CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/253.mrc Output diagnostic file name Micrographs_0601/253.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/253.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2322.000 8746.000 Mean, RMS : 4508.442 221.4153 TITLE 1: emi2mrc: 253.emi Sun Jan 05 20:04:50 2014 TITLE 2: kV 300, Mag 78000x, Def 27570, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 30.632, Y 519.796, Z -81.545, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01222 13000.00 10000.00 0.00 -0.01198 13200.00 10000.00 0.00 -0.01173 13400.00 10000.00 0.00 -0.01145 13600.00 10000.00 0.00 -0.01111 13800.00 10000.00 0.00 -0.01078 14000.00 10000.00 0.00 -0.01044 14200.00 10000.00 0.00 -0.01007 14400.00 10000.00 0.00 -0.00972 14600.00 10000.00 0.00 -0.00949 14800.00 10000.00 0.00 -0.00924 15000.00 10000.00 0.00 -0.00898 15200.00 10000.00 0.00 -0.00868 15400.00 10000.00 0.00 -0.00822 15600.00 10000.00 0.00 -0.00772 15800.00 10000.00 0.00 -0.00719 16000.00 10000.00 0.00 -0.00662 16200.00 10000.00 0.00 -0.00599 16400.00 10000.00 0.00 -0.00531 16600.00 10000.00 0.00 -0.00456 16800.00 10000.00 0.00 -0.00364 17000.00 10000.00 0.00 -0.00262 17200.00 10000.00 0.00 -0.00170 17400.00 10000.00 0.00 -0.00089 17600.00 10000.00 0.00 -0.00006 17800.00 10000.00 0.00 0.00085 18000.00 10000.00 0.00 0.00182 18200.00 10000.00 0.00 0.00276 18400.00 10000.00 0.00 0.00364 18600.00 10000.00 0.00 0.00457 18800.00 10000.00 0.00 0.00556 19000.00 10000.00 0.00 0.00640 19200.00 10000.00 0.00 0.00731 19400.00 10000.00 0.00 0.00832 19600.00 10000.00 0.00 0.00920 19800.00 10000.00 0.00 0.01009 20000.00 10000.00 0.00 0.01098 20200.00 10000.00 0.00 0.01211 20400.00 10000.00 0.00 0.01334 20600.00 10000.00 0.00 0.01446 20800.00 10000.00 0.00 0.01567 21000.00 10000.00 0.00 0.01677 21200.00 10000.00 0.00 0.01782 21400.00 10000.00 0.00 0.01888 21600.00 10000.00 0.00 0.01990 21800.00 10000.00 0.00 0.02111 22000.00 10000.00 0.00 0.02241 22200.00 10000.00 0.00 0.02377 22400.00 10000.00 0.00 0.02527 22600.00 10000.00 0.00 0.02687 22800.00 10000.00 0.00 0.02860 23000.00 10000.00 0.00 0.03024 23200.00 10000.00 0.00 0.03168 23400.00 10000.00 0.00 0.03325 23600.00 10000.00 0.00 0.03486 23800.00 10000.00 0.00 0.03603 24000.00 10000.00 0.00 0.03662 24200.00 10000.00 0.00 0.03693 24400.00 10000.00 0.00 0.03715 24600.00 10000.00 0.00 0.03724 24400.00 10200.00 0.00 0.03732 24600.00 10200.00 0.00 0.03740 24400.00 10400.00 0.00 0.03762 24600.00 10400.00 0.00 0.03769 24200.00 10600.00 0.00 0.03776 24400.00 10600.00 0.00 0.03804 24600.00 10600.00 0.00 0.03808 24400.00 10800.00 0.00 0.03836 24600.00 10800.00 0.00 0.03838 24200.00 11000.00 0.00 0.03849 24400.00 11000.00 0.00 0.03875 24600.00 11000.00 0.00 0.03876 24200.00 11200.00 0.00 0.03886 24400.00 11200.00 0.00 0.03908 24200.00 11400.00 0.00 0.03925 24400.00 11400.00 0.00 0.03945 24200.00 11600.00 0.00 0.03974 24400.00 11600.00 0.00 0.03993 24200.00 11800.00 0.00 0.04018 24400.00 11800.00 0.00 0.04037 