CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/272.mrc Output diagnostic file name Micrographs_0601/272.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/272.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2162.000 8518.000 Mean, RMS : 4458.487 220.6751 TITLE 1: emi2mrc: 272.emi Sun Jan 05 21:47:32 2014 TITLE 2: kV 300, Mag 78000x, Def 24780, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 63.621, Y 598.977, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00168 10200.00 10000.00 0.00 0.00202 10400.00 10000.00 0.00 0.00227 10600.00 10000.00 0.00 0.00246 10800.00 10000.00 0.00 0.00266 11000.00 10000.00 0.00 0.00287 11200.00 10000.00 0.00 0.00317 11400.00 10000.00 0.00 0.00351 11600.00 10000.00 0.00 0.00373 11800.00 10000.00 0.00 0.00385 12000.00 10000.00 0.00 0.00406 12200.00 10000.00 0.00 0.00438 12400.00 10000.00 0.00 0.00467 12600.00 10000.00 0.00 0.00500 12800.00 10000.00 0.00 0.00546 13000.00 10000.00 0.00 0.00591 13200.00 10000.00 0.00 0.00635 13400.00 10000.00 0.00 0.00688 13600.00 10000.00 0.00 0.00735 13800.00 10000.00 0.00 0.00774 14000.00 10000.00 0.00 0.00829 14200.00 10000.00 0.00 0.00897 14400.00 10000.00 0.00 0.00974 14600.00 10000.00 0.00 0.01072 14800.00 10000.00 0.00 0.01175 15000.00 10000.00 0.00 0.01276 15200.00 10000.00 0.00 0.01385 15400.00 10000.00 0.00 0.01496 15600.00 10000.00 0.00 0.01604 15800.00 10000.00 0.00 0.01708 16000.00 10000.00 0.00 0.01835 16200.00 10000.00 0.00 0.01972 16400.00 10000.00 0.00 0.02095 16600.00 10000.00 0.00 0.02234 16800.00 10000.00 0.00 0.02382 17000.00 10000.00 0.00 0.02524 17200.00 10000.00 0.00 0.02658 17400.00 10000.00 0.00 0.02786 17600.00 10000.00 0.00 0.02918 17800.00 10000.00 0.00 0.03050 18000.00 10000.00 0.00 0.03189 18200.00 10000.00 0.00 0.03345 18400.00 10000.00 0.00 0.03511 18600.00 10000.00 0.00 0.03681 18800.00 10000.00 0.00 0.03869 19000.00 10000.00 0.00 0.04049 19200.00 10000.00 0.00 0.04237 19400.00 10000.00 0.00 0.04447 19600.00 10000.00 0.00 0.04645 19800.00 10000.00 0.00 0.04844 20000.00 10000.00 0.00 0.05051 20200.00 10000.00 0.00 0.05271 20400.00 10000.00 0.00 0.05490 20600.00 10000.00 0.00 0.05652 20800.00 10000.00 0.00 0.05737 21000.00 10000.00 0.00 0.05779 21200.00 10000.00 0.00 0.05796 20800.00 10200.00 0.00 0.05813 21000.00 10200.00 0.00 0.05854 21200.00 10200.00 0.00 0.05867 20800.00 10400.00 0.00 0.05884 21000.00 10400.00 0.00 0.05922 21200.00 10400.00 0.00 0.05930 20800.00 10600.00 0.00 0.05946 21000.00 10600.00 0.00 0.05981 21200.00 10600.00 0.00 0.05987 20800.00 10800.00 0.00 0.06011 21000.00 10800.00 0.00 0.06043 21200.00 10800.00 0.00 0.06050 20800.00 11000.00 0.00 0.06094 21000.00 11000.00 0.00 0.06126 21200.00 11000.00 0.00 0.06134 21400.00 11000.00 0.00 0.06134 20800.00 11200.00 0.00 0.06178 21000.00 11200.00 0.00 0.06212 21200.00 11200.00 0.00 0.06221 21400.00 11200.00 0.00 0.06224 20800.00 11400.00 0.00 0.06273 21000.00 11400.00 0.00 0.06306 21200.00 11400.00 0.00 0.06316 21400.00 11400.00 0.00 0.06318 20800.00 11600.00 0.00 0.06367 21000.00 11600.00 0.00 0.06398 21200.00 11600.00 0.00 0.06410 21400.00 11600.00 0.00 0.06412 20800.00 11800.00 0.00 0.06469 21000.00 11800.00 0.00 0.06502 21200.00 11800.00 0.00 0.06516 20800.00 12000.00 0.00 0.06579 21000.00 12000.00 0.00 0.06612 21200.00 12000.00 0.00 0.06625 20800.00 12200.00 0.00 0.06681 21000.00 12200.00 0.00 0.06713 21200.00 12200.00 0.00 0.06724 20800.00 12400.00 0.00 0.06792 21000.00 12400.00 0.00 0.06825 