CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/277.mrc Output diagnostic file name Micrographs_0601/277.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/277.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2308.000 7716.000 Mean, RMS : 4460.231 219.1111 TITLE 1: emi2mrc: 277.emi Sun Jan 05 21:50:59 2014 TITLE 2: kV 300, Mag 78000x, Def 24780, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 73.677, Y 595.485, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00151 10200.00 10000.00 0.00 0.00166 10400.00 10000.00 0.00 0.00168 10600.00 10000.00 0.00 0.00169 10800.00 10000.00 0.00 0.00195 11000.00 10000.00 0.00 0.00246 11200.00 10000.00 0.00 0.00309 11400.00 10000.00 0.00 0.00369 11600.00 10000.00 0.00 0.00427 11800.00 10000.00 0.00 0.00472 12000.00 10000.00 0.00 0.00503 12200.00 10000.00 0.00 0.00544 12400.00 10000.00 0.00 0.00593 12600.00 10000.00 0.00 0.00636 12800.00 10000.00 0.00 0.00676 13000.00 10000.00 0.00 0.00710 13200.00 10000.00 0.00 0.00757 13400.00 10000.00 0.00 0.00825 13600.00 10000.00 0.00 0.00903 13800.00 10000.00 0.00 0.00988 14000.00 10000.00 0.00 0.01066 14200.00 10000.00 0.00 0.01138 14400.00 10000.00 0.00 0.01217 14600.00 10000.00 0.00 0.01300 14800.00 10000.00 0.00 0.01395 15000.00 10000.00 0.00 0.01513 15200.00 10000.00 0.00 0.01652 15400.00 10000.00 0.00 0.01785 15600.00 10000.00 0.00 0.01909 15800.00 10000.00 0.00 0.02031 16000.00 10000.00 0.00 0.02160 16200.00 10000.00 0.00 0.02291 16400.00 10000.00 0.00 0.02433 16600.00 10000.00 0.00 0.02576 16800.00 10000.00 0.00 0.02696 17000.00 10000.00 0.00 0.02830 17200.00 10000.00 0.00 0.02975 17400.00 10000.00 0.00 0.03106 17600.00 10000.00 0.00 0.03233 17800.00 10000.00 0.00 0.03367 18000.00 10000.00 0.00 0.03516 18200.00 10000.00 0.00 0.03665 18400.00 10000.00 0.00 0.03816 18600.00 10000.00 0.00 0.03976 18800.00 10000.00 0.00 0.04129 19000.00 10000.00 0.00 0.04288 19200.00 10000.00 0.00 0.04461 19400.00 10000.00 0.00 0.04632 19600.00 10000.00 0.00 0.04804 19800.00 10000.00 0.00 0.04963 20000.00 10000.00 0.00 0.05095 20200.00 10000.00 0.00 0.05210 20400.00 10000.00 0.00 0.05299 20600.00 10000.00 0.00 0.05374 20800.00 10000.00 0.00 0.05419 21000.00 10000.00 0.00 0.05445 21200.00 10000.00 0.00 0.05466 21400.00 10000.00 0.00 0.05476 21600.00 10000.00 0.00 0.05498 21800.00 10000.00 0.00 0.05503 21000.00 10200.00 0.00 0.05523 21200.00 10200.00 0.00 0.05544 21400.00 10200.00 0.00 0.05556 21600.00 10200.00 0.00 0.05574 20800.00 10400.00 0.00 0.05579 21000.00 10400.00 0.00 0.05603 21200.00 10400.00 0.00 0.05621 21400.00 10400.00 0.00 0.05633 21600.00 10400.00 0.00 0.05648 20800.00 10600.00 0.00 0.05658 21000.00 10600.00 0.00 0.05678 21200.00 10600.00 0.00 0.05696 21400.00 10600.00 0.00 0.05710 21600.00 10600.00 0.00 0.05726 21800.00 10600.00 0.00 0.05727 21000.00 10800.00 0.00 0.05747 21200.00 10800.00 0.00 0.05771 21400.00 10800.00 0.00 0.05790 21600.00 