CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/227.mrc Output diagnostic file name Micrographs_0601/227.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/227.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2630.000 8176.000 Mean, RMS : 4500.852 222.9131 TITLE 1: emi2mrc: 227.emi Sun Jan 05 17:54:53 2014 TITLE 2: kV 300, Mag 78000x, Def 25760, Spot 4, Int 55.08%, Obj 88.08%, Dif 73.67% TITLE 3: X 44.257, Y 523.304, Z -82.160, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00933 10200.00 10000.00 0.00 -0.00924 10400.00 10000.00 0.00 -0.00903 10600.00 10000.00 0.00 -0.00884 10800.00 10000.00 0.00 -0.00855 11000.00 10000.00 0.00 -0.00830 11200.00 10000.00 0.00 -0.00800 11400.00 10000.00 0.00 -0.00772 11600.00 10000.00 0.00 -0.00756 11800.00 10000.00 0.00 -0.00742 12000.00 10000.00 0.00 -0.00712 12200.00 10000.00 0.00 -0.00664 12400.00 10000.00 0.00 -0.00635 12600.00 10000.00 0.00 -0.00609 12800.00 10000.00 0.00 -0.00581 13000.00 10000.00 0.00 -0.00545 13200.00 10000.00 0.00 -0.00500 13400.00 10000.00 0.00 -0.00459 13600.00 10000.00 0.00 -0.00404 13800.00 10000.00 0.00 -0.00334 14000.00 10000.00 0.00 -0.00262 14200.00 10000.00 0.00 -0.00193 14400.00 10000.00 0.00 -0.00122 14600.00 10000.00 0.00 -0.00051 14800.00 10000.00 0.00 0.00003 15000.00 10000.00 0.00 0.00047 15200.00 10000.00 0.00 0.00101 15400.00 10000.00 0.00 0.00163 15600.00 10000.00 0.00 0.00238 15800.00 10000.00 0.00 0.00335 16000.00 10000.00 0.00 0.00432 16200.00 10000.00 0.00 0.00521 16400.00 10000.00 0.00 0.00616 16600.00 10000.00 0.00 0.00718 16800.00 10000.00 0.00 0.00833 17000.00 10000.00 0.00 0.00955 17200.00 10000.00 0.00 0.01068 17400.00 10000.00 0.00 0.01176 17600.00 10000.00 0.00 0.01278 17800.00 10000.00 0.00 0.01383 18000.00 10000.00 0.00 0.01490 18200.00 10000.00 0.00 0.01593 18400.00 10000.00 0.00 0.01708 18600.00 10000.00 0.00 0.01828 18800.00 10000.00 0.00 0.01942 19000.00 10000.00 0.00 0.02060 19200.00 10000.00 0.00 0.02190 19400.00 10000.00 0.00 0.02312 19600.00 10000.00 0.00 0.02438 19800.00 10000.00 0.00 0.02572 20000.00 10000.00 0.00 0.02704 20200.00 10000.00 0.00 0.02859 20400.00 10000.00 0.00 0.03019 20600.00 10000.00 0.00 0.03188 20800.00 10000.00 0.00 0.03371 21000.00 10000.00 0.00 0.03548 21200.00 10000.00 0.00 0.03737 21400.00 10000.00 0.00 0.03946 21600.00 10000.00 0.00 0.04178 21800.00 10000.00 0.00 0.04373 22000.00 10000.00 0.00 0.04480 22200.00 10000.00 0.00 0.04527 22400.00 10000.00 0.00 0.04533 22200.00 10200.00 0.00 0.04565 22200.00 10400.00 0.00 0.04607 22000.00 10600.00 0.00 0.04618 22200.00 10600.00 0.00 0.04655 22000.00 10800.00 0.00 0.04663 22200.00 10800.00 0.00 0.04694 22000.00 11000.00 0.00 0.04710 22200.00 11000.00 0.00 0.04739 22000.00 11200.00 0.00 0.04757 22200.00 11200.00 0.00 0.04789 22000.00 11400.00 0.00 0.04818 22200.00 11400.00 0.00 0.04851 22000.00 11600.00 0.00 0.04887 22200.00 11600.00 0.00 0.04919 22000.00 11800.00 0.00 0.04956 22200.00 11800.00 0.00 0.04988 22400.00 11800.00 0.00 0.04988 22000.00 12000.00 0.00 0.05034 22200.00 12000.00 0.00 0.05068 22400.00 12000.00 0.00 0.05071 22000.00 12200.00 0.00 0.05131 22200.00 12200.00 0.00 0.05167 22400.00 12200.00 0.00 0.05170 22000.00 12400.00 0.00 0.05240 22200.00 12400.00 0.00 0.05276 22400.00 12400.00 0.00 0.05280 22000.00 12600.00 0.00 0.05350 22200.00 12600.00 0.00 0.05384 22400.00 12600.00 0.00 0.05385 22000.00 12800.00 0.00 0.05445 22200.00 12800.00 0.00 0.05476 