CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/280.mrc Output diagnostic file name Micrographs_0601/280.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/280.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2458.000 7594.000 Mean, RMS : 4485.932 219.1189 TITLE 1: emi2mrc: 280.emi Sun Jan 05 21:53:33 2014 TITLE 2: kV 300, Mag 78000x, Def 24280, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 65.774, Y 596.383, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00910 10400.00 10000.00 0.00 -0.00908 10600.00 10000.00 0.00 -0.00904 11400.00 10000.00 0.00 -0.00902 13400.00 10000.00 0.00 -0.00885 13600.00 10000.00 0.00 -0.00871 13800.00 10000.00 0.00 -0.00867 14000.00 10000.00 0.00 -0.00860 14200.00 10000.00 0.00 -0.00856 14400.00 10000.00 0.00 -0.00849 14600.00 10000.00 0.00 -0.00815 14800.00 10000.00 0.00 -0.00769 15000.00 10000.00 0.00 -0.00710 15200.00 10000.00 0.00 -0.00640 15400.00 10000.00 0.00 -0.00576 15600.00 10000.00 0.00 -0.00506 15800.00 10000.00 0.00 -0.00437 16000.00 10000.00 0.00 -0.00380 16200.00 10000.00 0.00 -0.00327 16400.00 10000.00 0.00 -0.00290 16600.00 10000.00 0.00 -0.00259 16800.00 10000.00 0.00 -0.00213 17000.00 10000.00 0.00 -0.00153 17200.00 10000.00 0.00 -0.00083 17400.00 10000.00 0.00 -0.00015 17600.00 10000.00 0.00 0.00049 17800.00 10000.00 0.00 0.00126 18000.00 10000.00 0.00 0.00211 18200.00 10000.00 0.00 0.00285 18400.00 10000.00 0.00 0.00353 18600.00 10000.00 0.00 0.00448 18800.00 10000.00 0.00 0.00556 19000.00 10000.00 0.00 0.00656 19200.00 10000.00 0.00 0.00749 19400.00 10000.00 0.00 0.00823 19600.00 10000.00 0.00 0.00897 19800.00 10000.00 0.00 0.00978 20000.00 10000.00 0.00 0.01060 20200.00 10000.00 0.00 0.01158 20400.00 10000.00 0.00 0.01259 20600.00 10000.00 0.00 0.01351 20800.00 10000.00 0.00 0.01439 21000.00 10000.00 0.00 0.01531 21200.00 10000.00 0.00 0.01639 21400.00 10000.00 0.00 0.01760 21600.00 10000.00 0.00 0.01884 21800.00 10000.00 0.00 0.02011 22000.00 10000.00 0.00 0.02151 22200.00 10000.00 0.00 0.02303 22400.00 10000.00 0.00 0.02455 22600.00 10000.00 0.00 0.02590 22800.00 10000.00 0.00 0.02719 23000.00 10000.00 0.00 0.02851 23200.00 10000.00 0.00 0.02985 23400.00 10000.00 0.00 0.03151 23600.00 10000.00 0.00 0.03341 23800.00 10000.00 0.00 0.03518 24000.00 10000.00 0.00 0.03670 24200.00 10000.00 0.00 0.03798 24400.00 10000.00 0.00 0.03881 24600.00 10000.00 0.00 0.03926 24800.00 10000.00 0.00 0.03958 25000.00 10000.00 0.00 0.03975 25200.00 10000.00 0.00 0.03984 24800.00 10200.00 0.00 0.03985 25000.00 10200.00 0.00 0.04003 25200.00 10200.00 0.00 0.04009 24800.00 10400.00 0.00 0.04026 25000.00 10400.00 0.00 0.04044 25200.00 10400.00 0.00 0.04049 24800.00 10600.00 0.00 0.04081 25000.00 10600.00 0.00 0.04099 25200.00 10600.00 0.00 0.04105 24800.00 10800.00 0.00 0.04136 25000.00 10800.00 0.00 0.04152 25200.00 10800.00 0.00 0.04155 24800.00 11000.00 0.00 0.04182 25000.00 11000.00 0.00 0.04197 25200.00 11000.00 0.00 0.04201 24800.00 11200.00 0.00 0.04232 25000.00 11200.00 0.00 0.04249 25200.00 