CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/283.mrc Output diagnostic file name Micrographs_0601/283.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/283.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1736.000 8392.000 Mean, RMS : 4480.172 218.3184 TITLE 1: emi2mrc: 283.emi Sun Jan 05 21:57:06 2014 TITLE 2: kV 300, Mag 78000x, Def 24280, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 57.840, Y 597.587, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00950 10400.00 10000.00 0.00 -0.00947 13400.00 10000.00 0.00 -0.00946 13800.00 10000.00 0.00 -0.00934 14000.00 10000.00 0.00 -0.00913 14200.00 10000.00 0.00 -0.00904 14400.00 10000.00 0.00 -0.00890 14600.00 10000.00 0.00 -0.00877 14800.00 10000.00 0.00 -0.00857 15000.00 10000.00 0.00 -0.00826 15200.00 10000.00 0.00 -0.00809 15400.00 10000.00 0.00 -0.00796 15600.00 10000.00 0.00 -0.00762 15800.00 10000.00 0.00 -0.00713 16000.00 10000.00 0.00 -0.00675 16200.00 10000.00 0.00 -0.00642 16400.00 10000.00 0.00 -0.00599 16600.00 10000.00 0.00 -0.00554 16800.00 10000.00 0.00 -0.00514 17000.00 10000.00 0.00 -0.00466 17200.00 10000.00 0.00 -0.00409 17400.00 10000.00 0.00 -0.00367 17600.00 10000.00 0.00 -0.00320 17800.00 10000.00 0.00 -0.00261 18000.00 10000.00 0.00 -0.00204 18200.00 10000.00 0.00 -0.00133 18400.00 10000.00 0.00 -0.00049 18600.00 10000.00 0.00 0.00040 18800.00 10000.00 0.00 0.00139 19000.00 10000.00 0.00 0.00244 19200.00 10000.00 0.00 0.00349 19400.00 10000.00 0.00 0.00448 19600.00 10000.00 0.00 0.00546 19800.00 10000.00 0.00 0.00647 20000.00 10000.00 0.00 0.00742 20200.00 10000.00 0.00 0.00834 20400.00 10000.00 0.00 0.00933 20600.00 10000.00 0.00 0.01030 20800.00 10000.00 0.00 0.01119 21000.00 10000.00 0.00 0.01203 21200.00 10000.00 0.00 0.01290 21400.00 10000.00 0.00 0.01394 21600.00 10000.00 0.00 0.01507 21800.00 10000.00 0.00 0.01626 22000.00 10000.00 0.00 0.01759 22200.00 10000.00 0.00 0.01878 22400.00 10000.00 0.00 0.01997 22600.00 10000.00 0.00 0.02135 22800.00 10000.00 0.00 0.02269 23000.00 10000.00 0.00 0.02401 23200.00 10000.00 0.00 0.02532 23400.00 10000.00 0.00 0.02671 23600.00 10000.00 0.00 0.02814 23800.00 10000.00 0.00 0.02964 24000.00 10000.00 0.00 0.03135 24200.00 10000.00 0.00 0.03306 24400.00 10000.00 0.00 0.03481 24600.00 10000.00 0.00 0.03660 24800.00 10000.00 0.00 0.03796 25000.00 10000.00 0.00 0.03878 25200.00 10000.00 0.00 0.03929 25400.00 10000.00 0.00 0.03958 25600.00 10000.00 0.00 0.03978 25200.00 10200.00 0.00 0.03993 25400.00 10200.00 0.00 0.04020 25600.00 10200.00 0.00 0.04035 25200.00 10400.00 0.00 0.04053 25400.00 10400.00 0.00 0.04075 25600.00 10400.00 0.00 0.04088 25200.00 10600.00 0.00 0.04106 25400.00 10600.00 0.00 0.04130 25600.00 10600.00 0.00 0.04146 25200.00 10800.00 0.00 0.04160 25400.00 10800.00 0.00 0.04185 25600.00 10800.00 