CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/257.mrc Output diagnostic file name Micrographs_0601/257.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/257.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2318.000 8604.000 Mean, RMS : 4506.995 219.9607 TITLE 1: emi2mrc: 257.emi Sun Jan 05 20:06:12 2014 TITLE 2: kV 300, Mag 78000x, Def 27570, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 33.509, Y 516.287, Z -81.545, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01318 11000.00 10000.00 0.00 -0.01298 11200.00 10000.00 0.00 -0.01265 11400.00 10000.00 0.00 -0.01232 11600.00 10000.00 0.00 -0.01207 11800.00 10000.00 0.00 -0.01183 12000.00 10000.00 0.00 -0.01161 12200.00 10000.00 0.00 -0.01134 12400.00 10000.00 0.00 -0.01107 12600.00 10000.00 0.00 -0.01075 12800.00 10000.00 0.00 -0.01052 13000.00 10000.00 0.00 -0.01031 13200.00 10000.00 0.00 -0.01003 13400.00 10000.00 0.00 -0.00969 13600.00 10000.00 0.00 -0.00933 13800.00 10000.00 0.00 -0.00894 14000.00 10000.00 0.00 -0.00834 14200.00 10000.00 0.00 -0.00772 14400.00 10000.00 0.00 -0.00716 14600.00 10000.00 0.00 -0.00675 14800.00 10000.00 0.00 -0.00634 15000.00 10000.00 0.00 -0.00561 15200.00 10000.00 0.00 -0.00479 15400.00 10000.00 0.00 -0.00412 15600.00 10000.00 0.00 -0.00339 15800.00 10000.00 0.00 -0.00253 16000.00 10000.00 0.00 -0.00163 16200.00 10000.00 0.00 -0.00075 16400.00 10000.00 0.00 0.00025 16600.00 10000.00 0.00 0.00126 16800.00 10000.00 0.00 0.00216 17000.00 10000.00 0.00 0.00316 17200.00 10000.00 0.00 0.00428 17400.00 10000.00 0.00 0.00536 17600.00 10000.00 0.00 0.00640 17800.00 10000.00 0.00 0.00749 18000.00 10000.00 0.00 0.00880 18200.00 10000.00 0.00 0.01006 18400.00 10000.00 0.00 0.01110 18600.00 10000.00 0.00 0.01210 18800.00 10000.00 0.00 0.01325 19000.00 10000.00 0.00 0.01448 19200.00 10000.00 0.00 0.01565 19400.00 10000.00 0.00 0.01696 19600.00 10000.00 0.00 0.01833 19800.00 10000.00 0.00 0.01970 20000.00 10000.00 0.00 0.02118 20200.00 10000.00 0.00 0.02266 20400.00 10000.00 0.00 0.02431 20600.00 10000.00 0.00 0.02609 20800.00 10000.00 0.00 0.02779 21000.00 10000.00 0.00 0.02965 21200.00 10000.00 0.00 0.03154 21400.00 10000.00 0.00 0.03345 21600.00 10000.00 0.00 0.03562 21800.00 10000.00 0.00 0.03773 22000.00 10000.00 0.00 0.03990 22200.00 10000.00 0.00 0.04221 22400.00 10000.00 0.00 0.04404 22600.00 10000.00 0.00 0.04500 22800.00 10000.00 0.00 0.04539 23000.00 10000.00 0.00 0.04556 22600.00 10200.00 0.00 0.04558 22800.00 10200.00 0.00 0.04599 23000.00 10200.00 0.00 0.04616 22600.00 10400.00 0.00 0.04621 22800.00 10400.00 0.00 0.04658 23000.00 10400.00 0.00 0.04673 22600.00 10600.00 0.00 0.04681 22800.00 10600.00 0.00 0.04715 23000.00 10600.00 0.00 0.04729 22600.00 10800.00 0.00 0.04733 22800.00 10800.00 0.00 0.04769 23000.00 10800.00 0.00 0.04785 22600.00 11000.00 0.00 0.04789 22800.00 11000.00 0.00 0.04828 23000.00 11000.00 0.00 0.04844 22600.00 11200.00 0.00 0.04852 22800.00 11200.00 0.00 0.04891 23000.00 11200.00 0.00 0.04906 22600.00 11400.00 0.00 0.04927 22800.00 11400.00 0.00 0.04965 23000.00 11400.00 0.00 0.04979 22600.00 11600.00 0.00 0.04991 22800.00 11600.00 0.00 0.05028 23000.00 11600.00 0.00 0.05039 22600.00 11800.00 0.00 0.05053 22800.00 11800.00 0.00 0.05088 23000.00 11800.00 0.00 0.05100 22600.00 12000.00 0.00 0.05121 22800.00 12000.00 0.00 0.05157 23000.00 12000.00 0.00 0.05173 22600.00 12200.00 0.00 0.05187 22800.00 12200.00 0.00 0.05226 23000.00 12200.00 0.00 0.05243 22600.00 12400.00 0.00 0.05258 22800.00 12400.00 0.00 0.05303 23000.00 12400.00 0.00 0.05317 22600.00 12600.00 0.00 0.05345 22800.00 12600.00 0.00 0.05392 