CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/247.mrc Output diagnostic file name Micrographs_0601/247.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/247.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2334.000 10376.00 Mean, RMS : 4497.881 219.0618 TITLE 1: emi2mrc: 247.emi Sun Jan 05 17:26:47 2014 TITLE 2: kV 300, Mag 78000x, Def 24120, Spot 4, Int 55.08%, Obj 88.08%, Dif 73.67% TITLE 3: X 61.161, Y 528.768, Z -81.867, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01207 10200.00 10000.00 0.00 -0.01180 10400.00 10000.00 0.00 -0.01153 10600.00 10000.00 0.00 -0.01151 11200.00 10000.00 0.00 -0.01140 11400.00 10000.00 0.00 -0.01132 11600.00 10000.00 0.00 -0.01125 11800.00 10000.00 0.00 -0.01120 12200.00 10000.00 0.00 -0.01110 12400.00 10000.00 0.00 -0.01086 12600.00 10000.00 0.00 -0.01055 12800.00 10000.00 0.00 -0.01025 13000.00 10000.00 0.00 -0.01001 13200.00 10000.00 0.00 -0.00973 13400.00 10000.00 0.00 -0.00948 13600.00 10000.00 0.00 -0.00917 13800.00 10000.00 0.00 -0.00884 14000.00 10000.00 0.00 -0.00837 14200.00 10000.00 0.00 -0.00786 14400.00 10000.00 0.00 -0.00743 14600.00 10000.00 0.00 -0.00689 14800.00 10000.00 0.00 -0.00638 15000.00 10000.00 0.00 -0.00595 15200.00 10000.00 0.00 -0.00537 15400.00 10000.00 0.00 -0.00463 15600.00 10000.00 0.00 -0.00381 15800.00 10000.00 0.00 -0.00309 16000.00 10000.00 0.00 -0.00237 16200.00 10000.00 0.00 -0.00150 16400.00 10000.00 0.00 -0.00066 16600.00 10000.00 0.00 0.00015 16800.00 10000.00 0.00 0.00107 17000.00 10000.00 0.00 0.00228 17200.00 10000.00 0.00 0.00355 17400.00 10000.00 0.00 0.00467 17600.00 10000.00 0.00 0.00580 17800.00 10000.00 0.00 0.00693 18000.00 10000.00 0.00 0.00811 18200.00 10000.00 0.00 0.00930 18400.00 10000.00 0.00 0.01043 18600.00 10000.00 0.00 0.01142 18800.00 10000.00 0.00 0.01222 19000.00 10000.00 0.00 0.01309 19200.00 10000.00 0.00 0.01408 19400.00 10000.00 0.00 0.01505 19600.00 10000.00 0.00 0.01610 19800.00 10000.00 0.00 0.01716 20000.00 10000.00 0.00 0.01818 20200.00 10000.00 0.00 0.01928 20400.00 10000.00 0.00 0.02057 20600.00 10000.00 0.00 0.02206 20800.00 10000.00 0.00 0.02354 21000.00 10000.00 0.00 0.02513 21200.00 10000.00 0.00 0.02692 21400.00 10000.00 0.00 0.02872 21600.00 10000.00 0.00 0.03050 21800.00 10000.00 0.00 0.03240 22000.00 10000.00 0.00 0.03452 22200.00 10000.00 0.00 0.03656 22400.00 10000.00 0.00 0.03833 22600.00 10000.00 0.00 0.03974 22800.00 10000.00 0.00 0.04059 23000.00 10000.00 0.00 0.04100 23200.00 10000.00 0.00 0.04113 23400.00 10000.00 0.00 0.04115 23000.00 10200.00 0.00 0.04123 23200.00 10200.00 0.00 0.04137 23400.00 10200.00 0.00 0.04141 