24200.00 12000.00 0.00 0.04068 24400.00 12000.00 0.00 0.04087 24000.00 12200.00 0.00 0.04097 24200.00 12200.00 0.00 0.04130 24400.00 12200.00 0.00 0.04149 24000.00 12400.00 0.00 0.04161 24200.00 12400.00 0.00 0.04195 24400.00 12400.00 0.00 0.04214 24000.00 12600.00 0.00 0.04218 24200.00 12600.00 0.00 0.04253 24400.00 12600.00 0.00 0.04273 24200.00 12800.00 0.00 0.04308 24400.00 12800.00 0.00 0.04328 24200.00 13000.00 0.00 0.04364 24400.00 13000.00 0.00 0.04382 24000.00 13200.00 0.00 0.04394 24200.00 13200.00 0.00 0.04430 24400.00 13200.00 0.00 0.04445 24000.00 13400.00 0.00 0.04462 24200.00 13400.00 0.00 0.04495 24400.00 13400.00 0.00 0.04510 24000.00 13600.00 0.00 0.04523 24200.00 13600.00 0.00 0.04554 24400.00 13600.00 0.00 0.04568 24000.00 13800.00 0.00 0.04584 24200.00 13800.00 0.00 0.04615 24400.00 13800.00 0.00 0.04629 24000.00 14000.00 0.00 0.04652 24200.00 14000.00 0.00 0.04683 24400.00 14000.00 0.00 0.04698 24800.00 14000.00 0.00 0.04699 25000.00 14000.00 0.00 0.04703 24000.00 14200.00 0.00 0.04723 24200.00 14200.00 0.00 0.04756 24400.00 14200.00 0.00 0.04774 24800.00 14200.00 0.00 0.04784 25000.00 14200.00 0.00 0.04788 24000.00 14400.00 0.00 0.04796 24200.00 14400.00 0.00 0.04835 24400.00 14400.00 0.00 0.04859 24600.00 14400.00 0.00 0.04864 24800.00 14400.00 0.00 0.04876 24000.00 14600.00 0.00 0.04891 24200.00 14600.00 0.00 0.04938 24400.00 14600.00 0.00 0.04964 24600.00 14600.00 0.00 0.04968 24800.00 14600.00 0.00 0.04978 24000.00 14800.00 0.00 0.04993 24200.00 14800.00 0.00 0.05039 24400.00 14800.00 0.00 0.05060 24600.00 14800.00 0.00 0.05062 24800.00 14800.00 0.00 0.05070 24000.00 15000.00 0.00 0.05101 24200.00 15000.00 0.00 0.05140 24400.00 15000.00 0.00 0.05159 24600.00 15000.00 0.00 0.05163 24800.00 15000.00 0.00 0.05172 24000.00 15200.00 0.00 0.05215 24200.00 15200.00 0.00 0.05250 24400.00 15200.00 0.00 0.05272 24600.00 15200.00 0.00 0.05280 24800.00 15200.00 0.00 0.05287 24000.00 15400.00 0.00 0.05320 24200.00 15400.00 0.00 0.05357 24400.00 15400.00 0.00 0.05382 24600.00 15400.00 0.00 0.05388 24000.00 15600.00 0.00 0.05424 24200.00 15600.00 0.00 0.05462 24400.00 15600.00 0.00 0.05487 24600.00 15600.00 0.00 0.05489 24000.00 15800.00 0.00 0.05529 24200.00 15800.00 0.00 0.05567 24400.00 15800.00 0.00 0.05592 24600.00 15800.00 0.00 0.05592 24000.00 16000.00 0.00 0.05643 24200.00 16000.00 0.00 0.05683 24400.00 16000.00 0.00 0.05709 24000.00 16200.00 0.00 0.05757 24200.00 16200.00 0.00 0.05798 24400.00 16200.00 0.00 0.05820 24000.00 16400.00 0.00 0.05880 24200.00 16400.00 0.00 0.05918 24400.00 16400.00 0.00 0.05935 23800.00 16600.00 0.00 0.05940 24000.00 16600.00 0.00 0.06017 24200.00 16600.00 0.00 0.06052 24400.00 16600.00 0.00 0.06064 23800.00 16800.00 0.00 0.06086 24000.00 16800.00 0.00 0.06161 24200.00 16800.00 0.00 0.06191 24400.00 16800.00 0.00 0.06200 23800.00 17000.00 0.00 0.06230 24000.00 17000.00 0.00 0.06303 24200.00 17000.00 0.00 0.06330 24400.00 17000.00 0.00 0.06336 23800.00 17200.00 0.00 0.06362 