CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/261.mrc Output diagnostic file name Micrographs_0601/261.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/261.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2442.000 7960.000 Mean, RMS : 4455.335 216.0012 TITLE 1: emi2mrc: 261.emi Sun Jan 05 20:16:23 2014 TITLE 2: kV 300, Mag 78000x, Def 24940, Spot 4, Int 55.08%, Obj 88.09%, Dif 73.67% TITLE 3: X 66.043, Y 601.475, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01627 11200.00 10000.00 0.00 -0.01608 11400.00 10000.00 0.00 -0.01575 11600.00 10000.00 0.00 -0.01538 11800.00 10000.00 0.00 -0.01522 12000.00 10000.00 0.00 -0.01501 12200.00 10000.00 0.00 -0.01468 12400.00 10000.00 0.00 -0.01454 12600.00 10000.00 0.00 -0.01419 12800.00 10000.00 0.00 -0.01387 13000.00 10000.00 0.00 -0.01342 13200.00 10000.00 0.00 -0.01279 13400.00 10000.00 0.00 -0.01228 13600.00 10000.00 0.00 -0.01162 13800.00 10000.00 0.00 -0.01107 14000.00 10000.00 0.00 -0.01042 14200.00 10000.00 0.00 -0.00963 14400.00 10000.00 0.00 -0.00904 14600.00 10000.00 0.00 -0.00856 14800.00 10000.00 0.00 -0.00806 15000.00 10000.00 0.00 -0.00738 15200.00 10000.00 0.00 -0.00659 15400.00 10000.00 0.00 -0.00575 15600.00 10000.00 0.00 -0.00482 15800.00 10000.00 0.00 -0.00392 16000.00 10000.00 0.00 -0.00307 16200.00 10000.00 0.00 -0.00205 16400.00 10000.00 0.00 -0.00086 16600.00 10000.00 0.00 0.00035 16800.00 10000.00 0.00 0.00166 17000.00 10000.00 0.00 0.00284 17200.00 10000.00 0.00 0.00391 17400.00 10000.00 0.00 0.00538 17600.00 10000.00 0.00 0.00692 17800.00 10000.00 0.00 0.00846 18000.00 10000.00 0.00 0.00995 18200.00 10000.00 0.00 0.01119 18400.00 10000.00 0.00 0.01252 18600.00 10000.00 0.00 0.01387 18800.00 10000.00 0.00 0.01518 19000.00 10000.00 0.00 0.01657 19200.00 10000.00 0.00 0.01787 19400.00 10000.00 0.00 0.01921 19600.00 10000.00 0.00 0.02068 19800.00 10000.00 0.00 0.02219 20000.00 10000.00 0.00 0.02378 20200.00 10000.00 0.00 0.02531 20400.00 10000.00 0.00 0.02672 20600.00 10000.00 0.00 0.02819 20800.00 10000.00 0.00 0.02989 21000.00 10000.00 0.00 0.03179 21200.00 10000.00 0.00 0.03371 21400.00 10000.00 0.00 0.03578 21600.00 10000.00 0.00 0.03764 21800.00 10000.00 0.00 0.03896 22000.00 10000.00 0.00 0.04006 22200.00 10000.00 0.00 0.04079 22400.00 10000.00 0.00 0.04123 22600.00 10000.00 0.00 0.04150 22800.00 10000.00 0.00 0.04184 23000.00 10000.00 0.00 0.04220 23200.00 10000.00 0.00 0.04227 22600.00 10200.00 0.00 0.04234 22800.00 10200.00 0.00 0.04269 23000.00 10200.00 0.00 0.04298 22600.00 10400.00 0.00 0.04323 22800.00 10400.00 0.00 0.04353 23000.00 10400.00 0.00 0.04372 22600.00 10600.00 0.00 0.04397 22800.00 10600.00 0.00 0.04420 23000.00 10600.00 0.00 0.04429 22400.00 10800.00 0.00 0.04433 22600.00 10800.00 0.00 0.04455 22800.00 10800.00 0.00 0.04472 23000.00 10800.00 0.00 0.04477 22400.00 11000.00 0.00 0.04487 22600.00 11000.00 0.00 0.04504 22800.00 11000.00 