23000.00 10400.00 0.00 0.04155 23200.00 10400.00 0.00 0.04175 23400.00 10400.00 0.00 0.04184 23000.00 10600.00 0.00 0.04196 23200.00 10600.00 0.00 0.04220 23400.00 10600.00 0.00 0.04228 23000.00 10800.00 0.00 0.04241 23200.00 10800.00 0.00 0.04264 23400.00 10800.00 0.00 0.04267 23000.00 11000.00 0.00 0.04291 23200.00 11000.00 0.00 0.04312 23000.00 11200.00 0.00 0.04330 23200.00 11200.00 0.00 0.04350 23000.00 11400.00 0.00 0.04365 23200.00 11400.00 0.00 0.04381 23000.00 11600.00 0.00 0.04403 23200.00 11600.00 0.00 0.04419 23000.00 11800.00 0.00 0.04464 23200.00 11800.00 0.00 0.04482 22800.00 12000.00 0.00 0.04493 23000.00 12000.00 0.00 0.04543 23200.00 12000.00 0.00 0.04557 22800.00 12200.00 0.00 0.04583 23000.00 12200.00 0.00 0.04627 23200.00 12200.00 0.00 0.04633 22800.00 12400.00 0.00 0.04668 23000.00 12400.00 0.00 0.04703 23200.00 12400.00 0.00 0.04704 22800.00 12600.00 0.00 0.04735 23000.00 12600.00 0.00 0.04765 23200.00 12600.00 0.00 0.04766 22800.00 12800.00 0.00 0.04813 23000.00 12800.00 0.00 0.04844 23200.00 12800.00 0.00 0.04848 22800.00 13000.00 0.00 0.04892 23000.00 13000.00 0.00 0.04928 23200.00 13000.00 0.00 0.04935 22800.00 13200.00 0.00 0.04972 23000.00 13200.00 0.00 0.05011 23200.00 13200.00 0.00 0.05016 22800.00 13400.00 0.00 0.05048 23000.00 13400.00 0.00 0.05085 23200.00 13400.00 0.00 0.05085 22800.00 13600.00 0.00 0.05121 23000.00 13600.00 0.00 0.05157 23200.00 13600.00 0.00 0.05159 22800.00 13800.00 0.00 0.05209 23000.00 13800.00 0.00 0.05249 23200.00 13800.00 0.00 0.05254 22800.00 14000.00 0.00 0.05302 23000.00 14000.00 0.00 0.05343 23200.00 14000.00 0.00 0.05344 22800.00 14200.00 0.00 0.05395 23000.00 14200.00 0.00 0.05432 22800.00 14400.00 0.00 0.05478 23000.00 14400.00 0.00 0.05515 22800.00 14600.00 0.00 0.05564 23000.00 14600.00 0.00 0.05606 22800.00 14800.00 0.00 0.05669 23000.00 14800.00 0.00 0.05711 22800.00 15000.00 0.00 0.05772 23000.00 15000.00 0.00 0.05809 22800.00 15200.00 0.00 0.05871 23000.00 15200.00 0.00 0.05907 22800.00 15400.00 0.00 0.05998 23000.00 15400.00 0.00 0.06032 22800.00 15600.00 0.00 0.06128 23000.00 15600.00 0.00 0.06158 22800.00 15800.00 0.00 0.06252 23000.00 15800.00 0.00 0.06278 22600.00 16000.00 0.00 0.06301 22800.00 16000.00 0.00 0.06392 23000.00 16000.00 0.00 0.06410 22600.00 16200.00 0.00 0.06437 22800.00 16200.00 0.00 0.06518 23000.00 16200.00 0.00 0.06529 22600.00 16400.00 0.00 0.06569 22800.00 16400.00 0.00 0.06643 23000.00 16400.00 0.00 0.06651 22600.00 16600.00 0.00 0.06695 22800.00 16600.00 0.00 0.06765 23000.00 16600.00 0.00 0.06773 22600.00 16800.00 0.00 0.06810 22800.00 16800.00 0.00 0.06879 