CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/248.mrc Output diagnostic file name Micrographs_0601/248.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/248.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2016.000 7464.000 Mean, RMS : 4326.979 208.9265 TITLE 1: emi2mrc: 248.emi Sun Jan 05 19:59:48 2014 TITLE 2: kV 300, Mag 78000x, Def 27730, Spot 4, Int 55.08%, Obj 88.04%, Dif 73.67% TITLE 3: X 54.424, Y 516.727, Z -81.545, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00379 10200.00 10000.00 0.00 -0.00355 10400.00 10000.00 0.00 -0.00339 10600.00 10000.00 0.00 -0.00322 10800.00 10000.00 0.00 -0.00308 11000.00 10000.00 0.00 -0.00295 11200.00 10000.00 0.00 -0.00289 11400.00 10000.00 0.00 -0.00284 11600.00 10000.00 0.00 -0.00243 11800.00 10000.00 0.00 -0.00221 12000.00 10000.00 0.00 -0.00215 12200.00 10000.00 0.00 -0.00198 12400.00 10000.00 0.00 -0.00173 12600.00 10000.00 0.00 -0.00128 12800.00 10000.00 0.00 -0.00112 13000.00 10000.00 0.00 -0.00095 13200.00 10000.00 0.00 -0.00043 13400.00 10000.00 0.00 0.00009 13600.00 10000.00 0.00 0.00075 13800.00 10000.00 0.00 0.00149 14000.00 10000.00 0.00 0.00219 14200.00 10000.00 0.00 0.00303 14400.00 10000.00 0.00 0.00387 14600.00 10000.00 0.00 0.00443 14800.00 10000.00 0.00 0.00501 15000.00 10000.00 0.00 0.00585 15200.00 10000.00 0.00 0.00679 15400.00 10000.00 0.00 0.00777 15600.00 10000.00 0.00 0.00865 15800.00 10000.00 0.00 0.00952 16000.00 10000.00 0.00 0.01046 16200.00 10000.00 0.00 0.01127 16400.00 10000.00 0.00 0.01210 16600.00 10000.00 0.00 0.01324 16800.00 10000.00 0.00 0.01451 17000.00 10000.00 0.00 0.01571 17200.00 10000.00 0.00 0.01698 17400.00 10000.00 0.00 0.01826 17600.00 10000.00 0.00 0.01946 17800.00 10000.00 0.00 0.02061 18000.00 10000.00 0.00 0.02167 18200.00 10000.00 0.00 0.02267 18400.00 10000.00 0.00 0.02371 18600.00 10000.00 0.00 0.02483 18800.00 10000.00 0.00 0.02593 19000.00 10000.00 0.00 0.02708 19200.00 10000.00 0.00 0.02831 19400.00 10000.00 0.00 0.02942 19600.00 10000.00 0.00 0.03059 19800.00 10000.00 0.00 0.03189 20000.00 10000.00 0.00 0.03339 20200.00 10000.00 0.00 0.03517 20400.00 10000.00 0.00 0.03697 20600.00 10000.00 0.00 0.03881 20800.00 10000.00 0.00 0.04044 21000.00 10000.00 0.00 0.04205 21200.00 10000.00 0.00 0.04372 21400.00 10000.00 0.00 0.04497 21600.00 10000.00 0.00 0.04564 21800.00 10000.00 0.00 0.04595 22000.00 10000.00 0.00 0.04637 22200.00 10000.00 0.00 0.04674 22400.00 10000.00 0.00 0.04684 22200.00 10200.00 0.00 0.04709 22400.00 10200.00 0.00 0.04714 22000.00 10400.00 0.00 0.04720 22200.00 10400.00 0.00 0.04749 22000.00 10600.00 0.00 0.04779 22200.00 10600.00 0.00 0.04805 21800.00 10800.00 0.00 0.04812 22000.00 10800.00 0.00 0.04856 22200.00 