CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/250.mrc Output diagnostic file name Micrographs_0601/250.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/250.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2390.000 7740.000 Mean, RMS : 4528.918 226.7910 TITLE 1: emi2mrc: 250.emi Sun Jan 05 20:01:20 2014 TITLE 2: kV 300, Mag 78000x, Def 27730, Spot 4, Int 55.08%, Obj 88.04%, Dif 73.67% TITLE 3: X 38.919, Y 518.588, Z -81.545, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02110 19400.00 10000.00 0.00 -0.02097 19600.00 10000.00 0.00 -0.02049 19800.00 10000.00 0.00 -0.01999 20000.00 10000.00 0.00 -0.01957 20200.00 10000.00 0.00 -0.01932 20400.00 10000.00 0.00 -0.01902 20600.00 10000.00 0.00 -0.01863 20800.00 10000.00 0.00 -0.01805 21000.00 10000.00 0.00 -0.01725 21200.00 10000.00 0.00 -0.01643 21400.00 10000.00 0.00 -0.01565 21600.00 10000.00 0.00 -0.01488 21800.00 10000.00 0.00 -0.01413 22000.00 10000.00 0.00 -0.01341 22200.00 10000.00 0.00 -0.01272 22400.00 10000.00 0.00 -0.01206 22600.00 10000.00 0.00 -0.01153 22800.00 10000.00 0.00 -0.01100 23000.00 10000.00 0.00 -0.01036 23200.00 10000.00 0.00 -0.00968 23400.00 10000.00 0.00 -0.00902 23600.00 10000.00 0.00 -0.00842 23800.00 10000.00 0.00 -0.00768 24000.00 10000.00 0.00 -0.00696 24200.00 10000.00 0.00 -0.00647 24400.00 10000.00 0.00 -0.00594 24600.00 10000.00 0.00 -0.00543 24800.00 10000.00 0.00 -0.00490 25000.00 10000.00 0.00 -0.00422 25200.00 10000.00 0.00 -0.00346 25400.00 10000.00 0.00 -0.00260 25600.00 10000.00 0.00 -0.00178 25800.00 10000.00 0.00 -0.00110 26000.00 10000.00 0.00 -0.00047 26200.00 10000.00 0.00 0.00013 26400.00 10000.00 0.00 0.00087 26600.00 10000.00 0.00 0.00194 26800.00 10000.00 0.00 0.00310 27000.00 10000.00 0.00 0.00435 27200.00 10000.00 0.00 0.00571 27400.00 10000.00 0.00 0.00702 27600.00 10000.00 0.00 0.00843 27800.00 10000.00 0.00 0.00985 28000.00 10000.00 0.00 0.01132 28200.00 10000.00 0.00 0.01299 28400.00 10000.00 0.00 0.01475 28600.00 10000.00 0.00 0.01618 28800.00 10000.00 0.00 0.01688 29000.00 10000.00 0.00 0.01719 29200.00 10000.00 0.00 0.01735 29200.00 10200.00 0.00 0.01743 29000.00 10400.00 0.00 0.01745 29200.00 10400.00 0.00 0.01765 29400.00 10400.00 0.00 0.01765 29000.00 10600.00 0.00 0.01782 29200.00 10600.00 0.00 0.01804 29000.00 10800.00 0.00 0.01817 29200.00 10800.00 0.00 0.01838 29000.00 11000.00 0.00 0.01852 29200.00 11000.00 0.00 0.01871 29000.00 11200.00 0.00 0.01880 29200.00 11200.00 0.00 0.01897 29000.00 11400.00 0.00 0.01897 29200.00 11400.00 0.00 0.01912 29200.00 11600.00 0.00 0.01923 29000.00 11800.00 0.00 0.01924 29200.00 11800.00 0.00 0.01933 29000.00 12000.00 0.00 0.01933 29200.00 12000.00 0.00 0.01939 29000.00 12200.00 0.00 0.01939 29200.00 12200.00 0.00 0.01944 29000.00 12400.00 0.00 0.01946 29200.00 12400.00 0.00 0.01947 29200.00 13000.00 0.00 0.01953 29000.00 13200.00 0.00 0.01956 29200.00 13200.00 0.00 0.01959 29000.00 13400.00 0.00 0.01964 29000.00 13600.00 0.00 0.01972 29000.00 13800.00 0.00 0.01992 28800.00 14000.00 0.00 0.02007 29000.00 14000.00 0.00 0.02025 29400.00 14000.00 0.00 0.02026 29600.00 14000.00 0.00 0.02027 28800.00 14200.00 0.00 0.02048 