CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/262.mrc Output diagnostic file name Micrographs_0601/262.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/262.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2272.000 8292.000 Mean, RMS : 4446.556 218.4284 TITLE 1: emi2mrc: 262.emi Sun Jan 05 20:17:09 2014 TITLE 2: kV 300, Mag 78000x, Def 24940, Spot 4, Int 55.08%, Obj 88.09%, Dif 73.67% TITLE 3: X 63.489, Y 601.918, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00347 10200.00 10000.00 0.00 -0.00313 10400.00 10000.00 0.00 -0.00288 10600.00 10000.00 0.00 -0.00281 10800.00 10000.00 0.00 -0.00260 11000.00 10000.00 0.00 -0.00236 11200.00 10000.00 0.00 -0.00215 11400.00 10000.00 0.00 -0.00188 11600.00 10000.00 0.00 -0.00154 11800.00 10000.00 0.00 -0.00134 12000.00 10000.00 0.00 -0.00126 12200.00 10000.00 0.00 -0.00109 12400.00 10000.00 0.00 -0.00097 12600.00 10000.00 0.00 -0.00075 12800.00 10000.00 0.00 -0.00035 13000.00 10000.00 0.00 0.00012 13200.00 10000.00 0.00 0.00067 13400.00 10000.00 0.00 0.00125 13600.00 10000.00 0.00 0.00182 13800.00 10000.00 0.00 0.00238 14000.00 10000.00 0.00 0.00295 14200.00 10000.00 0.00 0.00364 14400.00 10000.00 0.00 0.00441 14600.00 10000.00 0.00 0.00515 14800.00 10000.00 0.00 0.00584 15000.00 10000.00 0.00 0.00648 15200.00 10000.00 0.00 0.00711 15400.00 10000.00 0.00 0.00765 15600.00 10000.00 0.00 0.00813 15800.00 10000.00 0.00 0.00879 16000.00 10000.00 0.00 0.00962 16200.00 10000.00 0.00 0.01060 16400.00 10000.00 0.00 0.01165 16600.00 10000.00 0.00 0.01265 16800.00 10000.00 0.00 0.01367 17000.00 10000.00 0.00 0.01461 17200.00 10000.00 0.00 0.01546 17400.00 10000.00 0.00 0.01653 17600.00 10000.00 0.00 0.01769 17800.00 10000.00 0.00 0.01891 18000.00 10000.00 0.00 0.02012 18200.00 10000.00 0.00 0.02121 18400.00 10000.00 0.00 0.02240 18600.00 10000.00 0.00 0.02367 18800.00 10000.00 0.00 0.02489 19000.00 10000.00 0.00 0.02606 19200.00 10000.00 0.00 0.02725 19400.00 10000.00 0.00 0.02851 19600.00 10000.00 0.00 0.02968 19800.00 10000.00 0.00 0.03089 20000.00 10000.00 0.00 0.03231 20200.00 10000.00 0.00 0.03380 20400.00 10000.00 0.00 0.03527 20600.00 10000.00 0.00 0.03688 20800.00 10000.00 0.00 0.03859 21000.00 10000.00 0.00 0.04017 21200.00 10000.00 0.00 0.04182 21400.00 10000.00 0.00 0.04360 21600.00 10000.00 0.00 0.04524 21800.00 10000.00 0.00 0.04665 22000.00 10000.00 0.00 0.04790 22200.00 10000.00 0.00 0.04884 22400.00 10000.00 0.00 0.04947 22600.00 10000.00 0.00 0.04987 22800.00 10000.00 0.00 0.05006 22400.00 10200.00 0.00 0.05012 22600.00 10200.00 0.00 0.05052 22800.00 10200.00 0.00 0.05071 22400.00 10400.00 0.00 0.05080 22600.00 10400.00 0.00 0.05120 22800.00 10400.00 0.00 0.05137 22400.00 10600.00 0.00 0.05152 22600.00 10600.00 0.00 0.05189 22800.00 10600.00 0.00 0.05202 22400.00 10800.00 0.00 0.05226 22600.00 10800.00 0.00 0.05257 22800.00 10800.00 