0.00 0.04201 25200.00 11000.00 0.00 0.04216 25400.00 11000.00 0.00 0.04241 25600.00 11000.00 0.00 0.04255 25200.00 11200.00 0.00 0.04273 25400.00 11200.00 0.00 0.04296 25600.00 11200.00 0.00 0.04309 25200.00 11400.00 0.00 0.04329 25400.00 11400.00 0.00 0.04352 25600.00 11400.00 0.00 0.04365 25200.00 11600.00 0.00 0.04384 25400.00 11600.00 0.00 0.04414 25600.00 11600.00 0.00 0.04430 25200.00 11800.00 0.00 0.04444 25400.00 11800.00 0.00 0.04476 25600.00 11800.00 0.00 0.04491 25200.00 12000.00 0.00 0.04499 25400.00 12000.00 0.00 0.04529 25600.00 12000.00 0.00 0.04538 25200.00 12200.00 0.00 0.04553 25400.00 12200.00 0.00 0.04584 25600.00 12200.00 0.00 0.04591 25200.00 12400.00 0.00 0.04613 25400.00 12400.00 0.00 0.04646 25600.00 12400.00 0.00 0.04653 25200.00 12600.00 0.00 0.04676 25400.00 12600.00 0.00 0.04709 25600.00 12600.00 0.00 0.04711 25200.00 12800.00 0.00 0.04740 25400.00 12800.00 0.00 0.04768 25200.00 13000.00 0.00 0.04796 25400.00 13000.00 0.00 0.04821 25200.00 13200.00 0.00 0.04854 25400.00 13200.00 0.00 0.04878 25200.00 13400.00 0.00 0.04921 25400.00 13400.00 0.00 0.04943 25200.00 13600.00 0.00 0.04996 25400.00 13600.00 0.00 0.05016 25200.00 13800.00 0.00 0.05053 25400.00 13800.00 0.00 0.05071 25200.00 14000.00 0.00 0.05099 25400.00 14000.00 0.00 0.05116 25200.00 14200.00 0.00 0.05158 25400.00 14200.00 0.00 0.05173 25200.00 14400.00 0.00 0.05212 25400.00 14400.00 0.00 0.05223 25200.00 14600.00 0.00 0.05268 25400.00 14600.00 0.00 0.05277 25000.00 14800.00 0.00 0.05278 25200.00 14800.00 0.00 0.05331 25400.00 14800.00 0.00 0.05338 25000.00 15000.00 0.00 0.05340 25200.00 15000.00 0.00 0.05396 25400.00 15000.00 0.00 0.05399 25000.00 15200.00 0.00 0.05403 25200.00 15200.00 0.00 0.05460 25000.00 15400.00 0.00 0.05472 25200.00 15400.00 0.00 0.05526 25000.00 15600.00 0.00 0.05555 25200.00 15600.00 0.00 0.05608 25000.00 15800.00 0.00 0.05630 25200.00 15800.00 0.00 0.05681 25000.00 16000.00 0.00 0.05703 25200.00 16000.00 0.00 0.05756 25400.00 16000.00 0.00 0.05756 25000.00 16200.00 0.00 0.05798 25200.00 16200.00 0.00 0.05852 25000.00 16400.00 0.00 0.05907 25200.00 16400.00 0.00 0.05959 25000.00 16600.00 0.00 0.06003 25200.00 16600.00 0.00 0.06047 25000.00 16800.00 0.00 0.06093 25200.00 16800.00 0.00 0.06133 25000.00 17000.00 0.00 0.06189 25200.00 17000.00 0.00 0.06229 25000.00 17200.00 0.00 0.06289 25200.00 17200.00 0.00 0.06324 25000.00 17400.00 0.00 0.06388 25200.00 17400.00 0.00 0.06415 25000.00 17600.00 0.00 0.06477 25200.00 17600.00 0.00 0.06501 25000.00 17800.00 0.00 0.06575 25200.00 17800.00 0.00 0.06600 25000.00 18000.00 0.00 0.06685 25200.00 18000.00 0.00 0.06711 24800.00 18200.00 0.00 0.06713 25000.00 18200.00 0.00 0.06810 25200.00 18200.00 0.00 0.06833 24800.00 18400.00 0.00 0.06835 25000.00 18400.00 0.00 0.06925 25200.00 18400.00 0.00 