CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/269.mrc Output diagnostic file name Micrographs_0601/269.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/269.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2596.000 9098.000 Mean, RMS : 4475.310 222.7215 TITLE 1: emi2mrc: 269.emi Sun Jan 05 20:25:28 2014 TITLE 2: kV 300, Mag 78000x, Def 24780, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 55.811, Y 599.959, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00015 10200.00 10000.00 0.00 -0.00012 11000.00 10000.00 0.00 -0.00001 11200.00 10000.00 0.00 0.00017 11400.00 10000.00 0.00 0.00030 11600.00 10000.00 0.00 0.00042 11800.00 10000.00 0.00 0.00058 12000.00 10000.00 0.00 0.00079 12200.00 10000.00 0.00 0.00100 12400.00 10000.00 0.00 0.00123 12600.00 10000.00 0.00 0.00144 12800.00 10000.00 0.00 0.00171 13000.00 10000.00 0.00 0.00205 13200.00 10000.00 0.00 0.00232 13400.00 10000.00 0.00 0.00270 13600.00 10000.00 0.00 0.00325 13800.00 10000.00 0.00 0.00390 14000.00 10000.00 0.00 0.00455 14200.00 10000.00 0.00 0.00514 14400.00 10000.00 0.00 0.00576 14600.00 10000.00 0.00 0.00637 14800.00 10000.00 0.00 0.00699 15000.00 10000.00 0.00 0.00769 15200.00 10000.00 0.00 0.00843 15400.00 10000.00 0.00 0.00914 15600.00 10000.00 0.00 0.00996 15800.00 10000.00 0.00 0.01091 16000.00 10000.00 0.00 0.01179 16200.00 10000.00 0.00 0.01278 16400.00 10000.00 0.00 0.01374 16600.00 10000.00 0.00 0.01449 16800.00 10000.00 0.00 0.01542 17000.00 10000.00 0.00 0.01630 17200.00 10000.00 0.00 0.01714 17400.00 10000.00 0.00 0.01823 17600.00 10000.00 0.00 0.01931 17800.00 10000.00 0.00 0.02045 18000.00 10000.00 0.00 0.02165 18200.00 10000.00 0.00 0.02293 18400.00 10000.00 0.00 0.02436 18600.00 10000.00 0.00 0.02568 18800.00 10000.00 0.00 0.02692 19000.00 10000.00 0.00 0.02821 19200.00 10000.00 0.00 0.02957 19400.00 10000.00 0.00 0.03091 19600.00 10000.00 0.00 0.03239 19800.00 10000.00 0.00 0.03394 20000.00 10000.00 0.00 0.03543 20200.00 10000.00 0.00 0.03705 20400.00 10000.00 0.00 0.03863 20600.00 10000.00 0.00 0.04029 20800.00 10000.00 0.00 0.04213 21000.00 10000.00 0.00 0.04410 21200.00 10000.00 0.00 0.04617 21400.00 10000.00 0.00 0.04835 21600.00 10000.00 0.00 0.05038 21800.00 10000.00 0.00 0.05178 22000.00 10000.00 0.00 0.05259 22200.00 10000.00 0.00 0.05287 22400.00 10000.00 0.00 0.05301 22600.00 10000.00 0.00 0.05306 22000.00 10200.00 0.00 0.05325 22200.00 10200.00 0.00 0.05352 22400.00 10200.00 0.00 0.05368 22600.00 10200.00 0.00 0.05376 22000.00 10400.00 0.00 0.05390 22200.00 10400.00 0.00 0.05422 22400.00 10400.00 0.00 0.05445 22600.00 10400.00 0.00 0.05454 22200.00 10600.00 0.00 0.05491 22400.00 10600.00 0.00 0.05514 22600.00 10600.00 0.00 0.05517 22200.00 10800.00 0.00 0.05548 22400.00 10800.00 0.00 0.05567 22000.00 11000.00 0.00 0.05581 22200.00 11000.00 0.00 0.05619 22400.00 11000.00 0.00 0.05636 22000.00 11200.00 0.00 0.05655 22200.00 11200.00 0.00 0.05693 22400.00 11200.00 0.00 0.05706 22000.00 11400.00 0.00 0.05724 22200.00 11400.00 0.00 0.05758 22400.00 11400.00 0.00 0.05766 22000.00 11600.00 0.00 0.05786 22200.00 11600.00 0.00 0.05819 22400.00 11600.00 0.00 0.05826 22000.00 11800.00 0.00 0.05864 22200.00 11800.00 0.00 0.05894 22400.00 11800.00 0.00 0.05899 22000.00 12000.00 0.00 0.05941 22200.00 12000.00 0.00 0.05966 22400.00 12000.00 0.00 0.05968 22000.00 12200.00 0.00 0.06007 22200.00 12200.00 0.00 0.06032 22400.00 12200.00 0.00 0.06033 22000.00 12400.00 0.00 0.06096 22200.00 12400.00 0.00 0.06121 22400.00 12400.00 0.00 0.06125 22000.00 12600.00 0.00 0.06196 22200.00 12600.00 0.00 0.06223 