CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/285.mrc Output diagnostic file name Micrographs_0601/285.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/285.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2436.000 8962.000 Mean, RMS : 4479.947 218.9155 TITLE 1: emi2mrc: 285.emi Sun Jan 05 21:59:17 2014 TITLE 2: kV 300, Mag 78000x, Def 24280, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 50.126, Y 598.643, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01210 14200.00 10000.00 0.00 -0.01205 14400.00 10000.00 0.00 -0.01192 14600.00 10000.00 0.00 -0.01163 14800.00 10000.00 0.00 -0.01113 15000.00 10000.00 0.00 -0.01057 15200.00 10000.00 0.00 -0.01014 15400.00 10000.00 0.00 -0.00978 15600.00 10000.00 0.00 -0.00934 15800.00 10000.00 0.00 -0.00902 16000.00 10000.00 0.00 -0.00888 16200.00 10000.00 0.00 -0.00865 16400.00 10000.00 0.00 -0.00826 16600.00 10000.00 0.00 -0.00780 16800.00 10000.00 0.00 -0.00734 17000.00 10000.00 0.00 -0.00674 17200.00 10000.00 0.00 -0.00604 17400.00 10000.00 0.00 -0.00545 17600.00 10000.00 0.00 -0.00476 17800.00 10000.00 0.00 -0.00400 18000.00 10000.00 0.00 -0.00328 18200.00 10000.00 0.00 -0.00242 18400.00 10000.00 0.00 -0.00148 18600.00 10000.00 0.00 -0.00069 18800.00 10000.00 0.00 -0.00006 19000.00 10000.00 0.00 0.00056 19200.00 10000.00 0.00 0.00124 19400.00 10000.00 0.00 0.00199 19600.00 10000.00 0.00 0.00271 19800.00 10000.00 0.00 0.00343 20000.00 10000.00 0.00 0.00430 20200.00 10000.00 0.00 0.00514 20400.00 10000.00 0.00 0.00583 20600.00 10000.00 0.00 0.00661 20800.00 10000.00 0.00 0.00757 21000.00 10000.00 0.00 0.00847 21200.00 10000.00 0.00 0.00923 21400.00 10000.00 0.00 0.00998 21600.00 10000.00 0.00 0.01087 21800.00 10000.00 0.00 0.01192 22000.00 10000.00 0.00 0.01290 22200.00 10000.00 0.00 0.01389 22400.00 10000.00 0.00 0.01514 22600.00 10000.00 0.00 0.01653 22800.00 10000.00 0.00 0.01785 23000.00 10000.00 0.00 0.01909 23200.00 10000.00 0.00 0.02036 23400.00 10000.00 0.00 0.02172 23600.00 10000.00 0.00 0.02314 23800.00 10000.00 0.00 0.02458 24000.00 10000.00 0.00 0.02607 24200.00 10000.00 0.00 0.02759 24400.00 10000.00 0.00 0.02917 24600.00 10000.00 0.00 0.03099 24800.00 10000.00 0.00 0.03291 25000.00 10000.00 0.00 0.03468 25200.00 10000.00 0.00 0.03625 25400.00 10000.00 0.00 0.03724 25600.00 10000.00 0.00 0.03779 25800.00 10000.00 0.00 0.03817 25800.00 10200.00 0.00 0.03851 26000.00 10200.00 0.00 0.03854 25800.00 10400.00 0.00 0.03877 26000.00 10400.00 0.00 0.03879 25800.00 10600.00 0.00 0.03903 26000.00 10600.00 0.00 0.03906 25800.00 10800.00 0.00 0.03941 26000.00 10800.00 0.00 0.03946 25800.00 11000.00 0.00 0.03971 26000.00 11000.00 0.00 0.03975 25800.00 11200.00 0.00 0.03996 26000.00 11200.00 0.00 0.03998 25600.00 11400.00 