22000.00 13000.00 0.00 0.05529 22200.00 13000.00 0.00 0.05562 22000.00 13200.00 0.00 0.05638 22200.00 13200.00 0.00 0.05672 22000.00 13400.00 0.00 0.05740 22200.00 13400.00 0.00 0.05770 22000.00 13600.00 0.00 0.05848 22200.00 13600.00 0.00 0.05873 22000.00 13800.00 0.00 0.05962 22200.00 13800.00 0.00 0.05984 22000.00 14000.00 0.00 0.06073 22200.00 14000.00 0.00 0.06097 22400.00 14000.00 0.00 0.06102 22600.00 14000.00 0.00 0.06102 22000.00 14200.00 0.00 0.06203 22200.00 14200.00 0.00 0.06231 22400.00 14200.00 0.00 0.06237 22000.00 14400.00 0.00 0.06322 22200.00 14400.00 0.00 0.06350 22400.00 14400.00 0.00 0.06351 22000.00 14600.00 0.00 0.06443 22200.00 14600.00 0.00 0.06469 22000.00 14800.00 0.00 0.06563 22200.00 14800.00 0.00 0.06590 22000.00 15000.00 0.00 0.06693 22200.00 15000.00 0.00 0.06724 22000.00 15200.00 0.00 0.06835 22200.00 15200.00 0.00 0.06865 22000.00 15400.00 0.00 0.06971 22200.00 15400.00 0.00 0.06996 21800.00 15600.00 0.00 0.07003 22000.00 15600.00 0.00 0.07110 22200.00 15600.00 0.00 0.07135 22000.00 15800.00 0.00 0.07239 22200.00 15800.00 0.00 0.07270 22000.00 16000.00 0.00 0.07384 22200.00 16000.00 0.00 0.07418 22000.00 16200.00 0.00 0.07538 22200.00 16200.00 0.00 0.07566 21800.00 16400.00 0.00 0.07578 22000.00 16400.00 0.00 0.07701 22200.00 16400.00 0.00 0.07719 21800.00 16600.00 0.00 0.07742 22000.00 16600.00 0.00 0.07863 22200.00 16600.00 0.00 0.07870 21800.00 16800.00 0.00 0.07901 22000.00 16800.00 0.00 0.08014 21800.00 17000.00 0.00 0.08054 22000.00 17000.00 0.00 0.08160 21800.00 17200.00 0.00 0.08196 22000.00 17200.00 0.00 0.08302 21800.00 17400.00 0.00 0.08358 22000.00 17400.00 0.00 0.08467 21800.00 17600.00 0.00 0.08523 22000.00 17600.00 0.00 0.08632 21800.00 17800.00 0.00 0.08712 22000.00 17800.00 0.00 0.08823 21800.00 18000.00 0.00 0.08914 22000.00 18000.00 0.00 0.09025 21800.00 18200.00 0.00 0.09110 22000.00 18200.00 0.00 0.09215 21800.00 18400.00 0.00 0.09303 22000.00 18400.00 0.00 0.09399 21800.00 18600.00 0.00 0.09496 22000.00 18600.00 0.00 0.09586 21800.00 18800.00 0.00 0.09694 22000.00 18800.00 0.00 0.09780 21800.00 19000.00 0.00 0.09893 22000.00 19000.00 0.00 0.09980 21800.00 19200.00 0.00 0.10126 22000.00 19200.00 0.00 0.10211 21800.00 19400.00 0.00 0.10348 22000.00 19400.00 0.00 0.10426 21800.00 19600.00 0.00 0.10590 22000.00 19600.00 0.00 0.10660 21800.00 19800.00 0.00 0.10851 22000.00 19800.00 0.00 0.10915 21800.00 20000.00 0.00 0.11118 22000.00 20000.00 0.00 0.11174 21800.00 20200.00 0.00 0.11429 22000.00 20200.00 0.00 0.11476 21800.00 20400.00 0.00 0.11743 22000.00 20400.00 0.00 0.11773 21600.00 20600.00 0.00 0.11791 21800.00 20600.00 0.00 0.12065 22000.00 20600.00 0.00 0.12071 21600.00 20800.00 0.00 0.12118 21800.00 20800.00 0.00 0.12380 21600.00 21000.00 0.00 0.12469 21800.00 21000.00 0.00 0.12709 21600.00 21200.00 0.00 0.12820 21800.00 21200.00 0.00 0.13030 21600.00 21400.00 0.00 0.13182 21800.00 21400.00 0.00 0.13343 21600.00 21600.00 0.00 0.13539 21800.00 21600.00 0.00 0.13607 21600.00 21800.00 0.00 0.13694 21600.00 21800.00 5.00 0.13713 21600.00 21800.00 10.00 0.13729 21600.00 21800.00 15.00 0.13744 21600.00 21800.00 20.00 0.13756 21600.00 21800.00 25.00 0.13764 21600.00 21800.00 30.00 0.13769 21600.00 21800.00 35.00 0.13769 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21629.55 21856.27 30.94 0.13796 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/227.ctf NX, NY, NZ: 512 512 1 MODE : real