24000.00 17200.00 0.00 0.06431 24200.00 17200.00 0.00 0.06453 24400.00 17200.00 0.00 0.06456 23800.00 17400.00 0.00 0.06486 24000.00 17400.00 0.00 0.06550 24200.00 17400.00 0.00 0.06569 23800.00 17600.00 0.00 0.06606 24000.00 17600.00 0.00 0.06668 24200.00 17600.00 0.00 0.06686 24400.00 17600.00 0.00 0.06686 23800.00 17800.00 0.00 0.06734 24000.00 17800.00 0.00 0.06797 24200.00 17800.00 0.00 0.06817 24400.00 17800.00 0.00 0.06819 23800.00 18000.00 0.00 0.06875 24000.00 18000.00 0.00 0.06942 24200.00 18000.00 0.00 0.06965 24400.00 18000.00 0.00 0.06967 23800.00 18200.00 0.00 0.07037 24000.00 18200.00 0.00 0.07109 24200.00 18200.00 0.00 0.07131 23800.00 18400.00 0.00 0.07197 24000.00 18400.00 0.00 0.07268 24200.00 18400.00 0.00 0.07286 23800.00 18600.00 0.00 0.07354 24000.00 18600.00 0.00 0.07421 24200.00 18600.00 0.00 0.07434 23800.00 18800.00 0.00 0.07523 24000.00 18800.00 0.00 0.07584 24200.00 18800.00 0.00 0.07592 23800.00 19000.00 0.00 0.07686 24000.00 19000.00 0.00 0.07739 24200.00 19000.00 0.00 0.07742 23800.00 19200.00 0.00 0.07852 24000.00 19200.00 0.00 0.07895 23800.00 19400.00 0.00 0.07999 24000.00 19400.00 0.00 0.08038 23800.00 19600.00 0.00 0.08147 24000.00 19600.00 0.00 0.08186 24200.00 19600.00 0.00 0.08187 23800.00 19800.00 0.00 0.08298 24000.00 19800.00 0.00 0.08337 23800.00 20000.00 0.00 0.08457 24000.00 20000.00 0.00 0.08498 23600.00 20200.00 0.00 0.08504 23800.00 20200.00 0.00 0.08630 24000.00 20200.00 0.00 0.08674 23800.00 20400.00 0.00 0.08791 24000.00 20400.00 0.00 0.08831 23600.00 20600.00 0.00 0.08843 23800.00 20600.00 0.00 0.08970 24000.00 20600.00 0.00 0.09005 23600.00 20800.00 0.00 0.09021 23800.00 20800.00 0.00 0.09149 24000.00 20800.00 0.00 0.09180 23600.00 21000.00 0.00 0.09200 23800.00 21000.00 0.00 0.09330 24000.00 21000.00 0.00 0.09355 23600.00 21200.00 0.00 0.09392 23800.00 21200.00 0.00 0.09523 24000.00 21200.00 0.00 0.09537 23600.00 21400.00 0.00 0.09599 23800.00 21400.00 0.00 0.09724 24000.00 21400.00 0.00 0.09727 23600.00 21600.00 0.00 0.09806 23800.00 21600.00 0.00 0.09927 23600.00 21800.00 0.00 0.10014 23800.00 21800.00 0.00 0.10136 23600.00 22000.00 0.00 0.10252 23800.00 22000.00 0.00 0.10374 23600.00 22200.00 0.00 0.10493 23800.00 22200.00 0.00 0.10612 23600.00 22400.00 0.00 0.10755 23800.00 22400.00 0.00 0.10867 23600.00 22600.00 0.00 0.11025 23800.00 22600.00 0.00 0.11123 23600.00 22800.00 0.00 0.11307 23800.00 22800.00 0.00 0.11387 23600.00 23000.00 0.00 0.11588 23800.00 23000.00 0.00 0.11648 23400.00 23200.00 0.00 0.11676 23600.00 23200.00 0.00 0.11867 23800.00 23200.00 0.00 0.11901 23400.00 23400.00 0.00 0.11988 23600.00 23400.00 0.00 0.12139 23600.00 23600.00 0.00 0.12237 23400.00 23800.00 35.00 0.12247 23400.00 23800.00 40.00 0.12262 23400.00 23800.00 45.00 0.12273 23400.00 23800.00 50.00 0.12277 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23463.02 23719.72 51.13 0.12302 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/253.ctf NX, NY, NZ: 512 512 1 MODE : real