0.06944 24800.00 18600.00 0.00 0.06958 25000.00 18600.00 0.00 0.07045 25200.00 18600.00 0.00 0.07065 24800.00 18800.00 0.00 0.07089 25000.00 18800.00 0.00 0.07177 25200.00 18800.00 0.00 0.07203 24800.00 19000.00 0.00 0.07206 25000.00 19000.00 0.00 0.07297 25200.00 19000.00 0.00 0.07324 24800.00 19200.00 0.00 0.07338 25000.00 19200.00 0.00 0.07433 25200.00 19200.00 0.00 0.07461 24800.00 19400.00 0.00 0.07468 25000.00 19400.00 0.00 0.07567 25200.00 19400.00 0.00 0.07593 24800.00 19600.00 0.00 0.07603 25000.00 19600.00 0.00 0.07704 25200.00 19600.00 0.00 0.07727 24800.00 19800.00 0.00 0.07747 25000.00 19800.00 0.00 0.07850 25200.00 19800.00 0.00 0.07869 24800.00 20000.00 0.00 0.07896 25000.00 20000.00 0.00 0.07996 25200.00 20000.00 0.00 0.08008 24800.00 20200.00 0.00 0.08056 25000.00 20200.00 0.00 0.08149 25200.00 20200.00 0.00 0.08156 24800.00 20400.00 0.00 0.08204 25000.00 20400.00 0.00 0.08294 25200.00 20400.00 0.00 0.08301 24800.00 20600.00 0.00 0.08360 25000.00 20600.00 0.00 0.08449 25200.00 20600.00 0.00 0.08456 24800.00 20800.00 0.00 0.08510 25000.00 20800.00 0.00 0.08596 25200.00 20800.00 0.00 0.08599 24800.00 21000.00 0.00 0.08654 25000.00 21000.00 0.00 0.08738 24800.00 21200.00 0.00 0.08813 25000.00 21200.00 0.00 0.08895 24800.00 21400.00 0.00 0.08973 25000.00 21400.00 0.00 0.09053 24800.00 21600.00 0.00 0.09131 25000.00 21600.00 0.00 0.09211 24800.00 21800.00 0.00 0.09305 25000.00 21800.00 0.00 0.09381 24800.00 22000.00 0.00 0.09498 25000.00 22000.00 0.00 0.09569 24800.00 22200.00 0.00 0.09695 25000.00 22200.00 0.00 0.09755 24800.00 22400.00 0.00 0.09874 25000.00 22400.00 0.00 0.09920 24800.00 22600.00 0.00 0.10026 25000.00 22600.00 0.00 0.10062 24800.00 22800.00 0.00 0.10192 25000.00 22800.00 0.00 0.10224 24800.00 23000.00 0.00 0.10358 25000.00 23000.00 0.00 0.10383 24800.00 23200.00 0.00 0.10533 25000.00 23200.00 0.00 0.10549 24600.00 23400.00 0.00 0.10563 24800.00 23400.00 0.00 0.10725 25000.00 23400.00 0.00 0.10728 24600.00 23600.00 0.00 0.10752 24800.00 23600.00 0.00 0.10909 24600.00 23800.00 0.00 0.10954 24800.00 23800.00 0.00 0.11104 24600.00 24000.00 0.00 0.11148 24800.00 24000.00 0.00 0.11287 24600.00 24200.00 0.00 0.11347 24800.00 24200.00 0.00 0.11465 24600.00 24400.00 0.00 0.11508 24800.00 24400.00 0.00 0.11599 24600.00 24600.00 0.00 0.11642 24800.00 24600.00 0.00 0.11696 24600.00 24800.00 0.00 0.11702 24600.00 24800.00 5.00 0.11716 24600.00 24800.00 10.00 0.11729 24600.00 24800.00 15.00 0.11741 24600.00 24800.00 20.00 0.11751 24600.00 24800.00 25.00 0.11760 24600.00 24800.00 30.00 0.11766 24600.00 24800.00 35.00 0.11770 24600.00 24800.00 40.00 0.11772 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24607.55 24823.72 40.00 0.11774 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/283.ctf NX, NY, NZ: 512 512 1 MODE : real