CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/282.mrc Output diagnostic file name Micrographs_0601/282.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/282.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2494.000 7876.000 Mean, RMS : 4476.376 221.2358 TITLE 1: emi2mrc: 282.emi Sun Jan 05 21:56:25 2014 TITLE 2: kV 300, Mag 78000x, Def 24280, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 60.280, Y 597.344, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01055 15400.00 10000.00 0.00 -0.01032 15600.00 10000.00 0.00 -0.00990 15800.00 10000.00 0.00 -0.00953 16000.00 10000.00 0.00 -0.00917 16200.00 10000.00 0.00 -0.00865 16400.00 10000.00 0.00 -0.00813 16600.00 10000.00 0.00 -0.00769 16800.00 10000.00 0.00 -0.00731 17000.00 10000.00 0.00 -0.00687 17200.00 10000.00 0.00 -0.00627 17400.00 10000.00 0.00 -0.00564 17600.00 10000.00 0.00 -0.00508 17800.00 10000.00 0.00 -0.00447 18000.00 10000.00 0.00 -0.00375 18200.00 10000.00 0.00 -0.00299 18400.00 10000.00 0.00 -0.00206 18600.00 10000.00 0.00 -0.00113 18800.00 10000.00 0.00 -0.00022 19000.00 10000.00 0.00 0.00073 19200.00 10000.00 0.00 0.00154 19400.00 10000.00 0.00 0.00218 19600.00 10000.00 0.00 0.00290 19800.00 10000.00 0.00 0.00385 20000.00 10000.00 0.00 0.00474 20200.00 10000.00 0.00 0.00559 20400.00 10000.00 0.00 0.00654 20600.00 10000.00 0.00 0.00757 20800.00 10000.00 0.00 0.00859 21000.00 10000.00 0.00 0.00945 21200.00 10000.00 0.00 0.01031 21400.00 10000.00 0.00 0.01115 21600.00 10000.00 0.00 0.01196 21800.00 10000.00 0.00 0.01290 22000.00 10000.00 0.00 0.01388 22200.00 10000.00 0.00 0.01486 22400.00 10000.00 0.00 0.01585 22600.00 10000.00 0.00 0.01675 22800.00 10000.00 0.00 0.01760 23000.00 10000.00 0.00 0.01872 23200.00 10000.00 0.00 0.02002 23400.00 10000.00 0.00 0.02123 23600.00 10000.00 0.00 0.02244 23800.00 10000.00 0.00 0.02377 24000.00 10000.00 0.00 0.02517 24200.00 10000.00 0.00 0.02664 24400.00 10000.00 0.00 0.02838 24600.00 10000.00 0.00 0.03041 24800.00 10000.00 0.00 0.03249 25000.00 10000.00 0.00 0.03421 25200.00 10000.00 0.00 0.03528 25400.00 10000.00 0.00 0.03591 25600.00 10000.00 0.00 0.03629 25800.00 10000.00 0.00 0.03643 25400.00 10200.00 0.00 0.03651 25600.00 10200.00 0.00 0.03689 25800.00 10200.00 0.00 0.03704 25400.00 10400.00 0.00 0.03710 25600.00 10400.00 0.00 0.03748 25800.00 10400.00 0.00 0.03762 25600.00 10600.00 0.00 0.03796 25800.00 10600.00 0.00 0.03808 25600.00 10800.00 0.00 0.03830 25800.00 10800.00 0.00 0.03840 25600.00 11000.00 0.00 0.03860 25800.00 11000.00 0.00 0.03868 25600.00 11200.00 0.00 0.03892 25800.00 11200.00 0.00 0.03899 25400.00 11400.00 0.00 0.03906 25600.00 11400.00 0.00 0.03938 25800.00 11400.00 0.00 0.03947 25400.00 11600.00 0.00 0.03950 25600.00 11600.00 0.00 0.03986 25800.00 11600.00 0.00 0.03998 25400.00 11800.00 0.00 