CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/214.mrc Output diagnostic file name Micrographs_0601/214.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/214.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2352.000 8564.000 Mean, RMS : 4510.572 216.5089 TITLE 1: emi2mrc: 214.emi Sun Jan 05 17:44:08 2014 TITLE 2: kV 300, Mag 78000x, Def 25760, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 45.195, Y 525.573, Z -81.406, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01370 10200.00 10000.00 0.00 0.01430 10400.00 10000.00 0.00 0.01502 10600.00 10000.00 0.00 0.01594 10800.00 10000.00 0.00 0.01665 11000.00 10000.00 0.00 0.01747 11200.00 10000.00 0.00 0.01844 11400.00 10000.00 0.00 0.01946 11600.00 10000.00 0.00 0.02059 11800.00 10000.00 0.00 0.02175 12000.00 10000.00 0.00 0.02312 12200.00 10000.00 0.00 0.02462 12400.00 10000.00 0.00 0.02631 12600.00 10000.00 0.00 0.02803 12800.00 10000.00 0.00 0.02963 13000.00 10000.00 0.00 0.03132 13200.00 10000.00 0.00 0.03316 13400.00 10000.00 0.00 0.03503 13600.00 10000.00 0.00 0.03700 13800.00 10000.00 0.00 0.03918 14000.00 10000.00 0.00 0.04138 14200.00 10000.00 0.00 0.04365 14400.00 10000.00 0.00 0.04589 14600.00 10000.00 0.00 0.04806 14800.00 10000.00 0.00 0.05019 15000.00 10000.00 0.00 0.05224 15200.00 10000.00 0.00 0.05435 15400.00 10000.00 0.00 0.05676 15600.00 10000.00 0.00 0.05947 15800.00 10000.00 0.00 0.06219 16000.00 10000.00 0.00 0.06514 16200.00 10000.00 0.00 0.06829 16400.00 10000.00 0.00 0.07168 16600.00 10000.00 0.00 0.07477 16800.00 10000.00 0.00 0.07613 17000.00 10000.00 0.00 0.07636 16800.00 10200.00 0.00 0.07740 17000.00 10200.00 0.00 0.07764 16800.00 10400.00 0.00 0.07866 17000.00 10400.00 0.00 0.07887 16800.00 10600.00 0.00 0.07986 17000.00 10600.00 0.00 0.08006 16800.00 10800.00 0.00 0.08120 17000.00 10800.00 0.00 0.08140 16600.00 11000.00 0.00 0.08145 16800.00 11000.00 0.00 0.08274 17000.00 11000.00 0.00 0.08289 16800.00 11200.00 0.00 0.08412 17000.00 11200.00 0.00 0.08422 16600.00 11400.00 0.00 0.08444 16800.00 11400.00 0.00 0.08568 17000.00 11400.00 0.00 0.08580 16600.00 11600.00 0.00 0.08606 16800.00 11600.00 0.00 0.08733 17000.00 11600.00 0.00 0.08747 16600.00 11800.00 0.00 0.08774 16800.00 11800.00 0.00 0.08901 17000.00 11800.00 0.00 0.08915 16600.00 12000.00 0.00 0.08941 16800.00 12000.00 0.00 0.09073 17000.00 12000.00 0.00 0.09088 16600.00 12200.00 0.00 0.09129 16800.00 12200.00 0.00 0.09266 17000.00 12200.00 0.00 0.09280 16600.00 12400.00 0.00 0.09339 16800.00 12400.00 0.00 0.09476 17000.00 12400.00 0.00 0.09481 16600.00 12600.00 0.00 0.09541 16800.00 12600.00 0.00 0.09677 16600.00 12800.00 0.00 0.09766 16800.00 12800.00 0.00 0.09901 16600.00 13000.00 0.00 0.09997 16800.00 13000.00 0.00 0.10128 16600.00 13200.00 0.00 0.10263 16800.00 13200.00 0.00 0.10390 16600.00 13400.00 0.00 0.10522 16800.00 13400.00 0.00 0.10643 16600.00 13600.00 0.00 0.10780 16800.00 13600.00 0.00 0.10897 16600.00 13800.00 0.00 0.11066 16800.00 13800.00 0.00 0.11177 16600.00 14000.00 0.00 0.11344 16800.00 14000.00 0.00 0.11445 16600.00 14200.00 0.00 0.11650 16800.00 14200.00 0.00 0.11739 16600.00 14400.00 0.00 0.11952 16800.00 14400.00 0.00 0.12024 16600.00 14600.00 0.00 0.12263 16800.00 14600.00 0.00 0.12319 16600.00 14800.00 0.00 0.12554 16800.00 14800.00 0.00 0.12597 16600.00 15000.00 0.00 0.12841 16800.00 15000.00 0.00 0.12870 16600.00 15200.00 0.00 0.13135 16800.00 15200.00 0.00 0.13149 16600.00 15400.00 0.00 0.13426 16400.00 15600.00 0.00 0.13437 16600.00 15600.00 0.00 0.13743 16600.00 15800.00 0.00 0.14026 16400.00 16000.00 0.00 0.14031 16600.00 16000.00 0.00 0.14303 16600.00 16200.00 0.00 0.14536 16400.00 16400.00 0.00 0.14554 16600.00 16400.00 0.00 0.14764 16400.00 16600.00 0.00 0.14769 16600.00 16600.00 0.00 0.14864 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 16514.64 16616.21 24.05 0.14907 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/214.ctf NX, NY, NZ: 512 512 1 MODE : real