CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/275.mrc Output diagnostic file name Micrographs_0601/275.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/275.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2254.000 8192.000 Mean, RMS : 4461.524 217.8872 TITLE 1: emi2mrc: 275.emi Sun Jan 05 21:49:36 2014 TITLE 2: kV 300, Mag 78000x, Def 24780, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 71.261, Y 598.141, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00735 10800.00 10000.00 0.00 -0.00712 11000.00 10000.00 0.00 -0.00704 11200.00 10000.00 0.00 -0.00700 11800.00 10000.00 0.00 -0.00696 12000.00 10000.00 0.00 -0.00653 12200.00 10000.00 0.00 -0.00613 12400.00 10000.00 0.00 -0.00566 12600.00 10000.00 0.00 -0.00518 12800.00 10000.00 0.00 -0.00475 13000.00 10000.00 0.00 -0.00428 13200.00 10000.00 0.00 -0.00395 13400.00 10000.00 0.00 -0.00368 13600.00 10000.00 0.00 -0.00322 13800.00 10000.00 0.00 -0.00271 14000.00 10000.00 0.00 -0.00219 14200.00 10000.00 0.00 -0.00155 14400.00 10000.00 0.00 -0.00083 14600.00 10000.00 0.00 -0.00032 14800.00 10000.00 0.00 0.00018 15000.00 10000.00 0.00 0.00085 15200.00 10000.00 0.00 0.00152 15400.00 10000.00 0.00 0.00236 15600.00 10000.00 0.00 0.00331 15800.00 10000.00 0.00 0.00418 16000.00 10000.00 0.00 0.00514 16200.00 10000.00 0.00 0.00618 16400.00 10000.00 0.00 0.00712 16600.00 10000.00 0.00 0.00807 16800.00 10000.00 0.00 0.00918 17000.00 10000.00 0.00 0.01024 17200.00 10000.00 0.00 0.01122 17400.00 10000.00 0.00 0.01240 17600.00 10000.00 0.00 0.01370 17800.00 10000.00 0.00 0.01499 18000.00 10000.00 0.00 0.01627 18200.00 10000.00 0.00 0.01753 18400.00 10000.00 0.00 0.01885 18600.00 10000.00 0.00 0.02010 18800.00 10000.00 0.00 0.02133 19000.00 10000.00 0.00 0.02260 19200.00 10000.00 0.00 0.02393 19400.00 10000.00 0.00 0.02545 19600.00 10000.00 0.00 0.02703 19800.00 10000.00 0.00 0.02859 20000.00 10000.00 0.00 0.03026 20200.00 10000.00 0.00 0.03204 20400.00 10000.00 0.00 0.03387 20600.00 10000.00 0.00 0.03581 20800.00 10000.00 0.00 0.03784 21000.00 10000.00 0.00 0.04006 21200.00 10000.00 0.00 0.04231 21400.00 10000.00 0.00 0.04415 21600.00 10000.00 0.00 0.04546 21800.00 10000.00 0.00 0.04628 22000.00 10000.00 0.00 0.04684 22200.00 10000.00 0.00 0.04716 22400.00 10000.00 0.00 0.04727 22000.00 10200.00 0.00 0.04748 22200.00 10200.00 0.00 0.04777 22400.00 10200.00 0.00 0.04783 22000.00 10400.00 0.00 0.04817 22200.00 10400.00 0.00 0.04845 22400.00 10400.00 0.00 0.04850 22000.00 10600.00 0.00 0.04891 22200.00 10600.00 0.00 0.04918 22400.00 10600.00 0.00 0.04919 22000.00 10800.00 0.00 0.04967 22200.00 10800.00 0.00 0.04988 21800.00 11000.00 0.00 0.04996 22000.00 11000.00 0.00 0.05048 22200.00 11000.00 0.00 0.05067 21800.00 11200.00 0.00 0.05083 22000.00 11200.00 0.00 0.05130 22200.00 11200.00 0.00 0.05148 21800.00 11400.00 0.00 0.05164 22000.00 11400.00 0.00 0.05207 22200.00 11400.00 0.00 0.05224 21800.00 11600.00 0.00 0.05232 22000.00 11600.00 0.00 0.05276 22200.00 11600.00 0.00 0.05295 21800.00 11800.00 0.00 0.05307 22000.00 11800.00 0.00 0.05356 22200.00 11800.00 0.00 0.05376 21800.00 12000.00 0.00 0.05395 22000.00 12000.00 0.00 0.05446 22200.00 12000.00 0.00 0.05461 21800.00 12200.00 0.00 0.05510 22000.00 12200.00 0.00 0.05554 22200.00 12200.00 0.00 0.05561 21800.00 12400.00 0.00 0.05608 22000.00 12400.00 0.00 0.05640 21800.00 12600.00 0.00 0.05675 22000.00 12600.00 0.00 0.05700 21800.00 12800.00 0.00 0.05753 22000.00 12800.00 0.00 0.05775 21800.00 13000.00 0.00 0.05822 22000.00 13000.00 0.00 0.05846 22200.00 13000.00 0.00 0.05847 21600.00 13200.00 0.00 0.05854 21800.00 13200.00 0.00 0.05918 22000.00 13200.00 0.00 0.05948 22200.00 13200.00 0.00 0.05953 21600.00 13400.00 0.00 0.05967 21800.00 13400.00 0.00 0.06035 22000.00 