00 0.00 0.04504 13200.00 10000.00 0.00 0.04728 13400.00 10000.00 0.00 0.04948 13600.00 10000.00 0.00 0.05173 13800.00 10000.00 0.00 0.05416 14000.00 10000.00 0.00 0.05672 14200.00 10000.00 0.00 0.05924 14400.00 10000.00 0.00 0.06186 14600.00 10000.00 0.00 0.06456 14800.00 10000.00 0.00 0.06706 15000.00 10000.00 0.00 0.06952 15200.00 10000.00 0.00 0.07198 15400.00 10000.00 0.00 0.07397 15600.00 10000.00 0.00 0.07541 15800.00 10000.00 0.00 0.07638 16000.00 10000.00 0.00 0.07723 16200.00 10000.00 0.00 0.07765 15800.00 10200.00 0.00 0.07805 16000.00 10200.00 0.00 0.07887 16200.00 10200.00 0.00 0.07925 15800.00 10400.00 0.00 0.07972 16000.00 10400.00 0.00 0.08052 16200.00 10400.00 0.00 0.08085 15800.00 10600.00 0.00 0.08143 16000.00 10600.00 0.00 0.08217 16200.00 10600.00 0.00 0.08240 15800.00 10800.00 0.00 0.08300 16000.00 10800.00 0.00 0.08366 16200.00 10800.00 0.00 0.08381 15600.00 11000.00 0.00 0.08384 15800.00 11000.00 0.00 0.08471 16000.00 11000.00 0.00 0.08533 16200.00 11000.00 0.00 0.08545 15600.00 11200.00 0.00 0.08566 15800.00 11200.00 0.00 0.08654 16000.00 11200.00 0.00 0.08712 16200.00 11200.00 0.00 0.08721 15600.00 11400.00 0.00 0.08786 15800.00 11400.00 0.00 0.08873 16000.00 11400.00 0.00 0.08923 16200.00 11400.00 0.00 0.08927 15600.00 11600.00 0.00 0.09013 15800.00 11600.00 0.00 0.09089 16000.00 11600.00 0.00 0.09128 16200.00 11600.00 0.00 0.09129 15600.00 11800.00 0.00 0.09208 15800.00 11800.00 0.00 0.09274 16000.00 11800.00 0.00 0.09308 16200.00 11800.00 0.00 0.09309 15600.00 12000.00 0.00 0.09400 15800.00 12000.00 0.00 0.09462 16000.00 12000.00 0.00 0.09495 15600.00 12200.00 0.00 0.09611 15800.00 12200.00 0.00 0.09666 16000.00 12200.00 0.00 0.09694 15600.00 12400.00 0.00 0.09807 15800.00 12400.00 0.00 0.09855 16000.00 12400.00 0.00 0.09877 15400.00 12600.00 0.00 0.09878 15600.00 12600.00 0.00 0.10016 15800.00 12600.00 0.00 0.10063 16000.00 12600.00 0.00 0.10085 15400.00 12800.00 0.00 0.10140 15600.00 12800.00 0.00 0.10276 15800.00 12800.00 0.00 0.10318 16000.00 12800.00 0.00 0.10328 15400.00 13000.00 0.00 0.10386 15600.00 13000.00 0.00 0.10512 15800.00 13000.00 0.00 0.10541 15400.00 13200.00 0.00 0.10637 15600.00 13200.00 0.00 0.10748 15800.00 13200.00 0.00 0.10761 15400.00 13400.00 0.00 0.10855 15600.00 13400.00 0.00 0.10955 15800.00 13400.00 0.00 0.10955 15400.00 13600.00 0.00 0.11085 15600.00 13600.00 0.00 0.11177 15400.00 13800.00 0.00 0.11341 15600.00 13800.00 0.00 0.11424 15400.00 14000.00 0.00 0.11573 15600.00 14000.00 0.00 0.11638 15400.00 14200.00 0.00 0.11817 15600.00 14200.00 0.00 0.11858 15400.00 14400.00 0.00 0.12005 15600.00 14400.00 0.00 0.12024 15200.00 14600.00 0.00 0.12063 15400.00 14600.00 0.00 0.12204 15200.00 14800.00 0.00 0.12273 15400.00 14800.00 0.00 0.12394 15200.00 15000.00 0.00 0.12494 15400.00 15000.00 0.00 0.12574 15200.00 15200.00 0.00 0.12689 15400.00 15200.00 0.00 0.12699 15200.00 15400.00 0.00 0.12739 15200.00 15600.00 0.00 0.12752 15200.00 15600.00 5.00 0.12772 15200.00 15600.00 10.00 0.12787 15200.00 15600.00 15.00 0.12798 15200.00 15600.00 20.00 0.12804 15200.00 15600.00 25.00 0.12807 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15235.00 15600.00 25.00 0.12811 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/289.ctf NX, NY, NZ: 512 512 1 MODE : real