CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/208.mrc Output diagnostic file name Micrographs_0601/208.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/208.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2272.000 8702.000 Mean, RMS : 4512.178 222.1889 TITLE 1: emi2mrc: 208.emi Sun Jan 05 17:35:23 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 37.314, Y 529.428, Z -81.867, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02000 18000.00 10000.00 0.00 -0.01980 18200.00 10000.00 0.00 -0.01934 18400.00 10000.00 0.00 -0.01886 18600.00 10000.00 0.00 -0.01828 18800.00 10000.00 0.00 -0.01762 19000.00 10000.00 0.00 -0.01701 19200.00 10000.00 0.00 -0.01633 19400.00 10000.00 0.00 -0.01557 19600.00 10000.00 0.00 -0.01477 19800.00 10000.00 0.00 -0.01407 20000.00 10000.00 0.00 -0.01343 20200.00 10000.00 0.00 -0.01268 20400.00 10000.00 0.00 -0.01178 20600.00 10000.00 0.00 -0.01085 20800.00 10000.00 0.00 -0.00999 21000.00 10000.00 0.00 -0.00922 21200.00 10000.00 0.00 -0.00848 21400.00 10000.00 0.00 -0.00780 21600.00 10000.00 0.00 -0.00712 21800.00 10000.00 0.00 -0.00631 22000.00 10000.00 0.00 -0.00544 22200.00 10000.00 0.00 -0.00481 22400.00 10000.00 0.00 -0.00425 22600.00 10000.00 0.00 -0.00347 22800.00 10000.00 0.00 -0.00278 23000.00 10000.00 0.00 -0.00216 23200.00 10000.00 0.00 -0.00126 23400.00 10000.00 0.00 -0.00034 23600.00 10000.00 0.00 0.00055 23800.00 10000.00 0.00 0.00154 24000.00 10000.00 0.00 0.00250 24200.00 10000.00 0.00 0.00362 24400.00 10000.00 0.00 0.00485 24600.00 10000.00 0.00 0.00597 24800.00 10000.00 0.00 0.00718 25000.00 10000.00 0.00 0.00833 25200.00 10000.00 0.00 0.00944 25400.00 10000.00 0.00 0.01088 25600.00 10000.00 0.00 0.01256 25800.00 10000.00 0.00 0.01431 26000.00 10000.00 0.00 0.01609 26200.00 10000.00 0.00 0.01793 26400.00 10000.00 0.00 0.01978 26600.00 10000.00 0.00 0.02120 26800.00 10000.00 0.00 0.02194 27000.00 10000.00 0.00 0.02223 27000.00 10200.00 0.00 0.02232 27200.00 10200.00 0.00 0.02233 27000.00 10400.00 0.00 0.02251 27000.00 10600.00 0.00 0.02271 27000.00 10800.00 0.00 0.02293 27000.00 11000.00 0.00 0.02316 27000.00 11200.00 0.00 0.02326 27000.00 11600.00 0.00 0.02333 27200.00 11600.00 0.00 0.02333 27000.00 11800.00 0.00 0.02349 27200.00 11800.00 0.00 0.02355 27000.00 12000.00 0.00 0.02373 27200.00 12000.00 0.00 0.02384 27000.00 12200.00 0.00 0.02409 27200.00 12200.00 0.00 0.02418 27000.00 12400.00 0.00 0.02442 27200.00 12400.00 0.00 0.02446 27000.00 12600.00 0.00 0.02479 27200.00 12600.00 0.00 0.02481 27000.00 12800.00 0.00 0.02526 27000.00 13000.00 0.00 0.02568 27000.00 13200.00 0.00 0.02611 27200.00 13200.00 0.00 0.02613 27000.00 13400.00 0.00 0.02654 27200.00 13400.00 0.00 0.02656 26800.00 13600.00 0.00 0.02658 27000.00 13600.00 0.00 0.02700 26800.00 13800.00 0.00 0.02703 27000.00 13800.00 0.00 0.02739 27000.00 14000.00 0.00 0.02763 27000.00 14200.00 0.00 0.02786 26800.00 14400.00 0.00 0.02790 27000.00 14400.00 0.00 0.02817 26800.00 14600.00 0.00 0.02831 27000.00 14600.00 0.00 0.02859 26800.00 14800.00 0.00 0.02876 27000.00 14800.00 0.00 0.02903 26800.00 15000.00 0.00 0.02925 27000.00 15000.00 0.00 0.02950 26800.00 15200.00 0.00 0.02974 27000.00 15200.00 0.00 0.02997 26800.00 15400.00 0.00 0.03031 27000.00 15400.00 0.00 0.03053 26800.00 15600.00 0.00 0.03087 27000.00 15600.00 0.00 0.03107 26800.00 15800.00 0.00 0.03136 27000.00 15800.00 0.00 0.03155 26800.00 16000.00 0.00 0.03198 27000.00 16000.00 0.00 0.03217 26800.00 16200.00 0.00 0.03263 27000.00 16200.00 0.00 0.03283 26800.00 16400.00 0.00 0.03336 27000.00 16400.00 0.00 0.03353 26800.00 16600.00 0.00 0.03406 27000.00 16600.00 0.00 0.03417 26800.00 16800.00 0.00 0.03465 27000.00 16800.00 0.00 0.03475 26800.00 17000.00 0.00 0.03538 27000.00 17000.00 0.00 0.03552 26800.00 17200.00 0.00 0.03623 27000.00 