CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/216.mrc Output diagnostic file name Micrographs_0601/216.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/216.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2468.000 7776.000 Mean, RMS : 4507.462 217.0369 TITLE 1: emi2mrc: 216.emi Sun Jan 05 17:46:06 2014 TITLE 2: kV 300, Mag 78000x, Def 25760, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 50.485, Y 524.921, Z -81.406, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02523 10200.00 10000.00 0.00 0.02641 10400.00 10000.00 0.00 0.02747 10600.00 10000.00 0.00 0.02872 10800.00 10000.00 0.00 0.03040 11000.00 10000.00 0.00 0.03218 11200.00 10000.00 0.00 0.03365 11400.00 10000.00 0.00 0.03513 11600.00 10000.00 0.00 0.03683 11800.00 10000.00 0.00 0.03875 12000.00 10000.00 0.00 0.04082 12200.00 10000.00 0.00 0.04269 12400.00 10000.00 0.00 0.04487 12600.00 10000.00 0.00 0.04722 12800.00 10000.00 0.00 0.04963 13000.00 10000.00 0.00 0.05226 13200.00 10000.00 0.00 0.05489 13400.00 10000.00 0.00 0.05755 13600.00 10000.00 0.00 0.06029 13800.00 10000.00 0.00 0.06331 14000.00 10000.00 0.00 0.06621 14200.00 10000.00 0.00 0.06897 14400.00 10000.00 0.00 0.07193 14600.00 10000.00 0.00 0.07513 14800.00 10000.00 0.00 0.07849 15000.00 10000.00 0.00 0.08192 15200.00 10000.00 0.00 0.08513 15400.00 10000.00 0.00 0.08703 15600.00 10000.00 0.00 0.08767 15800.00 10000.00 0.00 0.08768 15400.00 10200.00 0.00 0.08865 15600.00 10200.00 0.00 0.08927 15400.00 10400.00 0.00 0.09026 15600.00 10400.00 0.00 0.09092 15400.00 10600.00 0.00 0.09220 15600.00 10600.00 0.00 0.09292 15400.00 10800.00 0.00 0.09428 15600.00 10800.00 0.00 0.09499 15400.00 11000.00 0.00 0.09672 15600.00 11000.00 0.00 0.09737 15400.00 11200.00 0.00 0.09915 15600.00 11200.00 0.00 0.09975 15200.00 11400.00 0.00 0.09975 15400.00 11400.00 0.00 0.10181 15600.00 11400.00 0.00 0.10235 15200.00 11600.00 0.00 0.10250 15400.00 11600.00 0.00 0.10452 15600.00 11600.00 0.00 0.10498 15200.00 11800.00 0.00 0.10531 15400.00 11800.00 0.00 0.10726 15600.00 11800.00 0.00 0.10760 15200.00 12000.00 0.00 0.10808 15400.00 12000.00 0.00 0.10998 15600.00 12000.00 0.00 0.11024 15200.00 12200.00 0.00 0.11090 15400.00 12200.00 0.00 0.11281 15600.00 12200.00 0.00 0.11301 15200.00 12400.00 0.00 0.11414 15400.00 12400.00 0.00 0.11601 15600.00 12400.00 0.00 0.11606 15200.00 12600.00 0.00 0.11717 15400.00 12600.00 0.00 0.11893 15200.00 12800.00 0.00 0.12019 15400.00 12800.00 0.00 0.12187 15200.00 13000.00 0.00 0.12328 15400.00 13000.00 0.00 0.12492 15200.00 13200.00 0.00 0.12665 15400.00 13200.00 0.00 0.12826 15200.00 13400.00 0.00 0.12995 15400.00 13400.00 0.00 0.13150 15200.00 13600.00 0.00 0.13334 15400.00 13600.00 0.00 0.13478 15200.00 13800.00 0.00 0.13691 15400.00 13800.00 0.00 0.13811 15200.00 14000.00 0.00 0.14034 15400.00 14000.00 0.00 0.14129 15200.00 14200.00 0.00 0.14414 15400.00 14200.00 0.00 0.14481 15200.00 14400.00 0.00 0.14788 15400.00 14400.00 0.00 0.14824 15000.00 14600.00 0.00 0.14844 15200.00 14600.00 0.00 0.15198 15000.00 14800.00 0.00 0.15232 15200.00 14800.00 0.00 0.15571 15000.00 15000.00 0.00 0.15634 15200.00 15000.00 0.00 0.15930 15000.00 15200.00 0.00 0.15967 15200.00 15200.00 0.00 0.16143 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15148.69 15229.76 26.45 0.16160 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/216.ctf NX, NY, NZ: 512 512 1 MODE : real