CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/211.mrc Output diagnostic file name Micrographs_0601/211.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/211.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2266.000 7762.000 Mean, RMS : 4506.381 214.5133 TITLE 1: emi2mrc: 211.emi Sun Jan 05 17:41:13 2014 TITLE 2: kV 300, Mag 78000x, Def 25760, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 34.776, Y 527.041, Z -81.406, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00223 10200.00 10000.00 0.00 -0.00212 10400.00 10000.00 0.00 -0.00181 10600.00 10000.00 0.00 -0.00142 10800.00 10000.00 0.00 -0.00108 11000.00 10000.00 0.00 -0.00065 11200.00 10000.00 0.00 -0.00012 11400.00 10000.00 0.00 0.00036 11600.00 10000.00 0.00 0.00077 11800.00 10000.00 0.00 0.00124 12000.00 10000.00 0.00 0.00164 12200.00 10000.00 0.00 0.00192 12400.00 10000.00 0.00 0.00231 12600.00 10000.00 0.00 0.00285 12800.00 10000.00 0.00 0.00347 13000.00 10000.00 0.00 0.00401 13200.00 10000.00 0.00 0.00462 13400.00 10000.00 0.00 0.00530 13600.00 10000.00 0.00 0.00577 13800.00 10000.00 0.00 0.00635 14000.00 10000.00 0.00 0.00704 14200.00 10000.00 0.00 0.00776 14400.00 10000.00 0.00 0.00876 14600.00 10000.00 0.00 0.00970 14800.00 10000.00 0.00 0.01056 15000.00 10000.00 0.00 0.01167 15200.00 10000.00 0.00 0.01280 15400.00 10000.00 0.00 0.01386 15600.00 10000.00 0.00 0.01497 15800.00 10000.00 0.00 0.01614 16000.00 10000.00 0.00 0.01743 16200.00 10000.00 0.00 0.01864 16400.00 10000.00 0.00 0.01988 16600.00 10000.00 0.00 0.02124 16800.00 10000.00 0.00 0.02240 17000.00 10000.00 0.00 0.02361 17200.00 10000.00 0.00 0.02498 17400.00 10000.00 0.00 0.02636 17600.00 10000.00 0.00 0.02775 17800.00 10000.00 0.00 0.02933 18000.00 10000.00 0.00 0.03091 18200.00 10000.00 0.00 0.03211 18400.00 10000.00 0.00 0.03329 18600.00 10000.00 0.00 0.03467 18800.00 10000.00 0.00 0.03626 19000.00 10000.00 0.00 0.03808 19200.00 10000.00 0.00 0.04004 19400.00 10000.00 0.00 0.04211 19600.00 10000.00 0.00 0.04431 19800.00 10000.00 0.00 0.04660 20000.00 10000.00 0.00 0.04876 20200.00 10000.00 0.00 0.05052 20400.00 10000.00 0.00 0.05160 20600.00 10000.00 0.00 0.05209 20800.00 10000.00 0.00 0.05219 20600.00 10200.00 0.00 0.05250 20800.00 10200.00 0.00 0.05260 20600.00 10400.00 0.00 0.05299 20800.00 10400.00 0.00 0.05308 20400.00 10600.00 0.00 0.05325 20600.00 10600.00 0.00 0.05370 20800.00 10600.00 0.00 0.05379 20400.00 10800.00 0.00 0.05399 20600.00 10800.00 0.00 0.05443 20800.00 10800.00 0.00 0.05456 20400.00 11000.00 0.00 0.05469 20600.00 11000.00 0.00 0.05514 20800.00 11000.00 0.00 0.05532 20400.00 11200.00 0.00 0.05544 20600.00 11200.00 0.00 0.05591 20800.00 11200.00 0.00 0.05613 20400.00 11400.00 0.00 0.05648 20600.00 11400.00 0.00 0.05696 20800.00 11400.00 0.00 0.05717 20400.00 11600.00 0.00 0.05747 20600.00 11600.00 0.00 0.05790 20800.00 11600.00 0.00 0.05804 20400.00 11800.00 0.00 0.05839 20600.00 11800.00 0.00 0.05876 20800.00 11800.00 0.00 0.05884 20400.00 12000.00 0.00 0.05926 20600.00 12000.00 0.00 0.05959 20800.00 12000.00 0.00 0.05968 20400.00 12200.00 0.00 0.06008 20600.00 12200.00 0.00 0.06044 20800.00 12200.00 0.00 0.06055 20400.00 12400.00 0.00 0.06110 20600.00 12400.00 0.00 0.06149 20800.00 12400.00 0.00 0.06159 20400.00 12600.00 0.00 0.06215 20600.00 12600.00 0.00 0.06249 20800.00 12600.00 0.00 0.06254 20400.00 12800.00 0.00 0.06322 20600.00 12800.00 0.00 0.06351 20800.00 12800.00 0.00 0.06353 20400.00 13000.00 0.00 0.06429 20600.00 13000.00 0.00 0.06456 20800.00 13000.00 0.00 0.06460 20400.00 13200.00 0.00 0.06540 20600.00 13200.00 0.00 0.06567 20800.00 13200.00 0.00 0.06576 20400.00 13400.00 0.00 0.06660 20600.00 