0.00 0.04517 22400.00 11200.00 0.00 0.04520 22600.00 11200.00 0.00 0.04537 22800.00 11200.00 0.00 0.04547 22400.00 11400.00 0.00 0.04557 22600.00 11400.00 0.00 0.04574 22800.00 11400.00 0.00 0.04580 22400.00 11600.00 0.00 0.04593 22600.00 11600.00 0.00 0.04606 22800.00 11600.00 0.00 0.04607 22200.00 11800.00 0.00 0.04613 22400.00 11800.00 0.00 0.04636 22600.00 11800.00 0.00 0.04645 22800.00 11800.00 0.00 0.04647 22200.00 12000.00 0.00 0.04663 22400.00 12000.00 0.00 0.04687 22600.00 12000.00 0.00 0.04700 22800.00 12000.00 0.00 0.04706 22200.00 12200.00 0.00 0.04719 22400.00 12200.00 0.00 0.04751 22600.00 12200.00 0.00 0.04771 22800.00 12200.00 0.00 0.04778 22200.00 12400.00 0.00 0.04808 22400.00 12400.00 0.00 0.04849 22600.00 12400.00 0.00 0.04868 22800.00 12400.00 0.00 0.04871 22200.00 12600.00 0.00 0.04902 22400.00 12600.00 0.00 0.04944 22600.00 12600.00 0.00 0.04957 22800.00 12600.00 0.00 0.04958 23000.00 12600.00 0.00 0.04960 23200.00 12600.00 0.00 0.04964 23400.00 12600.00 0.00 0.04966 22200.00 12800.00 0.00 0.04996 22400.00 12800.00 0.00 0.05035 22600.00 12800.00 0.00 0.05040 22800.00 12800.00 0.00 0.05043 23000.00 12800.00 0.00 0.05049 23200.00 12800.00 0.00 0.05051 22200.00 13000.00 0.00 0.05096 22400.00 13000.00 0.00 0.05127 22600.00 13000.00 0.00 0.05128 22800.00 13000.00 0.00 0.05134 23000.00 13000.00 0.00 0.05140 22200.00 13200.00 0.00 0.05182 22400.00 13200.00 0.00 0.05205 22600.00 13200.00 0.00 0.05208 22800.00 13200.00 0.00 0.05218 23000.00 13200.00 0.00 0.05220 22200.00 13400.00 0.00 0.05258 22400.00 13400.00 0.00 0.05281 22600.00 13400.00 0.00 0.05289 22800.00 13400.00 0.00 0.05300 22200.00 13600.00 0.00 0.05355 22400.00 13600.00 0.00 0.05378 22600.00 13600.00 0.00 0.05385 22800.00 13600.00 0.00 0.05391 22200.00 13800.00 0.00 0.05441 22400.00 13800.00 0.00 0.05459 22600.00 13800.00 0.00 0.05463 22200.00 14000.00 0.00 0.05519 22400.00 14000.00 0.00 0.05535 22600.00 14000.00 0.00 0.05540 22000.00 14200.00 0.00 0.05573 22200.00 14200.00 0.00 0.05632 22400.00 14200.00 0.00 0.05646 22600.00 14200.00 0.00 0.05653 22000.00 14400.00 0.00 0.05680 22200.00 14400.00 0.00 0.05734 22400.00 14400.00 0.00 0.05745 22600.00 14400.00 0.00 0.05752 22000.00 14600.00 0.00 0.05782 22200.00 14600.00 0.00 0.05831 22400.00 14600.00 0.00 0.05844 22600.00 14600.00 0.00 0.05853 22000.00 14800.00 0.00 0.05902 22200.00 14800.00 0.00 0.05949 22400.00 14800.00 0.00 0.05967 22600.00 14800.00 0.00 0.05978 22000.00 15000.00 0.00 0.06030 22200.00 15000.00 0.00 0.06079 22400.00 15000.00 0.00 0.06101 22600.00 15000.00 0.00 0.06108 22000.00 15200.00 0.00 0.06166 22200.00 15200.00 0.00 0.06215 22400.00 15200.00 0.00 0.06237 22600.00 15200.00 0.00 0.06238 22000.00 15400.00 0.00 0.06309 22200.00 15400.00 0.00 0.06354 22400.00 15400.00 0.00 0.06373 22000.00 15600.00 0.00 0.06444 22200.00 15600.00 0.00 0.06487 22400.00 15600.00 0.00 0.06507 22000.00 15800.00 0.00 