CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/243.mrc Output diagnostic file name Micrographs_0601/243.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/243.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2264.000 8544.000 Mean, RMS : 4384.383 210.2006 TITLE 1: emi2mrc: 243.emi Sun Jan 05 19:55:59 2014 TITLE 2: kV 300, Mag 78000x, Def 27730, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 50.034, Y 519.696, Z -81.545, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01600 10200.00 10000.00 0.00 0.01693 10400.00 10000.00 0.00 0.01804 10600.00 10000.00 0.00 0.01928 10800.00 10000.00 0.00 0.02037 11000.00 10000.00 0.00 0.02162 11200.00 10000.00 0.00 0.02307 11400.00 10000.00 0.00 0.02444 11600.00 10000.00 0.00 0.02563 11800.00 10000.00 0.00 0.02681 12000.00 10000.00 0.00 0.02807 12200.00 10000.00 0.00 0.02923 12400.00 10000.00 0.00 0.03071 12600.00 10000.00 0.00 0.03238 12800.00 10000.00 0.00 0.03409 13000.00 10000.00 0.00 0.03609 13200.00 10000.00 0.00 0.03817 13400.00 10000.00 0.00 0.04006 13600.00 10000.00 0.00 0.04178 13800.00 10000.00 0.00 0.04361 14000.00 10000.00 0.00 0.04552 14200.00 10000.00 0.00 0.04756 14400.00 10000.00 0.00 0.04979 14600.00 10000.00 0.00 0.05217 14800.00 10000.00 0.00 0.05446 15000.00 10000.00 0.00 0.05670 15200.00 10000.00 0.00 0.05918 15400.00 10000.00 0.00 0.06164 15600.00 10000.00 0.00 0.06413 15800.00 10000.00 0.00 0.06636 16000.00 10000.00 0.00 0.06864 16200.00 10000.00 0.00 0.07093 16400.00 10000.00 0.00 0.07233 16600.00 10000.00 0.00 0.07323 16800.00 10000.00 0.00 0.07375 16600.00 10200.00 0.00 0.07451 16800.00 10200.00 0.00 0.07502 16600.00 10400.00 0.00 0.07594 16800.00 10400.00 0.00 0.07645 16400.00 10600.00 0.00 0.07655 16600.00 10600.00 0.00 0.07753 16800.00 10600.00 0.00 0.07802 16400.00 10800.00 0.00 0.07826 16600.00 10800.00 0.00 0.07922 16800.00 10800.00 0.00 0.07966 16400.00 11000.00 0.00 0.07999 16600.00 11000.00 0.00 0.08093 16800.00 11000.00 0.00 0.08129 16400.00 11200.00 0.00 0.08155 16600.00 11200.00 0.00 0.08250 16800.00 11200.00 0.00 0.08281 16400.00 11400.00 0.00 0.08340 16600.00 11400.00 0.00 0.08434 16800.00 11400.00 0.00 0.08457 16400.00 11600.00 0.00 0.08519 16600.00 11600.00 0.00 0.08607 16800.00 11600.00 0.00 0.08621 16400.00 11800.00 0.00 0.08689 16600.00 11800.00 0.00 0.08769 16800.00 11800.00 0.00 0.08778 16400.00 12000.00 0.00 0.08854 16600.00 12000.00 0.00 0.08925 16800.00 12000.00 0.00 0.08930 16400.00 12200.00 0.00 0.09036 16600.00 12200.00 0.00 0.09101 16800.00 12200.00 0.00 0.09105 16200.00 12400.00 0.00 0.09112 16400.00 12400.00 0.00 0.09242 16600.00 12400.00 0.00 0.09303 16400.00 12600.00 0.00 0.09425 16600.00 12600.00 0.00 0.09482 16200.00 12800.00 0.00 0.09512 16400.00 12800.00 0.00 0.09636 16600.00 12800.00 0.00 0.09689 16200.00 13000.00 0.00 0.09729 16400.00 13000.00 0.00 0.09845 16600.00 13000.00 0.00 0.09894 16200.00 13200.00 0.00 0.09932 16400.00 13200.00 0.00 0.10045 16600.00 13200.00 0.00 0.10091 16200.00 13400.00 0.00 0.10137 16400.00 13400.00 0.00 0.10253 16600.00 13400.00 0.00 0.10298 16200.00 13600.00 0.00 0.10370 16400.00 13600.00 0.00 0.10485 16600.00 13600.00 0.00 0.10523 16200.00 13800.00 0.00 0.10621 16400.00 13800.00 0.00 0.10729 16600.00 13800.00 0.00 0.10763 16200.00 14000.00 0.00 0.10875 16400.00 14000.00 0.00 0.10982 16600.00 14000.00 0.00 0.11013 16200.00 14200.00 0.00 0.11157 16400.00 14200.00 0.00 0.11259 16600.00 14200.00 0.00 0.11282 16200.00 14400.00 0.00 0.11419 16400.00 14400.00 0.00 0.11515 16600.00 14400.00 0.00 0.11525 16200.00 14600.00 0.00 0.11709 16400.00 14600.00 0.00 0.11794 16200.00 14800.00 0.00 0.12002 16400.00 14800.00 0.00 0.12071 16200.00 15000.00 0.00 0.12300 16400.00 15000.00 0.00 0.12356 16000.00 15200.00 0.00 0.12378 16200.00 15200.00 0.00 0.12605 16400.00 15200.00 0.00 0.12653 16000.00 15400.00 0.00 0.12678 16200.00 15400.00 0.00 0.12900 16400.00 15400.00 0.00 0.12935 16000.00 15600.00 0.00 0.12985 16200.00 15600.00 0.00 0.13198 16400.00 15600.00 0.00 0.13208 16000.00 15800.00 0.00 0.13243 16200.00 15800.00 0.00 0.13448 16000.00 16000.00 0.00 0.13533 16200.00 16000.00 0.00 0.13701 16000.00 16200.00 0.00 0.13713 16200.00 16200.00 0.00 0.13794 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 16129.66 16209.71 31.99 0.13815 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/243.ctf NX, NY, NZ: 512 512 1 MODE : real