0.04007 25600.00 11800.00 0.00 0.04043 25800.00 11800.00 0.00 0.04052 25400.00 12000.00 0.00 0.04063 25600.00 12000.00 0.00 0.04095 25800.00 12000.00 0.00 0.04101 25600.00 12200.00 0.00 0.04128 25800.00 12200.00 0.00 0.04134 25400.00 12400.00 0.00 0.04138 25600.00 12400.00 0.00 0.04169 25800.00 12400.00 0.00 0.04178 25400.00 12600.00 0.00 0.04187 25600.00 12600.00 0.00 0.04217 25800.00 12600.00 0.00 0.04225 25400.00 12800.00 0.00 0.04239 25600.00 12800.00 0.00 0.04268 25800.00 12800.00 0.00 0.04275 25400.00 13000.00 0.00 0.04287 25600.00 13000.00 0.00 0.04318 25800.00 13000.00 0.00 0.04328 25400.00 13200.00 0.00 0.04343 25600.00 13200.00 0.00 0.04378 25800.00 13200.00 0.00 0.04388 25400.00 13400.00 0.00 0.04406 25600.00 13400.00 0.00 0.04444 25800.00 13400.00 0.00 0.04451 25400.00 13600.00 0.00 0.04473 25600.00 13600.00 0.00 0.04510 25800.00 13600.00 0.00 0.04513 25400.00 13800.00 0.00 0.04544 25600.00 13800.00 0.00 0.04577 25400.00 14000.00 0.00 0.04613 25600.00 14000.00 0.00 0.04644 25400.00 14200.00 0.00 0.04680 25600.00 14200.00 0.00 0.04710 25400.00 14400.00 0.00 0.04727 25600.00 14400.00 0.00 0.04753 25400.00 14600.00 0.00 0.04776 25600.00 14600.00 0.00 0.04803 25400.00 14800.00 0.00 0.04855 25600.00 14800.00 0.00 0.04882 25200.00 15000.00 0.00 0.04887 25400.00 15000.00 0.00 0.04945 25600.00 15000.00 0.00 0.04969 25400.00 15200.00 0.00 0.05025 25600.00 15200.00 0.00 0.05046 25200.00 15400.00 0.00 0.05047 25400.00 15400.00 0.00 0.05105 25600.00 15400.00 0.00 0.05124 25200.00 15600.00 0.00 0.05132 25400.00 15600.00 0.00 0.05188 25600.00 15600.00 0.00 0.05205 25200.00 15800.00 0.00 0.05215 25400.00 15800.00 0.00 0.05269 25600.00 15800.00 0.00 0.05283 25200.00 16000.00 0.00 0.05304 25400.00 16000.00 0.00 0.05352 25600.00 16000.00 0.00 0.05362 25200.00 16200.00 0.00 0.05379 25400.00 16200.00 0.00 0.05424 25600.00 16200.00 0.00 0.05433 25200.00 16400.00 0.00 0.05460 25400.00 16400.00 0.00 0.05507 25600.00 16400.00 0.00 0.05518 25200.00 16600.00 0.00 0.05552 25400.00 16600.00 0.00 0.05601 25600.00 16600.00 0.00 0.05610 25200.00 16800.00 0.00 0.05642 25400.00 16800.00 0.00 0.05690 25600.00 16800.00 0.00 0.05695 25200.00 17000.00 0.00 0.05748 25400.00 17000.00 0.00 0.05794 25600.00 17000.00 0.00 0.05798 25200.00 17200.00 0.00 0.05862 25400.00 17200.00 0.00 0.05905 25600.00 17200.00 0.00 0.05909 25200.00 17400.00 0.00 0.05972 25400.00 17400.00 0.00 0.06011 25600.00 17400.00 0.00 0.06015 25200.00 17600.00 0.00 0.06082 25400.00 17600.00 0.00 0.06125 25600.00 17600.00 0.00 0.06133 25200.00 17800.00 0.00 0.06199 25400.00 17800.00 0.00 0.06246 25600.00 17800.00 0.00 0.06255 25200.00 18000.00 0.00 0.06310 25400.00 18000.00 0.00 0.06356 25600.00 18000.00 0.00 0.06359 25200.00 18200.00 0.00 0.06421 25400.00 18200.00 0.00 0.06462 25200.00 18400.00 0.00 