0.06575 22200.00 15800.00 0.00 0.06620 22400.00 15800.00 0.00 0.06641 22000.00 16000.00 0.00 0.06710 22200.00 16000.00 0.00 0.06756 22400.00 16000.00 0.00 0.06770 22000.00 16200.00 0.00 0.06823 22200.00 16200.00 0.00 0.06868 22400.00 16200.00 0.00 0.06876 22000.00 16400.00 0.00 0.06935 22200.00 16400.00 0.00 0.06981 22400.00 16400.00 0.00 0.06983 22000.00 16600.00 0.00 0.07049 22200.00 16600.00 0.00 0.07094 22400.00 16600.00 0.00 0.07095 22000.00 16800.00 0.00 0.07175 22200.00 16800.00 0.00 0.07225 22000.00 17000.00 0.00 0.07327 22200.00 17000.00 0.00 0.07378 22000.00 17200.00 0.00 0.07486 22200.00 17200.00 0.00 0.07532 22000.00 17400.00 0.00 0.07648 22200.00 17400.00 0.00 0.07684 21800.00 17600.00 0.00 0.07689 22000.00 17600.00 0.00 0.07800 22200.00 17600.00 0.00 0.07826 21800.00 17800.00 0.00 0.07851 22000.00 17800.00 0.00 0.07952 22200.00 17800.00 0.00 0.07970 21800.00 18000.00 0.00 0.07994 22000.00 18000.00 0.00 0.08089 22200.00 18000.00 0.00 0.08107 21800.00 18200.00 0.00 0.08147 22000.00 18200.00 0.00 0.08241 22200.00 18200.00 0.00 0.08259 21800.00 18400.00 0.00 0.08318 22000.00 18400.00 0.00 0.08410 22200.00 18400.00 0.00 0.08425 21800.00 18600.00 0.00 0.08474 22000.00 18600.00 0.00 0.08564 22200.00 18600.00 0.00 0.08574 21800.00 18800.00 0.00 0.08625 22000.00 18800.00 0.00 0.08715 22200.00 18800.00 0.00 0.08723 21800.00 19000.00 0.00 0.08788 22000.00 19000.00 0.00 0.08876 22200.00 19000.00 0.00 0.08881 21800.00 19200.00 0.00 0.08963 22000.00 19200.00 0.00 0.09044 21800.00 19400.00 0.00 0.09113 22000.00 19400.00 0.00 0.09188 21800.00 19600.00 0.00 0.09275 22000.00 19600.00 0.00 0.09351 22200.00 19600.00 0.00 0.09356 21800.00 19800.00 0.00 0.09452 22000.00 19800.00 0.00 0.09536 22200.00 19800.00 0.00 0.09541 21800.00 20000.00 0.00 0.09653 22000.00 20000.00 0.00 0.09735 21800.00 20200.00 0.00 0.09838 22000.00 20200.00 0.00 0.09904 21800.00 20400.00 0.00 0.09992 22000.00 20400.00 0.00 0.10044 21600.00 20600.00 0.00 0.10056 21800.00 20600.00 0.00 0.10175 22000.00 20600.00 0.00 0.10221 21600.00 20800.00 0.00 0.10223 21800.00 20800.00 0.00 0.10335 22000.00 20800.00 0.00 0.10376 21600.00 21000.00 0.00 0.10405 21800.00 21000.00 0.00 0.10509 22000.00 21000.00 0.00 0.10541 21600.00 21200.00 0.00 0.10580 21800.00 21200.00 0.00 0.10676 22000.00 21200.00 0.00 0.10696 21600.00 21400.00 0.00 0.10755 21800.00 21400.00 0.00 0.10835 21600.00 21600.00 0.00 0.10916 21800.00 21600.00 0.00 0.10961 21600.00 21800.00 0.00 0.10974 21800.00 21800.00 0.00 0.11000 21600.00 22000.00 5.00 0.11000 21600.00 22000.00 10.00 0.11012 21600.00 22000.00 15.00 0.11024 21600.00 22000.00 20.00 0.11033 21600.00 22000.00 25.00 0.11041 21600.00 22000.00 30.00 0.11045 21600.00 22000.00 35.00 0.11045 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21625.21 21974.58 35.00 0.11047 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/261.ctf NX, NY, NZ: 512 512 1 MODE : real