CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/258.mrc Output diagnostic file name Micrographs_0601/258.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/258.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2678.000 8058.000 Mean, RMS : 4448.670 220.3010 TITLE 1: emi2mrc: 258.emi Sun Jan 05 20:10:09 2014 TITLE 2: kV 300, Mag 78000x, Def 24940, Spot 4, Int 55.08%, Obj 88.05%, Dif 73.67% TITLE 3: X 33.045, Y 514.139, Z -81.545, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00859 11600.00 10000.00 0.00 -0.00847 11800.00 10000.00 0.00 -0.00837 12000.00 10000.00 0.00 -0.00833 12200.00 10000.00 0.00 -0.00812 12400.00 10000.00 0.00 -0.00780 12600.00 10000.00 0.00 -0.00742 12800.00 10000.00 0.00 -0.00701 13000.00 10000.00 0.00 -0.00669 13200.00 10000.00 0.00 -0.00642 13400.00 10000.00 0.00 -0.00612 13600.00 10000.00 0.00 -0.00577 13800.00 10000.00 0.00 -0.00542 14000.00 10000.00 0.00 -0.00508 14200.00 10000.00 0.00 -0.00453 14400.00 10000.00 0.00 -0.00389 14600.00 10000.00 0.00 -0.00328 14800.00 10000.00 0.00 -0.00261 15000.00 10000.00 0.00 -0.00196 15200.00 10000.00 0.00 -0.00134 15400.00 10000.00 0.00 -0.00072 15600.00 10000.00 0.00 -0.00010 15800.00 10000.00 0.00 0.00043 16000.00 10000.00 0.00 0.00095 16200.00 10000.00 0.00 0.00159 16400.00 10000.00 0.00 0.00243 16600.00 10000.00 0.00 0.00331 16800.00 10000.00 0.00 0.00409 17000.00 10000.00 0.00 0.00492 17200.00 10000.00 0.00 0.00571 17400.00 10000.00 0.00 0.00660 17600.00 10000.00 0.00 0.00768 17800.00 10000.00 0.00 0.00863 18000.00 10000.00 0.00 0.00966 18200.00 10000.00 0.00 0.01086 18400.00 10000.00 0.00 0.01192 18600.00 10000.00 0.00 0.01283 18800.00 10000.00 0.00 0.01392 19000.00 10000.00 0.00 0.01497 19200.00 10000.00 0.00 0.01590 19400.00 10000.00 0.00 0.01712 19600.00 10000.00 0.00 0.01849 19800.00 10000.00 0.00 0.01981 20000.00 10000.00 0.00 0.02123 20200.00 10000.00 0.00 0.02273 20400.00 10000.00 0.00 0.02417 20600.00 10000.00 0.00 0.02574 20800.00 10000.00 0.00 0.02747 21000.00 10000.00 0.00 0.02903 21200.00 10000.00 0.00 0.03062 21400.00 10000.00 0.00 0.03231 21600.00 10000.00 0.00 0.03400 21800.00 10000.00 0.00 0.03587 22000.00 10000.00 0.00 0.03788 22200.00 10000.00 0.00 0.03983 22400.00 10000.00 0.00 0.04154 22600.00 10000.00 0.00 0.04269 22800.00 10000.00 0.00 0.04328 23000.00 10000.00 0.00 0.04344 22800.00 10200.00 0.00 0.04371 23000.00 10200.00 0.00 0.04381 22800.00 10400.00 0.00 0.04418 23000.00 10400.00 0.00 0.04423 22800.00 10600.00 0.00 0.04467 23000.00 10600.00 0.00 0.04468 22600.00 10800.00 0.00 0.04469 22800.00 10800.00 0.00 0.04511 23000.00 10800.00 0.00 0.04515 22600.00 11000.00 0.00 0.04524 22800.00 11000.00 0.00 0.04567 23000.00 11000.00 0.00 0.04575 22600.00 11200.00 0.00 0.04585 22800.00 11200.00 0.00 0.04630 23000.00 11200.00 0.00 0.04638 22600.00 11400.00 0.00 0.04658 22800.00 11400.00 0.00 0.04701 23000.00 11400.00 0.00 0.04708 22600.00 11600.00 0.00 0.04730 22800.00 11600.00 0.00 0.04772 23000.00 11600.00 0.00 0.04778 22600.00 11800.00 0.00 0.04806 22800.00 11800.00 0.00 0.04850 23000.00 11800.00 0.00 0.04854 22600.00 12000.00 0.00 0.04895 22800.00 12000.00 0.00 0.04939 23000.00 12000.00 0.00 0.04942 22600.00 12200.00 0.00 0.04978 22800.00 12200.00 0.00 0.05021 23000.00 12200.00 0.00 0.05022 22600.00 12400.00 0.00 0.05057 22800.00 12400.00 0.00 0.05101 23000.00 12400.00 0.00 0.05102 22600.00 12600.00 0.00 0.05130 22800.00 12600.00 0.00 0.05171 22600.00 12800.00 0.00 0.05181 22800.00 12800.00 0.00 0.05217 22600.00 13000.00 0.00 0.05225 22800.00 13000.00 0.00 0.05261 22600.00 13200.00 0.00 0.05290 22800.00 13200.00 0.00 0.05327 22600.00 13400.00 0.00 0.05367 22800.00 13400.00 0.00 0.05403 22600.00 13600.00 0.00 0.05454 22800.00 13600.00 