CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/251.mrc Output diagnostic file name Micrographs_0601/251.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/251.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2422.000 8200.000 Mean, RMS : 4508.178 225.4287 TITLE 1: emi2mrc: 251.emi Sun Jan 05 20:04:01 2014 TITLE 2: kV 300, Mag 78000x, Def 27570, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 33.328, Y 519.141, Z -81.545, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01445 11400.00 10000.00 0.00 -0.01439 11600.00 10000.00 0.00 -0.01426 11800.00 10000.00 0.00 -0.01418 12000.00 10000.00 0.00 -0.01415 12200.00 10000.00 0.00 -0.01413 12400.00 10000.00 0.00 -0.01406 12600.00 10000.00 0.00 -0.01384 12800.00 10000.00 0.00 -0.01365 13000.00 10000.00 0.00 -0.01350 13200.00 10000.00 0.00 -0.01319 13400.00 10000.00 0.00 -0.01289 13600.00 10000.00 0.00 -0.01265 13800.00 10000.00 0.00 -0.01254 14200.00 10000.00 0.00 -0.01241 14400.00 10000.00 0.00 -0.01209 14600.00 10000.00 0.00 -0.01179 14800.00 10000.00 0.00 -0.01160 15000.00 10000.00 0.00 -0.01153 15200.00 10000.00 0.00 -0.01126 15400.00 10000.00 0.00 -0.01079 15600.00 10000.00 0.00 -0.01053 15800.00 10000.00 0.00 -0.01018 16000.00 10000.00 0.00 -0.00974 16200.00 10000.00 0.00 -0.00933 16400.00 10000.00 0.00 -0.00876 16600.00 10000.00 0.00 -0.00817 16800.00 10000.00 0.00 -0.00747 17000.00 10000.00 0.00 -0.00666 17200.00 10000.00 0.00 -0.00589 17400.00 10000.00 0.00 -0.00513 17600.00 10000.00 0.00 -0.00441 17800.00 10000.00 0.00 -0.00375 18000.00 10000.00 0.00 -0.00301 18200.00 10000.00 0.00 -0.00217 18400.00 10000.00 0.00 -0.00124 18600.00 10000.00 0.00 -0.00022 18800.00 10000.00 0.00 0.00074 19000.00 10000.00 0.00 0.00167 19200.00 10000.00 0.00 0.00259 19400.00 10000.00 0.00 0.00342 19600.00 10000.00 0.00 0.00434 19800.00 10000.00 0.00 0.00519 20000.00 10000.00 0.00 0.00584 20200.00 10000.00 0.00 0.00652 20400.00 10000.00 0.00 0.00725 20600.00 10000.00 0.00 0.00803 20800.00 10000.00 0.00 0.00890 21000.00 10000.00 0.00 0.00986 21200.00 10000.00 0.00 0.01099 21400.00 10000.00 0.00 0.01219 21600.00 10000.00 0.00 0.01348 21800.00 10000.00 0.00 0.01499 22000.00 10000.00 0.00 0.01655 22200.00 10000.00 0.00 0.01813 22400.00 10000.00 0.00 0.01977 22600.00 10000.00 0.00 0.02140 22800.00 10000.00 0.00 0.02305 23000.00 10000.00 0.00 0.02473 23200.00 10000.00 0.00 0.02647 23400.00 10000.00 0.00 0.02827 23600.00 10000.00 0.00 0.03008 23800.00 10000.00 0.00 0.03208 24000.00 10000.00 0.00 0.03395 24200.00 10000.00 0.00 0.03506 24400.00 10000.00 0.00 0.03552 24600.00 10000.00 0.00 0.03569 24800.00 10000.00 0.00 0.03571 24400.00 10200.00 0.00 0.03575 24600.00 10200.00 0.00 0.03588 24400.00 10400.00 0.00 0.03611 24600.00 10400.00 0.00 0.03620 24400.00 10600.00 0.00 0.03644 24600.00 10600.00 0.00 0.03653 24800.00 10600.00 0.00 0.03659 24400.00 10800.00 0.00 0.03684 24600.00 10800.00 0.00 0.03700 24800.00 10800.00 0.00 0.03716 24400.00 11000.00 0.00 0.03726 24600.00 11000.00 0.00 0.03748 24800.00 11000.00 0.00 