CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/264.mrc Output diagnostic file name Micrographs_0601/264.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/264.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2034.000 7960.000 Mean, RMS : 4455.324 217.4282 TITLE 1: emi2mrc: 264.emi Sun Jan 05 20:18:35 2014 TITLE 2: kV 300, Mag 78000x, Def 24940, Spot 4, Int 55.08%, Obj 88.09%, Dif 73.67% TITLE 3: X 58.280, Y 602.667, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00825 10200.00 10000.00 0.00 -0.00813 10400.00 10000.00 0.00 -0.00796 10600.00 10000.00 0.00 -0.00787 10800.00 10000.00 0.00 -0.00767 11000.00 10000.00 0.00 -0.00747 11200.00 10000.00 0.00 -0.00719 11400.00 10000.00 0.00 -0.00685 11600.00 10000.00 0.00 -0.00661 11800.00 10000.00 0.00 -0.00640 12000.00 10000.00 0.00 -0.00605 12200.00 10000.00 0.00 -0.00568 12400.00 10000.00 0.00 -0.00533 12600.00 10000.00 0.00 -0.00483 12800.00 10000.00 0.00 -0.00439 13000.00 10000.00 0.00 -0.00393 13200.00 10000.00 0.00 -0.00346 13400.00 10000.00 0.00 -0.00302 13600.00 10000.00 0.00 -0.00237 13800.00 10000.00 0.00 -0.00176 14000.00 10000.00 0.00 -0.00109 14200.00 10000.00 0.00 -0.00019 14400.00 10000.00 0.00 0.00065 14600.00 10000.00 0.00 0.00154 14800.00 10000.00 0.00 0.00249 15000.00 10000.00 0.00 0.00339 15200.00 10000.00 0.00 0.00432 15400.00 10000.00 0.00 0.00528 15600.00 10000.00 0.00 0.00635 15800.00 10000.00 0.00 0.00742 16000.00 10000.00 0.00 0.00838 16200.00 10000.00 0.00 0.00956 16400.00 10000.00 0.00 0.01091 16600.00 10000.00 0.00 0.01226 16800.00 10000.00 0.00 0.01369 17000.00 10000.00 0.00 0.01507 17200.00 10000.00 0.00 0.01627 17400.00 10000.00 0.00 0.01732 17600.00 10000.00 0.00 0.01836 17800.00 10000.00 0.00 0.01947 18000.00 10000.00 0.00 0.02056 18200.00 10000.00 0.00 0.02164 18400.00 10000.00 0.00 0.02289 18600.00 10000.00 0.00 0.02423 18800.00 10000.00 0.00 0.02556 19000.00 10000.00 0.00 0.02692 19200.00 10000.00 0.00 0.02831 19400.00 10000.00 0.00 0.02982 19600.00 10000.00 0.00 0.03149 19800.00 10000.00 0.00 0.03300 20000.00 10000.00 0.00 0.03452 20200.00 10000.00 0.00 0.03621 20400.00 10000.00 0.00 0.03801 20600.00 10000.00 0.00 0.04000 20800.00 10000.00 0.00 0.04208 21000.00 10000.00 0.00 0.04398 21200.00 10000.00 0.00 0.04550 21400.00 10000.00 0.00 0.04672 21600.00 10000.00 0.00 0.04749 21800.00 10000.00 0.00 0.04783 22000.00 10000.00 0.00 0.04803 21600.00 10200.00 0.00 0.04807 21800.00 10200.00 0.00 0.04838 22000.00 10200.00 0.00 0.04858 21600.00 10400.00 0.00 0.04877 21800.00 10400.00 0.00 0.04906 22000.00 10400.00 0.00 0.04928 21600.00 10600.00 0.00 0.04955 21800.00 10600.00 0.00 0.04982 22000.00 10600.00 0.00 0.05003 