CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/281.mrc Output diagnostic file name Micrographs_0601/281.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/281.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2432.000 8354.000 Mean, RMS : 4481.395 223.2219 TITLE 1: emi2mrc: 281.emi Sun Jan 05 21:55:47 2014 TITLE 2: kV 300, Mag 78000x, Def 24280, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 63.010, Y 596.725, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00998 13000.00 10000.00 0.00 -0.00975 13200.00 10000.00 0.00 -0.00952 13400.00 10000.00 0.00 -0.00933 13600.00 10000.00 0.00 -0.00909 13800.00 10000.00 0.00 -0.00897 14000.00 10000.00 0.00 -0.00895 14400.00 10000.00 0.00 -0.00890 14600.00 10000.00 0.00 -0.00875 14800.00 10000.00 0.00 -0.00854 15000.00 10000.00 0.00 -0.00821 15200.00 10000.00 0.00 -0.00788 15400.00 10000.00 0.00 -0.00765 15600.00 10000.00 0.00 -0.00744 15800.00 10000.00 0.00 -0.00703 16000.00 10000.00 0.00 -0.00647 16200.00 10000.00 0.00 -0.00583 16400.00 10000.00 0.00 -0.00518 16600.00 10000.00 0.00 -0.00467 16800.00 10000.00 0.00 -0.00405 17000.00 10000.00 0.00 -0.00325 17200.00 10000.00 0.00 -0.00246 17400.00 10000.00 0.00 -0.00170 17600.00 10000.00 0.00 -0.00098 17800.00 10000.00 0.00 -0.00032 18000.00 10000.00 0.00 0.00029 18200.00 10000.00 0.00 0.00100 18400.00 10000.00 0.00 0.00177 18600.00 10000.00 0.00 0.00249 18800.00 10000.00 0.00 0.00316 19000.00 10000.00 0.00 0.00378 19200.00 10000.00 0.00 0.00447 19400.00 10000.00 0.00 0.00537 19600.00 10000.00 0.00 0.00631 19800.00 10000.00 0.00 0.00709 20000.00 10000.00 0.00 0.00773 20200.00 10000.00 0.00 0.00847 20400.00 10000.00 0.00 0.00921 20600.00 10000.00 0.00 0.00995 20800.00 10000.00 0.00 0.01076 21000.00 10000.00 0.00 0.01169 21200.00 10000.00 0.00 0.01274 21400.00 10000.00 0.00 0.01370 21600.00 10000.00 0.00 0.01466 21800.00 10000.00 0.00 0.01581 22000.00 10000.00 0.00 0.01699 22200.00 10000.00 0.00 0.01816 22400.00 10000.00 0.00 0.01939 22600.00 10000.00 0.00 0.02062 22800.00 10000.00 0.00 0.02193 23000.00 10000.00 0.00 0.02346 23200.00 10000.00 0.00 0.02503 23400.00 10000.00 0.00 0.02657 23600.00 10000.00 0.00 0.02822 23800.00 10000.00 0.00 0.02998 24000.00 10000.00 0.00 0.03178 24200.00 10000.00 0.00 0.03366 24400.00 10000.00 0.00 0.03561 24600.00 10000.00 0.00 0.03742 24800.00 10000.00 0.00 0.03867 25000.00 10000.00 0.00 0.03917 25200.00 10000.00 0.00 0.03928 25000.00 10200.00 0.00 0.03950 25200.00 10200.00 0.00 0.03961 25000.00 10400.00 0.00 0.03979 25200.00 10400.00 0.00 0.03990 25000.00 10600.00 0.00 0.04013 25200.00 10600.00 0.00 0.04025 25000.00 10800.00 0.00 0.04061 25200.00 10800.00 0.00 0.04076 25400.00 10800.00 0.00 0.04077 25000.00 11000.00 0.00 0.04127 25200.00 11000.00 0.00 0.04145 25400.00 