0.03764 24600.00 11200.00 0.00 0.03784 24800.00 11200.00 0.00 0.03798 24400.00 11400.00 0.00 0.03804 24600.00 11400.00 0.00 0.03832 24800.00 11400.00 0.00 0.03844 24600.00 11600.00 0.00 0.03872 24800.00 11600.00 0.00 0.03879 24400.00 11800.00 0.00 0.03885 24600.00 11800.00 0.00 0.03912 24800.00 11800.00 0.00 0.03912 24400.00 12000.00 0.00 0.03935 24600.00 12000.00 0.00 0.03957 24400.00 12200.00 0.00 0.03994 24600.00 12200.00 0.00 0.04013 24800.00 12200.00 0.00 0.04017 25000.00 12200.00 0.00 0.04017 24400.00 12400.00 0.00 0.04057 24600.00 12400.00 0.00 0.04078 24800.00 12400.00 0.00 0.04087 25000.00 12400.00 0.00 0.04091 24400.00 12600.00 0.00 0.04110 24600.00 12600.00 0.00 0.04135 24800.00 12600.00 0.00 0.04148 25000.00 12600.00 0.00 0.04150 24400.00 12800.00 0.00 0.04174 24600.00 12800.00 0.00 0.04200 24800.00 12800.00 0.00 0.04212 24400.00 13000.00 0.00 0.04240 24600.00 13000.00 0.00 0.04261 24800.00 13000.00 0.00 0.04270 24400.00 13200.00 0.00 0.04296 24600.00 13200.00 0.00 0.04312 24800.00 13200.00 0.00 0.04320 24400.00 13400.00 0.00 0.04347 24600.00 13400.00 0.00 0.04361 24800.00 13400.00 0.00 0.04371 24400.00 13600.00 0.00 0.04415 24600.00 13600.00 0.00 0.04430 24800.00 13600.00 0.00 0.04442 24400.00 13800.00 0.00 0.04495 24600.00 13800.00 0.00 0.04512 24800.00 13800.00 0.00 0.04524 24200.00 14000.00 0.00 0.04529 24400.00 14000.00 0.00 0.04583 24600.00 14000.00 0.00 0.04598 24800.00 14000.00 0.00 0.04604 24200.00 14200.00 0.00 0.04620 24400.00 14200.00 0.00 0.04668 24600.00 14200.00 0.00 0.04677 24800.00 14200.00 0.00 0.04678 24200.00 14400.00 0.00 0.04686 24400.00 14400.00 0.00 0.04727 24600.00 14400.00 0.00 0.04734 24800.00 14400.00 0.00 0.04740 24200.00 14600.00 0.00 0.04766 24400.00 14600.00 0.00 0.04806 24600.00 14600.00 0.00 0.04820 24800.00 14600.00 0.00 0.04837 24200.00 14800.00 0.00 0.04861 24400.00 14800.00 0.00 0.04904 24600.00 14800.00 0.00 0.04926 24800.00 14800.00 0.00 0.04942 24200.00 15000.00 0.00 0.04956 24400.00 15000.00 0.00 0.05001 24600.00 15000.00 0.00 0.05025 24800.00 15000.00 0.00 0.05037 24200.00 15200.00 0.00 0.05061 24400.00 15200.00 0.00 0.05107 24600.00 15200.00 0.00 0.05131 24800.00 15200.00 0.00 0.05138 24200.00 15400.00 0.00 0.05171 24400.00 15400.00 0.00 0.05218 24600.00 15400.00 0.00 0.05241 24800.00 15400.00 0.00 0.05246 24200.00 15600.00 0.00 0.05280 24400.00 15600.00 0.00 0.05329 24600.00 15600.00 0.00 0.05352 24800.00 15600.00 0.00 0.05357 24200.00 15800.00 0.00 0.05394 24400.00 15800.00 0.00 0.05444 24600.00 15800.00 0.00 0.05466 24800.00 15800.00 0.00 0.05470 24200.00 16000.00 0.00 0.05523 24400.00 16000.00 0.00 0.05570 24600.00 16000.00 0.00 0.05590 24800.00 16000.00 0.00 0.05591 24200.00 16200.00 0.00 0.05630 24400.00 16200.00 0.00 0.05675 24600.00 16200.00 0.00 0.05694 24200.00 16400.00 0.00 0.05726 24400.00 16400.00 0.00 0.05772 24600.00 16400.00 0.00 0.05791 24200.00 16600.00 0.00 0.05829 24400.00 16600.00 0.00 0.05877 24600.00 16600.00 0.00 0.05896 24200.00 16800.00 0.00 0.05939 24400.00 16800.00 0.00 0.05990 