CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/213.mrc Output diagnostic file name Micrographs_0601/213.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/213.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2186.000 7642.000 Mean, RMS : 4505.526 222.4593 TITLE 1: emi2mrc: 213.emi Sun Jan 05 17:39:02 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 32.225, Y 527.164, Z -81.867, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01790 15000.00 10000.00 0.00 -0.01789 15200.00 10000.00 0.00 -0.01766 15400.00 10000.00 0.00 -0.01749 15600.00 10000.00 0.00 -0.01737 15800.00 10000.00 0.00 -0.01709 16000.00 10000.00 0.00 -0.01673 16200.00 10000.00 0.00 -0.01617 16400.00 10000.00 0.00 -0.01563 16600.00 10000.00 0.00 -0.01520 16800.00 10000.00 0.00 -0.01464 17000.00 10000.00 0.00 -0.01404 17200.00 10000.00 0.00 -0.01352 17400.00 10000.00 0.00 -0.01301 17600.00 10000.00 0.00 -0.01233 17800.00 10000.00 0.00 -0.01163 18000.00 10000.00 0.00 -0.01094 18200.00 10000.00 0.00 -0.01023 18400.00 10000.00 0.00 -0.00960 18600.00 10000.00 0.00 -0.00903 18800.00 10000.00 0.00 -0.00861 19000.00 10000.00 0.00 -0.00821 19200.00 10000.00 0.00 -0.00775 19400.00 10000.00 0.00 -0.00722 19600.00 10000.00 0.00 -0.00656 19800.00 10000.00 0.00 -0.00588 20000.00 10000.00 0.00 -0.00514 20200.00 10000.00 0.00 -0.00427 20400.00 10000.00 0.00 -0.00326 20600.00 10000.00 0.00 -0.00215 20800.00 10000.00 0.00 -0.00104 21000.00 10000.00 0.00 -0.00002 21200.00 10000.00 0.00 0.00095 21400.00 10000.00 0.00 0.00201 21600.00 10000.00 0.00 0.00318 21800.00 10000.00 0.00 0.00423 22000.00 10000.00 0.00 0.00503 22200.00 10000.00 0.00 0.00588 22400.00 10000.00 0.00 0.00690 22600.00 10000.00 0.00 0.00784 22800.00 10000.00 0.00 0.00883 23000.00 10000.00 0.00 0.00997 23200.00 10000.00 0.00 0.01112 23400.00 10000.00 0.00 0.01231 23600.00 10000.00 0.00 0.01350 23800.00 10000.00 0.00 0.01478 24000.00 10000.00 0.00 0.01615 24200.00 10000.00 0.00 0.01752 24400.00 10000.00 0.00 0.01893 24600.00 10000.00 0.00 0.02037 24800.00 10000.00 0.00 0.02193 25000.00 10000.00 0.00 0.02370 25200.00 10000.00 0.00 0.02548 25400.00 10000.00 0.00 0.02672 25600.00 10000.00 0.00 0.02734 25800.00 10000.00 0.00 0.02772 26000.00 10000.00 0.00 0.02798 26200.00 10000.00 0.00 0.02817 26000.00 10200.00 0.00 0.02823 26200.00 10200.00 0.00 0.02839 26000.00 10400.00 0.00 0.02839 26200.00 10400.00 0.00 0.02855 26000.00 10600.00 0.00 0.02866 26200.00 10600.00 0.00 0.02886 26400.00 10600.00 0.00 0.02888 25800.00 10800.00 0.00 0.02893 26000.00 10800.00 0.00 0.02915 26200.00 10800.00 0.00 0.02940 26400.00 10800.00 0.00 0.02942 25800.00 11000.00 0.00 0.02946 26000.00 11000.00 0.00 0.02973 26200.00 11000.00 0.00 0.02999 26000.00 11200.00 0.00 0.03019 26200.00 11200.00 0.00 0.03044 26000.00 11400.00 0.00 0.03073 26200.00 11400.00 0.00 0.03095 26000.00 11600.00 0.00 0.03130 26200.00 11600.00 0.00 0.03145 