13400.00 0.00 0.06072 22200.00 13400.00 0.00 0.06079 21800.00 13600.00 0.00 0.06157 22000.00 13600.00 0.00 0.06198 22200.00 13600.00 0.00 0.06201 21800.00 13800.00 0.00 0.06282 22000.00 13800.00 0.00 0.06318 21600.00 14000.00 0.00 0.06319 21800.00 14000.00 0.00 0.06398 22000.00 14000.00 0.00 0.06424 21600.00 14200.00 0.00 0.06448 21800.00 14200.00 0.00 0.06524 22000.00 14200.00 0.00 0.06549 22200.00 14200.00 0.00 0.06551 21600.00 14400.00 0.00 0.06566 21800.00 14400.00 0.00 0.06639 22000.00 14400.00 0.00 0.06666 22200.00 14400.00 0.00 0.06671 21600.00 14600.00 0.00 0.06688 21800.00 14600.00 0.00 0.06757 22000.00 14600.00 0.00 0.06785 22200.00 14600.00 0.00 0.06786 21600.00 14800.00 0.00 0.06809 21800.00 14800.00 0.00 0.06877 22000.00 14800.00 0.00 0.06906 21600.00 15000.00 0.00 0.06941 21800.00 15000.00 0.00 0.07011 22000.00 15000.00 0.00 0.07040 21600.00 15200.00 0.00 0.07097 21800.00 15200.00 0.00 0.07165 22000.00 15200.00 0.00 0.07192 21600.00 15400.00 0.00 0.07253 21800.00 15400.00 0.00 0.07320 22000.00 15400.00 0.00 0.07348 22200.00 15400.00 0.00 0.07350 21600.00 15600.00 0.00 0.07419 21800.00 15600.00 0.00 0.07486 22000.00 15600.00 0.00 0.07517 22200.00 15600.00 0.00 0.07518 21600.00 15800.00 0.00 0.07577 21800.00 15800.00 0.00 0.07643 22000.00 15800.00 0.00 0.07675 21600.00 16000.00 0.00 0.07734 21800.00 16000.00 0.00 0.07802 22000.00 16000.00 0.00 0.07835 21600.00 16200.00 0.00 0.07892 21800.00 16200.00 0.00 0.07963 22000.00 16200.00 0.00 0.07998 21600.00 16400.00 0.00 0.08051 21800.00 16400.00 0.00 0.08126 22000.00 16400.00 0.00 0.08159 21600.00 16600.00 0.00 0.08216 21800.00 16600.00 0.00 0.08293 22000.00 16600.00 0.00 0.08321 21600.00 16800.00 0.00 0.08388 21800.00 16800.00 0.00 0.08463 22000.00 16800.00 0.00 0.08483 21600.00 17000.00 0.00 0.08564 21800.00 17000.00 0.00 0.08633 22000.00 17000.00 0.00 0.08647 21600.00 17200.00 0.00 0.08734 21800.00 17200.00 0.00 0.08800 22000.00 17200.00 0.00 0.08807 21600.00 17400.00 0.00 0.08905 21800.00 17400.00 0.00 0.08968 21600.00 17600.00 0.00 0.09064 21800.00 17600.00 0.00 0.09122 21600.00 17800.00 0.00 0.09230 21800.00 17800.00 0.00 0.09285 21600.00 18000.00 0.00 0.09406 21800.00 18000.00 0.00 0.09456 21600.00 18200.00 0.00 0.09588 21800.00 18200.00 0.00 0.09628 21600.00 18400.00 0.00 0.09762 21800.00 18400.00 0.00 0.09795 21400.00 18600.00 0.00 0.09804 21600.00 18600.00 0.00 0.09954 21800.00 18600.00 0.00 0.09983 21400.00 18800.00 0.00 0.10019 21600.00 18800.00 0.00 0.10167 21800.00 18800.00 0.00 0.10191 21400.00 19000.00 0.00 0.10208 21600.00 19000.00 0.00 0.10353 21800.00 19000.00 0.00 0.10368 21400.00 19200.00 0.00 0.10419 21600.00 19200.00 0.00 0.10559 21800.00 19200.00 0.00 0.10567 21400.00 19400.00 0.00 0.10647 21600.00 19400.00 0.00 0.10783 21800.00 19400.00 0.00 0.10789 21400.00 19600.00 0.00 0.10893 21600.00 19600.00 0.00 0.11031 21800.00 19600.00 0.00 0.11034 21400.00 19800.00 0.00 0.11157 21600.00 19800.00 0.00 0.11293 21400.00 20000.00 0.00 0.11427 21600.00 20000.00 0.00 0.11551 21400.00 20200.00 0.00 0.11703 21600.00 20200.00 0.00 0.11819 21400.00 20400.00 0.00 0.11995 21600.00 20400.00 0.00 0.12100 21400.00 20600.00 0.00 0.12318 21600.00 20600.00 0.00 0.12400 21400.00 20800.00 0.00 0.12610 21600.00 20800.00 0.00 0.12661 21200.00 21000.00 0.00 0.12726 21400.00 21000.00 0.00 0.12909 21600.00 21000.00 0.00 0.12919 21200.00 21200.00 0.00 0.13024 21400.00 21200.00 0.00 0.13153 21200.00 21400.00 0.00 0.13172 21400.00 21400.00 0.00 0.13260 21200.00 21600.00 30.00 0.13271 21200.00 21600.00 35.00 0.13278 21200.00 21600.00 40.00 0.13280 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21288.75 21519.40 40.00 0.13303 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/275.ctf NX, NY, NZ: 512 512 1 MODE : real