23000.00 16800.00 0.00 0.06885 22600.00 17000.00 0.00 0.06921 22800.00 17000.00 0.00 0.06990 23000.00 17000.00 0.00 0.06999 22600.00 17200.00 0.00 0.07042 22800.00 17200.00 0.00 0.07113 23000.00 17200.00 0.00 0.07123 22600.00 17400.00 0.00 0.07187 22800.00 17400.00 0.00 0.07260 23000.00 17400.00 0.00 0.07273 22600.00 17600.00 0.00 0.07331 22800.00 17600.00 0.00 0.07407 23000.00 17600.00 0.00 0.07419 22600.00 17800.00 0.00 0.07477 22800.00 17800.00 0.00 0.07552 23000.00 17800.00 0.00 0.07563 22600.00 18000.00 0.00 0.07627 22800.00 18000.00 0.00 0.07701 23000.00 18000.00 0.00 0.07708 22600.00 18200.00 0.00 0.07783 22800.00 18200.00 0.00 0.07855 23000.00 18200.00 0.00 0.07858 22600.00 18400.00 0.00 0.07935 22800.00 18400.00 0.00 0.08008 23000.00 18400.00 0.00 0.08010 22600.00 18600.00 0.00 0.08116 22800.00 18600.00 0.00 0.08190 22600.00 18800.00 0.00 0.08321 22800.00 18800.00 0.00 0.08390 22600.00 19000.00 0.00 0.08502 22800.00 19000.00 0.00 0.08565 22600.00 19200.00 0.00 0.08686 22800.00 19200.00 0.00 0.08745 22600.00 19400.00 0.00 0.08882 22800.00 19400.00 0.00 0.08936 22600.00 19600.00 0.00 0.09099 22800.00 19600.00 0.00 0.09148 22600.00 19800.00 0.00 0.09305 22800.00 19800.00 0.00 0.09350 22400.00 20000.00 0.00 0.09363 22600.00 20000.00 0.00 0.09521 22800.00 20000.00 0.00 0.09565 22400.00 20200.00 0.00 0.09578 22600.00 20200.00 0.00 0.09737 22800.00 20200.00 0.00 0.09780 22600.00 20400.00 0.00 0.09940 22800.00 20400.00 0.00 0.09981 22400.00 20600.00 0.00 0.10012 22600.00 20600.00 0.00 0.10178 22800.00 20600.00 0.00 0.10213 22400.00 20800.00 0.00 0.10247 22600.00 20800.00 0.00 0.10412 22800.00 20800.00 0.00 0.10435 22400.00 21000.00 0.00 0.10514 22600.00 21000.00 0.00 0.10672 22800.00 21000.00 0.00 0.10677 22400.00 21200.00 0.00 0.10773 22600.00 21200.00 0.00 0.10919 22400.00 21400.00 0.00 0.11023 22600.00 21400.00 0.00 0.11159 22400.00 21600.00 0.00 0.11298 22600.00 21600.00 0.00 0.11426 22400.00 21800.00 0.00 0.11581 22600.00 21800.00 0.00 0.11694 22400.00 22000.00 0.00 0.11872 22600.00 22000.00 0.00 0.11956 22400.00 22200.00 0.00 0.12115 22600.00 22200.00 0.00 0.12161 22400.00 22400.00 0.00 0.12319 22400.00 22600.00 0.00 0.12329 22400.00 22600.00 5.00 0.12344 22400.00 22600.00 10.00 0.12359 22400.00 22600.00 15.00 0.12373 22400.00 22600.00 20.00 0.12385 22400.00 22600.00 25.00 0.12395 22400.00 22600.00 30.00 0.12402 22400.00 22600.00 35.00 0.12407 22400.00 22600.00 40.00 0.12409 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22387.12 22586.95 40.00 0.12411 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/247.ctf NX, NY, NZ: 512 512 1 MODE : real