CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/286.mrc Output diagnostic file name Micrographs_0601/286.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/286.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2388.000 9004.000 Mean, RMS : 4439.395 218.2086 TITLE 1: emi2mrc: 286.emi Sun Jan 05 22:01:26 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.08%, Obj 88.09%, Dif 73.67% TITLE 3: X 44.527, Y 599.239, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02150 10200.00 10000.00 0.00 0.02239 10400.00 10000.00 0.00 0.02307 10600.00 10000.00 0.00 0.02384 10800.00 10000.00 0.00 0.02471 11000.00 10000.00 0.00 0.02577 11200.00 10000.00 0.00 0.02687 11400.00 10000.00 0.00 0.02791 11600.00 10000.00 0.00 0.02898 11800.00 10000.00 0.00 0.03018 12000.00 10000.00 0.00 0.03155 12200.00 10000.00 0.00 0.03297 12400.00 10000.00 0.00 0.03449 12600.00 10000.00 0.00 0.03613 12800.00 10000.00 0.00 0.03800 13000.00 10000.00 0.00 0.03975 13200.00 10000.00 0.00 0.04143 13400.00 10000.00 0.00 0.04330 13600.00 10000.00 0.00 0.04518 13800.00 10000.00 0.00 0.04729 14000.00 10000.00 0.00 0.04951 14200.00 10000.00 0.00 0.05171 14400.00 10000.00 0.00 0.05398 14600.00 10000.00 0.00 0.05638 14800.00 10000.00 0.00 0.05883 15000.00 10000.00 0.00 0.06140 15200.00 10000.00 0.00 0.06408 15400.00 10000.00 0.00 0.06671 15600.00 10000.00 0.00 0.06958 15800.00 10000.00 0.00 0.07249 16000.00 10000.00 0.00 0.07550 16200.00 10000.00 0.00 0.07860 16400.00 10000.00 0.00 0.08136 16600.00 10000.00 0.00 0.08319 16800.00 10000.00 0.00 0.08385 17000.00 10000.00 0.00 0.08413 17200.00 10000.00 0.00 0.08414 16600.00 10200.00 0.00 0.08446 16800.00 10200.00 0.00 0.08510 17000.00 10200.00 0.00 0.08535 16600.00 10400.00 0.00 0.08581 16800.00 10400.00 0.00 0.08643 17000.00 10400.00 0.00 0.08666 16600.00 10600.00 0.00 0.08726 16800.00 10600.00 0.00 0.08788 17000.00 10600.00 0.00 0.08812 16600.00 10800.00 0.00 0.08880 16800.00 10800.00 0.00 0.08941 17000.00 10800.00 0.00 0.08966 16600.00 11000.00 0.00 0.09053 16800.00 11000.00 0.00 0.09113 17000.00 11000.00 0.00 0.09137 16600.00 11200.00 0.00 0.09225 16800.00 11200.00 0.00 0.09286 17000.00 11200.00 0.00 0.09305 16600.00 11400.00 0.00 0.09417 16800.00 11400.00 0.00 0.09471 17000.00 11400.00 0.00 0.09483 16600.00 11600.00 0.00 0.09587 16800.00 11600.00 0.00 0.09635 17000.00 11600.00 0.00 0.09644 16600.00 11800.00 0.00 0.09764 16800.00 11800.00 0.00 0.09814 17000.00 11800.00 0.00 0.09827 16600.00 12000.00 0.00 0.09972 16800.00 12000.00 0.00 0.10027 17000.00 12000.00 0.00 0.10041 16600.00 12200.00 0.00 0.10206 16800.00 12200.00 0.00 0.10262 17000.00 12200.00 0.00 0.10271 16600.00 12400.00 0.00 0.10450 16800.00 12400.00 0.00 0.10503 16600.00 12600.00 0.00 0.10684 16800.00 12600.00 0.00 0.10727 16400.00 12800.00 0.00 0.10747 16600.00 12800.00 0.00 0.10937 16800.00 12800.00 0.00 0.10970 16400.00 13000.00 0.00 0.10985 16600.00 13000.00 0.00 0.11174 16800.00 13000.00 0.00 0.11198 16400.00 13200.00 0.00 0.11237 16600.00 13200.00 0.00 0.11427 16800.00 13200.00 0.00 0.11446 16400.00 13400.00 0.00 0.11499 16600.00 13400.00 0.00 0.11691 16800.00 13400.00 0.00 0.11702 16400.00 13600.00 0.00 0.11773 16600.00 13600.00 0.00 0.11961 16400.00 13800.00 0.00 0.12055 16600.00 13800.00 0.00 0.12237 16400.00 14000.00 0.00 0.12320 16600.00 14000.00 0.00 0.12496 16400.00 14200.00 0.00 0.12599 16600.00 14200.00 0.00 0.12766 16400.00 14400.00 0.00 0.12872 16600.00 14400.00 0.00 0.13027 16400.00 14600.00 0.00 0.13178 16600.00 14600.00 0.00 0.13318 16400.00 14800.00 0.00 0.13466 16600.00 14800.00 0.00 0.13587 16400.00 15000.00 0.00 0.13754 16600.00 15000.00 0.00 0.13854 16400.00 15200.00 0.00 0.14054 16600.00 15200.00 0.00 0.14131 16400.00 15400.00 0.00 0.14351 16600.00 15400.00 0.00 0.14406 16400.00 15600.00 0.00 0.14666 16600.00 15600.00 0.00 0.14692 16200.00 15800.00 0.00 0.14695 16400.00 15800.00 0.00 0.14942 16200.00 16000.00 0.00 0.15024 16400.00 16000.00 0.00 0.15231 16200.00 16200.00 0.00 0.15281 16400.00 16200.00 0.00 0.15445 16200.00 16400.00 0.00 0.15448 16400.00 16400.00 0.00 0.15570 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 16337.11 16485.19 34.95 0.15598 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/286.ctf NX, NY, NZ: 512 512 1 MODE : real