11000.00 0.00 0.04147 25000.00 11200.00 0.00 0.04179 25200.00 11200.00 0.00 0.04196 25400.00 11200.00 0.00 0.04199 25600.00 11200.00 0.00 0.04201 25800.00 11200.00 0.00 0.04201 25000.00 11400.00 0.00 0.04223 25200.00 11400.00 0.00 0.04244 25400.00 11400.00 0.00 0.04252 25600.00 11400.00 0.00 0.04259 25000.00 11600.00 0.00 0.04275 25200.00 11600.00 0.00 0.04300 25400.00 11600.00 0.00 0.04314 25600.00 11600.00 0.00 0.04322 25000.00 11800.00 0.00 0.04331 25200.00 11800.00 0.00 0.04360 25400.00 11800.00 0.00 0.04375 25600.00 11800.00 0.00 0.04378 25000.00 12000.00 0.00 0.04388 25200.00 12000.00 0.00 0.04417 25400.00 12000.00 0.00 0.04430 25000.00 12200.00 0.00 0.04441 25200.00 12200.00 0.00 0.04470 25400.00 12200.00 0.00 0.04482 25000.00 12400.00 0.00 0.04503 25200.00 12400.00 0.00 0.04533 25400.00 12400.00 0.00 0.04546 25000.00 12600.00 0.00 0.04570 25200.00 12600.00 0.00 0.04602 25400.00 12600.00 0.00 0.04617 25000.00 12800.00 0.00 0.04645 25200.00 12800.00 0.00 0.04678 25400.00 12800.00 0.00 0.04692 25000.00 13000.00 0.00 0.04721 25200.00 13000.00 0.00 0.04752 25400.00 13000.00 0.00 0.04764 25000.00 13200.00 0.00 0.04784 25200.00 13200.00 0.00 0.04815 25400.00 13200.00 0.00 0.04828 25000.00 13400.00 0.00 0.04849 25200.00 13400.00 0.00 0.04885 25400.00 13400.00 0.00 0.04902 25000.00 13600.00 0.00 0.04919 25200.00 13600.00 0.00 0.04960 25400.00 13600.00 0.00 0.04974 25000.00 13800.00 0.00 0.04993 25200.00 13800.00 0.00 0.05033 25400.00 13800.00 0.00 0.05036 25000.00 14000.00 0.00 0.05072 25200.00 14000.00 0.00 0.05102 25000.00 14200.00 0.00 0.05130 25200.00 14200.00 0.00 0.05151 25000.00 14400.00 0.00 0.05181 25200.00 14400.00 0.00 0.05199 25000.00 14600.00 0.00 0.05246 25200.00 14600.00 0.00 0.05259 25000.00 14800.00 0.00 0.05307 25200.00 14800.00 0.00 0.05316 25000.00 15000.00 0.00 0.05377 25200.00 15000.00 0.00 0.05383 25000.00 15200.00 0.00 0.05439 25200.00 15200.00 0.00 0.05446 25000.00 15400.00 0.00 0.05502 25200.00 15400.00 0.00 0.05509 24800.00 15600.00 0.00 0.05519 25000.00 15600.00 0.00 0.05579 25200.00 15600.00 0.00 0.05585 24800.00 15800.00 0.00 0.05598 25000.00 15800.00 0.00 0.05657 25200.00 15800.00 0.00 0.05660 24800.00 16000.00 0.00 0.05677 25000.00 16000.00 0.00 0.05738 25200.00 16000.00 0.00 0.05743 24800.00 16200.00 0.00 0.05758 25000.00 16200.00 0.00 0.05822 25200.00 16200.00 0.00 0.05832 24800.00 16400.00 0.00 0.05859 25000.00 16400.00 0.00 0.05925 25200.00 16400.00 0.00 0.05936 24800.00 16600.00 0.00 0.05958 25000.00 16600.00 0.00 0.06026 25200.00 16600.00 0.00 0.06035 24800.00 16800.00 0.00 0.06056 25000.00 16800.00 0.00 0.06123 25200.00 16800.00 0.00 0.06130 24800.00 17000.00 0.00 0.06171 25000.00 17000.00 0.00 0.06236 25200.00 17000.00 0.00 0.06241 24800.00 17200.00 0.00 0.06290 