10800.00 0.00 0.04878 21800.00 11000.00 0.00 0.04884 22000.00 11000.00 0.00 0.04922 22200.00 11000.00 0.00 0.04939 21800.00 11200.00 0.00 0.04950 22000.00 11200.00 0.00 0.04985 22200.00 11200.00 0.00 0.04999 21800.00 11400.00 0.00 0.05016 22000.00 11400.00 0.00 0.05050 22200.00 11400.00 0.00 0.05064 22000.00 11600.00 0.00 0.05090 22200.00 11600.00 0.00 0.05105 21800.00 11800.00 0.00 0.05117 22000.00 11800.00 0.00 0.05157 22200.00 11800.00 0.00 0.05170 21800.00 12000.00 0.00 0.05193 22000.00 12000.00 0.00 0.05231 22200.00 12000.00 0.00 0.05238 21600.00 12200.00 0.00 0.05239 21800.00 12200.00 0.00 0.05269 22000.00 12200.00 0.00 0.05305 22200.00 12200.00 0.00 0.05313 21600.00 12400.00 0.00 0.05331 21800.00 12400.00 0.00 0.05361 22000.00 12400.00 0.00 0.05399 22200.00 12400.00 0.00 0.05411 21600.00 12600.00 0.00 0.05416 21800.00 12600.00 0.00 0.05447 22000.00 12600.00 0.00 0.05489 22200.00 12600.00 0.00 0.05506 21600.00 12800.00 0.00 0.05515 21800.00 12800.00 0.00 0.05552 22000.00 12800.00 0.00 0.05599 22200.00 12800.00 0.00 0.05619 21800.00 13000.00 0.00 0.05642 22000.00 13000.00 0.00 0.05692 22200.00 13000.00 0.00 0.05709 21800.00 13200.00 0.00 0.05737 22000.00 13200.00 0.00 0.05786 22200.00 13200.00 0.00 0.05800 21600.00 13400.00 0.00 0.05824 21800.00 13400.00 0.00 0.05876 22000.00 13400.00 0.00 0.05925 22200.00 13400.00 0.00 0.05937 21600.00 13600.00 0.00 0.05966 21800.00 13600.00 0.00 0.06016 22000.00 13600.00 0.00 0.06059 22200.00 13600.00 0.00 0.06068 21600.00 13800.00 0.00 0.06097 21800.00 13800.00 0.00 0.06144 22000.00 13800.00 0.00 0.06182 22200.00 13800.00 0.00 0.06190 21600.00 14000.00 0.00 0.06230 21800.00 14000.00 0.00 0.06274 22000.00 14000.00 0.00 0.06310 22200.00 14000.00 0.00 0.06318 21600.00 14200.00 0.00 0.06361 21800.00 14200.00 0.00 0.06402 22000.00 14200.00 0.00 0.06436 22200.00 14200.00 0.00 0.06445 21600.00 14400.00 0.00 0.06498 21800.00 14400.00 0.00 0.06537 22000.00 14400.00 0.00 0.06573 22200.00 14400.00 0.00 0.06582 21400.00 14600.00 0.00 0.06597 21600.00 14600.00 0.00 0.06655 21800.00 14600.00 0.00 0.06690 22000.00 14600.00 0.00 0.06724 22200.00 14600.00 0.00 0.06727 21400.00 14800.00 0.00 0.06752 21600.00 14800.00 0.00 0.06799 21800.00 14800.00 0.00 0.06830 22000.00 14800.00 0.00 0.06860 21400.00 15000.00 0.00 0.06880 21600.00 15000.00 0.00 0.06923 21800.00 15000.00 0.00 0.06955 22000.00 15000.00 0.00 0.06984 21400.00 15200.00 0.00 0.06988 21600.00 15200.00 0.00 0.07035 21800.00 15200.00 0.00 0.07071 22000.00 15200.00 0.00 0.07100 21600.00 15400.00 0.00 0.07149 21800.00 15400.00 0.00 0.07188 22000.00 15400.00 0.00 0.07215 21600.00 15600.00 0.00 0.07266 21800.00 15600.00 0.00 0.07305 22000.00 15600.00 0.00 0.07330 21400.00 15800.00 