CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/230.mrc Output diagnostic file name Micrographs_0601/230.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/230.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2038.000 8972.000 Mean, RMS : 4676.751 222.4630 TITLE 1: emi2mrc: 230.emi Sun Jan 05 19:46:59 2014 TITLE 2: kV 300, Mag 78000x, Def 27890, Spot 4, Int 55.05%, Obj 88.05%, Dif 73.67% TITLE 3: X 34.075, Y 524.020, Z -81.545, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01148 16000.00 10000.00 0.00 -0.01135 16200.00 10000.00 0.00 -0.01088 16400.00 10000.00 0.00 -0.01035 16600.00 10000.00 0.00 -0.00983 16800.00 10000.00 0.00 -0.00951 17000.00 10000.00 0.00 -0.00948 17200.00 10000.00 0.00 -0.00925 17400.00 10000.00 0.00 -0.00881 17600.00 10000.00 0.00 -0.00832 17800.00 10000.00 0.00 -0.00773 18000.00 10000.00 0.00 -0.00715 18200.00 10000.00 0.00 -0.00661 18400.00 10000.00 0.00 -0.00607 18600.00 10000.00 0.00 -0.00560 18800.00 10000.00 0.00 -0.00523 19000.00 10000.00 0.00 -0.00475 19200.00 10000.00 0.00 -0.00410 19400.00 10000.00 0.00 -0.00347 19600.00 10000.00 0.00 -0.00279 19800.00 10000.00 0.00 -0.00203 20000.00 10000.00 0.00 -0.00126 20200.00 10000.00 0.00 -0.00067 20400.00 10000.00 0.00 -0.00020 20600.00 10000.00 0.00 0.00041 20800.00 10000.00 0.00 0.00093 21000.00 10000.00 0.00 0.00133 21200.00 10000.00 0.00 0.00191 21400.00 10000.00 0.00 0.00266 21600.00 10000.00 0.00 0.00349 21800.00 10000.00 0.00 0.00433 22000.00 10000.00 0.00 0.00508 22200.00 10000.00 0.00 0.00594 22400.00 10000.00 0.00 0.00713 22600.00 10000.00 0.00 0.00833 22800.00 10000.00 0.00 0.00936 23000.00 10000.00 0.00 0.01036 23200.00 10000.00 0.00 0.01128 23400.00 10000.00 0.00 0.01223 23600.00 10000.00 0.00 0.01332 23800.00 10000.00 0.00 0.01454 24000.00 10000.00 0.00 0.01583 24200.00 10000.00 0.00 0.01702 24400.00 10000.00 0.00 0.01830 24600.00 10000.00 0.00 0.01996 24800.00 10000.00 0.00 0.02177 25000.00 10000.00 0.00 0.02371 25200.00 10000.00 0.00 0.02579 25400.00 10000.00 0.00 0.02789 25600.00 10000.00 0.00 0.03011 25800.00 10000.00 0.00 0.03175 26000.00 10000.00 0.00 0.03246 26200.00 10000.00 0.00 0.03290 26400.00 10000.00 0.00 0.03321 26600.00 10000.00 0.00 0.03330 26200.00 10200.00 0.00 0.03347 26400.00 10200.00 0.00 0.03375 26600.00 10200.00 0.00 0.03378 26200.00 10400.00 0.00 0.03401 26400.00 10400.00 0.00 0.03422 26200.00 10600.00 0.00 0.03447 26400.00 10600.00 0.00 0.03464 26200.00 10800.00 0.00 0.03481 26400.00 10800.00 0.00 0.03497 26200.00 11000.00 0.00 0.03500 26400.00 11000.00 0.00 0.03510 26200.00 11200.00 0.00 0.03512 26400.00 11200.00 0.00 0.03517 26200.00 11400.00 0.00 0.03520 26400.00 11400.00 0.00 0.03524 26200.00 11600.00 0.00 0.03537 26400.00 11600.00 0.00 0.03543 26200.00 11800.00 0.00 0.03569 26400.00 11800.00 0.00 0.03577 