17200.00 0.00 0.03637 26800.00 17400.00 0.00 0.03703 27000.00 17400.00 0.00 0.03711 26800.00 17600.00 0.00 0.03778 27000.00 17600.00 0.00 0.03782 26600.00 17800.00 0.00 0.03793 26800.00 17800.00 0.00 0.03868 27000.00 17800.00 0.00 0.03873 26600.00 18000.00 0.00 0.03889 26800.00 18000.00 0.00 0.03963 27000.00 18000.00 0.00 0.03965 26600.00 18200.00 0.00 0.03981 26800.00 18200.00 0.00 0.04052 26600.00 18400.00 0.00 0.04072 26800.00 18400.00 0.00 0.04140 26600.00 18600.00 0.00 0.04161 26800.00 18600.00 0.00 0.04228 26600.00 18800.00 0.00 0.04261 26800.00 18800.00 0.00 0.04329 26600.00 19000.00 0.00 0.04387 26800.00 19000.00 0.00 0.04455 27000.00 19000.00 0.00 0.04455 26600.00 19200.00 0.00 0.04509 26800.00 19200.00 0.00 0.04574 27000.00 19200.00 0.00 0.04576 26600.00 19400.00 0.00 0.04620 26800.00 19400.00 0.00 0.04683 27000.00 19400.00 0.00 0.04687 26600.00 19600.00 0.00 0.04746 26800.00 19600.00 0.00 0.04812 27000.00 19600.00 0.00 0.04821 26600.00 19800.00 0.00 0.04876 26800.00 19800.00 0.00 0.04948 27000.00 19800.00 0.00 0.04962 26600.00 20000.00 0.00 0.05016 26800.00 20000.00 0.00 0.05095 27000.00 20000.00 0.00 0.05109 26600.00 20200.00 0.00 0.05150 26800.00 20200.00 0.00 0.05230 27000.00 20200.00 0.00 0.05240 26600.00 20400.00 0.00 0.05267 26800.00 20400.00 0.00 0.05349 27000.00 20400.00 0.00 0.05356 26600.00 20600.00 0.00 0.05403 26800.00 20600.00 0.00 0.05488 27000.00 20600.00 0.00 0.05492 26600.00 20800.00 0.00 0.05535 26800.00 20800.00 0.00 0.05621 26600.00 21000.00 0.00 0.05664 26800.00 21000.00 0.00 0.05749 26600.00 21200.00 0.00 0.05802 26800.00 21200.00 0.00 0.05884 26600.00 21400.00 0.00 0.05939 26800.00 21400.00 0.00 0.06018 26600.00 21600.00 0.00 0.06078 26800.00 21600.00 0.00 0.06153 26600.00 21800.00 0.00 0.06215 26800.00 21800.00 0.00 0.06284 26600.00 22000.00 0.00 0.06353 26800.00 22000.00 0.00 0.06416 26600.00 22200.00 0.00 0.06488 26800.00 22200.00 0.00 0.06554 26600.00 22400.00 0.00 0.06630 26800.00 22400.00 0.00 0.06702 26600.00 22600.00 0.00 0.06788 26800.00 22600.00 0.00 0.06864 26600.00 22800.00 0.00 0.06959 26800.00 22800.00 0.00 0.07035 26600.00 23000.00 0.00 0.07123 26800.00 23000.00 0.00 0.07198 26600.00 23200.00 0.00 0.07288 26800.00 23200.00 0.00 0.07359 26600.00 23400.00 0.00 0.07452 26800.00 23400.00 0.00 0.07521 26600.00 23600.00 0.00 0.07636 26800.00 23600.00 0.00 0.07700 26600.00 23800.00 0.00 0.07837 26800.00 23800.00 0.00 0.07892 26600.00 24000.00 0.00 0.08022 26800.00 24000.00 0.00 0.08072 26600.00 24200.00 0.00 0.08234 26800.00 24200.00 0.00 0.08288 26600.00 24400.00 0.00 0.08467 26800.00 24400.00 0.00 0.08519 26600.00 24600.00 0.00 0.08714 26800.00 24600.00 0.00 0.08759 26600.00 24800.00 0.00 0.08960 26800.00 24800.00 0.00 0.08999 26400.00 25000.00 0.00 0.09029 26600.00 25000.00 0.00 0.09231 26800.00 25000.00 0.00 0.09266 26400.00 25200.00 0.00 0.09344 26600.00 25200.00 0.00 0.09546 26800.00 25200.00 0.00 0.09568 26400.00 25400.00 0.00 0.09648 26600.00 25400.00 0.00 0.09842 26800.00 25400.00 0.00 0.09844 26400.00 25600.00 0.00 0.09981 26600.00 25600.00 0.00 0.10160 26400.00 25800.00 0.00 0.10314 26600.00 25800.00 0.00 0.10475 26400.00 26000.00 0.00 0.10636 26600.00 26000.00 0.00 0.10771 26400.00 26200.00 0.00 0.10930 26600.00 26200.00 0.00 0.11031 26400.00 26400.00 0.00 0.11167 26600.00 26400.00 0.00 0.11220 26400.00 26600.00 0.00 0.11277 26400.00 26600.00 5.00 0.11294 26400.00 26600.00 10.00 0.11310 26400.00 26600.00 15.00 0.11325 26400.00 26600.00 20.00 0.11337 26400.00 26600.00 25.00 0.11347 26400.00 26600.00 30.00 0.11354 26400.00 26600.00 35.00 0.11358 26400.00 26600.00 40.00 0.11358 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 26401.24 26629.94 36.85 0.11362 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/208.ctf NX, NY, NZ: 512 512 1 MODE : real