CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/252.mrc Output diagnostic file name Micrographs_0601/252.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/252.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2276.000 8936.000 Mean, RMS : 4514.070 224.2903 TITLE 1: emi2mrc: 252.emi Sun Jan 05 20:02:13 2014 TITLE 2: kV 300, Mag 78000x, Def 27570, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 35.916, Y 518.848, Z -81.545, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01295 10200.00 10000.00 0.00 -0.01289 10400.00 10000.00 0.00 -0.01281 11000.00 10000.00 0.00 -0.01276 11200.00 10000.00 0.00 -0.01272 14800.00 10000.00 0.00 -0.01263 15000.00 10000.00 0.00 -0.01248 15200.00 10000.00 0.00 -0.01222 15400.00 10000.00 0.00 -0.01183 15600.00 10000.00 0.00 -0.01130 15800.00 10000.00 0.00 -0.01066 16000.00 10000.00 0.00 -0.01010 16200.00 10000.00 0.00 -0.00949 16400.00 10000.00 0.00 -0.00888 16600.00 10000.00 0.00 -0.00848 16800.00 10000.00 0.00 -0.00797 17000.00 10000.00 0.00 -0.00732 17200.00 10000.00 0.00 -0.00674 17400.00 10000.00 0.00 -0.00610 17600.00 10000.00 0.00 -0.00542 17800.00 10000.00 0.00 -0.00472 18000.00 10000.00 0.00 -0.00395 18200.00 10000.00 0.00 -0.00306 18400.00 10000.00 0.00 -0.00216 18600.00 10000.00 0.00 -0.00117 18800.00 10000.00 0.00 -0.00010 19000.00 10000.00 0.00 0.00088 19200.00 10000.00 0.00 0.00176 19400.00 10000.00 0.00 0.00258 19600.00 10000.00 0.00 0.00344 19800.00 10000.00 0.00 0.00434 20000.00 10000.00 0.00 0.00538 20200.00 10000.00 0.00 0.00676 20400.00 10000.00 0.00 0.00814 20600.00 10000.00 0.00 0.00928 20800.00 10000.00 0.00 0.01031 21000.00 10000.00 0.00 0.01140 21200.00 10000.00 0.00 0.01269 21400.00 10000.00 0.00 0.01402 21600.00 10000.00 0.00 0.01519 21800.00 10000.00 0.00 0.01632 22000.00 10000.00 0.00 0.01744 22200.00 10000.00 0.00 0.01867 22400.00 10000.00 0.00 0.01995 22600.00 10000.00 0.00 0.02115 22800.00 10000.00 0.00 0.02254 23000.00 10000.00 0.00 0.02420 23200.00 10000.00 0.00 0.02609 23400.00 10000.00 0.00 0.02814 23600.00 10000.00 0.00 0.03024 23800.00 10000.00 0.00 0.03250 24000.00 10000.00 0.00 0.03424 24200.00 10000.00 0.00 0.03511 24400.00 10000.00 0.00 0.03540 24600.00 10000.00 0.00 0.03542 24400.00 10200.00 0.00 0.03563 24600.00 10200.00 0.00 0.03564 24800.00 10200.00 0.00 0.03565 25200.00 10200.00 0.00 0.03568 24400.00 10400.00 0.00 0.03587 24600.00 10400.00 0.00 0.03592 24800.00 10400.00 0.00 0.03599 24400.00 10600.00 0.00 0.03625 24600.00 10600.00 0.00 0.03630 24800.00 10600.00 0.00 0.03636 24200.00 10800.00 0.00 0.03638 24400.00 10800.00 0.00 0.03661 24600.00 10800.00 0.00 0.03664 24800.00 10800.00 0.00 0.03669 24200.00 11000.00 0.00 0.03679 24400.00 11000.00 0.00 0.03699 24600.00 11000.00 0.00 0.03705 24800.00 11000.00 0.00 0.03714 24200.00 11200.00 0.00 0.03721 24400.00 11200.00 0.00 0.03746 24600.00 11200.00 0.00 0.03759 24800.00 11200.00 0.00 0.03771 24400.00 11400.00 0.00 0.03794 24600.00 11400.00 0.00 0.03810 24800.00 11400.00 0.00 0.03818 24400.00 11600.00 0.00 0.03830 24600.00 11600.00 0.00 0.03843 24800.00 11600.00 0.00 0.03843 24400.00 11800.00 0.00 0.03872 24600.00 11800.00 0.00 0.03881 24200.00 12000.00 0.00 0.03895 24400.00 12000.00 0.00 0.03927 24600.00 12000.00 0.00 0.03935 24200.00 12200.00 0.00 0.03957 24400.00 12200.00 0.00 0.03987 24600.00 12200.00 0.00 0.03991 24200.00 12400.00 0.00 0.04015 24400.00 12400.00 0.00 0.04042 24200.00 12600.00 0.00 0.04066 24400.00 12600.00 0.00 0.04090 24200.00 12800.00 0.00 0.04120 24400.00 12800.00 0.00 0.04140 24200.00 13000.00 0.00 0.04181 24400.00 13000.00 0.00 0.04196 24200.00 13200.00 0.00 0.04254 24400.00 13200.00 0.00 0.04264 24200.00 13400.00 0.00 0.04326 24400.00 13400.00 0.00 0.04338 24200.00 13600.00 0.00 0.04401 24400.00 13600.00 0.00 0.04418 24200.00 13800.00 0.00 0.04484 24400.00 13800.00 0.00 0.04502 