29000.00 14200.00 0.00 0.02067 29200.00 14200.00 0.00 0.02072 29400.00 14200.00 0.00 0.02079 28800.00 14400.00 0.00 0.02092 29000.00 14400.00 0.00 0.02115 29200.00 14400.00 0.00 0.02123 29400.00 14400.00 0.00 0.02128 28800.00 14600.00 0.00 0.02135 29000.00 14600.00 0.00 0.02157 29200.00 14600.00 0.00 0.02166 29400.00 14600.00 0.00 0.02167 28800.00 14800.00 0.00 0.02171 29000.00 14800.00 0.00 0.02194 29200.00 14800.00 0.00 0.02204 28800.00 15000.00 0.00 0.02206 29000.00 15000.00 0.00 0.02233 29200.00 15000.00 0.00 0.02245 29000.00 15200.00 0.00 0.02267 29200.00 15200.00 0.00 0.02279 29000.00 15400.00 0.00 0.02310 29200.00 15400.00 0.00 0.02323 28800.00 15600.00 0.00 0.02327 29000.00 15600.00 0.00 0.02359 29200.00 15600.00 0.00 0.02373 29000.00 15800.00 0.00 0.02400 29200.00 15800.00 0.00 0.02412 28800.00 16000.00 0.00 0.02415 29000.00 16000.00 0.00 0.02445 29200.00 16000.00 0.00 0.02455 28800.00 16200.00 0.00 0.02462 29000.00 16200.00 0.00 0.02490 29200.00 16200.00 0.00 0.02499 28800.00 16400.00 0.00 0.02500 29000.00 16400.00 0.00 0.02528 29200.00 16400.00 0.00 0.02536 29000.00 16600.00 0.00 0.02561 29200.00 16600.00 0.00 0.02567 28800.00 16800.00 0.00 0.02581 29000.00 16800.00 0.00 0.02612 29200.00 16800.00 0.00 0.02617 28800.00 17000.00 0.00 0.02655 29000.00 17000.00 0.00 0.02684 29200.00 17000.00 0.00 0.02688 28800.00 17200.00 0.00 0.02728 29000.00 17200.00 0.00 0.02756 29200.00 17200.00 0.00 0.02761 28800.00 17400.00 0.00 0.02800 29000.00 17400.00 0.00 0.02828 29200.00 17400.00 0.00 0.02835 28800.00 17600.00 0.00 0.02878 29000.00 17600.00 0.00 0.02906 29200.00 17600.00 0.00 0.02912 28800.00 17800.00 0.00 0.02967 29000.00 17800.00 0.00 0.02995 29200.00 17800.00 0.00 0.03000 28600.00 18000.00 0.00 0.03006 28800.00 18000.00 0.00 0.03062 29000.00 18000.00 0.00 0.03093 29200.00 18000.00 0.00 0.03099 28600.00 18200.00 0.00 0.03102 28800.00 18200.00 0.00 0.03161 29000.00 18200.00 0.00 0.03195 29200.00 18200.00 0.00 0.03198 28800.00 18400.00 0.00 0.03258 29000.00 18400.00 0.00 0.03291 28800.00 18600.00 0.00 0.03355 29000.00 18600.00 0.00 0.03386 28800.00 18800.00 0.00 0.03451 29000.00 18800.00 0.00 0.03480 28600.00 19000.00 0.00 0.03480 28800.00 19000.00 0.00 0.03551 29000.00 19000.00 0.00 0.03579 28600.00 19200.00 0.00 0.03597 28800.00 19200.00 0.00 0.03665 29000.00 19200.00 0.00 0.03690 28600.00 19400.00 0.00 0.03703 28800.00 19400.00 0.00 0.03764 29000.00 19400.00 0.00 0.03780 28600.00 19600.00 0.00 0.03791 28800.00 19600.00 0.00 0.03845 29000.00 19600.00 0.00 0.03858 28600.00 19800.00 0.00 0.03876 28800.00 19800.00 0.00 0.03931 29000.00 19800.00 0.00 0.03946 28600.00 20000.00 0.00 0.03975 28800.00 20000.00 0.00 0.04033 29000.00 20000.00 0.00 0.04051 28600.00 20200.00 0.00 0.04077 28800.00 20200.00 0.00 0.04138 29000.00 20200.00 0.00 0.04158 28600.00 20400.00 0.00 0.04177 28800.00 20400.00 0.00 0.04241 29000.00 20400.00 0.00 0.04261 28600.00 20600.00 0.00 0.04294 28800.00 20600.00 0.00 0.04359 29000.00 20600.00 0.00 0.04381 28600.00 20800.00 0.00 0.04418 28800.00 20800.00 0.00 0.04487 29000.00 20800.00 0.00 0.04506 28600.00 21000.00 0.00 0.04545 28800.00 21000.00 0.00 0.04615 29000.00 21000.00 