CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/284.mrc Output diagnostic file name Micrographs_0601/284.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/284.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2342.000 8846.000 Mean, RMS : 4478.719 218.4569 TITLE 1: emi2mrc: 284.emi Sun Jan 05 21:57:51 2014 TITLE 2: kV 300, Mag 78000x, Def 24280, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 55.294, Y 597.743, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01046 10200.00 10000.00 0.00 -0.01039 10400.00 10000.00 0.00 -0.01028 11400.00 10000.00 0.00 -0.01020 11600.00 10000.00 0.00 -0.01005 11800.00 10000.00 0.00 -0.00998 12000.00 10000.00 0.00 -0.00996 14400.00 10000.00 0.00 -0.00969 14600.00 10000.00 0.00 -0.00951 14800.00 10000.00 0.00 -0.00930 15000.00 10000.00 0.00 -0.00903 15200.00 10000.00 0.00 -0.00884 15400.00 10000.00 0.00 -0.00858 15600.00 10000.00 0.00 -0.00826 15800.00 10000.00 0.00 -0.00772 16000.00 10000.00 0.00 -0.00713 16200.00 10000.00 0.00 -0.00655 16400.00 10000.00 0.00 -0.00580 16600.00 10000.00 0.00 -0.00505 16800.00 10000.00 0.00 -0.00428 17000.00 10000.00 0.00 -0.00354 17200.00 10000.00 0.00 -0.00278 17400.00 10000.00 0.00 -0.00200 17600.00 10000.00 0.00 -0.00120 17800.00 10000.00 0.00 -0.00029 18000.00 10000.00 0.00 0.00053 18200.00 10000.00 0.00 0.00133 18400.00 10000.00 0.00 0.00222 18600.00 10000.00 0.00 0.00315 18800.00 10000.00 0.00 0.00425 19000.00 10000.00 0.00 0.00525 19200.00 10000.00 0.00 0.00608 19400.00 10000.00 0.00 0.00695 19600.00 10000.00 0.00 0.00791 19800.00 10000.00 0.00 0.00896 20000.00 10000.00 0.00 0.00999 20200.00 10000.00 0.00 0.01100 20400.00 10000.00 0.00 0.01194 20600.00 10000.00 0.00 0.01280 20800.00 10000.00 0.00 0.01375 21000.00 10000.00 0.00 0.01474 21200.00 10000.00 0.00 0.01569 21400.00 10000.00 0.00 0.01674 21600.00 10000.00 0.00 0.01804 21800.00 10000.00 0.00 0.01937 22000.00 10000.00 0.00 0.02055 22200.00 10000.00 0.00 0.02175 22400.00 10000.00 0.00 0.02310 22600.00 10000.00 0.00 0.02449 22800.00 10000.00 0.00 0.02584 23000.00 10000.00 0.00 0.02716 23200.00 10000.00 0.00 0.02864 23400.00 10000.00 0.00 0.03033 23600.00 10000.00 0.00 0.03198 23800.00 10000.00 0.00 0.03378 24000.00 10000.00 0.00 0.03567 24200.00 10000.00 0.00 0.03757 24400.00 10000.00 0.00 0.03939 24600.00 10000.00 0.00 0.04064 24800.00 10000.00 0.00 0.04122 25000.00 10000.00 0.00 0.04134 25200.00 10000.00 0.00 0.04137 24800.00 10200.00 0.00 0.04162 25000.00 10200.00 0.00 0.04179 25200.00 10200.00 0.00 0.04183 24800.00 10400.00 0.00 0.04216 25000.00 10400.00 0.00 0.04236 25200.00 10400.00 0.00 0.04241 24800.00 10600.00 0.00 0.04250 25000.00 10600.00 0.00 0.04273 25200.00 10600.00 0.00 0.04280 24800.00 10800.00 0.00 0.04298 25000.00 10800.00 0.00 0.04325 25200.00 10800.00 0.00 0.04334 24800.00 11000.00 0.00 0.04364 25000.00 11000.00 0.00 0.04390 25200.00 11000.00 0.00 0.04396 24800.00 11200.00 0.00 0.04403 25000.00 11200.00 0.00 0.04424 25200.00 11200.00 0.00 0.04427 24800.00 11400.00 0.00 0.04443 25000.00 11400.00 0.00 0.04461 25200.00 11400.00 0.00 0.04463 24800.00 11600.00 0.00 0.04482 25000.00 11600.00 0.00 0.04501 25200.00 11600.00 0.00 0.04506 24800.00 11800.00 0.00 0.04524 25000.00 11800.00 0.00 0.04549 25200.00 11800.00 0.00 0.04553 24800.00 12000.00 0.00 0.04573 25000.00 12000.00 0.00 0.04598 24800.00 12200.00 0.00 0.04622 25000.00 12200.00 0.00 0.04643 24800.00 12400.00 0.00 0.04680 25000.00 12400.00 0.00 0.04698 24800.00 12600.00 0.00 0.04744 25000.00 12600.00 0.00 0.04760 24800.00 12800.00 0.00 0.04798 25000.00 12800.00 0.00 0.04813 24800.00 13000.00 0.00 0.04838 25000.00 13000.00 0.00 0.04853 24800.00 13200.00 0.00 0.04900 25000.00 13200.00 0.00 0.04917 24800.00 13400.00 0.00 0.04972 25000.00 13400.00 0.00 0.04987 24800.00 13600.00 