13400.00 0.00 0.06689 20800.00 13400.00 0.00 0.06702 20400.00 13600.00 0.00 0.06796 20600.00 13600.00 0.00 0.06824 20800.00 13600.00 0.00 0.06838 20400.00 13800.00 0.00 0.06923 20600.00 13800.00 0.00 0.06949 20800.00 13800.00 0.00 0.06960 20400.00 14000.00 0.00 0.07022 20600.00 14000.00 0.00 0.07045 20800.00 14000.00 0.00 0.07052 20400.00 14200.00 0.00 0.07124 20600.00 14200.00 0.00 0.07146 20800.00 14200.00 0.00 0.07151 20400.00 14400.00 0.00 0.07235 20600.00 14400.00 0.00 0.07258 20800.00 14400.00 0.00 0.07265 20200.00 14600.00 0.00 0.07268 20400.00 14600.00 0.00 0.07359 20600.00 14600.00 0.00 0.07382 20800.00 14600.00 0.00 0.07390 20200.00 14800.00 0.00 0.07393 20400.00 14800.00 0.00 0.07481 20600.00 14800.00 0.00 0.07509 20800.00 14800.00 0.00 0.07519 20200.00 15000.00 0.00 0.07535 20400.00 15000.00 0.00 0.07627 20600.00 15000.00 0.00 0.07662 20800.00 15000.00 0.00 0.07676 20200.00 15200.00 0.00 0.07699 20400.00 15200.00 0.00 0.07796 20600.00 15200.00 0.00 0.07836 20800.00 15200.00 0.00 0.07848 20200.00 15400.00 0.00 0.07880 20400.00 15400.00 0.00 0.07982 20600.00 15400.00 0.00 0.08023 20800.00 15400.00 0.00 0.08030 20200.00 15600.00 0.00 0.08075 20400.00 15600.00 0.00 0.08180 20600.00 15600.00 0.00 0.08220 20800.00 15600.00 0.00 0.08222 20200.00 15800.00 0.00 0.08272 20400.00 15800.00 0.00 0.08376 20600.00 15800.00 0.00 0.08413 20200.00 16000.00 0.00 0.08484 20400.00 16000.00 0.00 0.08587 20600.00 16000.00 0.00 0.08618 20200.00 16200.00 0.00 0.08675 20400.00 16200.00 0.00 0.08780 20600.00 16200.00 0.00 0.08806 20200.00 16400.00 0.00 0.08878 20400.00 16400.00 0.00 0.08982 20600.00 16400.00 0.00 0.09001 20200.00 16600.00 0.00 0.09077 20400.00 16600.00 0.00 0.09176 20600.00 16600.00 0.00 0.09186 20200.00 16800.00 0.00 0.09267 20400.00 16800.00 0.00 0.09359 20600.00 16800.00 0.00 0.09367 20200.00 17000.00 0.00 0.09476 20400.00 17000.00 0.00 0.09565 20600.00 17000.00 0.00 0.09572 20200.00 17200.00 0.00 0.09681 20400.00 17200.00 0.00 0.09765 20600.00 17200.00 0.00 0.09771 20200.00 17400.00 0.00 0.09916 20400.00 17400.00 0.00 0.09993 20600.00 17400.00 0.00 0.09996 20200.00 17600.00 0.00 0.10150 20400.00 17600.00 0.00 0.10219 20600.00 17600.00 0.00 0.10220 20200.00 17800.00 0.00 0.10376 20400.00 17800.00 0.00 0.10439 20600.00 17800.00 0.00 0.10439 20200.00 18000.00 0.00 0.10606 20400.00 18000.00 0.00 0.10669 20600.00 18000.00 0.00 0.10671 20000.00 18200.00 0.00 0.10673 20200.00 18200.00 0.00 0.10879 20400.00 18200.00 0.00 0.10948 20600.00 18200.00 0.00 0.10950 20000.00 18400.00 0.00 0.10979 20200.00 18400.00 0.00 0.11187 20400.00 18400.00 0.00 0.11259 20000.00 18600.00 0.00 0.11278 20200.00 18600.00 0.00 0.11489 20400.00 18600.00 0.00 0.11559 20000.00 18800.00 0.00 0.11588 20200.00 18800.00 0.00 0.11805 20400.00 18800.00 0.00 0.11867 20000.00 19000.00 0.00 0.11898 20200.00 19000.00 0.00 0.12116 20400.00 19000.00 0.00 0.12162 20000.00 19200.00 0.00 0.12236 20200.00 19200.00 0.00 0.12447 20400.00 19200.00 0.00 0.12466 20000.00 19400.00 0.00 0.12586 20200.00 19400.00 0.00 0.12788 20000.00 19600.00 0.00 0.12978 20200.00 19600.00 0.00 0.13165 20000.00 19800.00 0.00 0.13367 20200.00 19800.00 0.00 0.13532 20000.00 20000.00 0.00 0.13719 20200.00 20000.00 0.00 0.13841 20000.00 20200.00 0.00 0.13907 20200.00 20200.00 0.00 0.13926 20000.00 20200.00 10.00 0.13941 20000.00 20200.00 15.00 0.13956 20000.00 20200.00 20.00 0.13967 20000.00 20200.00 25.00 0.13976 20000.00 20200.00 30.00 0.13981 20000.00 20200.00 35.00 0.13983 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20059.90 20226.64 37.82 0.14007 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/211.ctf NX, NY, NZ: 512 512 1 MODE : real