0.06533 25400.00 18400.00 0.00 0.06571 25200.00 18600.00 0.00 0.06654 25400.00 18600.00 0.00 0.06690 25200.00 18800.00 0.00 0.06787 25400.00 18800.00 0.00 0.06818 25200.00 19000.00 0.00 0.06920 25400.00 19000.00 0.00 0.06946 25000.00 19200.00 0.00 0.06957 25200.00 19200.00 0.00 0.07063 25400.00 19200.00 0.00 0.07089 25000.00 19400.00 0.00 0.07114 25200.00 19400.00 0.00 0.07218 25400.00 19400.00 0.00 0.07241 25000.00 19600.00 0.00 0.07271 25200.00 19600.00 0.00 0.07369 25400.00 19600.00 0.00 0.07390 25000.00 19800.00 0.00 0.07403 25200.00 19800.00 0.00 0.07501 25400.00 19800.00 0.00 0.07520 25000.00 20000.00 0.00 0.07536 25200.00 20000.00 0.00 0.07632 25400.00 20000.00 0.00 0.07651 25000.00 20200.00 0.00 0.07664 25200.00 20200.00 0.00 0.07761 25400.00 20200.00 0.00 0.07778 25000.00 20400.00 0.00 0.07784 25200.00 20400.00 0.00 0.07885 25400.00 20400.00 0.00 0.07902 25000.00 20600.00 0.00 0.07924 25200.00 20600.00 0.00 0.08027 25400.00 20600.00 0.00 0.08039 25000.00 20800.00 0.00 0.08070 25200.00 20800.00 0.00 0.08168 25400.00 20800.00 0.00 0.08173 25000.00 21000.00 0.00 0.08232 25200.00 21000.00 0.00 0.08324 25000.00 21200.00 0.00 0.08393 25200.00 21200.00 0.00 0.08481 25000.00 21400.00 0.00 0.08553 25200.00 21400.00 0.00 0.08633 25000.00 21600.00 0.00 0.08705 25200.00 21600.00 0.00 0.08778 25000.00 21800.00 0.00 0.08852 25200.00 21800.00 0.00 0.08926 25000.00 22000.00 0.00 0.09013 25200.00 22000.00 0.00 0.09090 25000.00 22200.00 0.00 0.09184 25200.00 22200.00 0.00 0.09263 25000.00 22400.00 0.00 0.09370 25200.00 22400.00 0.00 0.09444 25000.00 22600.00 0.00 0.09545 25200.00 22600.00 0.00 0.09609 25000.00 22800.00 0.00 0.09741 25200.00 22800.00 0.00 0.09794 25000.00 23000.00 0.00 0.09936 25200.00 23000.00 0.00 0.09979 25000.00 23200.00 0.00 0.10135 25200.00 23200.00 0.00 0.10171 25000.00 23400.00 0.00 0.10357 25200.00 23400.00 0.00 0.10385 24800.00 23600.00 0.00 0.10389 25000.00 23600.00 0.00 0.10576 25200.00 23600.00 0.00 0.10592 24800.00 23800.00 0.00 0.10647 25000.00 23800.00 0.00 0.10829 24800.00 24000.00 0.00 0.10893 25000.00 24000.00 0.00 0.11064 24800.00 24200.00 0.00 0.11137 25000.00 24200.00 0.00 0.11291 24800.00 24400.00 0.00 0.11364 25000.00 24400.00 0.00 0.11497 24800.00 24600.00 0.00 0.11587 25000.00 24600.00 0.00 0.11684 24800.00 24800.00 0.00 0.11804 25000.00 24800.00 0.00 0.11833 24800.00 25000.00 0.00 0.11849 24800.00 25000.00 5.00 0.11866 24800.00 25000.00 10.00 0.11882 24800.00 25000.00 15.00 0.11897 24800.00 25000.00 20.00 0.11910 24800.00 25000.00 25.00 0.11922 24800.00 25000.00 30.00 0.11930 24800.00 25000.00 35.00 0.11935 24800.00 25000.00 40.00 0.11937 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24778.48 25018.10 40.00 0.11939 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/282.ctf NX, NY, NZ: 512 512 1 MODE : real