CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/263.mrc Output diagnostic file name Micrographs_0601/263.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/263.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2590.000 8058.000 Mean, RMS : 4446.572 219.5577 TITLE 1: emi2mrc: 263.emi Sun Jan 05 20:17:56 2014 TITLE 2: kV 300, Mag 78000x, Def 24940, Spot 4, Int 55.08%, Obj 88.09%, Dif 73.67% TITLE 3: X 60.971, Y 602.344, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00423 10200.00 10000.00 0.00 -0.00417 10400.00 10000.00 0.00 -0.00396 10600.00 10000.00 0.00 -0.00382 10800.00 10000.00 0.00 -0.00376 11000.00 10000.00 0.00 -0.00370 11200.00 10000.00 0.00 -0.00356 11400.00 10000.00 0.00 -0.00333 11600.00 10000.00 0.00 -0.00321 11800.00 10000.00 0.00 -0.00306 12000.00 10000.00 0.00 -0.00286 12200.00 10000.00 0.00 -0.00258 12400.00 10000.00 0.00 -0.00212 12600.00 10000.00 0.00 -0.00169 12800.00 10000.00 0.00 -0.00128 13000.00 10000.00 0.00 -0.00085 13200.00 10000.00 0.00 -0.00044 13400.00 10000.00 0.00 0.00012 13600.00 10000.00 0.00 0.00057 13800.00 10000.00 0.00 0.00086 14000.00 10000.00 0.00 0.00121 14200.00 10000.00 0.00 0.00167 14400.00 10000.00 0.00 0.00222 14600.00 10000.00 0.00 0.00272 14800.00 10000.00 0.00 0.00325 15000.00 10000.00 0.00 0.00377 15200.00 10000.00 0.00 0.00433 15400.00 10000.00 0.00 0.00496 15600.00 10000.00 0.00 0.00563 15800.00 10000.00 0.00 0.00631 16000.00 10000.00 0.00 0.00695 16200.00 10000.00 0.00 0.00772 16400.00 10000.00 0.00 0.00865 16600.00 10000.00 0.00 0.00961 16800.00 10000.00 0.00 0.01058 17000.00 10000.00 0.00 0.01158 17200.00 10000.00 0.00 0.01265 17400.00 10000.00 0.00 0.01366 17600.00 10000.00 0.00 0.01471 17800.00 10000.00 0.00 0.01600 18000.00 10000.00 0.00 0.01730 18200.00 10000.00 0.00 0.01853 18400.00 10000.00 0.00 0.01979 18600.00 10000.00 0.00 0.02103 18800.00 10000.00 0.00 0.02226 19000.00 10000.00 0.00 0.02350 19200.00 10000.00 0.00 0.02475 19400.00 10000.00 0.00 0.02597 19600.00 10000.00 0.00 0.02714 19800.00 10000.00 0.00 0.02832 20000.00 10000.00 0.00 0.02950 20200.00 10000.00 0.00 0.03084 20400.00 10000.00 0.00 0.03229 20600.00 10000.00 0.00 0.03385 20800.00 10000.00 0.00 0.03549 21000.00 10000.00 0.00 0.03718 21200.00 10000.00 0.00 0.03892 21400.00 10000.00 0.00 0.04072 21600.00 10000.00 0.00 0.04263 21800.00 10000.00 0.00 0.04455 22000.00 10000.00 0.00 0.04657 22200.00 10000.00 0.00 0.04849 22400.00 10000.00 0.00 0.04987 22600.00 10000.00 0.00 0.05066 22800.00 10000.00 0.00 0.05094 23000.00 10000.00 0.00 0.05110 23200.00 10000.00 0.00 0.05114 22600.00 10200.00 0.00 0.05115 22800.00 10200.00 0.00 0.05142 23000.00 10200.00 0.00 0.05161 23200.00 10200.00 0.00 0.05163 22800.00 10400.00 0.00 0.05191 23000.00 10400.00 0.00 0.05215 23200.00 10400.00 0.00 0.05216 22800.00 10600.00 0.00 0.05249 23000.00 10600.00 0.00 0.05275 22800.00 10800.00 0.00 0.05319 23000.00 10800.00 0.00 0.05342 22600.00 11000.00 0.00 0.05356 22800.00 11000.00 0.00 0.05401 23000.00 11000.00 0.00 0.05417 22600.00 11200.00 0.00 0.05428 22800.00 11200.00 0.00 0.05465 23000.00 11200.00 0.00 0.05475 22600.00 11400.00 0.00 0.05488 22800.00 11400.00 0.00 0.05521 23000.00 11400.00 0.00 0.05531 22600.00 11600.00 0.00 0.05554 22800.00 11600.00 0.00 0.05587 23000.00 11600.00 0.00 0.05598 22600.00 11800.00 0.00 0.05630 22800.00 11800.00 0.00 0.05661 23000.00 11800.00 0.00 0.05669 22600.00 12000.00 0.00 0.05703 22800.00 12000.00 0.00 0.05731 23000.00 12000.00 0.00 0.05737 22600.00 12200.00 0.00 0.05788 22800.00 12200.00 0.00 0.05814 23000.00 12200.00 0.00 0.05820 22600.00 12400.00 0.00 0.05875 22800.00 12400.00 0.00 0.05898 23000.00 12400.00 0.00 0.05903 22600.00 12600.00 0.00 0.05940 22800.00 12600.00 0.00 0.05962 23000.00 12600.00 0.00 0.05967 22600.00 12800.00 0.00 0.06023 22800.00 12800.00 0.00 0.06043 23000.00 12800.00 0.00 0.06050 22600.00 13000.00 0.00 0.06115 22800.00 13000.00 0.00 