25800.00 11800.00 0.00 0.03149 26000.00 11800.00 0.00 0.03181 26200.00 11800.00 0.00 0.03187 25800.00 12000.00 0.00 0.03204 26000.00 12000.00 0.00 0.03226 25800.00 12200.00 0.00 0.03245 26000.00 12200.00 0.00 0.03263 26200.00 12200.00 0.00 0.03264 25800.00 12400.00 0.00 0.03294 26000.00 12400.00 0.00 0.03312 26200.00 12400.00 0.00 0.03319 25800.00 12600.00 0.00 0.03353 26000.00 12600.00 0.00 0.03369 26200.00 12600.00 0.00 0.03380 25800.00 12800.00 0.00 0.03403 26000.00 12800.00 0.00 0.03419 26200.00 12800.00 0.00 0.03432 25800.00 13000.00 0.00 0.03450 26000.00 13000.00 0.00 0.03469 26200.00 13000.00 0.00 0.03484 25800.00 13200.00 0.00 0.03505 26000.00 13200.00 0.00 0.03525 26200.00 13200.00 0.00 0.03535 25800.00 13400.00 0.00 0.03555 26000.00 13400.00 0.00 0.03571 26200.00 13400.00 0.00 0.03575 25800.00 13600.00 0.00 0.03601 26000.00 13600.00 0.00 0.03619 26200.00 13600.00 0.00 0.03623 25600.00 13800.00 0.00 0.03626 25800.00 13800.00 0.00 0.03656 26000.00 13800.00 0.00 0.03677 26200.00 13800.00 0.00 0.03678 25800.00 14000.00 0.00 0.03703 26000.00 14000.00 0.00 0.03722 25800.00 14200.00 0.00 0.03744 26000.00 14200.00 0.00 0.03760 25800.00 14400.00 0.00 0.03794 26000.00 14400.00 0.00 0.03809 25600.00 14600.00 0.00 0.03817 25800.00 14600.00 0.00 0.03856 26000.00 14600.00 0.00 0.03866 25600.00 14800.00 0.00 0.03885 25800.00 14800.00 0.00 0.03920 26000.00 14800.00 0.00 0.03920 25600.00 15000.00 0.00 0.03952 25800.00 15000.00 0.00 0.03978 25600.00 15200.00 0.00 0.04014 25800.00 15200.00 0.00 0.04034 25600.00 15400.00 0.00 0.04084 25800.00 15400.00 0.00 0.04103 25600.00 15600.00 0.00 0.04162 25800.00 15600.00 0.00 0.04179 25400.00 15800.00 0.00 0.04187 25600.00 15800.00 0.00 0.04248 25800.00 15800.00 0.00 0.04261 25400.00 16000.00 0.00 0.04287 25600.00 16000.00 0.00 0.04344 25800.00 16000.00 0.00 0.04353 25400.00 16200.00 0.00 0.04381 25600.00 16200.00 0.00 0.04435 25800.00 16200.00 0.00 0.04441 26000.00 16200.00 0.00 0.04443 26200.00 16200.00 0.00 0.04443 25400.00 16400.00 0.00 0.04495 25600.00 16400.00 0.00 0.04546 25800.00 16400.00 0.00 0.04555 26000.00 16400.00 0.00 0.04565 26200.00 16400.00 0.00 0.04568 25400.00 16600.00 0.00 0.04619 25600.00 16600.00 0.00 0.04669 25800.00 16600.00 0.00 0.04685 26000.00 16600.00 0.00 0.04700 25400.00 16800.00 0.00 0.04742 25600.00 16800.00 0.00 0.04794 25800.00 16800.00 0.00 0.04814 26000.00 16800.00 0.00 0.04829 25400.00 17000.00 0.00 0.04854 25600.00 17000.00 0.00 0.04907 25800.00 17000.00 0.00 0.04928 26000.00 17000.00 0.00 0.04936 25400.00 17200.00 0.00 0.04951 25600.00 17200.00 0.00 0.05004 25800.00 17200.00 0.00 0.05022 26000.00 17200.00 0.00 0.05023 25400.00 17400.00 0.00 0.05054 25600.00 17400.00 0.00 0.05107 25800.00 17400.00 0.00 0.05123 25400.00 17600.00 0.00 0.05158 25600.00 17600.00 0.00 0.05212 25800.00 17600.00 0.00 0.05226 25400.00 17800.00 0.00 0.05275 25600.00 17800.00 0.00 0.05326 