26200.00 12000.00 0.00 0.03598 26400.00 12000.00 0.00 0.03606 26200.00 12200.00 0.00 0.03626 26400.00 12200.00 0.00 0.03632 26200.00 12400.00 0.00 0.03665 26400.00 12400.00 0.00 0.03667 26000.00 12600.00 0.00 0.03676 26200.00 12600.00 0.00 0.03704 26400.00 12600.00 0.00 0.03706 26600.00 12600.00 0.00 0.03707 26000.00 12800.00 0.00 0.03710 26200.00 12800.00 0.00 0.03737 26400.00 12800.00 0.00 0.03745 26600.00 12800.00 0.00 0.03750 26200.00 13000.00 0.00 0.03772 26400.00 13000.00 0.00 0.03786 26600.00 13000.00 0.00 0.03792 26200.00 13200.00 0.00 0.03802 26400.00 13200.00 0.00 0.03817 26600.00 13200.00 0.00 0.03818 26200.00 13400.00 0.00 0.03822 26400.00 13400.00 0.00 0.03838 26200.00 13600.00 0.00 0.03864 26400.00 13600.00 0.00 0.03882 26200.00 13800.00 0.00 0.03918 26400.00 13800.00 0.00 0.03934 26200.00 14000.00 0.00 0.03985 26400.00 14000.00 0.00 0.03998 26000.00 14200.00 0.00 0.04027 26200.00 14200.00 0.00 0.04077 26400.00 14200.00 0.00 0.04081 26000.00 14400.00 0.00 0.04122 26200.00 14400.00 0.00 0.04161 26000.00 14600.00 0.00 0.04208 26200.00 14600.00 0.00 0.04241 26400.00 14600.00 0.00 0.04243 26600.00 14600.00 0.00 0.04249 26000.00 14800.00 0.00 0.04278 26200.00 14800.00 0.00 0.04310 26400.00 14800.00 0.00 0.04317 26600.00 14800.00 0.00 0.04325 26000.00 15000.00 0.00 0.04342 26200.00 15000.00 0.00 0.04374 26400.00 15000.00 0.00 0.04383 26600.00 15000.00 0.00 0.04388 26000.00 15200.00 0.00 0.04399 26200.00 15200.00 0.00 0.04430 26400.00 15200.00 0.00 0.04439 26600.00 15200.00 0.00 0.04441 26000.00 15400.00 0.00 0.04451 26200.00 15400.00 0.00 0.04480 26400.00 15400.00 0.00 0.04491 26600.00 15400.00 0.00 0.04492 26000.00 15600.00 0.00 0.04508 26200.00 15600.00 0.00 0.04541 26400.00 15600.00 0.00 0.04556 26000.00 15800.00 0.00 0.04571 26200.00 15800.00 0.00 0.04612 26400.00 15800.00 0.00 0.04628 26000.00 16000.00 0.00 0.04644 26200.00 16000.00 0.00 0.04687 26400.00 16000.00 0.00 0.04696 26000.00 16200.00 0.00 0.04711 26200.00 16200.00 0.00 0.04750 26400.00 16200.00 0.00 0.04753 26000.00 16400.00 0.00 0.04778 26200.00 16400.00 0.00 0.04814 26400.00 16400.00 0.00 0.04816 26000.00 16600.00 0.00 0.04854 26200.00 16600.00 0.00 0.04889 26400.00 16600.00 0.00 0.04891 26000.00 16800.00 0.00 0.04944 26200.00 16800.00 0.00 0.04980 26400.00 16800.00 0.00 0.04982 26000.00 17000.00 0.00 0.05041 26200.00 17000.00 0.00 0.05076 26400.00 17000.00 0.00 0.05077 26000.00 17200.00 0.00 0.05133 26200.00 17200.00 0.00 0.05163 26000.00 17400.00 0.00 0.05217 26200.00 17400.00 0.00 0.05245 26000.00 17600.00 0.00 0.05300 26200.00 17600.00 0.00 0.05328 26000.00 17800.00 0.00 0.05398 26200.00 17800.00 0.00 0.05427 26000.00 18000.00 0.00 0.05497 26200.00 18000.00 0.00 0.05526 26000.00 18200.00 0.00 0.05611 26200.00 18200.00 0.00 0.05638 26000.00 18400.00 0.00 0.05733 