0.00 0.05262 22400.00 11000.00 0.00 0.05297 22600.00 11000.00 0.00 0.05320 22800.00 11000.00 0.00 0.05321 22400.00 11200.00 0.00 0.05348 22600.00 11200.00 0.00 0.05366 22800.00 11200.00 0.00 0.05369 22400.00 11400.00 0.00 0.05400 22600.00 11400.00 0.00 0.05418 22800.00 11400.00 0.00 0.05424 22400.00 11600.00 0.00 0.05464 22600.00 11600.00 0.00 0.05482 22800.00 11600.00 0.00 0.05492 22400.00 11800.00 0.00 0.05528 22600.00 11800.00 0.00 0.05548 22800.00 11800.00 0.00 0.05561 22400.00 12000.00 0.00 0.05587 22600.00 12000.00 0.00 0.05608 22800.00 12000.00 0.00 0.05623 22400.00 12200.00 0.00 0.05655 22600.00 12200.00 0.00 0.05677 22800.00 12200.00 0.00 0.05693 22400.00 12400.00 0.00 0.05726 22600.00 12400.00 0.00 0.05750 22800.00 12400.00 0.00 0.05770 22400.00 12600.00 0.00 0.05808 22600.00 12600.00 0.00 0.05836 22800.00 12600.00 0.00 0.05858 22200.00 12800.00 0.00 0.05866 22400.00 12800.00 0.00 0.05916 22600.00 12800.00 0.00 0.05946 22800.00 12800.00 0.00 0.05964 22400.00 13000.00 0.00 0.06010 22600.00 13000.00 0.00 0.06038 22800.00 13000.00 0.00 0.06053 22400.00 13200.00 0.00 0.06097 22600.00 13200.00 0.00 0.06127 22800.00 13200.00 0.00 0.06143 22200.00 13400.00 0.00 0.06145 22400.00 13400.00 0.00 0.06196 22600.00 13400.00 0.00 0.06229 22800.00 13400.00 0.00 0.06244 22200.00 13600.00 0.00 0.06247 22400.00 13600.00 0.00 0.06303 22600.00 13600.00 0.00 0.06336 22800.00 13600.00 0.00 0.06346 22200.00 13800.00 0.00 0.06352 22400.00 13800.00 0.00 0.06407 22600.00 13800.00 0.00 0.06436 22800.00 13800.00 0.00 0.06441 22200.00 14000.00 0.00 0.06462 22400.00 14000.00 0.00 0.06517 22600.00 14000.00 0.00 0.06544 22800.00 14000.00 0.00 0.06545 22200.00 14200.00 0.00 0.06585 22400.00 14200.00 0.00 0.06639 22600.00 14200.00 0.00 0.06661 22200.00 14400.00 0.00 0.06700 22400.00 14400.00 0.00 0.06747 22600.00 14400.00 0.00 0.06763 22200.00 14600.00 0.00 0.06821 22400.00 14600.00 0.00 0.06861 22600.00 14600.00 0.00 0.06874 22200.00 14800.00 0.00 0.06937 22400.00 14800.00 0.00 0.06972 22600.00 14800.00 0.00 0.06985 22200.00 15000.00 0.00 0.07053 22400.00 15000.00 0.00 0.07085 22600.00 15000.00 0.00 0.07100 22200.00 15200.00 0.00 0.07180 22400.00 15200.00 0.00 0.07214 22600.00 15200.00 0.00 0.07232 22200.00 15400.00 0.00 0.07313 22400.00 15400.00 0.00 0.07351 22600.00 15400.00 0.00 0.07368 22000.00 15600.00 0.00 0.07376 22200.00 15600.00 0.00 0.07462 22400.00 15600.00 0.00 0.07500 22600.00 15600.00 0.00 0.07514 22000.00 15800.00 0.00 0.07526 22200.00 15800.00 0.00 0.07611 22400.00 15800.00 0.00 0.07647 22600.00 15800.00 0.00 0.07659 22000.00 16000.00 0.00 0.07678 22200.00 16000.00 0.00 0.07760 22400.00 16000.00 0.00 0.07794 22600.00 16000.00 0.00 0.07804 22000.00 16200.00 0.00 0.07823 22200.00 16200.00 0.00 0.07903 22400.00 16200.00 0.00 0.07936 22600.00 16200.00 0.00 0.07940 22000.00 16400.00 0.00 0.07963 22200.00 