CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/241.mrc Output diagnostic file name Micrographs_0601/241.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/241.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2142.000 8276.000 Mean, RMS : 4497.353 220.6618 TITLE 1: emi2mrc: 241.emi Sun Jan 05 17:56:11 2014 TITLE 2: kV 300, Mag 78000x, Def 25760, Spot 4, Int 55.08%, Obj 88.08%, Dif 73.67% TITLE 3: X 39.222, Y 524.024, Z -82.160, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00575 10200.00 10000.00 0.00 -0.00565 10400.00 10000.00 0.00 -0.00558 10600.00 10000.00 0.00 -0.00550 10800.00 10000.00 0.00 -0.00540 11000.00 10000.00 0.00 -0.00530 11200.00 10000.00 0.00 -0.00516 11400.00 10000.00 0.00 -0.00504 11600.00 10000.00 0.00 -0.00481 11800.00 10000.00 0.00 -0.00459 12000.00 10000.00 0.00 -0.00442 12200.00 10000.00 0.00 -0.00429 12400.00 10000.00 0.00 -0.00418 12600.00 10000.00 0.00 -0.00380 12800.00 10000.00 0.00 -0.00324 13000.00 10000.00 0.00 -0.00283 13200.00 10000.00 0.00 -0.00265 13400.00 10000.00 0.00 -0.00235 13600.00 10000.00 0.00 -0.00190 13800.00 10000.00 0.00 -0.00172 14000.00 10000.00 0.00 -0.00146 14200.00 10000.00 0.00 -0.00106 14400.00 10000.00 0.00 -0.00075 14600.00 10000.00 0.00 -0.00032 14800.00 10000.00 0.00 0.00021 15000.00 10000.00 0.00 0.00078 15200.00 10000.00 0.00 0.00143 15400.00 10000.00 0.00 0.00213 15600.00 10000.00 0.00 0.00281 15800.00 10000.00 0.00 0.00346 16000.00 10000.00 0.00 0.00413 16200.00 10000.00 0.00 0.00472 16400.00 10000.00 0.00 0.00543 16600.00 10000.00 0.00 0.00636 16800.00 10000.00 0.00 0.00724 17000.00 10000.00 0.00 0.00818 17200.00 10000.00 0.00 0.00926 17400.00 10000.00 0.00 0.01026 17600.00 10000.00 0.00 0.01129 17800.00 10000.00 0.00 0.01231 18000.00 10000.00 0.00 0.01330 18200.00 10000.00 0.00 0.01448 18400.00 10000.00 0.00 0.01573 18600.00 10000.00 0.00 0.01686 18800.00 10000.00 0.00 0.01809 19000.00 10000.00 0.00 0.01927 19200.00 10000.00 0.00 0.02037 19400.00 10000.00 0.00 0.02161 19600.00 10000.00 0.00 0.02286 19800.00 10000.00 0.00 0.02413 20000.00 10000.00 0.00 0.02541 20200.00 10000.00 0.00 0.02670 20400.00 10000.00 0.00 0.02797 20600.00 10000.00 0.00 0.02934 20800.00 10000.00 0.00 0.03088 21000.00 10000.00 0.00 0.03247 21200.00 10000.00 0.00 0.03421 21400.00 10000.00 0.00 0.03602 21600.00 10000.00 0.00 0.03795 21800.00 10000.00 0.00 0.04014 22000.00 10000.00 0.00 0.04249 22200.00 10000.00 0.00 0.04482 22400.00 10000.00 0.00 0.04678 22600.00 10000.00 0.00 0.04799 22800.00 10000.00 0.00 0.04848 23000.00 10000.00 0.00 0.04858 22600.00 10200.00 0.00 0.04858 22800.00 10200.00 0.00 0.04900 23000.00 10200.00 0.00 0.04906 22600.00 10400.00 0.00 0.04913 22800.00 10400.00 0.00 0.04952 23000.00 10400.00 0.00 0.04962 22600.00 10600.00 0.00 0.04973 22800.00 10600.00 0.00 0.05015 23000.00 10600.00 0.00 0.05029 22600.00 10800.00 0.00 0.05033 22800.00 10800.00 0.00 0.05076 23000.00 10800.00 0.00 0.05087 22800.00 11000.00 0.00 0.05131 23000.00 11000.00 0.00 0.05140 22600.00 11200.00 0.00 0.05143 22800.00 11200.00 0.00 0.05188 23000.00 11200.00 0.00 0.05196 22600.00 11400.00 0.00 0.05206 22800.00 11400.00 0.00 0.05252 23000.00 11400.00 0.00 0.05260 22600.00 11600.00 0.00 0.05278 22800.00 11600.00 0.00 0.05323 23000.00 11600.00 0.00 0.05328 22600.00 11800.00 0.00 0.05366 22800.00 11800.00 0.00 0.05405 22600.00 12000.00 0.00 0.05439 22800.00 12000.00 0.00 0.05469 22600.00 12200.00 0.00 0.05502 22800.00 12200.00 0.00 0.05528 22600.00 12400.00 0.00 0.05569 22800.00 12400.00 0.00 0.05595 22600.00 12600.00 0.00 0.05625 22800.00 12600.00 0.00 0.05651 