0.00 0.07331 21600.00 15800.00 0.00 0.07387 21800.00 15800.00 0.00 0.07424 22000.00 15800.00 0.00 0.07445 21400.00 16000.00 0.00 0.07460 21600.00 16000.00 0.00 0.07514 21800.00 16000.00 0.00 0.07549 22000.00 16000.00 0.00 0.07568 21400.00 16200.00 0.00 0.07593 21600.00 16200.00 0.00 0.07647 21800.00 16200.00 0.00 0.07683 22000.00 16200.00 0.00 0.07698 21400.00 16400.00 0.00 0.07744 21600.00 16400.00 0.00 0.07798 21800.00 16400.00 0.00 0.07831 22000.00 16400.00 0.00 0.07839 21400.00 16600.00 0.00 0.07876 21600.00 16600.00 0.00 0.07932 21800.00 16600.00 0.00 0.07962 22000.00 16600.00 0.00 0.07967 21400.00 16800.00 0.00 0.08037 21600.00 16800.00 0.00 0.08094 21800.00 16800.00 0.00 0.08120 21400.00 17000.00 0.00 0.08203 21600.00 17000.00 0.00 0.08252 21800.00 17000.00 0.00 0.08269 21400.00 17200.00 0.00 0.08346 21600.00 17200.00 0.00 0.08389 21800.00 17200.00 0.00 0.08402 21400.00 17400.00 0.00 0.08507 21600.00 17400.00 0.00 0.08547 21800.00 17400.00 0.00 0.08561 21400.00 17600.00 0.00 0.08641 21600.00 17600.00 0.00 0.08684 21800.00 17600.00 0.00 0.08700 21400.00 17800.00 0.00 0.08804 21600.00 17800.00 0.00 0.08850 21800.00 17800.00 0.00 0.08864 21400.00 18000.00 0.00 0.08964 21600.00 18000.00 0.00 0.09007 21800.00 18000.00 0.00 0.09012 21400.00 18200.00 0.00 0.09109 21600.00 18200.00 0.00 0.09152 21800.00 18200.00 0.00 0.09152 21200.00 18400.00 0.00 0.09172 21400.00 18400.00 0.00 0.09283 21600.00 18400.00 0.00 0.09324 21200.00 18600.00 0.00 0.09339 21400.00 18600.00 0.00 0.09445 21600.00 18600.00 0.00 0.09474 21200.00 18800.00 0.00 0.09499 21400.00 18800.00 0.00 0.09599 21600.00 18800.00 0.00 0.09623 21200.00 19000.00 0.00 0.09657 21400.00 19000.00 0.00 0.09757 21600.00 19000.00 0.00 0.09782 21200.00 19200.00 0.00 0.09843 21400.00 19200.00 0.00 0.09942 21600.00 19200.00 0.00 0.09962 21200.00 19400.00 0.00 0.10029 21400.00 19400.00 0.00 0.10128 21600.00 19400.00 0.00 0.10146 21200.00 19600.00 0.00 0.10241 21400.00 19600.00 0.00 0.10342 21600.00 19600.00 0.00 0.10358 21200.00 19800.00 0.00 0.10470 21400.00 19800.00 0.00 0.10566 21600.00 19800.00 0.00 0.10574 21200.00 20000.00 0.00 0.10683 21400.00 20000.00 0.00 0.10770 21200.00 20200.00 0.00 0.10887 21400.00 20200.00 0.00 0.10962 21200.00 20400.00 0.00 0.11084 21400.00 20400.00 0.00 0.11148 21200.00 20600.00 0.00 0.11323 21400.00 20600.00 0.00 0.11368 21200.00 20800.00 0.00 0.11545 21400.00 20800.00 0.00 0.11567 21000.00 21000.00 0.00 0.11616 21200.00 21000.00 0.00 0.11768 21000.00 21200.00 0.00 0.11805 21200.00 21200.00 0.00 0.11892 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21113.34 21350.98 41.28 0.11921 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/248.ctf NX, NY, NZ: 512 512 1 MODE : real