25800.00 17800.00 0.00 0.05336 25400.00 18000.00 0.00 0.05388 25600.00 18000.00 0.00 0.05435 25800.00 18000.00 0.00 0.05442 25400.00 18200.00 0.00 0.05503 25600.00 18200.00 0.00 0.05549 25800.00 18200.00 0.00 0.05557 25400.00 18400.00 0.00 0.05621 25600.00 18400.00 0.00 0.05668 25800.00 18400.00 0.00 0.05675 25400.00 18600.00 0.00 0.05740 25600.00 18600.00 0.00 0.05788 25800.00 18600.00 0.00 0.05791 25400.00 18800.00 0.00 0.05874 25600.00 18800.00 0.00 0.05919 25400.00 19000.00 0.00 0.05995 25600.00 19000.00 0.00 0.06039 25400.00 19200.00 0.00 0.06141 25600.00 19200.00 0.00 0.06181 25400.00 19400.00 0.00 0.06287 25600.00 19400.00 0.00 0.06323 25400.00 19600.00 0.00 0.06417 25600.00 19600.00 0.00 0.06450 25400.00 19800.00 0.00 0.06557 25600.00 19800.00 0.00 0.06588 25400.00 20000.00 0.00 0.06703 25600.00 20000.00 0.00 0.06732 25400.00 20200.00 0.00 0.06856 25600.00 20200.00 0.00 0.06882 25400.00 20400.00 0.00 0.06995 25600.00 20400.00 0.00 0.07014 25400.00 20600.00 0.00 0.07131 25600.00 20600.00 0.00 0.07143 25200.00 20800.00 0.00 0.07152 25400.00 20800.00 0.00 0.07274 25600.00 20800.00 0.00 0.07282 25200.00 21000.00 0.00 0.07291 25400.00 21000.00 0.00 0.07414 25600.00 21000.00 0.00 0.07424 25200.00 21200.00 0.00 0.07436 25400.00 21200.00 0.00 0.07562 25600.00 21200.00 0.00 0.07572 25200.00 21400.00 0.00 0.07596 25400.00 21400.00 0.00 0.07723 25600.00 21400.00 0.00 0.07731 25200.00 21600.00 0.00 0.07771 25400.00 21600.00 0.00 0.07897 25600.00 21600.00 0.00 0.07902 25200.00 21800.00 0.00 0.07959 25400.00 21800.00 0.00 0.08080 25200.00 22000.00 0.00 0.08136 25400.00 22000.00 0.00 0.08252 25200.00 22200.00 0.00 0.08312 25400.00 22200.00 0.00 0.08425 25200.00 22400.00 0.00 0.08513 25400.00 22400.00 0.00 0.08622 25200.00 22600.00 0.00 0.08712 25400.00 22600.00 0.00 0.08812 25200.00 22800.00 0.00 0.08918 25400.00 22800.00 0.00 0.09013 25200.00 23000.00 0.00 0.09123 25400.00 23000.00 0.00 0.09217 25200.00 23200.00 0.00 0.09332 25400.00 23200.00 0.00 0.09424 25200.00 23400.00 0.00 0.09559 25400.00 23400.00 0.00 0.09644 25200.00 23600.00 0.00 0.09783 25400.00 23600.00 0.00 0.09858 25200.00 23800.00 0.00 0.10012 25400.00 23800.00 0.00 0.10074 25200.00 24000.00 0.00 0.10243 25400.00 24000.00 0.00 0.10288 25200.00 24200.00 0.00 0.10500 25400.00 24200.00 0.00 0.10524 25200.00 24400.00 0.00 0.10744 25000.00 24600.00 0.00 0.10773 25200.00 24600.00 0.00 0.10977 25000.00 24800.00 0.00 0.11007 25200.00 24800.00 0.00 0.11182 25000.00 25000.00 0.00 0.11191 25200.00 25000.00 0.00 0.11335 25000.00 25200.00 0.00 0.11342 25200.00 25200.00 0.00 0.11429 25000.00 25400.00 20.00 0.11433 25000.00 25400.00 25.00 0.11451 25000.00 25400.00 30.00 0.11464 25000.00 25400.00 35.00 0.11469 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25078.87 25326.44 36.13 0.11498 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/213.ctf NX, NY, NZ: 512 512 1 MODE : real