CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/215.mrc Output diagnostic file name Micrographs_0601/215.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/215.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2408.000 9608.000 Mean, RMS : 4507.127 217.0519 TITLE 1: emi2mrc: 215.emi Sun Jan 05 17:45:02 2014 TITLE 2: kV 300, Mag 78000x, Def 25760, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 47.664, Y 525.137, Z -81.406, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01936 10200.00 10000.00 0.00 0.02039 10400.00 10000.00 0.00 0.02124 10600.00 10000.00 0.00 0.02233 10800.00 10000.00 0.00 0.02343 11000.00 10000.00 0.00 0.02468 11200.00 10000.00 0.00 0.02612 11400.00 10000.00 0.00 0.02763 11600.00 10000.00 0.00 0.02917 11800.00 10000.00 0.00 0.03082 12000.00 10000.00 0.00 0.03257 12200.00 10000.00 0.00 0.03426 12400.00 10000.00 0.00 0.03625 12600.00 10000.00 0.00 0.03825 12800.00 10000.00 0.00 0.04028 13000.00 10000.00 0.00 0.04247 13200.00 10000.00 0.00 0.04458 13400.00 10000.00 0.00 0.04670 13600.00 10000.00 0.00 0.04898 13800.00 10000.00 0.00 0.05144 14000.00 10000.00 0.00 0.05380 14200.00 10000.00 0.00 0.05635 14400.00 10000.00 0.00 0.05897 14600.00 10000.00 0.00 0.06155 14800.00 10000.00 0.00 0.06427 15000.00 10000.00 0.00 0.06728 15200.00 10000.00 0.00 0.07078 15400.00 10000.00 0.00 0.07435 15600.00 10000.00 0.00 0.07811 15800.00 10000.00 0.00 0.08113 16000.00 10000.00 0.00 0.08245 16200.00 10000.00 0.00 0.08281 16400.00 10000.00 0.00 0.08292 16000.00 10200.00 0.00 0.08410 16200.00 10200.00 0.00 0.08440 16400.00 10200.00 0.00 0.08441 16000.00 10400.00 0.00 0.08567 16200.00 10400.00 0.00 0.08588 15800.00 10600.00 0.00 0.08599 16000.00 10600.00 0.00 0.08722 16200.00 10600.00 0.00 0.08735 15800.00 10800.00 0.00 0.08762 16000.00 10800.00 0.00 0.08878 16200.00 10800.00 0.00 0.08885 15800.00 11000.00 0.00 0.08941 16000.00 11000.00 0.00 0.09051 16200.00 11000.00 0.00 0.09054 15800.00 11200.00 0.00 0.09118 16000.00 11200.00 0.00 0.09224 16200.00 11200.00 0.00 0.09225 15800.00 11400.00 0.00 0.09314 16000.00 11400.00 0.00 0.09415 16200.00 11400.00 0.00 0.09415 15800.00 11600.00 0.00 0.09522 16000.00 11600.00 0.00 0.09624 16200.00 11600.00 0.00 0.09629 15800.00 11800.00 0.00 0.09760 16000.00 11800.00 0.00 0.09866 16200.00 11800.00 0.00 0.09871 15800.00 12000.00 0.00 0.10016 16000.00 12000.00 0.00 0.10117 15800.00 12200.00 0.00 0.10268 16000.00 12200.00 0.00 0.10359 15800.00 12400.00 0.00 0.10524 16000.00 12400.00 0.00 0.10605 15800.00 12600.00 0.00 0.10777 16000.00 12600.00 0.00 0.10853 15800.00 12800.00 0.00 0.11048 16000.00 12800.00 0.00 0.11123 15800.00 13000.00 0.00 0.11322 16000.00 13000.00 0.00 0.11398 15800.00 13200.00 0.00 0.11637 16000.00 13200.00 0.00 0.11708 15800.00 13400.00 0.00 0.11965 16000.00 13400.00 0.00 0.12025 15800.00 13600.00 0.00 0.12308 16000.00 13600.00 0.00 0.12359 15800.00 13800.00 0.00 0.12666 16000.00 13800.00 0.00 0.12701 15800.00 14000.00 0.00 0.13012 16000.00 14000.00 0.00 0.13028 15600.00 14200.00 0.00 0.13085 15800.00 14200.00 0.00 0.13389 15600.00 14400.00 0.00 0.13473 15800.00 14400.00 0.00 0.13770 15600.00 14600.00 0.00 0.13898 15800.00 14600.00 0.00 0.14179 15600.00 14800.00 0.00 0.14301 15800.00 14800.00 0.00 0.14563 15600.00 15000.00 0.00 0.14729 15800.00 15000.00 0.00 0.14965 15600.00 15200.00 0.00 0.15142 15800.00 15200.00 0.00 0.15336 15600.00 15400.00 0.00 0.15527 15800.00 15400.00 0.00 0.15661 15600.00 15600.00 0.00 0.15949 15800.00 15600.00 0.00 0.15955 15600.00 15800.00 0.00 0.16067 15600.00 15800.00 5.00 0.16083 15600.00 15800.00 10.00 0.16098 15600.00 15800.00 15.00 0.16110 15600.00 15800.00 20.00 0.16119 15600.00 15800.00 25.00 0.16126 15600.00 15800.00 30.00 0.16128 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15634.46 15765.42 30.00 0.16140 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/215.ctf NX, NY, NZ: 512 512 1 MODE : real