10800.00 0.00 0.05807 20800.00 11000.00 0.00 0.05812 21000.00 11000.00 0.00 0.05837 21200.00 11000.00 0.00 0.05867 21400.00 11000.00 0.00 0.05885 21600.00 11000.00 0.00 0.05896 21000.00 11200.00 0.00 0.05914 21200.00 11200.00 0.00 0.05944 21400.00 11200.00 0.00 0.05958 21600.00 11200.00 0.00 0.05965 21000.00 11400.00 0.00 0.06000 21200.00 11400.00 0.00 0.06028 21400.00 11400.00 0.00 0.06041 21600.00 11400.00 0.00 0.06050 20800.00 11600.00 0.00 0.06066 21000.00 11600.00 0.00 0.06101 21200.00 11600.00 0.00 0.06127 21400.00 11600.00 0.00 0.06139 21600.00 11600.00 0.00 0.06147 20800.00 11800.00 0.00 0.06172 21000.00 11800.00 0.00 0.06206 21200.00 11800.00 0.00 0.06230 21400.00 11800.00 0.00 0.06241 21600.00 11800.00 0.00 0.06242 20800.00 12000.00 0.00 0.06285 21000.00 12000.00 0.00 0.06316 21200.00 12000.00 0.00 0.06336 21400.00 12000.00 0.00 0.06342 20800.00 12200.00 0.00 0.06389 21000.00 12200.00 0.00 0.06415 21200.00 12200.00 0.00 0.06431 21400.00 12200.00 0.00 0.06433 20600.00 12400.00 0.00 0.06443 20800.00 12400.00 0.00 0.06492 21000.00 12400.00 0.00 0.06513 21200.00 12400.00 0.00 0.06527 21400.00 12400.00 0.00 0.06528 20600.00 12600.00 0.00 0.06557 20800.00 12600.00 0.00 0.06600 21000.00 12600.00 0.00 0.06614 21200.00 12600.00 0.00 0.06626 20600.00 12800.00 0.00 0.06670 20800.00 12800.00 0.00 0.06705 21000.00 12800.00 0.00 0.06715 21200.00 12800.00 0.00 0.06728 20600.00 13000.00 0.00 0.06782 20800.00 13000.00 0.00 0.06813 21000.00 13000.00 0.00 0.06825 21200.00 13000.00 0.00 0.06842 20400.00 13200.00 0.00 0.06858 20600.00 13200.00 0.00 0.06917 20800.00 13200.00 0.00 0.06947 21000.00 13200.00 0.00 0.06963 21200.00 13200.00 0.00 0.06981 20400.00 13400.00 0.00 0.06984 20600.00 13400.00 0.00 0.07041 20800.00 13400.00 0.00 0.07075 21000.00 13400.00 0.00 0.07096 21200.00 13400.00 0.00 0.07115 20600.00 13600.00 0.00 0.07175 20800.00 13600.00 0.00 0.07214 21000.00 13600.00 0.00 0.07236 21200.00 13600.00 0.00 0.07252 20400.00 13800.00 0.00 0.07267 20600.00 13800.00 0.00 0.07328 20800.00 13800.00 0.00 0.07365 21000.00 13800.00 0.00 0.07382 21200.00 13800.00 0.00 0.07391 20400.00 14000.00 0.00 0.07404 20600.00 14000.00 0.00 0.07462 20800.00 14000.00 0.00 0.07495 21000.00 14000.00 0.00 0.07509 21200.00 14000.00 0.00 0.07513 20400.00 14200.00 0.00 0.07548 20600.00 14200.00 0.00 0.07605 20800.00 14200.00 0.00 0.07640 21000.00 14200.00 0.00 0.07653 21200.00 14200.00 0.00 0.07655 20400.00 14400.00 0.00 0.07697 20600.00 14400.00 0.00 0.07756 20800.00 14400.00 0.00 0.07791 21000.00 14400.00 0.00 0.07801 20400.00 14600.00 0.00 0.07869 20600.00 14600.00 0.00 0.07928 20800.00 14600.00 0.00 0.07961 21000.00 14600.00 0.00 0.07968 20200.00 14800.00 0.00 0.07975 20400.00 14800.00 0.00 0.08060 20600.00 14800.00 0.00 0.08114 20800.00 14800.00 0.00 0.08142 21000.00 14800.00 0.00 0.08144 20200.00 15000.00 0.00 0.08166 20400.00 15000.00 0.00 0.08241 20600.00 15000.00 0.00 