26200.00 18400.00 0.00 0.05756 26000.00 18600.00 0.00 0.05842 26200.00 18600.00 0.00 0.05864 26000.00 18800.00 0.00 0.05958 26200.00 18800.00 0.00 0.05981 26000.00 19000.00 0.00 0.06078 26200.00 19000.00 0.00 0.06100 25800.00 19200.00 0.00 0.06107 26000.00 19200.00 0.00 0.06217 26200.00 19200.00 0.00 0.06230 25800.00 19400.00 0.00 0.06259 26000.00 19400.00 0.00 0.06362 26200.00 19400.00 0.00 0.06362 25800.00 19600.00 0.00 0.06408 26000.00 19600.00 0.00 0.06498 25800.00 19800.00 0.00 0.06538 26000.00 19800.00 0.00 0.06622 25800.00 20000.00 0.00 0.06672 26000.00 20000.00 0.00 0.06756 25800.00 20200.00 0.00 0.06816 26000.00 20200.00 0.00 0.06899 25800.00 20400.00 0.00 0.06958 26000.00 20400.00 0.00 0.07035 25800.00 20600.00 0.00 0.07106 26000.00 20600.00 0.00 0.07176 25800.00 20800.00 0.00 0.07231 26000.00 20800.00 0.00 0.07298 25800.00 21000.00 0.00 0.07350 26000.00 21000.00 0.00 0.07419 25800.00 21200.00 0.00 0.07483 26000.00 21200.00 0.00 0.07555 25800.00 21400.00 0.00 0.07627 26000.00 21400.00 0.00 0.07701 25800.00 21600.00 0.00 0.07775 26000.00 21600.00 0.00 0.07849 25800.00 21800.00 0.00 0.07930 26000.00 21800.00 0.00 0.08004 25800.00 22000.00 0.00 0.08104 26000.00 22000.00 0.00 0.08177 25800.00 22200.00 0.00 0.08276 26000.00 22200.00 0.00 0.08347 25800.00 22400.00 0.00 0.08462 26000.00 22400.00 0.00 0.08533 25800.00 22600.00 0.00 0.08650 26000.00 22600.00 0.00 0.08713 25800.00 22800.00 0.00 0.08837 26000.00 22800.00 0.00 0.08894 25800.00 23000.00 0.00 0.09032 26000.00 23000.00 0.00 0.09088 25800.00 23200.00 0.00 0.09252 26000.00 23200.00 0.00 0.09301 25800.00 23400.00 0.00 0.09482 26000.00 23400.00 0.00 0.09517 25800.00 23600.00 0.00 0.09696 26000.00 23600.00 0.00 0.09726 25800.00 23800.00 0.00 0.09955 26000.00 23800.00 0.00 0.09978 25600.00 24000.00 0.00 0.09993 25800.00 24000.00 0.00 0.10224 26000.00 24000.00 0.00 0.10232 25600.00 24200.00 0.00 0.10267 25800.00 24200.00 0.00 0.10494 25600.00 24400.00 0.00 0.10561 25800.00 24400.00 0.00 0.10781 25600.00 24600.00 0.00 0.10875 25800.00 24600.00 0.00 0.11083 25600.00 24800.00 0.00 0.11207 25800.00 24800.00 0.00 0.11406 25600.00 25000.00 0.00 0.11559 25800.00 25000.00 0.00 0.11744 25600.00 25200.00 0.00 0.11937 25800.00 25200.00 0.00 0.12087 25600.00 25400.00 0.00 0.12281 25800.00 25400.00 0.00 0.12382 25600.00 25600.00 0.00 0.12587 25800.00 25600.00 0.00 0.12611 25600.00 25800.00 0.00 0.12695 25600.00 25800.00 5.00 0.12720 25600.00 25800.00 10.00 0.12743 25600.00 25800.00 15.00 0.12764 25600.00 25800.00 20.00 0.12782 25600.00 25800.00 25.00 0.12796 25600.00 25800.00 30.00 0.12806 25600.00 25800.00 35.00 0.12811 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25590.34 25853.01 37.85 0.12820 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/230.ctf NX, NY, NZ: 512 512 1 MODE : real