25000.00 17200.00 0.00 0.06354 25200.00 17200.00 0.00 0.06358 24800.00 17400.00 0.00 0.06414 25000.00 17400.00 0.00 0.06477 25200.00 17400.00 0.00 0.06481 24800.00 17600.00 0.00 0.06537 25000.00 17600.00 0.00 0.06598 25200.00 17600.00 0.00 0.06599 24800.00 17800.00 0.00 0.06664 25000.00 17800.00 0.00 0.06722 24800.00 18000.00 0.00 0.06797 25000.00 18000.00 0.00 0.06851 24800.00 18200.00 0.00 0.06934 25000.00 18200.00 0.00 0.06983 24800.00 18400.00 0.00 0.07062 25000.00 18400.00 0.00 0.07105 24800.00 18600.00 0.00 0.07192 25000.00 18600.00 0.00 0.07233 24800.00 18800.00 0.00 0.07330 25000.00 18800.00 0.00 0.07372 24800.00 19000.00 0.00 0.07473 25000.00 19000.00 0.00 0.07519 24800.00 19200.00 0.00 0.07622 25000.00 19200.00 0.00 0.07667 24800.00 19400.00 0.00 0.07755 25000.00 19400.00 0.00 0.07800 24800.00 19600.00 0.00 0.07903 25000.00 19600.00 0.00 0.07946 24800.00 19800.00 0.00 0.08052 25000.00 19800.00 0.00 0.08091 24800.00 20000.00 0.00 0.08201 25000.00 20000.00 0.00 0.08236 24800.00 20200.00 0.00 0.08353 25000.00 20200.00 0.00 0.08387 24800.00 20400.00 0.00 0.08510 25000.00 20400.00 0.00 0.08541 24800.00 20600.00 0.00 0.08674 25000.00 20600.00 0.00 0.08703 24800.00 20800.00 0.00 0.08821 25000.00 20800.00 0.00 0.08850 24800.00 21000.00 0.00 0.08977 25000.00 21000.00 0.00 0.09003 24800.00 21200.00 0.00 0.09124 25000.00 21200.00 0.00 0.09144 24800.00 21400.00 0.00 0.09272 25000.00 21400.00 0.00 0.09287 24800.00 21600.00 0.00 0.09440 25000.00 21600.00 0.00 0.09448 24600.00 21800.00 0.00 0.09454 24800.00 21800.00 0.00 0.09621 24600.00 22000.00 0.00 0.09656 24800.00 22000.00 0.00 0.09816 24600.00 22200.00 0.00 0.09855 24800.00 22200.00 0.00 0.10012 24600.00 22400.00 0.00 0.10077 24800.00 22400.00 0.00 0.10235 24600.00 22600.00 0.00 0.10302 24800.00 22600.00 0.00 0.10457 24600.00 22800.00 0.00 0.10544 24800.00 22800.00 0.00 0.10694 24600.00 23000.00 0.00 0.10794 24800.00 23000.00 0.00 0.10938 24600.00 23200.00 0.00 0.11058 24800.00 23200.00 0.00 0.11190 24600.00 23400.00 0.00 0.11336 24800.00 23400.00 0.00 0.11451 24600.00 23600.00 0.00 0.11598 24800.00 23600.00 0.00 0.11700 24600.00 23800.00 0.00 0.11898 24800.00 23800.00 0.00 0.11986 24600.00 24000.00 0.00 0.12207 24800.00 24000.00 0.00 0.12268 24600.00 24200.00 0.00 0.12517 24800.00 24200.00 0.00 0.12535 24400.00 24400.00 0.00 0.12572 24600.00 24400.00 0.00 0.12784 24600.00 24600.00 0.00 0.12949 24600.00 24800.00 20.00 0.12957 24600.00 24800.00 25.00 0.12971 24400.00 24800.00 30.00 0.12974 24600.00 24800.00 30.00 0.12980 24400.00 24800.00 35.00 0.12989 24400.00 24800.00 40.00 0.12997 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24514.06 24756.25 41.81 0.13038 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/281.ctf NX, NY, NZ: 512 512 1 MODE : real