11200.00 0.00 0.04256 24600.00 11400.00 0.00 0.04261 24800.00 11400.00 0.00 0.04294 25000.00 11400.00 0.00 0.04311 25200.00 11400.00 0.00 0.04318 24600.00 11600.00 0.00 0.04323 24800.00 11600.00 0.00 0.04355 25000.00 11600.00 0.00 0.04372 25200.00 11600.00 0.00 0.04378 24600.00 11800.00 0.00 0.04384 24800.00 11800.00 0.00 0.04417 25000.00 11800.00 0.00 0.04434 25200.00 11800.00 0.00 0.04441 24600.00 12000.00 0.00 0.04442 24800.00 12000.00 0.00 0.04474 25000.00 12000.00 0.00 0.04490 25200.00 12000.00 0.00 0.04496 24600.00 12200.00 0.00 0.04503 24800.00 12200.00 0.00 0.04534 25000.00 12200.00 0.00 0.04549 25200.00 12200.00 0.00 0.04554 24600.00 12400.00 0.00 0.04554 24800.00 12400.00 0.00 0.04585 25000.00 12400.00 0.00 0.04599 25200.00 12400.00 0.00 0.04602 24800.00 12600.00 0.00 0.04623 25000.00 12600.00 0.00 0.04638 25200.00 12600.00 0.00 0.04642 24600.00 12800.00 0.00 0.04642 24800.00 12800.00 0.00 0.04679 25000.00 12800.00 0.00 0.04696 25200.00 12800.00 0.00 0.04700 24800.00 13000.00 0.00 0.04734 25000.00 13000.00 0.00 0.04750 25200.00 13000.00 0.00 0.04755 24600.00 13200.00 0.00 0.04764 24800.00 13200.00 0.00 0.04804 25000.00 13200.00 0.00 0.04819 25200.00 13200.00 0.00 0.04827 24600.00 13400.00 0.00 0.04849 24800.00 13400.00 0.00 0.04887 25000.00 13400.00 0.00 0.04904 25200.00 13400.00 0.00 0.04914 24600.00 13600.00 0.00 0.04933 24800.00 13600.00 0.00 0.04973 25000.00 13600.00 0.00 0.04993 25200.00 13600.00 0.00 0.05001 24600.00 13800.00 0.00 0.05032 24800.00 13800.00 0.00 0.05075 25000.00 13800.00 0.00 0.05094 25200.00 13800.00 0.00 0.05095 24600.00 14000.00 0.00 0.05127 24800.00 14000.00 0.00 0.05167 25000.00 14000.00 0.00 0.05180 24600.00 14200.00 0.00 0.05210 24800.00 14200.00 0.00 0.05250 25000.00 14200.00 0.00 0.05261 24600.00 14400.00 0.00 0.05297 24800.00 14400.00 0.00 0.05339 25000.00 14400.00 0.00 0.05347 24600.00 14600.00 0.00 0.05383 24800.00 14600.00 0.00 0.05421 24600.00 14800.00 0.00 0.05456 24800.00 14800.00 0.00 0.05484 24600.00 15000.00 0.00 0.05530 24800.00 15000.00 0.00 0.05549 24400.00 15200.00 0.00 0.05568 24600.00 15200.00 0.00 0.05610 24800.00 15200.00 0.00 0.05626 24400.00 15400.00 0.00 0.05656 24600.00 15400.00 0.00 0.05698 24800.00 15400.00 0.00 0.05718 24400.00 15600.00 0.00 0.05749 24600.00 15600.00 0.00 0.05794 24800.00 15600.00 0.00 0.05814 24400.00 15800.00 0.00 0.05847 24600.00 15800.00 0.00 0.05893 24800.00 15800.00 0.00 0.05911 24400.00 16000.00 0.00 0.05953 24600.00 16000.00 0.00 0.05997 24800.00 16000.00 0.00 0.06011 24400.00 16200.00 0.00 0.06047 24600.00 16200.00 0.00 0.06085 24800.00 16200.00 0.00 0.06093 24400.00 16400.00 0.00 0.06148 24600.00 16400.00 0.00 0.06180 24800.00 16400.00 0.00 0.06184 24400.00 16600.00 0.00 0.06263 24600.00 16600.00 0.00 0.06291 24800.00 16600.00 0.00 0.06295 24200.00 16800.00 0.00 0.06297 24400.00 16800.00 0.00 0.06384 24600.00 16800.00 0.00 0.06410 24800.00 16800.00 0.00 0.06416 24200.00 17000.00 0.00 0.06421 24400.00 17000.00 0.00 0.06506 24600.00 17000.00 0.00 0.06532 