21600.00 10800.00 0.00 0.05029 21800.00 10800.00 0.00 0.05054 22000.00 10800.00 0.00 0.05074 21600.00 11000.00 0.00 0.05111 21800.00 11000.00 0.00 0.05135 22000.00 11000.00 0.00 0.05154 21600.00 11200.00 0.00 0.05185 21800.00 11200.00 0.00 0.05208 22000.00 11200.00 0.00 0.05225 21600.00 11400.00 0.00 0.05265 21800.00 11400.00 0.00 0.05288 22000.00 11400.00 0.00 0.05303 21600.00 11600.00 0.00 0.05342 21800.00 11600.00 0.00 0.05363 22000.00 11600.00 0.00 0.05371 21600.00 11800.00 0.00 0.05403 21800.00 11800.00 0.00 0.05418 21600.00 12000.00 0.00 0.05452 21800.00 12000.00 0.00 0.05462 21400.00 12200.00 0.00 0.05474 21600.00 12200.00 0.00 0.05515 21800.00 12200.00 0.00 0.05526 22000.00 12200.00 0.00 0.05526 21400.00 12400.00 0.00 0.05561 21600.00 12400.00 0.00 0.05600 21800.00 12400.00 0.00 0.05612 22000.00 12400.00 0.00 0.05612 21400.00 12600.00 0.00 0.05658 21600.00 12600.00 0.00 0.05695 21800.00 12600.00 0.00 0.05705 21400.00 12800.00 0.00 0.05767 21600.00 12800.00 0.00 0.05802 21800.00 12800.00 0.00 0.05811 21400.00 13000.00 0.00 0.05872 21600.00 13000.00 0.00 0.05907 21800.00 13000.00 0.00 0.05914 21400.00 13200.00 0.00 0.05989 21600.00 13200.00 0.00 0.06022 21800.00 13200.00 0.00 0.06025 21400.00 13400.00 0.00 0.06109 21600.00 13400.00 0.00 0.06139 21800.00 13400.00 0.00 0.06140 21400.00 13600.00 0.00 0.06218 21600.00 13600.00 0.00 0.06244 21800.00 13600.00 0.00 0.06245 21400.00 13800.00 0.00 0.06331 21600.00 13800.00 0.00 0.06354 21800.00 13800.00 0.00 0.06355 21200.00 14000.00 0.00 0.06368 21400.00 14000.00 0.00 0.06453 21600.00 14000.00 0.00 0.06473 21800.00 14000.00 0.00 0.06473 21200.00 14200.00 0.00 0.06481 21400.00 14200.00 0.00 0.06562 21600.00 14200.00 0.00 0.06582 21800.00 14200.00 0.00 0.06585 21400.00 14400.00 0.00 0.06665 21600.00 14400.00 0.00 0.06689 21800.00 14400.00 0.00 0.06698 22000.00 14400.00 0.00 0.06699 21200.00 14600.00 0.00 0.06713 21400.00 14600.00 0.00 0.06800 21600.00 14600.00 0.00 0.06830 21800.00 14600.00 0.00 0.06845 22000.00 14600.00 0.00 0.06848 21200.00 14800.00 0.00 0.06860 21400.00 14800.00 0.00 0.06951 21600.00 14800.00 0.00 0.06984 21800.00 14800.00 0.00 0.06998 21200.00 15000.00 0.00 0.07028 21400.00 15000.00 0.00 0.07118 21600.00 15000.00 0.00 0.07150 21800.00 15000.00 0.00 0.07159 21200.00 15200.00 0.00 0.07184 21400.00 15200.00 0.00 0.07272 21600.00 15200.00 0.00 0.07301 21800.00 15200.00 0.00 0.07304 21200.00 15400.00 0.00 0.07321 21400.00 15400.00 0.00 0.07408 21600.00 15400.00 0.00 0.07432 21200.00 15600.00 0.00 0.07471 21400.00 15600.00 0.00 0.07552 21600.00 15600.00 0.00 0.07569 21200.00 15800.00 0.00 0.07618 21400.00 15800.00 0.00 0.07692 21600.00 15800.00 