24200.00 14000.00 0.00 0.04563 24400.00 14000.00 0.00 0.04581 24200.00 14200.00 0.00 0.04645 24400.00 14200.00 0.00 0.04666 24200.00 14400.00 0.00 0.04736 24400.00 14400.00 0.00 0.04759 24600.00 14400.00 0.00 0.04760 24200.00 14600.00 0.00 0.04822 24400.00 14600.00 0.00 0.04843 24600.00 14600.00 0.00 0.04844 24200.00 14800.00 0.00 0.04904 24400.00 14800.00 0.00 0.04925 24600.00 14800.00 0.00 0.04927 24200.00 15000.00 0.00 0.05000 24400.00 15000.00 0.00 0.05024 24600.00 15000.00 0.00 0.05024 24200.00 15200.00 0.00 0.05088 24400.00 15200.00 0.00 0.05111 24200.00 15400.00 0.00 0.05175 24400.00 15400.00 0.00 0.05196 24200.00 15600.00 0.00 0.05272 24400.00 15600.00 0.00 0.05292 24200.00 15800.00 0.00 0.05370 24400.00 15800.00 0.00 0.05387 24200.00 16000.00 0.00 0.05478 24400.00 16000.00 0.00 0.05493 24200.00 16200.00 0.00 0.05588 24400.00 16200.00 0.00 0.05601 24000.00 16400.00 0.00 0.05609 24200.00 16400.00 0.00 0.05710 24400.00 16400.00 0.00 0.05717 24000.00 16600.00 0.00 0.05737 24200.00 16600.00 0.00 0.05832 24400.00 16600.00 0.00 0.05833 24000.00 16800.00 0.00 0.05870 24200.00 16800.00 0.00 0.05958 24000.00 17000.00 0.00 0.06005 24200.00 17000.00 0.00 0.06088 24000.00 17200.00 0.00 0.06128 24200.00 17200.00 0.00 0.06210 24000.00 17400.00 0.00 0.06257 24200.00 17400.00 0.00 0.06342 24400.00 17400.00 0.00 0.06345 24000.00 17600.00 0.00 0.06398 24200.00 17600.00 0.00 0.06485 24400.00 17600.00 0.00 0.06487 24000.00 17800.00 0.00 0.06549 24200.00 17800.00 0.00 0.06635 24400.00 17800.00 0.00 0.06636 24000.00 18000.00 0.00 0.06686 24200.00 18000.00 0.00 0.06772 24000.00 18200.00 0.00 0.06819 24200.00 18200.00 0.00 0.06905 24000.00 18400.00 0.00 0.06967 24200.00 18400.00 0.00 0.07055 24000.00 18600.00 0.00 0.07128 24200.00 18600.00 0.00 0.07216 24000.00 18800.00 0.00 0.07293 24200.00 18800.00 0.00 0.07377 24000.00 19000.00 0.00 0.07461 24200.00 19000.00 0.00 0.07536 24000.00 19200.00 0.00 0.07623 24200.00 19200.00 0.00 0.07691 24000.00 19400.00 0.00 0.07774 24200.00 19400.00 0.00 0.07841 24000.00 19600.00 0.00 0.07945 24200.00 19600.00 0.00 0.08013 24000.00 19800.00 0.00 0.08108 24200.00 19800.00 0.00 0.08176 24000.00 20000.00 0.00 0.08271 24200.00 20000.00 0.00 0.08336 24000.00 20200.00 0.00 0.08449 24200.00 20200.00 0.00 0.08510 24000.00 20400.00 0.00 0.08635 24200.00 20400.00 0.00 0.08691 24000.00 20600.00 0.00 0.08834 24200.00 20600.00 0.00 0.08881 24000.00 20800.00 0.00 0.09019 24200.00 20800.00 0.00 0.09054 24000.00 21000.00 0.00 0.09215 24200.00 21000.00 0.00 0.09242 24000.00 21200.00 0.00 0.09409 24200.00 21200.00 0.00 0.09430 24000.00 21400.00 0.00 0.09614 24200.00 21400.00 0.00 0.09630 24000.00 21600.00 0.00 0.09829 24200.00 21600.00 0.00 0.09839 24000.00 21800.00 0.00 0.10052 24200.00 21800.00 0.00 0.10055 23800.00 22000.00 0.00 0.10085 24000.00 22000.00 0.00 0.10302 23800.00 22200.00 0.00 0.10324 24000.00 22200.00 0.00 0.10532 23800.00 22400.00 0.00 0.10577 24000.00 22400.00 0.00 0.10779 23800.00 22600.00 0.00 0.10830 24000.00 22600.00 0.00 0.11026 23800.00 22800.00 0.00 0.11113 24000.00 22800.00 0.00 0.11304 23800.00 23000.00 0.00 0.11416 24000.00 23000.00 0.00 0.11597 23800.00 23200.00 0.00 0.11728 24000.00 23200.00 0.00 0.11894 23800.00 23400.00 0.00 0.12064 24000.00 23400.00 0.00 0.12204 23800.00 23600.00 0.00 0.12372 24000.00 23600.00 0.00 0.12466 23800.00 23800.00 0.00 0.12676 24000.00 23800.00 0.00 0.12677 23800.00 24000.00 0.00 0.12755 23800.00 24000.00 5.00 0.12777 23800.00 24000.00 10.00 0.12797 23800.00 24000.00 15.00 0.12815 23800.00 24000.00 20.00 0.12831 23800.00 24000.00 25.00 0.12843 23800.00 24000.00 30.00 0.12851 23800.00 24000.00 35.00 0.12854 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23801.43 23987.70 36.68 0.12856 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/252.ctf NX, NY, NZ: 512 512 1 MODE : real