24800.00 17000.00 0.00 0.06538 24200.00 17200.00 0.00 0.06548 24400.00 17200.00 0.00 0.06632 24600.00 17200.00 0.00 0.06658 24800.00 17200.00 0.00 0.06662 24200.00 17400.00 0.00 0.06682 24400.00 17400.00 0.00 0.06764 24600.00 17400.00 0.00 0.06789 24800.00 17400.00 0.00 0.06790 24200.00 17600.00 0.00 0.06811 24400.00 17600.00 0.00 0.06891 24600.00 17600.00 0.00 0.06914 24200.00 17800.00 0.00 0.06956 24400.00 17800.00 0.00 0.07032 24600.00 17800.00 0.00 0.07052 24200.00 18000.00 0.00 0.07092 24400.00 18000.00 0.00 0.07163 24600.00 18000.00 0.00 0.07178 24200.00 18200.00 0.00 0.07235 24400.00 18200.00 0.00 0.07304 24600.00 18200.00 0.00 0.07317 24200.00 18400.00 0.00 0.07396 24400.00 18400.00 0.00 0.07463 24600.00 18400.00 0.00 0.07472 24200.00 18600.00 0.00 0.07537 24400.00 18600.00 0.00 0.07598 24600.00 18600.00 0.00 0.07599 24200.00 18800.00 0.00 0.07671 24400.00 18800.00 0.00 0.07729 24200.00 19000.00 0.00 0.07817 24400.00 19000.00 0.00 0.07876 24600.00 19000.00 0.00 0.07876 24200.00 19200.00 0.00 0.07972 24400.00 19200.00 0.00 0.08031 24200.00 19400.00 0.00 0.08114 24400.00 19400.00 0.00 0.08171 24200.00 19600.00 0.00 0.08258 24400.00 19600.00 0.00 0.08315 24200.00 19800.00 0.00 0.08392 24400.00 19800.00 0.00 0.08449 24200.00 20000.00 0.00 0.08519 24400.00 20000.00 0.00 0.08577 24200.00 20200.00 0.00 0.08666 24400.00 20200.00 0.00 0.08727 24200.00 20400.00 0.00 0.08817 24400.00 20400.00 0.00 0.08878 24200.00 20600.00 0.00 0.08981 24400.00 20600.00 0.00 0.09037 24200.00 20800.00 0.00 0.09148 24400.00 20800.00 0.00 0.09199 24200.00 21000.00 0.00 0.09322 24400.00 21000.00 0.00 0.09368 24200.00 21200.00 0.00 0.09501 24400.00 21200.00 0.00 0.09545 24200.00 21400.00 0.00 0.09695 24400.00 21400.00 0.00 0.09730 24000.00 21600.00 0.00 0.09746 24200.00 21600.00 0.00 0.09889 24400.00 21600.00 0.00 0.09915 24000.00 21800.00 0.00 0.09933 24200.00 21800.00 0.00 0.10067 24400.00 21800.00 0.00 0.10087 24000.00 22000.00 0.00 0.10126 24200.00 22000.00 0.00 0.10255 24400.00 22000.00 0.00 0.10274 24000.00 22200.00 0.00 0.10328 24200.00 22200.00 0.00 0.10457 24400.00 22200.00 0.00 0.10472 24000.00 22400.00 0.00 0.10554 24200.00 22400.00 0.00 0.10679 24400.00 22400.00 0.00 0.10685 24000.00 22600.00 0.00 0.10785 24200.00 22600.00 0.00 0.10902 24000.00 22800.00 0.00 0.11019 24200.00 22800.00 0.00 0.11123 24000.00 23000.00 0.00 0.11240 24200.00 23000.00 0.00 0.11330 24000.00 23200.00 0.00 0.11469 24200.00 23200.00 0.00 0.11539 24000.00 23400.00 0.00 0.11680 24200.00 23400.00 0.00 0.11724 24000.00 23600.00 0.00 0.11845 24200.00 23600.00 0.00 0.11866 23800.00 23800.00 0.00 0.11877 24000.00 23800.00 0.00 0.12029 24000.00 24000.00 0.00 0.12108 23800.00 24200.00 20.00 0.12115 23800.00 24200.00 25.00 0.12130 23800.00 24200.00 30.00 0.12141 23800.00 24200.00 35.00 0.12146 23800.00 24200.00 40.00 0.12146 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23858.89 24125.99 40.75 0.12162 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/280.ctf NX, NY, NZ: 512 512 1 MODE : real