23000.00 12600.00 0.00 0.05402 22600.00 12800.00 0.00 0.05428 22800.00 12800.00 0.00 0.05469 23000.00 12800.00 0.00 0.05471 22600.00 13000.00 0.00 0.05492 22800.00 13000.00 0.00 0.05524 22600.00 13200.00 0.00 0.05570 22800.00 13200.00 0.00 0.05597 22600.00 13400.00 0.00 0.05667 22800.00 13400.00 0.00 0.05690 22600.00 13600.00 0.00 0.05779 22800.00 13600.00 0.00 0.05798 22400.00 13800.00 0.00 0.05799 22600.00 13800.00 0.00 0.05889 22800.00 13800.00 0.00 0.05907 22400.00 14000.00 0.00 0.05917 22600.00 14000.00 0.00 0.06003 22800.00 14000.00 0.00 0.06021 22400.00 14200.00 0.00 0.06045 22600.00 14200.00 0.00 0.06129 22800.00 14200.00 0.00 0.06145 22400.00 14400.00 0.00 0.06154 22600.00 14400.00 0.00 0.06235 22800.00 14400.00 0.00 0.06246 22400.00 14600.00 0.00 0.06258 22600.00 14600.00 0.00 0.06334 22800.00 14600.00 0.00 0.06339 22400.00 14800.00 0.00 0.06356 22600.00 14800.00 0.00 0.06431 22800.00 14800.00 0.00 0.06436 22400.00 15000.00 0.00 0.06477 22600.00 15000.00 0.00 0.06554 22800.00 15000.00 0.00 0.06562 22400.00 15200.00 0.00 0.06609 22600.00 15200.00 0.00 0.06683 22800.00 15200.00 0.00 0.06683 22400.00 15400.00 0.00 0.06728 22600.00 15400.00 0.00 0.06794 22400.00 15600.00 0.00 0.06850 22600.00 15600.00 0.00 0.06913 22400.00 15800.00 0.00 0.06969 22600.00 15800.00 0.00 0.07032 22400.00 16000.00 0.00 0.07106 22600.00 16000.00 0.00 0.07173 22400.00 16200.00 0.00 0.07244 22600.00 16200.00 0.00 0.07308 22400.00 16400.00 0.00 0.07374 22600.00 16400.00 0.00 0.07435 22400.00 16600.00 0.00 0.07522 22600.00 16600.00 0.00 0.07583 22400.00 16800.00 0.00 0.07688 22600.00 16800.00 0.00 0.07746 22400.00 17000.00 0.00 0.07845 22600.00 17000.00 0.00 0.07896 22400.00 17200.00 0.00 0.07993 22600.00 17200.00 0.00 0.08038 22400.00 17400.00 0.00 0.08157 22600.00 17400.00 0.00 0.08201 22400.00 17600.00 0.00 0.08319 22600.00 17600.00 0.00 0.08361 22400.00 17800.00 0.00 0.08491 22600.00 17800.00 0.00 0.08528 22400.00 18000.00 0.00 0.08659 22600.00 18000.00 0.00 0.08693 22400.00 18200.00 0.00 0.08823 22600.00 18200.00 0.00 0.08851 22400.00 18400.00 0.00 0.08985 22600.00 18400.00 0.00 0.09005 22400.00 18600.00 0.00 0.09155 22600.00 18600.00 0.00 0.09169 22400.00 18800.00 0.00 0.09335 22600.00 18800.00 0.00 0.09344 22200.00 19000.00 0.00 0.09345 22400.00 19000.00 0.00 0.09514 22600.00 19000.00 0.00 0.09514 22200.00 19200.00 0.00 0.09546 22400.00 19200.00 0.00 0.09704 22200.00 19400.00 0.00 0.09715 22400.00 19400.00 0.00 0.09864 22200.00 19600.00 0.00 0.09881 22400.00 19600.00 0.00 0.10029 22200.00 19800.00 0.00 0.10064 22400.00 19800.00 0.00 0.10211 22200.00 20000.00 0.00 0.10249 22400.00 20000.00 0.00 0.10389 22200.00 20200.00 0.00 0.10421 22400.00 20200.00 0.00 0.10549 22200.00 20400.00 0.00 0.10590 22400.00 20400.00 0.00 0.10707 22200.00 20600.00 0.00 0.10784 22400.00 20600.00 0.00 0.10890 22200.00 20800.00 0.00 0.10954 22400.00 20800.00 0.00 0.11043 22200.00 21000.00 0.00 0.11118 22400.00 21000.00 0.00 0.11188 22200.00 21200.00 0.00 0.11260 22400.00 21200.00 0.00 0.11312 22200.00 21400.00 0.00 0.11404 22400.00 21400.00 0.00 0.11434 22200.00 21600.00 0.00 0.11535 22400.00 21600.00 0.00 0.11539 22200.00 21800.00 0.00 0.11629 22200.00 22000.00 0.00 0.11731 22200.00 22200.00 0.00 0.11766 22200.00 22400.00 5.00 0.11774 22200.00 22400.00 10.00 0.11783 22200.00 22400.00 15.00 0.11790 22200.00 22400.00 20.00 0.11797 22200.00 22400.00 25.00 0.11801 22200.00 22400.00 30.00 0.11803 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22118.30 22394.51 39.90 0.11816 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/257.ctf NX, NY, NZ: 512 512 1 MODE : real