16400.00 0.00 0.08041 22400.00 16400.00 0.00 0.08070 22000.00 16600.00 0.00 0.08099 22200.00 16600.00 0.00 0.08173 22400.00 16600.00 0.00 0.08197 22000.00 16800.00 0.00 0.08252 22200.00 16800.00 0.00 0.08324 22400.00 16800.00 0.00 0.08343 22000.00 17000.00 0.00 0.08413 22200.00 17000.00 0.00 0.08481 22400.00 17000.00 0.00 0.08497 22000.00 17200.00 0.00 0.08566 22200.00 17200.00 0.00 0.08629 22400.00 17200.00 0.00 0.08641 22000.00 17400.00 0.00 0.08722 22200.00 17400.00 0.00 0.08783 22400.00 17400.00 0.00 0.08795 22000.00 17600.00 0.00 0.08865 22200.00 17600.00 0.00 0.08927 22400.00 17600.00 0.00 0.08938 22000.00 17800.00 0.00 0.09029 22200.00 17800.00 0.00 0.09088 22400.00 17800.00 0.00 0.09095 22000.00 18000.00 0.00 0.09188 22200.00 18000.00 0.00 0.09244 22400.00 18000.00 0.00 0.09246 22000.00 18200.00 0.00 0.09341 22200.00 18200.00 0.00 0.09395 22400.00 18200.00 0.00 0.09395 22000.00 18400.00 0.00 0.09504 22200.00 18400.00 0.00 0.09558 22000.00 18600.00 0.00 0.09681 22200.00 18600.00 0.00 0.09734 21800.00 18800.00 0.00 0.09740 22000.00 18800.00 0.00 0.09866 22200.00 18800.00 0.00 0.09917 21800.00 19000.00 0.00 0.09922 22000.00 19000.00 0.00 0.10049 22200.00 19000.00 0.00 0.10094 21800.00 19200.00 0.00 0.10127 22000.00 19200.00 0.00 0.10247 22200.00 19200.00 0.00 0.10283 21800.00 19400.00 0.00 0.10310 22000.00 19400.00 0.00 0.10424 22200.00 19400.00 0.00 0.10454 21800.00 19600.00 0.00 0.10500 22000.00 19600.00 0.00 0.10610 22200.00 19600.00 0.00 0.10638 21800.00 19800.00 0.00 0.10689 22000.00 19800.00 0.00 0.10796 22200.00 19800.00 0.00 0.10819 21800.00 20000.00 0.00 0.10864 22000.00 20000.00 0.00 0.10966 22200.00 20000.00 0.00 0.10982 21800.00 20200.00 0.00 0.11046 22000.00 20200.00 0.00 0.11143 22200.00 20200.00 0.00 0.11152 21800.00 20400.00 0.00 0.11228 22000.00 20400.00 0.00 0.11317 22200.00 20400.00 0.00 0.11317 21800.00 20600.00 0.00 0.11418 22000.00 20600.00 0.00 0.11499 21800.00 20800.00 0.00 0.11590 22000.00 20800.00 0.00 0.11664 21800.00 21000.00 0.00 0.11780 22000.00 21000.00 0.00 0.11841 21800.00 21200.00 0.00 0.11956 22000.00 21200.00 0.00 0.12000 21600.00 21400.00 0.00 0.12011 21800.00 21400.00 0.00 0.12125 22000.00 21400.00 0.00 0.12147 21600.00 21600.00 0.00 0.12198 21800.00 21600.00 0.00 0.12274 22000.00 21600.00 0.00 0.12275 21600.00 21800.00 0.00 0.12295 21800.00 21800.00 0.00 0.12355 21800.00 22000.00 0.00 0.12358 21800.00 22000.00 5.00 0.12372 21800.00 22000.00 10.00 0.12385 21600.00 22000.00 15.00 0.12393 21800.00 22000.00 15.00 0.12398 21600.00 22000.00 20.00 0.12414 21600.00 22000.00 25.00 0.12432 21600.00 22000.00 30.00 0.12446 21600.00 22000.00 35.00 0.12456 21600.00 22000.00 40.00 0.12460 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21652.49 22021.40 42.03 0.12467 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/262.ctf NX, NY, NZ: 512 512 1 MODE : real