CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/254.mrc Output diagnostic file name Micrographs_0601/254.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/254.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2552.000 8222.000 Mean, RMS : 4506.055 222.1266 TITLE 1: emi2mrc: 254.emi Sun Jan 05 20:06:50 2014 TITLE 2: kV 300, Mag 78000x, Def 27570, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 36.025, Y 515.811, Z -81.545, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01186 11800.00 10000.00 0.00 -0.01184 12000.00 10000.00 0.00 -0.01179 12200.00 10000.00 0.00 -0.01173 12400.00 10000.00 0.00 -0.01170 12600.00 10000.00 0.00 -0.01157 12800.00 10000.00 0.00 -0.01129 13000.00 10000.00 0.00 -0.01099 13200.00 10000.00 0.00 -0.01080 13400.00 10000.00 0.00 -0.01066 13600.00 10000.00 0.00 -0.01031 13800.00 10000.00 0.00 -0.00989 14000.00 10000.00 0.00 -0.00948 14200.00 10000.00 0.00 -0.00895 14400.00 10000.00 0.00 -0.00829 14600.00 10000.00 0.00 -0.00764 14800.00 10000.00 0.00 -0.00704 15000.00 10000.00 0.00 -0.00635 15200.00 10000.00 0.00 -0.00559 15400.00 10000.00 0.00 -0.00489 15600.00 10000.00 0.00 -0.00427 15800.00 10000.00 0.00 -0.00357 16000.00 10000.00 0.00 -0.00290 16200.00 10000.00 0.00 -0.00225 16400.00 10000.00 0.00 -0.00141 16600.00 10000.00 0.00 -0.00049 16800.00 10000.00 0.00 0.00040 17000.00 10000.00 0.00 0.00135 17200.00 10000.00 0.00 0.00240 17400.00 10000.00 0.00 0.00347 17600.00 10000.00 0.00 0.00459 17800.00 10000.00 0.00 0.00575 18000.00 10000.00 0.00 0.00693 18200.00 10000.00 0.00 0.00816 18400.00 10000.00 0.00 0.00934 18600.00 10000.00 0.00 0.01051 18800.00 10000.00 0.00 0.01180 19000.00 10000.00 0.00 0.01304 19200.00 10000.00 0.00 0.01430 19400.00 10000.00 0.00 0.01561 19600.00 10000.00 0.00 0.01686 19800.00 10000.00 0.00 0.01813 20000.00 10000.00 0.00 0.01948 20200.00 10000.00 0.00 0.02094 20400.00 10000.00 0.00 0.02256 20600.00 10000.00 0.00 0.02428 20800.00 10000.00 0.00 0.02599 21000.00 10000.00 0.00 0.02785 21200.00 10000.00 0.00 0.02983 21400.00 10000.00 0.00 0.03188 21600.00 10000.00 0.00 0.03398 21800.00 10000.00 0.00 0.03606 22000.00 10000.00 0.00 0.03833 22200.00 10000.00 0.00 0.04055 22400.00 10000.00 0.00 0.04217 22600.00 10000.00 0.00 0.04294 22800.00 10000.00 0.00 0.04312 23000.00 10000.00 0.00 0.04317 22600.00 10200.00 0.00 0.04339 22800.00 10200.00 0.00 0.04354 23000.00 10200.00 0.00 0.04357 22600.00 10400.00 0.00 0.04377 22800.00 10400.00 0.00 0.04391 23000.00 10400.00 0.00 0.04395 23200.00 10400.00 0.00 0.04396 22600.00 10600.00 0.00 0.04422 22800.00 10600.00 0.00 0.04436 23000.00 10600.00 0.00 0.04442 23200.00 10600.00 0.00 0.04445 22600.00 10800.00 0.00 0.04472 22800.00 10800.00 0.00 0.04486 23000.00 10800.00 0.00 0.04493 23200.00 10800.00 0.00 0.04496 22600.00 11000.00 0.00 0.04532 22800.00 11000.00 0.00 0.04544 23000.00 11000.00 0.00 0.04552 23200.00 11000.00 0.00 0.04555 22600.00 11200.00 0.00 0.04578 22800.00 11200.00 0.00 0.04591 23000.00 11200.00 0.00 0.04601 23200.00 11200.00 0.00 0.04607 22600.00 11400.00 0.00 0.04620 22800.00 11400.00 0.00 0.04638 23000.00 11400.00 0.00 0.04654 23200.00 11400.00 0.00 0.04663 22600.00 11600.00 0.00 0.04687 22800.00 11600.00 0.00 0.04712 23000.00 11600.00 0.00 0.04733 23200.00 11600.00 0.00 0.04743 22600.00 11800.00 0.00 0.04778 22800.00 11800.00 0.00 0.04805 23000.00 11800.00 0.00 0.04827 23200.00 11800.00 0.00 0.04834 22600.00 12000.00 0.00 0.04863 22800.00 12000.00 0.00 0.04893 23000.00 12000.00 0.00 0.04916 23200.00 12000.00 0.00 0.04921 22600.00 12200.00 0.00 0.04951 22800.00 12200.00 0.00 0.04985 23000.00 12200.00 0.00 0.05008 23200.00 12200.00 0.00 0.05008 22600.00 12400.00 0.00 0.05033 22800.00 12400.00 0.00 0.05067 23000.00 12400.00 0.00 0.05085 22600.00 12600.00 0.00 0.05108 22800.00 12600.00 0.00 0.05138 23000.00 12600.00 0.00 0.05151 22600.00 12800.00 0.00 0.05200 22800.00 12800.00 