CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/259.mrc Output diagnostic file name Micrographs_0601/259.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/259.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2504.000 8218.000 Mean, RMS : 4425.691 215.8606 TITLE 1: emi2mrc: 259.emi Sun Jan 05 20:14:27 2014 TITLE 2: kV 300, Mag 78000x, Def 24610, Spot 4, Int 55.08%, Obj 88.11%, Dif 73.67% TITLE 3: X 71.571, Y 600.980, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04141 10200.00 10000.00 0.00 0.04353 10400.00 10000.00 0.00 0.04533 10600.00 10000.00 0.00 0.04734 10800.00 10000.00 0.00 0.04930 11000.00 10000.00 0.00 0.05149 11200.00 10000.00 0.00 0.05385 11400.00 10000.00 0.00 0.05623 11600.00 10000.00 0.00 0.05882 11800.00 10000.00 0.00 0.06135 12000.00 10000.00 0.00 0.06412 12200.00 10000.00 0.00 0.06705 12400.00 10000.00 0.00 0.07012 12600.00 10000.00 0.00 0.07297 12800.00 10000.00 0.00 0.07584 13000.00 10000.00 0.00 0.07878 13200.00 10000.00 0.00 0.08162 13400.00 10000.00 0.00 0.08478 13600.00 10000.00 0.00 0.08792 13800.00 10000.00 0.00 0.09108 14000.00 10000.00 0.00 0.09406 14200.00 10000.00 0.00 0.09654 14400.00 10000.00 0.00 0.09811 14600.00 10000.00 0.00 0.09876 14800.00 10000.00 0.00 0.09888 14200.00 10200.00 0.00 0.09897 14400.00 10200.00 0.00 0.10047 14600.00 10200.00 0.00 0.10100 14800.00 10200.00 0.00 0.10103 14200.00 10400.00 0.00 0.10152 14400.00 10400.00 0.00 0.10294 14600.00 10400.00 0.00 0.10339 14200.00 10600.00 0.00 0.10421 14400.00 10600.00 0.00 0.10555 14600.00 10600.00 0.00 0.10592 14200.00 10800.00 0.00 0.10676 14400.00 10800.00 0.00 0.10803 14600.00 10800.00 0.00 0.10832 14200.00 11000.00 0.00 0.10941 14400.00 11000.00 0.00 0.11062 14600.00 11000.00 0.00 0.11081 14200.00 11200.00 0.00 0.11203 14400.00 11200.00 0.00 0.11317 14600.00 11200.00 0.00 0.11326 14200.00 11400.00 0.00 0.11494 14400.00 11400.00 0.00 0.11600 14200.00 11600.00 0.00 0.11771 14400.00 11600.00 0.00 0.11870 14200.00 11800.00 0.00 0.12060 14400.00 11800.00 0.00 0.12153 14000.00 12000.00 0.00 0.12158 14200.00 12000.00 0.00 0.12363 14400.00 12000.00 0.00 0.12450 14000.00 12200.00 0.00 0.12468 14200.00 12200.00 0.00 0.12668 14400.00 12200.00 0.00 0.12743 14000.00 12400.00 0.00 0.12777 14200.00 12400.00 0.00 0.12969 14400.00 12400.00 0.00 0.13033 14000.00 12600.00 0.00 0.13071 14200.00 12600.00 0.00 0.13259 14400.00 12600.00 0.00 0.13317 14000.00 12800.00 0.00 0.13413 14200.00 12800.00 0.00 0.13592 14400.00 12800.00 0.00 0.13638 14000.00 13000.00 0.00 0.13713 14200.00 13000.00 0.00 0.13884 14400.00 13000.00 0.00 0.13915 14000.00 13200.00 0.00 0.14047 14200.00 13200.00 0.00 0.14210 14400.00 13200.00 0.00 0.14224 14000.00 13400.00 0.00 0.14383 14200.00 13400.00 0.00 0.14531 14000.00 13600.00 0.00 0.14705 14200.00 13600.00 0.00 0.14821 14000.00 13800.00 0.00 0.14992 14200.00 13800.00 0.00 0.15064 14000.00 14000.00 0.00 0.15127 14200.00 14000.00 0.00 0.15185 14000.00 14200.00 0.00 0.15231 14200.00 14200.00 0.00 0.15241 14000.00 14200.00 10.00 0.15243 14000.00 14200.00 15.00 0.15248 14000.00 14200.00 20.00 0.15251 14000.00 14200.00 25.00 0.15253 14000.00 14200.00 30.00 0.15254 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14086.06 14205.98 24.77 0.15266 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/259.ctf NX, NY, NZ: 512 512 1 MODE : real