CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/270.mrc Output diagnostic file name Micrographs_0601/270.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/270.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2356.000 7922.000 Mean, RMS : 4470.759 220.1261 TITLE 1: emi2mrc: 270.emi Sun Jan 05 20:26:09 2014 TITLE 2: kV 300, Mag 78000x, Def 24780, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 58.431, Y 599.700, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00147 10200.00 10000.00 0.00 -0.00128 10400.00 10000.00 0.00 -0.00113 10600.00 10000.00 0.00 -0.00098 10800.00 10000.00 0.00 -0.00085 11000.00 10000.00 0.00 -0.00071 11200.00 10000.00 0.00 -0.00053 11400.00 10000.00 0.00 -0.00029 11600.00 10000.00 0.00 -0.00004 11800.00 10000.00 0.00 0.00020 12000.00 10000.00 0.00 0.00059 12200.00 10000.00 0.00 0.00089 12400.00 10000.00 0.00 0.00110 12600.00 10000.00 0.00 0.00146 12800.00 10000.00 0.00 0.00177 13000.00 10000.00 0.00 0.00209 13200.00 10000.00 0.00 0.00253 13400.00 10000.00 0.00 0.00305 13600.00 10000.00 0.00 0.00361 13800.00 10000.00 0.00 0.00408 14000.00 10000.00 0.00 0.00454 14200.00 10000.00 0.00 0.00503 14400.00 10000.00 0.00 0.00538 14600.00 10000.00 0.00 0.00582 14800.00 10000.00 0.00 0.00655 15000.00 10000.00 0.00 0.00730 15200.00 10000.00 0.00 0.00801 15400.00 10000.00 0.00 0.00882 15600.00 10000.00 0.00 0.00975 15800.00 10000.00 0.00 0.01070 16000.00 10000.00 0.00 0.01156 16200.00 10000.00 0.00 0.01245 16400.00 10000.00 0.00 0.01341 16600.00 10000.00 0.00 0.01433 16800.00 10000.00 0.00 0.01532 17000.00 10000.00 0.00 0.01641 17200.00 10000.00 0.00 0.01748 17400.00 10000.00 0.00 0.01854 17600.00 10000.00 0.00 0.01965 17800.00 10000.00 0.00 0.02080 18000.00 10000.00 0.00 0.02193 18200.00 10000.00 0.00 0.02300 18400.00 10000.00 0.00 0.02405 18600.00 10000.00 0.00 0.02514 18800.00 10000.00 0.00 0.02631 19000.00 10000.00 0.00 0.02744 19200.00 10000.00 0.00 0.02863 19400.00 10000.00 0.00 0.02996 19600.00 10000.00 0.00 0.03128 19800.00 10000.00 0.00 0.03265 20000.00 10000.00 0.00 0.03401 20200.00 10000.00 0.00 0.03551 20400.00 10000.00 0.00 0.03710 20600.00 10000.00 0.00 0.03863 20800.00 10000.00 0.00 0.04031 21000.00 10000.00 0.00 0.04215 21200.00 10000.00 0.00 0.04417 21400.00 10000.00 0.00 0.04631 21600.00 10000.00 0.00 0.04837 21800.00 10000.00 0.00 0.05034 22000.00 10000.00 0.00 0.05187 22200.00 10000.00 0.00 0.05264 22400.00 10000.00 0.00 0.05307 22600.00 10000.00 0.00 0.05323 22400.00 10200.00 0.00 0.05355 22600.00 10200.00 0.00 0.05374 22200.00 10400.00 0.00 0.05381 22400.00 10400.00 0.00 0.05430 22600.00 10400.00 0.00 0.05450 22200.00 10600.00 0.00 0.05456 22400.00 10600.00 0.00 0.05503 22600.00 10600.00 0.00 0.05521 22200.00 10800.00 0.00 0.05535 22400.00 10800.00 0.00 0.05581 22600.00 10800.00 0.00 0.05596 22200.00 11000.00 0.00 0.05617 22400.00 11000.00 0.00 0.05661 22600.00 11000.00 0.00 0.05674 22200.00 11200.00 0.00 0.05687 22400.00 11200.00 0.00 0.05730 22600.00 11200.00 0.00 0.05741 22200.00 11400.00 0.00 0.05771 22400.00 11400.00 0.00 0.05812 22600.00 11400.00 0.00 0.05819 22200.00 11600.00 0.00 0.05842 22400.00 11600.00 0.00 0.05877 22200.00 11800.00 0.00 0.05906 22400.00 11800.00 0.00 0.05935 22200.00 12000.00 0.00 0.05985 22400.00 12000.00 0.00 0.06011 22200.00 12200.00 0.00 0.06071 22400.00 12200.00 0.00 0.06096 22600.00 12200.00 0.00 0.06097 22200.00 12400.00 0.00 0.06156 22400.00 12400.00 0.00 0.06185 22600.00 12400.00 0.00 0.06190 22200.00 12600.00 0.00 0.06254 22400.00 12600.00 0.00 0.06285 22600.00 12600.00 0.00 0.06291 22200.00 12800.00 0.00 0.06351 22400.00 12800.00 0.00 0.06380 22600.00 12800.00 0.00 0.06382 