0.08290 20800.00 15000.00 0.00 0.08315 20200.00 15200.00 0.00 0.08346 20400.00 15200.00 0.00 0.08416 20600.00 15200.00 0.00 0.08465 20800.00 15200.00 0.00 0.08489 20200.00 15400.00 0.00 0.08522 20400.00 15400.00 0.00 0.08592 20600.00 15400.00 0.00 0.08643 20800.00 15400.00 0.00 0.08663 20200.00 15600.00 0.00 0.08701 20400.00 15600.00 0.00 0.08772 20600.00 15600.00 0.00 0.08823 20800.00 15600.00 0.00 0.08836 20200.00 15800.00 0.00 0.08881 20400.00 15800.00 0.00 0.08952 20600.00 15800.00 0.00 0.08998 20800.00 15800.00 0.00 0.09004 20200.00 16000.00 0.00 0.09061 20400.00 16000.00 0.00 0.09129 20600.00 16000.00 0.00 0.09169 20200.00 16200.00 0.00 0.09230 20400.00 16200.00 0.00 0.09297 20600.00 16200.00 0.00 0.09332 20200.00 16400.00 0.00 0.09423 20400.00 16400.00 0.00 0.09489 20600.00 16400.00 0.00 0.09522 20200.00 16600.00 0.00 0.09616 20400.00 16600.00 0.00 0.09681 20600.00 16600.00 0.00 0.09710 20200.00 16800.00 0.00 0.09808 20400.00 16800.00 0.00 0.09872 20600.00 16800.00 0.00 0.09901 20200.00 17000.00 0.00 0.10019 20400.00 17000.00 0.00 0.10085 20600.00 17000.00 0.00 0.10110 20000.00 17200.00 0.00 0.10118 20200.00 17200.00 0.00 0.10244 20400.00 17200.00 0.00 0.10304 20600.00 17200.00 0.00 0.10322 20000.00 17400.00 0.00 0.10355 20200.00 17400.00 0.00 0.10476 20400.00 17400.00 0.00 0.10528 20600.00 17400.00 0.00 0.10539 20000.00 17600.00 0.00 0.10581 20200.00 17600.00 0.00 0.10697 20400.00 17600.00 0.00 0.10740 20600.00 17600.00 0.00 0.10746 20000.00 17800.00 0.00 0.10820 20200.00 17800.00 0.00 0.10928 20400.00 17800.00 0.00 0.10963 20600.00 17800.00 0.00 0.10963 20000.00 18000.00 0.00 0.11040 20200.00 18000.00 0.00 0.11143 20400.00 18000.00 0.00 0.11172 20000.00 18200.00 0.00 0.11279 20200.00 18200.00 0.00 0.11380 20400.00 18200.00 0.00 0.11405 20000.00 18400.00 0.00 0.11534 20200.00 18400.00 0.00 0.11630 20400.00 18400.00 0.00 0.11642 20000.00 18600.00 0.00 0.11781 20200.00 18600.00 0.00 0.11864 19800.00 18800.00 0.00 0.11907 20000.00 18800.00 0.00 0.12043 20200.00 18800.00 0.00 0.12114 19800.00 19000.00 0.00 0.12162 20000.00 19000.00 0.00 0.12285 20200.00 19000.00 0.00 0.12341 19800.00 19200.00 0.00 0.12438 20000.00 19200.00 0.00 0.12541 20200.00 19200.00 0.00 0.12579 19800.00 19400.00 0.00 0.12698 20000.00 19400.00 0.00 0.12784 20200.00 19400.00 0.00 0.12807 19800.00 19600.00 0.00 0.12994 20000.00 19600.00 0.00 0.13052 20200.00 19600.00 0.00 0.13055 19600.00 19800.00 0.00 0.13091 19800.00 19800.00 0.00 0.13241 20000.00 19800.00 0.00 0.13252 19800.00 20000.00 0.00 0.13299 19800.00 20000.00 5.00 0.13305 19800.00 20000.00 10.00 0.13310 19800.00 20000.00 15.00 0.13314 19800.00 20000.00 20.00 0.13317 19800.00 20000.00 25.00 0.13319 19800.00 20000.00 30.00 0.13320 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19800.00 20087.48 36.02 0.13330 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/277.ctf NX, NY, NZ: 512 512 1 MODE : real