0.00 0.07705 21200.00 16000.00 0.00 0.07764 21400.00 16000.00 0.00 0.07838 21600.00 16000.00 0.00 0.07853 21200.00 16200.00 0.00 0.07912 21400.00 16200.00 0.00 0.07987 21600.00 16200.00 0.00 0.08003 21200.00 16400.00 0.00 0.08082 21400.00 16400.00 0.00 0.08153 21600.00 16400.00 0.00 0.08165 21200.00 16600.00 0.00 0.08243 21400.00 16600.00 0.00 0.08314 21600.00 16600.00 0.00 0.08323 21200.00 16800.00 0.00 0.08407 21400.00 16800.00 0.00 0.08477 21600.00 16800.00 0.00 0.08482 21200.00 17000.00 0.00 0.08592 21400.00 17000.00 0.00 0.08656 21600.00 17000.00 0.00 0.08657 21200.00 17200.00 0.00 0.08765 21400.00 17200.00 0.00 0.08824 21600.00 17200.00 0.00 0.08825 21200.00 17400.00 0.00 0.08942 21400.00 17400.00 0.00 0.08999 21600.00 17400.00 0.00 0.08999 21200.00 17600.00 0.00 0.09112 21400.00 17600.00 0.00 0.09165 21200.00 17800.00 0.00 0.09273 21400.00 17800.00 0.00 0.09328 21200.00 18000.00 0.00 0.09448 21400.00 18000.00 0.00 0.09500 21000.00 18200.00 0.00 0.09512 21200.00 18200.00 0.00 0.09634 21400.00 18200.00 0.00 0.09679 21000.00 18400.00 0.00 0.09686 21200.00 18400.00 0.00 0.09806 21400.00 18400.00 0.00 0.09849 21000.00 18600.00 0.00 0.09869 21200.00 18600.00 0.00 0.09991 21400.00 18600.00 0.00 0.10033 21000.00 18800.00 0.00 0.10063 21200.00 18800.00 0.00 0.10187 21400.00 18800.00 0.00 0.10220 21000.00 19000.00 0.00 0.10244 21200.00 19000.00 0.00 0.10365 21400.00 19000.00 0.00 0.10388 21000.00 19200.00 0.00 0.10457 21200.00 19200.00 0.00 0.10570 21400.00 19200.00 0.00 0.10578 21000.00 19400.00 0.00 0.10665 21200.00 19400.00 0.00 0.10764 21000.00 19600.00 0.00 0.10879 21200.00 19600.00 0.00 0.10966 21000.00 19800.00 0.00 0.11086 21200.00 19800.00 0.00 0.11169 21000.00 20000.00 0.00 0.11301 21200.00 20000.00 0.00 0.11384 20800.00 20200.00 0.00 0.11387 21000.00 20200.00 0.00 0.11529 21200.00 20200.00 0.00 0.11604 21000.00 20400.00 0.00 0.11734 21200.00 20400.00 0.00 0.11797 20800.00 20600.00 0.00 0.11834 21000.00 20600.00 0.00 0.11948 21200.00 20600.00 0.00 0.11999 20800.00 20800.00 0.00 0.12009 21000.00 20800.00 0.00 0.12121 21200.00 20800.00 0.00 0.12158 20800.00 21000.00 0.00 0.12159 21000.00 21000.00 0.00 0.12265 21000.00 21200.00 5.00 0.12278 21000.00 21200.00 10.00 0.12292 21000.00 21200.00 15.00 0.12304 20800.00 21200.00 20.00 0.12305 21000.00 21200.00 20.00 0.12315 20800.00 21200.00 25.00 0.12324 21000.00 21200.00 25.00 0.12325 20800.00 21200.00 30.00 0.12339 20800.00 21200.00 35.00 0.12348 20800.00 21200.00 40.00 0.12350 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20800.00 21205.51 40.00 0.12350 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/264.ctf NX, NY, NZ: 512 512 1 MODE : real