22600.00 12800.00 0.00 0.05692 22800.00 12800.00 0.00 0.05715 22600.00 13000.00 0.00 0.05765 22800.00 13000.00 0.00 0.05790 23000.00 13000.00 0.00 0.05791 22600.00 13200.00 0.00 0.05847 22800.00 13200.00 0.00 0.05877 23000.00 13200.00 0.00 0.05882 22600.00 13400.00 0.00 0.05938 22800.00 13400.00 0.00 0.05971 23000.00 13400.00 0.00 0.05976 22600.00 13600.00 0.00 0.06034 22800.00 13600.00 0.00 0.06066 22600.00 13800.00 0.00 0.06125 22800.00 13800.00 0.00 0.06154 22600.00 14000.00 0.00 0.06217 22800.00 14000.00 0.00 0.06248 22600.00 14200.00 0.00 0.06326 22800.00 14200.00 0.00 0.06354 22600.00 14400.00 0.00 0.06427 22800.00 14400.00 0.00 0.06449 22600.00 14600.00 0.00 0.06527 22800.00 14600.00 0.00 0.06543 22600.00 14800.00 0.00 0.06616 22800.00 14800.00 0.00 0.06630 22600.00 15000.00 0.00 0.06714 22800.00 15000.00 0.00 0.06731 22600.00 15200.00 0.00 0.06839 22800.00 15200.00 0.00 0.06856 22400.00 15400.00 0.00 0.06858 22600.00 15400.00 0.00 0.06966 22800.00 15400.00 0.00 0.06976 22400.00 15600.00 0.00 0.06983 22600.00 15600.00 0.00 0.07088 22800.00 15600.00 0.00 0.07093 22400.00 15800.00 0.00 0.07119 22600.00 15800.00 0.00 0.07221 22800.00 15800.00 0.00 0.07223 22400.00 16000.00 0.00 0.07272 22600.00 16000.00 0.00 0.07371 22400.00 16200.00 0.00 0.07425 22600.00 16200.00 0.00 0.07517 22400.00 16400.00 0.00 0.07576 22600.00 16400.00 0.00 0.07661 22400.00 16600.00 0.00 0.07716 22600.00 16600.00 0.00 0.07797 22400.00 16800.00 0.00 0.07867 22600.00 16800.00 0.00 0.07948 22400.00 17000.00 0.00 0.08025 22600.00 17000.00 0.00 0.08104 22400.00 17200.00 0.00 0.08173 22600.00 17200.00 0.00 0.08252 22400.00 17400.00 0.00 0.08338 22600.00 17400.00 0.00 0.08420 22400.00 17600.00 0.00 0.08512 22600.00 17600.00 0.00 0.08596 22400.00 17800.00 0.00 0.08687 22600.00 17800.00 0.00 0.08771 22400.00 18000.00 0.00 0.08864 22600.00 18000.00 0.00 0.08952 22400.00 18200.00 0.00 0.09068 22600.00 18200.00 0.00 0.09154 22400.00 18400.00 0.00 0.09265 22600.00 18400.00 0.00 0.09342 22400.00 18600.00 0.00 0.09448 22600.00 18600.00 0.00 0.09519 22400.00 18800.00 0.00 0.09650 22600.00 18800.00 0.00 0.09717 22400.00 19000.00 0.00 0.09853 22600.00 19000.00 0.00 0.09915 22400.00 19200.00 0.00 0.10058 22600.00 19200.00 0.00 0.10111 22400.00 19400.00 0.00 0.10242 22600.00 19400.00 0.00 0.10290 22400.00 19600.00 0.00 0.10447 22600.00 19600.00 0.00 0.10491 22400.00 19800.00 0.00 0.10666 22600.00 19800.00 0.00 0.10708 22400.00 20000.00 0.00 0.10886 22600.00 20000.00 0.00 0.10920 22400.00 20200.00 0.00 0.11108 22600.00 20200.00 0.00 0.11132 22400.00 20400.00 0.00 0.11341 22600.00 20400.00 0.00 0.11351 22200.00 20600.00 0.00 0.11381 22400.00 20600.00 0.00 0.11608 22200.00 20800.00 0.00 0.11645 22400.00 20800.00 0.00 0.11861 22200.00 21000.00 0.00 0.11920 22400.00 21000.00 0.00 0.12125 22200.00 21200.00 0.00 0.12184 22400.00 21200.00 0.00 0.12381 22200.00 21400.00 0.00 0.12466 22400.00 21400.00 0.00 0.12649 22200.00 21600.00 0.00 0.12771 22400.00 21600.00 0.00 0.12931 22200.00 21800.00 0.00 0.13060 22400.00 21800.00 0.00 0.13187 22200.00 22000.00 0.00 0.13358 22400.00 22000.00 0.00 0.13438 22200.00 22200.00 0.00 0.13583 22400.00 22200.00 0.00 0.13607 22200.00 22400.00 0.00 0.13696 22200.00 22400.00 5.00 0.13713 22200.00 22400.00 10.00 0.13728 22200.00 22400.00 15.00 0.13741 22200.00 22400.00 20.00 0.13751 22200.00 22400.00 25.00 0.13758 22200.00 22400.00 30.00 0.13762 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22214.00 22420.63 30.00 0.13765 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/241.ctf NX, NY, NZ: 512 512 1 MODE : real