0.00 0.05224 23000.00 12800.00 0.00 0.05234 22600.00 13000.00 0.00 0.05276 22800.00 13000.00 0.00 0.05297 23000.00 13000.00 0.00 0.05307 22600.00 13200.00 0.00 0.05350 22800.00 13200.00 0.00 0.05375 23000.00 13200.00 0.00 0.05388 22600.00 13400.00 0.00 0.05433 22800.00 13400.00 0.00 0.05465 23000.00 13400.00 0.00 0.05478 22600.00 13600.00 0.00 0.05531 22800.00 13600.00 0.00 0.05568 23000.00 13600.00 0.00 0.05578 22600.00 13800.00 0.00 0.05648 22800.00 13800.00 0.00 0.05684 23000.00 13800.00 0.00 0.05689 22600.00 14000.00 0.00 0.05764 22800.00 14000.00 0.00 0.05794 22600.00 14200.00 0.00 0.05878 22800.00 14200.00 0.00 0.05903 22600.00 14400.00 0.00 0.05983 22800.00 14400.00 0.00 0.06009 23000.00 14400.00 0.00 0.06009 22400.00 14600.00 0.00 0.06013 22600.00 14600.00 0.00 0.06106 22800.00 14600.00 0.00 0.06135 23000.00 14600.00 0.00 0.06137 22400.00 14800.00 0.00 0.06144 22600.00 14800.00 0.00 0.06243 22800.00 14800.00 0.00 0.06272 23000.00 14800.00 0.00 0.06274 22400.00 15000.00 0.00 0.06292 22600.00 15000.00 0.00 0.06393 22800.00 15000.00 0.00 0.06418 22400.00 15200.00 0.00 0.06436 22600.00 15200.00 0.00 0.06537 22800.00 15200.00 0.00 0.06557 22400.00 15400.00 0.00 0.06573 22600.00 15400.00 0.00 0.06674 22800.00 15400.00 0.00 0.06690 22400.00 15600.00 0.00 0.06734 22600.00 15600.00 0.00 0.06827 22800.00 15600.00 0.00 0.06834 22400.00 15800.00 0.00 0.06867 22600.00 15800.00 0.00 0.06950 22800.00 15800.00 0.00 0.06950 22400.00 16000.00 0.00 0.06987 22600.00 16000.00 0.00 0.07068 22800.00 16000.00 0.00 0.07068 22400.00 16200.00 0.00 0.07123 22600.00 16200.00 0.00 0.07200 22400.00 16400.00 0.00 0.07267 22600.00 16400.00 0.00 0.07340 22400.00 16600.00 0.00 0.07425 22600.00 16600.00 0.00 0.07494 22400.00 16800.00 0.00 0.07589 22600.00 16800.00 0.00 0.07660 22400.00 17000.00 0.00 0.07745 22600.00 17000.00 0.00 0.07820 22400.00 17200.00 0.00 0.07896 22600.00 17200.00 0.00 0.07972 22400.00 17400.00 0.00 0.08062 22600.00 17400.00 0.00 0.08133 22400.00 17600.00 0.00 0.08223 22600.00 17600.00 0.00 0.08286 22400.00 17800.00 0.00 0.08380 22600.00 17800.00 0.00 0.08430 22400.00 18000.00 0.00 0.08527 22600.00 18000.00 0.00 0.08566 22400.00 18200.00 0.00 0.08680 22600.00 18200.00 0.00 0.08715 22400.00 18400.00 0.00 0.08847 22600.00 18400.00 0.00 0.08878 22400.00 18600.00 0.00 0.09018 22600.00 18600.00 0.00 0.09044 22400.00 18800.00 0.00 0.09189 22600.00 18800.00 0.00 0.09212 22400.00 19000.00 0.00 0.09359 22600.00 19000.00 0.00 0.09378 22400.00 19200.00 0.00 0.09541 22600.00 19200.00 0.00 0.09555 22400.00 19400.00 0.00 0.09718 22600.00 19400.00 0.00 0.09725 22200.00 19600.00 0.00 0.09742 22400.00 19600.00 0.00 0.09901 22200.00 19800.00 0.00 0.09928 22400.00 19800.00 0.00 0.10079 22200.00 20000.00 0.00 0.10113 22400.00 20000.00 0.00 0.10255 22200.00 20200.00 0.00 0.10303 22400.00 20200.00 0.00 0.10442 22200.00 20400.00 0.00 0.10496 22400.00 20400.00 0.00 0.10636 22200.00 20600.00 0.00 0.10714 22400.00 20600.00 0.00 0.10849 22200.00 20800.00 0.00 0.10914 22400.00 20800.00 0.00 0.11040 22200.00 21000.00 0.00 0.11114 22400.00 21000.00 0.00 0.11232 22200.00 21200.00 0.00 0.11309 22400.00 21200.00 0.00 0.11422 22200.00 21400.00 0.00 0.11520 22400.00 21400.00 0.00 0.11622 22200.00 21600.00 0.00 0.11714 22400.00 21600.00 0.00 0.11793 22200.00 21800.00 0.00 0.11862 22400.00 21800.00 0.00 0.11910 22200.00 22000.00 0.00 0.12013 22400.00 22000.00 0.00 0.12019 22200.00 22200.00 0.00 0.12102 22200.00 22400.00 0.00 0.12129 22200.00 22400.00 5.00 0.12143 22200.00 22400.00 10.00 0.12155 22200.00 22400.00 15.00 0.12166 22200.00 22400.00 20.00 0.12175 22200.00 22400.00 25.00 0.12182 22200.00 22400.00 30.00 0.12185 22200.00 22400.00 35.00 0.12186 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22099.85 22400.00 38.36 0.12205 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/254.ctf NX, NY, NZ: 512 512 1 MODE : real