0.00 0.04630 28600.00 21200.00 0.00 0.04655 28800.00 21200.00 0.00 0.04725 29000.00 21200.00 0.00 0.04735 28600.00 21400.00 0.00 0.04767 28800.00 21400.00 0.00 0.04835 29000.00 21400.00 0.00 0.04845 28600.00 21600.00 0.00 0.04890 28800.00 21600.00 0.00 0.04957 29000.00 21600.00 0.00 0.04968 28600.00 21800.00 0.00 0.05014 28800.00 21800.00 0.00 0.05083 29000.00 21800.00 0.00 0.05095 28600.00 22000.00 0.00 0.05151 28800.00 22000.00 0.00 0.05217 29000.00 22000.00 0.00 0.05227 28600.00 22200.00 0.00 0.05272 28800.00 22200.00 0.00 0.05335 29000.00 22200.00 0.00 0.05344 28600.00 22400.00 0.00 0.05394 28800.00 22400.00 0.00 0.05456 29000.00 22400.00 0.00 0.05468 28600.00 22600.00 0.00 0.05525 28800.00 22600.00 0.00 0.05587 29000.00 22600.00 0.00 0.05598 28600.00 22800.00 0.00 0.05653 28800.00 22800.00 0.00 0.05712 29000.00 22800.00 0.00 0.05720 28600.00 23000.00 0.00 0.05772 28800.00 23000.00 0.00 0.05826 29000.00 23000.00 0.00 0.05832 28600.00 23200.00 0.00 0.05885 28800.00 23200.00 0.00 0.05934 29000.00 23200.00 0.00 0.05942 28600.00 23400.00 0.00 0.06001 28800.00 23400.00 0.00 0.06054 29000.00 23400.00 0.00 0.06063 28600.00 23600.00 0.00 0.06124 28800.00 23600.00 0.00 0.06178 29000.00 23600.00 0.00 0.06184 28600.00 23800.00 0.00 0.06256 28800.00 23800.00 0.00 0.06308 28600.00 24000.00 0.00 0.06389 28800.00 24000.00 0.00 0.06436 28600.00 24200.00 0.00 0.06535 28800.00 24200.00 0.00 0.06580 28600.00 24400.00 0.00 0.06688 28800.00 24400.00 0.00 0.06730 28600.00 24600.00 0.00 0.06845 28800.00 24600.00 0.00 0.06886 28600.00 24800.00 0.00 0.07021 28800.00 24800.00 0.00 0.07062 28600.00 25000.00 0.00 0.07198 28800.00 25000.00 0.00 0.07236 28600.00 25200.00 0.00 0.07380 28800.00 25200.00 0.00 0.07413 28600.00 25400.00 0.00 0.07570 28800.00 25400.00 0.00 0.07596 28600.00 25600.00 0.00 0.07755 28800.00 25600.00 0.00 0.07771 28400.00 25800.00 0.00 0.07773 28600.00 25800.00 0.00 0.07933 28800.00 25800.00 0.00 0.07943 28400.00 26000.00 0.00 0.07971 28600.00 26000.00 0.00 0.08128 28800.00 26000.00 0.00 0.08130 28400.00 26200.00 0.00 0.08169 28600.00 26200.00 0.00 0.08322 28400.00 26400.00 0.00 0.08374 28600.00 26400.00 0.00 0.08522 28400.00 26600.00 0.00 0.08585 28600.00 26600.00 0.00 0.08724 28400.00 26800.00 0.00 0.08786 28600.00 26800.00 0.00 0.08918 28400.00 27000.00 0.00 0.09017 28600.00 27000.00 0.00 0.09147 28400.00 27200.00 0.00 0.09253 28600.00 27200.00 0.00 0.09379 28400.00 27400.00 0.00 0.09492 28600.00 27400.00 0.00 0.09609 28400.00 27600.00 0.00 0.09735 28600.00 27600.00 0.00 0.09839 28400.00 27800.00 0.00 0.09989 28600.00 27800.00 0.00 0.10072 28400.00 28000.00 0.00 0.10231 28600.00 28000.00 0.00 0.10284 28400.00 28200.00 0.00 0.10459 28600.00 28200.00 0.00 0.10469 28400.00 28400.00 0.00 0.10649 28400.00 28600.00 15.00 0.10657 28400.00 28600.00 20.00 0.10670 28400.00 28600.00 25.00 0.10681 28400.00 28600.00 30.00 0.10687 28400.00 28600.00 35.00 0.10690 28200.00 28600.00 40.00 0.10692 28200.00 28600.00 45.00 0.10698 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 28200.00 28600.00 45.00 0.10698 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/250.ctf NX, NY, NZ: 512 512 1 MODE : real