22400.00 12600.00 0.00 0.06229 22000.00 12800.00 0.00 0.06295 22200.00 12800.00 0.00 0.06326 22400.00 12800.00 0.00 0.06337 22000.00 13000.00 0.00 0.06398 22200.00 13000.00 0.00 0.06432 22400.00 13000.00 0.00 0.06440 22000.00 13200.00 0.00 0.06498 22200.00 13200.00 0.00 0.06528 22400.00 13200.00 0.00 0.06530 22000.00 13400.00 0.00 0.06581 22200.00 13400.00 0.00 0.06608 22000.00 13600.00 0.00 0.06673 22200.00 13600.00 0.00 0.06700 21800.00 13800.00 0.00 0.06700 22000.00 13800.00 0.00 0.06785 22200.00 13800.00 0.00 0.06811 21800.00 14000.00 0.00 0.06817 22000.00 14000.00 0.00 0.06901 22200.00 14000.00 0.00 0.06924 21800.00 14200.00 0.00 0.06925 22000.00 14200.00 0.00 0.07009 22200.00 14200.00 0.00 0.07028 22000.00 14400.00 0.00 0.07110 22200.00 14400.00 0.00 0.07127 21800.00 14600.00 0.00 0.07154 22000.00 14600.00 0.00 0.07240 22200.00 14600.00 0.00 0.07254 21800.00 14800.00 0.00 0.07293 22000.00 14800.00 0.00 0.07376 22200.00 14800.00 0.00 0.07385 21800.00 15000.00 0.00 0.07426 22000.00 15000.00 0.00 0.07505 22200.00 15000.00 0.00 0.07509 21800.00 15200.00 0.00 0.07560 22000.00 15200.00 0.00 0.07636 22200.00 15200.00 0.00 0.07637 21800.00 15400.00 0.00 0.07698 22000.00 15400.00 0.00 0.07772 21800.00 15600.00 0.00 0.07837 22000.00 15600.00 0.00 0.07911 21800.00 15800.00 0.00 0.07976 22000.00 15800.00 0.00 0.08050 21800.00 16000.00 0.00 0.08126 22000.00 16000.00 0.00 0.08196 21800.00 16200.00 0.00 0.08289 22000.00 16200.00 0.00 0.08354 21800.00 16400.00 0.00 0.08468 22000.00 16400.00 0.00 0.08524 21800.00 16600.00 0.00 0.08628 22000.00 16600.00 0.00 0.08676 21800.00 16800.00 0.00 0.08785 22000.00 16800.00 0.00 0.08829 21800.00 17000.00 0.00 0.08941 22000.00 17000.00 0.00 0.08981 21800.00 17200.00 0.00 0.09098 22000.00 17200.00 0.00 0.09135 21600.00 17400.00 0.00 0.09135 21800.00 17400.00 0.00 0.09274 22000.00 17400.00 0.00 0.09310 21800.00 17600.00 0.00 0.09428 22000.00 17600.00 0.00 0.09465 21800.00 17800.00 0.00 0.09604 22000.00 17800.00 0.00 0.09646 21600.00 18000.00 0.00 0.09659 21800.00 18000.00 0.00 0.09804 22000.00 18000.00 0.00 0.09847 21600.00 18200.00 0.00 0.09862 21800.00 18200.00 0.00 0.10008 22000.00 18200.00 0.00 0.10046 21600.00 18400.00 0.00 0.10069 21800.00 18400.00 0.00 0.10214 22000.00 18400.00 0.00 0.10245 21600.00 18600.00 0.00 0.10283 21800.00 18600.00 0.00 0.10423 22000.00 18600.00 0.00 0.10447 21600.00 18800.00 0.00 0.10498 21800.00 18800.00 0.00 0.10632 22000.00 18800.00 0.00 0.10648 21600.00 19000.00 0.00 0.10707 21800.00 19000.00 0.00 0.10838 22000.00 19000.00 0.00 0.10851 21600.00 19200.00 0.00 0.10949 21800.00 19200.00 0.00 0.11079 22000.00 19200.00 0.00 0.11085 21600.00 19400.00 0.00 0.11180 21800.00 19400.00 0.00 0.11302 21600.00 19600.00 0.00 0.11414 21800.00 19600.00 0.00 0.11527 21600.00 19800.00 0.00 0.11656 21800.00 19800.00 0.00 0.11763 21600.00 20000.00 0.00 0.11922 21800.00 20000.00 0.00 0.12026 21600.00 20200.00 0.00 0.12219 21800.00 20200.00 0.00 0.12312 21600.00 20400.00 0.00 0.12505 21800.00 20400.00 0.00 0.12581 21600.00 20600.00 0.00 0.12804 21800.00 20600.00 0.00 0.12859 21600.00 20800.00 0.00 0.13095 21800.00 20800.00 0.00 0.13125 21400.00 21000.00 0.00 0.13183 21600.00 21000.00 0.00 0.13411 21400.00 21200.00 0.00 0.13497 21600.00 21200.00 0.00 0.13697 21400.00 21400.00 0.00 0.13790 21600.00 21400.00 0.00 0.13945 21400.00 21600.00 0.00 0.13970 21600.00 21600.00 0.00 0.14042 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21496.28 21654.99 39.58 0.14088 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/269.ctf NX, NY, NZ: 512 512 1 MODE : real