0.00 0.04003 25800.00 11400.00 0.00 0.04036 26000.00 11400.00 0.00 0.04038 25600.00 11600.00 0.00 0.04042 25800.00 11600.00 0.00 0.04074 25600.00 11800.00 0.00 0.04096 25800.00 11800.00 0.00 0.04122 25600.00 12000.00 0.00 0.04139 25800.00 12000.00 0.00 0.04162 25600.00 12200.00 0.00 0.04180 25800.00 12200.00 0.00 0.04208 25600.00 12400.00 0.00 0.04244 25800.00 12400.00 0.00 0.04276 26000.00 12400.00 0.00 0.04279 25600.00 12600.00 0.00 0.04305 25800.00 12600.00 0.00 0.04335 25600.00 12800.00 0.00 0.04360 25800.00 12800.00 0.00 0.04388 25600.00 13000.00 0.00 0.04404 25800.00 13000.00 0.00 0.04433 26000.00 13000.00 0.00 0.04435 25600.00 13200.00 0.00 0.04461 25800.00 13200.00 0.00 0.04491 26000.00 13200.00 0.00 0.04492 25600.00 13400.00 0.00 0.04521 25800.00 13400.00 0.00 0.04549 25600.00 13600.00 0.00 0.04581 25800.00 13600.00 0.00 0.04608 25600.00 13800.00 0.00 0.04653 25800.00 13800.00 0.00 0.04680 25600.00 14000.00 0.00 0.04721 25800.00 14000.00 0.00 0.04751 25600.00 14200.00 0.00 0.04793 25800.00 14200.00 0.00 0.04826 26000.00 14200.00 0.00 0.04826 25600.00 14400.00 0.00 0.04860 25800.00 14400.00 0.00 0.04891 25600.00 14600.00 0.00 0.04928 25800.00 14600.00 0.00 0.04952 25600.00 14800.00 0.00 0.04991 25800.00 14800.00 0.00 0.05012 25600.00 15000.00 0.00 0.05065 25800.00 15000.00 0.00 0.05081 25400.00 15200.00 0.00 0.05086 25600.00 15200.00 0.00 0.05140 25800.00 15200.00 0.00 0.05150 25400.00 15400.00 0.00 0.05152 25600.00 15400.00 0.00 0.05199 25800.00 15400.00 0.00 0.05202 25400.00 15600.00 0.00 0.05219 25600.00 15600.00 0.00 0.05257 25400.00 15800.00 0.00 0.05282 25600.00 15800.00 0.00 0.05312 25400.00 16000.00 0.00 0.05353 25600.00 16000.00 0.00 0.05382 25800.00 16000.00 0.00 0.05383 25400.00 16200.00 0.00 0.05428 25600.00 16200.00 0.00 0.05458 25800.00 16200.00 0.00 0.05463 25400.00 16400.00 0.00 0.05514 25600.00 16400.00 0.00 0.05546 25800.00 16400.00 0.00 0.05556 25400.00 16600.00 0.00 0.05614 25600.00 16600.00 0.00 0.05649 25800.00 16600.00 0.00 0.05663 25400.00 16800.00 0.00 0.05714 25600.00 16800.00 0.00 0.05752 25800.00 16800.00 0.00 0.05765 25400.00 17000.00 0.00 0.05816 25600.00 17000.00 0.00 0.05852 25800.00 17000.00 0.00 0.05862 25400.00 17200.00 0.00 0.05896 25600.00 17200.00 0.00 0.05929 25800.00 17200.00 0.00 0.05936 25400.00 17400.00 0.00 0.05977 25600.00 17400.00 0.00 0.06012 25800.00 17400.00 0.00 0.06021 25400.00 17600.00 0.00 0.06082 25600.00 17600.00 0.00 0.06119 25800.00 17600.00 0.00 0.06131 25400.00 17800.00 0.00 0.06185 25600.00 17800.00 0.00 0.06223 25800.00 17800.00 0.00 0.06236 25400.00 18000.00 0.00 0.06297 25600.00 18000.00 0.00 0.06335 25800.00 18000.00 0.00 0.06348 25400.00 18200.00 0.00 0.06420 25600.00 18200.00 0.00 0.06458 25800.00 18200.00 0.00 