0.06136 23000.00 13000.00 0.00 0.06145 22600.00 13200.00 0.00 0.06213 22800.00 13200.00 0.00 0.06236 23000.00 13200.00 0.00 0.06249 22600.00 13400.00 0.00 0.06317 22800.00 13400.00 0.00 0.06342 23000.00 13400.00 0.00 0.06356 22600.00 13600.00 0.00 0.06413 22800.00 13600.00 0.00 0.06439 23000.00 13600.00 0.00 0.06453 22600.00 13800.00 0.00 0.06503 22800.00 13800.00 0.00 0.06530 23000.00 13800.00 0.00 0.06540 22600.00 14000.00 0.00 0.06593 22800.00 14000.00 0.00 0.06619 23000.00 14000.00 0.00 0.06623 22600.00 14200.00 0.00 0.06686 22800.00 14200.00 0.00 0.06708 22400.00 14400.00 0.00 0.06709 22600.00 14400.00 0.00 0.06778 22800.00 14400.00 0.00 0.06797 22400.00 14600.00 0.00 0.06820 22600.00 14600.00 0.00 0.06889 22800.00 14600.00 0.00 0.06908 22400.00 14800.00 0.00 0.06930 22600.00 14800.00 0.00 0.07000 22800.00 14800.00 0.00 0.07015 22400.00 15000.00 0.00 0.07046 22600.00 15000.00 0.00 0.07113 22800.00 15000.00 0.00 0.07122 22400.00 15200.00 0.00 0.07167 22600.00 15200.00 0.00 0.07228 22800.00 15200.00 0.00 0.07231 22400.00 15400.00 0.00 0.07281 22600.00 15400.00 0.00 0.07337 22400.00 15600.00 0.00 0.07395 22600.00 15600.00 0.00 0.07447 22400.00 15800.00 0.00 0.07507 22600.00 15800.00 0.00 0.07559 22400.00 16000.00 0.00 0.07631 22600.00 16000.00 0.00 0.07684 22400.00 16200.00 0.00 0.07759 22600.00 16200.00 0.00 0.07812 22400.00 16400.00 0.00 0.07889 22600.00 16400.00 0.00 0.07939 22400.00 16600.00 0.00 0.08017 22600.00 16600.00 0.00 0.08064 22400.00 16800.00 0.00 0.08159 22600.00 16800.00 0.00 0.08202 22400.00 17000.00 0.00 0.08299 22600.00 17000.00 0.00 0.08335 22400.00 17200.00 0.00 0.08439 22600.00 17200.00 0.00 0.08473 22200.00 17400.00 0.00 0.08480 22400.00 17400.00 0.00 0.08602 22600.00 17400.00 0.00 0.08635 22200.00 17600.00 0.00 0.08635 22400.00 17600.00 0.00 0.08756 22600.00 17600.00 0.00 0.08787 22200.00 17800.00 0.00 0.08794 22400.00 17800.00 0.00 0.08914 22600.00 17800.00 0.00 0.08943 22200.00 18000.00 0.00 0.08948 22400.00 18000.00 0.00 0.09067 22600.00 18000.00 0.00 0.09092 22200.00 18200.00 0.00 0.09101 22400.00 18200.00 0.00 0.09220 22600.00 18200.00 0.00 0.09239 22200.00 18400.00 0.00 0.09265 22400.00 18400.00 0.00 0.09380 22600.00 18400.00 0.00 0.09390 22200.00 18600.00 0.00 0.09427 22400.00 18600.00 0.00 0.09536 22600.00 18600.00 0.00 0.09538 22200.00 18800.00 0.00 0.09596 22400.00 18800.00 0.00 0.09700 22200.00 19000.00 0.00 0.09765 22400.00 19000.00 0.00 0.09866 22200.00 19200.00 0.00 0.09953 22400.00 19200.00 0.00 0.10050 22200.00 19400.00 0.00 0.10140 22400.00 19400.00 0.00 0.10232 22200.00 19600.00 0.00 0.10333 22400.00 19600.00 0.00 0.10422 22200.00 19800.00 0.00 0.10527 22400.00 19800.00 0.00 0.10615 22200.00 20000.00 0.00 0.10739 22400.00 20000.00 0.00 0.10822 22200.00 20200.00 0.00 0.10958 22400.00 20200.00 0.00 0.11030 22200.00 20400.00 0.00 0.11166 22400.00 20400.00 0.00 0.11223 22200.00 20600.00 0.00 0.11374 22400.00 20600.00 0.00 0.11412 22200.00 20800.00 0.00 0.11551 22400.00 20800.00 0.00 0.11571 22000.00 21000.00 0.00 0.11579 22200.00 21000.00 0.00 0.11753 22400.00 21000.00 0.00 0.11758 22000.00 21200.00 0.00 0.11796 22200.00 21200.00 0.00 0.11959 22000.00 21400.00 0.00 0.12016 22200.00 21400.00 0.00 0.12162 22000.00 21600.00 0.00 0.12214 22200.00 21600.00 0.00 0.12342 22000.00 21800.00 0.00 0.12387 22200.00 21800.00 0.00 0.12494 22000.00 22000.00 0.00 0.12583 22200.00 22000.00 0.00 0.12639 22000.00 22200.00 0.00 0.12652 22000.00 22200.00 5.00 0.12661 22000.00 22200.00 10.00 0.12669 22000.00 22200.00 15.00 0.12677 22000.00 22200.00 20.00 0.12684 22000.00 22200.00 25.00 0.12691 22000.00 22200.00 30.00 0.12697 22000.00 22200.00 35.00 0.12702 22000.00 22200.00 40.00 0.12705 22000.00 22200.00 45.00 0.12706 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22035.51 22273.52 41.04 0.12720 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/263.ctf NX, NY, NZ: 512 512 1 MODE : real