22200.00 13000.00 0.00 0.06429 22400.00 13000.00 0.00 0.06454 22600.00 13000.00 0.00 0.06455 22000.00 13200.00 0.00 0.06455 22200.00 13200.00 0.00 0.06516 22400.00 13200.00 0.00 0.06540 22600.00 13200.00 0.00 0.06543 22200.00 13400.00 0.00 0.06586 22400.00 13400.00 0.00 0.06613 22600.00 13400.00 0.00 0.06619 22200.00 13600.00 0.00 0.06668 22400.00 13600.00 0.00 0.06702 22600.00 13600.00 0.00 0.06708 22000.00 13800.00 0.00 0.06714 22200.00 13800.00 0.00 0.06781 22400.00 13800.00 0.00 0.06818 22600.00 13800.00 0.00 0.06819 22000.00 14000.00 0.00 0.06830 22200.00 14000.00 0.00 0.06897 22400.00 14000.00 0.00 0.06931 22000.00 14200.00 0.00 0.06947 22200.00 14200.00 0.00 0.07014 22400.00 14200.00 0.00 0.07047 22000.00 14400.00 0.00 0.07055 22200.00 14400.00 0.00 0.07124 22400.00 14400.00 0.00 0.07157 22000.00 14600.00 0.00 0.07173 22200.00 14600.00 0.00 0.07244 22400.00 14600.00 0.00 0.07272 22000.00 14800.00 0.00 0.07284 22200.00 14800.00 0.00 0.07353 22400.00 14800.00 0.00 0.07374 22000.00 15000.00 0.00 0.07395 22200.00 15000.00 0.00 0.07460 22400.00 15000.00 0.00 0.07476 22000.00 15200.00 0.00 0.07508 22200.00 15200.00 0.00 0.07573 22400.00 15200.00 0.00 0.07587 22000.00 15400.00 0.00 0.07634 22200.00 15400.00 0.00 0.07701 22400.00 15400.00 0.00 0.07714 22000.00 15600.00 0.00 0.07775 22200.00 15600.00 0.00 0.07843 22400.00 15600.00 0.00 0.07850 22000.00 15800.00 0.00 0.07908 22200.00 15800.00 0.00 0.07973 22400.00 15800.00 0.00 0.07975 22000.00 16000.00 0.00 0.08063 22200.00 16000.00 0.00 0.08125 22000.00 16200.00 0.00 0.08216 22200.00 16200.00 0.00 0.08276 22000.00 16400.00 0.00 0.08367 22200.00 16400.00 0.00 0.08430 22000.00 16600.00 0.00 0.08525 22200.00 16600.00 0.00 0.08588 22000.00 16800.00 0.00 0.08691 22200.00 16800.00 0.00 0.08747 22000.00 17000.00 0.00 0.08849 22200.00 17000.00 0.00 0.08895 22000.00 17200.00 0.00 0.08995 22200.00 17200.00 0.00 0.09037 22000.00 17400.00 0.00 0.09165 22200.00 17400.00 0.00 0.09201 22000.00 17600.00 0.00 0.09319 22200.00 17600.00 0.00 0.09354 22000.00 17800.00 0.00 0.09488 22200.00 17800.00 0.00 0.09524 22000.00 18000.00 0.00 0.09679 22200.00 18000.00 0.00 0.09712 22000.00 18200.00 0.00 0.09871 22200.00 18200.00 0.00 0.09896 21800.00 18400.00 0.00 0.09896 22000.00 18400.00 0.00 0.10050 22200.00 18400.00 0.00 0.10067 21800.00 18600.00 0.00 0.10075 22000.00 18600.00 0.00 0.10227 22200.00 18600.00 0.00 0.10239 21800.00 18800.00 0.00 0.10271 22000.00 18800.00 0.00 0.10422 22200.00 18800.00 0.00 0.10429 21800.00 19000.00 0.00 0.10454 22000.00 19000.00 0.00 0.10603 21800.00 19200.00 0.00 0.10643 22000.00 19200.00 0.00 0.10788 21800.00 19400.00 0.00 0.10837 22000.00 19400.00 0.00 0.10978 21800.00 19600.00 0.00 0.11053 22000.00 19600.00 0.00 0.11183 21800.00 19800.00 0.00 0.11262 22000.00 19800.00 0.00 0.11382 21800.00 20000.00 0.00 0.11466 22000.00 20000.00 0.00 0.11579 21800.00 20200.00 0.00 0.11692 22000.00 20200.00 0.00 0.11802 21800.00 20400.00 0.00 0.11920 22000.00 20400.00 0.00 0.12020 21800.00 20600.00 0.00 0.12159 22000.00 20600.00 0.00 0.12243 21800.00 20800.00 0.00 0.12383 22000.00 20800.00 0.00 0.12451 21800.00 21000.00 0.00 0.12616 22000.00 21000.00 0.00 0.12659 21800.00 21200.00 0.00 0.12820 22000.00 21200.00 0.00 0.12832 21800.00 21400.00 0.00 0.13000 21600.00 21600.00 0.00 0.13015 21800.00 21600.00 0.00 0.13150 21800.00 21800.00 0.00 0.13214 21600.00 22000.00 25.00 0.13224 21600.00 22000.00 30.00 0.13234 21600.00 22000.00 35.00 0.13238 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21685.20 21928.80 35.38 0.13260 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/270.ctf NX, NY, NZ: 512 512 1 MODE : real