0.00 0.05487 22600.00 13800.00 0.00 0.05534 22800.00 13800.00 0.00 0.05564 22600.00 14000.00 0.00 0.05621 22800.00 14000.00 0.00 0.05648 22600.00 14200.00 0.00 0.05728 22800.00 14200.00 0.00 0.05756 22600.00 14400.00 0.00 0.05838 22800.00 14400.00 0.00 0.05866 22600.00 14600.00 0.00 0.05962 22800.00 14600.00 0.00 0.05988 22600.00 14800.00 0.00 0.06079 22800.00 14800.00 0.00 0.06103 22600.00 15000.00 0.00 0.06210 22800.00 15000.00 0.00 0.06235 22400.00 15200.00 0.00 0.06239 22600.00 15200.00 0.00 0.06357 22800.00 15200.00 0.00 0.06382 22400.00 15400.00 0.00 0.06386 22600.00 15400.00 0.00 0.06505 22800.00 15400.00 0.00 0.06526 22400.00 15600.00 0.00 0.06538 22600.00 15600.00 0.00 0.06652 22800.00 15600.00 0.00 0.06665 22400.00 15800.00 0.00 0.06671 22600.00 15800.00 0.00 0.06776 22800.00 15800.00 0.00 0.06780 22400.00 16000.00 0.00 0.06808 22600.00 16000.00 0.00 0.06904 22400.00 16200.00 0.00 0.06950 22600.00 16200.00 0.00 0.07039 22800.00 16200.00 0.00 0.07039 22400.00 16400.00 0.00 0.07106 22600.00 16400.00 0.00 0.07190 22800.00 16400.00 0.00 0.07193 22400.00 16600.00 0.00 0.07259 22600.00 16600.00 0.00 0.07343 22800.00 16600.00 0.00 0.07348 22400.00 16800.00 0.00 0.07411 22600.00 16800.00 0.00 0.07497 22800.00 16800.00 0.00 0.07505 22400.00 17000.00 0.00 0.07570 22600.00 17000.00 0.00 0.07657 22800.00 17000.00 0.00 0.07665 22400.00 17200.00 0.00 0.07721 22600.00 17200.00 0.00 0.07808 22800.00 17200.00 0.00 0.07815 22400.00 17400.00 0.00 0.07892 22600.00 17400.00 0.00 0.07978 22800.00 17400.00 0.00 0.07982 22400.00 17600.00 0.00 0.08063 22600.00 17600.00 0.00 0.08145 22800.00 17600.00 0.00 0.08146 22400.00 17800.00 0.00 0.08235 22600.00 17800.00 0.00 0.08311 22400.00 18000.00 0.00 0.08404 22600.00 18000.00 0.00 0.08481 22800.00 18000.00 0.00 0.08482 22400.00 18200.00 0.00 0.08579 22600.00 18200.00 0.00 0.08660 22400.00 18400.00 0.00 0.08752 22600.00 18400.00 0.00 0.08833 22400.00 18600.00 0.00 0.08930 22600.00 18600.00 0.00 0.09009 22400.00 18800.00 0.00 0.09132 22600.00 18800.00 0.00 0.09206 22400.00 19000.00 0.00 0.09315 22600.00 19000.00 0.00 0.09379 22400.00 19200.00 0.00 0.09490 22600.00 19200.00 0.00 0.09544 22400.00 19400.00 0.00 0.09664 22600.00 19400.00 0.00 0.09713 22400.00 19600.00 0.00 0.09852 22600.00 19600.00 0.00 0.09894 22400.00 19800.00 0.00 0.10051 22600.00 19800.00 0.00 0.10088 22200.00 20000.00 0.00 0.10096 22400.00 20000.00 0.00 0.10251 22600.00 20000.00 0.00 0.10285 22200.00 20200.00 0.00 0.10307 22400.00 20200.00 0.00 0.10461 22600.00 20200.00 0.00 0.10495 22200.00 20400.00 0.00 0.10512 22400.00 20400.00 0.00 0.10665 22600.00 20400.00 0.00 0.10694 22200.00 20600.00 0.00 0.10725 22400.00 20600.00 0.00 0.10874 22600.00 20600.00 0.00 0.10894 22200.00 20800.00 0.00 0.10948 22400.00 20800.00 0.00 0.11091 22600.00 20800.00 0.00 0.11099 22200.00 21000.00 0.00 0.11184 22400.00 21000.00 0.00 0.11317 22200.00 21200.00 0.00 0.11412 22400.00 21200.00 0.00 0.11535 22200.00 21400.00 0.00 0.11660 22400.00 21400.00 0.00 0.11769 22200.00 21600.00 0.00 0.11895 22400.00 21600.00 0.00 0.11984 22200.00 21800.00 0.00 0.12113 22400.00 21800.00 0.00 0.12175 22200.00 22000.00 0.00 0.12317 22400.00 22000.00 0.00 0.12343 22200.00 22200.00 0.00 0.12402 22400.00 22200.00 0.00 0.12408 22200.00 22400.00 0.00 0.12434 22200.00 22400.00 5.00 0.12444 22200.00 22400.00 10.00 0.12453 22200.00 22400.00 15.00 0.12462 22200.00 22400.00 20.00 0.12469 22200.00 22400.00 25.00 0.12475 22200.00 22400.00 30.00 0.12479 22200.00 22400.00 35.00 0.12482 22200.00 22400.00 40.00 0.12482 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22110.54 22455.57 40.00 0.12493 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/258.ctf NX, NY, NZ: 512 512 1 MODE : real