24600.00 16800.00 0.00 0.06010 24200.00 17000.00 0.00 0.06057 24400.00 17000.00 0.00 0.06114 24600.00 17000.00 0.00 0.06135 24200.00 17200.00 0.00 0.06188 24400.00 17200.00 0.00 0.06247 24600.00 17200.00 0.00 0.06266 24200.00 17400.00 0.00 0.06337 24400.00 17400.00 0.00 0.06396 24600.00 17400.00 0.00 0.06413 24200.00 17600.00 0.00 0.06476 24400.00 17600.00 0.00 0.06535 24600.00 17600.00 0.00 0.06550 24200.00 17800.00 0.00 0.06615 24400.00 17800.00 0.00 0.06673 24600.00 17800.00 0.00 0.06683 24200.00 18000.00 0.00 0.06756 24400.00 18000.00 0.00 0.06811 24600.00 18000.00 0.00 0.06814 24200.00 18200.00 0.00 0.06900 24400.00 18200.00 0.00 0.06953 24600.00 18200.00 0.00 0.06955 24200.00 18400.00 0.00 0.07055 24400.00 18400.00 0.00 0.07108 24600.00 18400.00 0.00 0.07109 24200.00 18600.00 0.00 0.07203 24400.00 18600.00 0.00 0.07251 24200.00 18800.00 0.00 0.07347 24400.00 18800.00 0.00 0.07394 24200.00 19000.00 0.00 0.07499 24400.00 19000.00 0.00 0.07547 24200.00 19200.00 0.00 0.07661 24400.00 19200.00 0.00 0.07706 24200.00 19400.00 0.00 0.07825 24400.00 19400.00 0.00 0.07863 24200.00 19600.00 0.00 0.07996 24400.00 19600.00 0.00 0.08030 24200.00 19800.00 0.00 0.08162 24400.00 19800.00 0.00 0.08193 24000.00 20000.00 0.00 0.08196 24200.00 20000.00 0.00 0.08335 24400.00 20000.00 0.00 0.08360 24000.00 20200.00 0.00 0.08367 24200.00 20200.00 0.00 0.08506 24400.00 20200.00 0.00 0.08524 24000.00 20400.00 0.00 0.08527 24200.00 20400.00 0.00 0.08667 24400.00 20400.00 0.00 0.08681 24000.00 20600.00 0.00 0.08709 24200.00 20600.00 0.00 0.08848 24400.00 20600.00 0.00 0.08855 24000.00 20800.00 0.00 0.08896 24200.00 20800.00 0.00 0.09030 24000.00 21000.00 0.00 0.09088 24200.00 21000.00 0.00 0.09215 24000.00 21200.00 0.00 0.09270 24200.00 21200.00 0.00 0.09394 24000.00 21400.00 0.00 0.09467 24200.00 21400.00 0.00 0.09592 24000.00 21600.00 0.00 0.09693 24200.00 21600.00 0.00 0.09818 24000.00 21800.00 0.00 0.09939 24200.00 21800.00 0.00 0.10061 24000.00 22000.00 0.00 0.10195 24200.00 22000.00 0.00 0.10308 24000.00 22200.00 0.00 0.10440 24200.00 22200.00 0.00 0.10543 24000.00 22400.00 0.00 0.10708 24200.00 22400.00 0.00 0.10798 24000.00 22600.00 0.00 0.10975 24200.00 22600.00 0.00 0.11050 24000.00 22800.00 0.00 0.11262 24200.00 22800.00 0.00 0.11319 24000.00 23000.00 0.00 0.11562 24200.00 23000.00 0.00 0.11600 24000.00 23200.00 0.00 0.11885 24200.00 23200.00 0.00 0.11895 23800.00 23400.00 0.00 0.11952 24000.00 23400.00 0.00 0.12225 23800.00 23600.00 0.00 0.12295 24000.00 23600.00 0.00 0.12543 23800.00 23800.00 0.00 0.12666 24000.00 23800.00 0.00 0.12844 24000.00 24000.00 0.00 0.12889 23800.00 24000.00 15.00 0.12890 23800.00 24000.00 20.00 0.12910 23800.00 24000.00 25.00 0.12927 23800.00 24000.00 30.00 0.12942 23800.00 24000.00 35.00 0.12953 23800.00 24000.00 40.00 0.12960 23800.00 24000.00 45.00 0.12964 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23849.18 24064.97 45.00 0.13009 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/251.ctf NX, NY, NZ: 512 512 1 MODE : real