21200.00 12400.00 0.00 0.06838 20800.00 12600.00 0.00 0.06908 21000.00 12600.00 0.00 0.06941 21200.00 12600.00 0.00 0.06955 20800.00 12800.00 0.00 0.07017 21000.00 12800.00 0.00 0.07048 21200.00 12800.00 0.00 0.07062 20800.00 13000.00 0.00 0.07119 21000.00 13000.00 0.00 0.07152 21200.00 13000.00 0.00 0.07166 20800.00 13200.00 0.00 0.07239 21000.00 13200.00 0.00 0.07274 21200.00 13200.00 0.00 0.07287 20800.00 13400.00 0.00 0.07356 21000.00 13400.00 0.00 0.07392 21200.00 13400.00 0.00 0.07404 20800.00 13600.00 0.00 0.07481 21000.00 13600.00 0.00 0.07523 21200.00 13600.00 0.00 0.07535 20800.00 13800.00 0.00 0.07621 21000.00 13800.00 0.00 0.07667 21200.00 13800.00 0.00 0.07673 20800.00 14000.00 0.00 0.07762 21000.00 14000.00 0.00 0.07805 20600.00 14200.00 0.00 0.07813 20800.00 14200.00 0.00 0.07920 21000.00 14200.00 0.00 0.07955 20600.00 14400.00 0.00 0.07962 20800.00 14400.00 0.00 0.08064 21000.00 14400.00 0.00 0.08093 20600.00 14600.00 0.00 0.08113 20800.00 14600.00 0.00 0.08211 21000.00 14600.00 0.00 0.08236 20600.00 14800.00 0.00 0.08258 20800.00 14800.00 0.00 0.08355 21000.00 14800.00 0.00 0.08379 20600.00 15000.00 0.00 0.08407 20800.00 15000.00 0.00 0.08505 21000.00 15000.00 0.00 0.08527 20600.00 15200.00 0.00 0.08566 20800.00 15200.00 0.00 0.08662 21000.00 15200.00 0.00 0.08682 20600.00 15400.00 0.00 0.08728 20800.00 15400.00 0.00 0.08823 21000.00 15400.00 0.00 0.08838 20600.00 15600.00 0.00 0.08916 20800.00 15600.00 0.00 0.09006 21000.00 15600.00 0.00 0.09016 20600.00 15800.00 0.00 0.09094 20800.00 15800.00 0.00 0.09179 21000.00 15800.00 0.00 0.09183 20600.00 16000.00 0.00 0.09259 20800.00 16000.00 0.00 0.09340 20600.00 16200.00 0.00 0.09422 20800.00 16200.00 0.00 0.09502 20600.00 16400.00 0.00 0.09597 20800.00 16400.00 0.00 0.09677 20600.00 16600.00 0.00 0.09778 20800.00 16600.00 0.00 0.09859 20600.00 16800.00 0.00 0.09971 20800.00 16800.00 0.00 0.10053 20600.00 17000.00 0.00 0.10168 20800.00 17000.00 0.00 0.10249 20600.00 17200.00 0.00 0.10365 20800.00 17200.00 0.00 0.10438 20600.00 17400.00 0.00 0.10572 20800.00 17400.00 0.00 0.10636 20600.00 17600.00 0.00 0.10786 20800.00 17600.00 0.00 0.10841 20600.00 17800.00 0.00 0.11014 20800.00 17800.00 0.00 0.11053 20600.00 18000.00 0.00 0.11214 20800.00 18000.00 0.00 0.11238 20400.00 18200.00 0.00 0.11244 20600.00 18200.00 0.00 0.11418 20800.00 18200.00 0.00 0.11434 20400.00 18400.00 0.00 0.11460 20600.00 18400.00 0.00 0.11631 20800.00 18400.00 0.00 0.11642 20400.00 18600.00 0.00 0.11693 20600.00 18600.00 0.00 0.11862 20800.00 18600.00 0.00 0.11865 20400.00 18800.00 0.00 0.11948 20600.00 18800.00 0.00 0.12110 20400.00 19000.00 0.00 0.12186 20600.00 19000.00 0.00 0.12339 20400.00 19200.00 0.00 0.12462 20600.00 19200.00 0.00 0.12603 20400.00 19400.00 0.00 0.12722 20600.00 19400.00 0.00 0.12846 20400.00 19600.00 0.00 0.12982 20600.00 19600.00 0.00 0.13081 20400.00 19800.00 0.00 0.13237 20600.00 19800.00 0.00 0.13311 20400.00 20000.00 0.00 0.13488 20600.00 20000.00 0.00 0.13523 20200.00 20200.00 0.00 0.13542 20400.00 20200.00 0.00 0.13715 20400.00 20400.00 0.00 0.13789 20400.00 20600.00 25.00 0.13796 20200.00 20600.00 30.00 0.13802 20400.00 20600.00 30.00 0.13803 20200.00 20600.00 35.00 0.13819 20200.00 20600.00 40.00 0.13831 20200.00 20600.00 45.00 0.13837 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20277.54 20567.15 46.49 0.13853 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/272.ctf NX, NY, NZ: 512 512 1 MODE : real