0.00 0.05037 25000.00 13600.00 0.00 0.05050 24800.00 13800.00 0.00 0.05102 25000.00 13800.00 0.00 0.05117 24800.00 14000.00 0.00 0.05170 25000.00 14000.00 0.00 0.05185 24800.00 14200.00 0.00 0.05245 25000.00 14200.00 0.00 0.05258 24800.00 14400.00 0.00 0.05316 25000.00 14400.00 0.00 0.05328 24600.00 14600.00 0.00 0.05330 24800.00 14600.00 0.00 0.05396 25000.00 14600.00 0.00 0.05403 24600.00 14800.00 0.00 0.05409 24800.00 14800.00 0.00 0.05470 24600.00 15000.00 0.00 0.05481 24800.00 15000.00 0.00 0.05537 24600.00 15200.00 0.00 0.05556 24800.00 15200.00 0.00 0.05611 24600.00 15400.00 0.00 0.05636 24800.00 15400.00 0.00 0.05686 24600.00 15600.00 0.00 0.05721 24800.00 15600.00 0.00 0.05764 24600.00 15800.00 0.00 0.05813 24800.00 15800.00 0.00 0.05852 24600.00 16000.00 0.00 0.05934 24800.00 16000.00 0.00 0.05972 24600.00 16200.00 0.00 0.06055 24800.00 16200.00 0.00 0.06095 24600.00 16400.00 0.00 0.06165 24800.00 16400.00 0.00 0.06206 24600.00 16600.00 0.00 0.06270 24800.00 16600.00 0.00 0.06310 24600.00 16800.00 0.00 0.06374 24800.00 16800.00 0.00 0.06412 24600.00 17000.00 0.00 0.06475 24800.00 17000.00 0.00 0.06510 24600.00 17200.00 0.00 0.06560 24800.00 17200.00 0.00 0.06596 24600.00 17400.00 0.00 0.06661 24800.00 17400.00 0.00 0.06700 24600.00 17600.00 0.00 0.06771 24800.00 17600.00 0.00 0.06812 24600.00 17800.00 0.00 0.06879 24800.00 17800.00 0.00 0.06917 24600.00 18000.00 0.00 0.06992 24800.00 18000.00 0.00 0.07028 24600.00 18200.00 0.00 0.07108 24800.00 18200.00 0.00 0.07139 24600.00 18400.00 0.00 0.07221 24800.00 18400.00 0.00 0.07251 24600.00 18600.00 0.00 0.07347 24800.00 18600.00 0.00 0.07377 24600.00 18800.00 0.00 0.07479 24800.00 18800.00 0.00 0.07509 24600.00 19000.00 0.00 0.07610 24800.00 19000.00 0.00 0.07639 24600.00 19200.00 0.00 0.07753 24800.00 19200.00 0.00 0.07781 24600.00 19400.00 0.00 0.07891 24800.00 19400.00 0.00 0.07913 24600.00 19600.00 0.00 0.08018 24800.00 19600.00 0.00 0.08036 24600.00 19800.00 0.00 0.08144 24800.00 19800.00 0.00 0.08161 24400.00 20000.00 0.00 0.08172 24600.00 20000.00 0.00 0.08285 24800.00 20000.00 0.00 0.08298 24400.00 20200.00 0.00 0.08313 24600.00 20200.00 0.00 0.08424 24800.00 20200.00 0.00 0.08432 24400.00 20400.00 0.00 0.08453 24600.00 20400.00 0.00 0.08559 24800.00 20400.00 0.00 0.08563 24400.00 20600.00 0.00 0.08603 24600.00 20600.00 0.00 0.08706 24800.00 20600.00 0.00 0.08708 24400.00 20800.00 0.00 0.08737 24600.00 20800.00 0.00 0.08839 24800.00 20800.00 0.00 0.08842 24400.00 21000.00 0.00 0.08875 24600.00 21000.00 0.00 0.08982 24800.00 21000.00 0.00 0.08984 24400.00 21200.00 0.00 0.09035 24600.00 21200.00 0.00 0.09145 24400.00 21400.00 0.00 0.09213 24600.00 21400.00 0.00 0.09321 24400.00 21600.00 0.00 0.09382 24600.00 21600.00 0.00 0.09482 24400.00 21800.00 0.00 0.09547 24600.00 21800.00 0.00 0.09637 24400.00 22000.00 0.00 0.09711 24600.00 22000.00 0.00 0.09791 24400.00 22200.00 0.00 0.09865 24600.00 22200.00 0.00 0.09937 24400.00 22400.00 0.00 0.10031 24600.00 22400.00 0.00 0.10091 24400.00 22600.00 0.00 0.10167 24600.00 22600.00 0.00 0.10217 24400.00 22800.00 0.00 0.10312 24600.00 22800.00 0.00 0.10357 24400.00 23000.00 0.00 0.10465 24600.00 23000.00 0.00 0.10503 24400.00 23200.00 0.00 0.10621 24600.00 23200.00 0.00 0.10649 24400.00 23400.00 0.00 0.10773 24600.00 23400.00 0.00 0.10784 24400.00 23600.00 0.00 0.10901 24400.00 23800.00 0.00 0.11042 24400.00 24000.00 0.00 0.11154 24400.00 24200.00 0.00 0.11258 24400.00 24400.00 0.00 0.11284 24200.00 24600.00 30.00 0.11292 24200.00 24600.00 35.00 0.11304 24200.00 24600.00 40.00 0.11311 24200.00 24600.00 45.00 0.11313 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24253.92 24493.48 46.79 0.11326 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/284.ctf NX, NY, NZ: 512 512 1 MODE : real