0.06469 25400.00 18400.00 0.00 0.06528 25600.00 18400.00 0.00 0.06568 25800.00 18400.00 0.00 0.06578 25400.00 18600.00 0.00 0.06646 25600.00 18600.00 0.00 0.06687 25800.00 18600.00 0.00 0.06695 25400.00 18800.00 0.00 0.06775 25600.00 18800.00 0.00 0.06814 25800.00 18800.00 0.00 0.06817 25400.00 19000.00 0.00 0.06902 25600.00 19000.00 0.00 0.06939 25400.00 19200.00 0.00 0.07039 25600.00 19200.00 0.00 0.07074 25400.00 19400.00 0.00 0.07166 25600.00 19400.00 0.00 0.07196 25200.00 19600.00 0.00 0.07198 25400.00 19600.00 0.00 0.07294 25600.00 19600.00 0.00 0.07317 25200.00 19800.00 0.00 0.07322 25400.00 19800.00 0.00 0.07412 25600.00 19800.00 0.00 0.07429 25400.00 20000.00 0.00 0.07509 25600.00 20000.00 0.00 0.07523 25200.00 20200.00 0.00 0.07523 25400.00 20200.00 0.00 0.07611 25600.00 20200.00 0.00 0.07623 25200.00 20400.00 0.00 0.07633 25400.00 20400.00 0.00 0.07721 25600.00 20400.00 0.00 0.07730 25200.00 20600.00 0.00 0.07763 25400.00 20600.00 0.00 0.07848 25600.00 20600.00 0.00 0.07852 25200.00 20800.00 0.00 0.07912 25400.00 20800.00 0.00 0.07992 25200.00 21000.00 0.00 0.08059 25400.00 21000.00 0.00 0.08132 25200.00 21200.00 0.00 0.08189 25400.00 21200.00 0.00 0.08259 25200.00 21400.00 0.00 0.08332 25400.00 21400.00 0.00 0.08401 25200.00 21600.00 0.00 0.08474 25400.00 21600.00 0.00 0.08546 25200.00 21800.00 0.00 0.08619 25400.00 21800.00 0.00 0.08691 25200.00 22000.00 0.00 0.08775 25400.00 22000.00 0.00 0.08843 25200.00 22200.00 0.00 0.08924 25400.00 22200.00 0.00 0.08985 25200.00 22400.00 0.00 0.09101 25400.00 22400.00 0.00 0.09159 25200.00 22600.00 0.00 0.09287 25400.00 22600.00 0.00 0.09338 25200.00 22800.00 0.00 0.09471 25400.00 22800.00 0.00 0.09512 25200.00 23000.00 0.00 0.09648 25400.00 23000.00 0.00 0.09679 25200.00 23200.00 0.00 0.09803 25400.00 23200.00 0.00 0.09825 25200.00 23400.00 0.00 0.09956 25400.00 23400.00 0.00 0.09967 25200.00 23600.00 0.00 0.10119 25000.00 23800.00 0.00 0.10133 25200.00 23800.00 0.00 0.10285 25000.00 24000.00 0.00 0.10290 25200.00 24000.00 0.00 0.10434 25000.00 24200.00 0.00 0.10454 25200.00 24200.00 0.00 0.10587 25000.00 24400.00 0.00 0.10591 25200.00 24400.00 0.00 0.10715 25000.00 24600.00 0.00 0.10740 25200.00 24600.00 0.00 0.10851 25000.00 24800.00 0.00 0.10897 25200.00 24800.00 0.00 0.10990 25000.00 25000.00 0.00 0.11024 25200.00 25000.00 0.00 0.11096 25000.00 25200.00 0.00 0.11130 25200.00 25200.00 0.00 0.11154 25000.00 25200.00 15.00 0.11163 25000.00 25200.00 20.00 0.11171 25000.00 25200.00 25.00 0.11177 25000.00 25200.00 30.00 0.11182 25000.00 25200.00 35.00 0.11183 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25051.07 25253.44 27.84 0.11201 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/285.ctf NX, NY, NZ: 512 512 1 MODE : real