CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/205.mrc Output diagnostic file name Micrographs_0601/205.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/205.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2504.000 8902.000 Mean, RMS : 4511.792 219.1456 TITLE 1: emi2mrc: 205.emi Sun Jan 05 17:33:52 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 42.377, Y 528.916, Z -81.867, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01708 15000.00 10000.00 0.00 -0.01689 15200.00 10000.00 0.00 -0.01650 15400.00 10000.00 0.00 -0.01619 15600.00 10000.00 0.00 -0.01590 15800.00 10000.00 0.00 -0.01549 16000.00 10000.00 0.00 -0.01516 16200.00 10000.00 0.00 -0.01502 16400.00 10000.00 0.00 -0.01469 16600.00 10000.00 0.00 -0.01415 16800.00 10000.00 0.00 -0.01366 17000.00 10000.00 0.00 -0.01311 17200.00 10000.00 0.00 -0.01237 17400.00 10000.00 0.00 -0.01171 17600.00 10000.00 0.00 -0.01105 17800.00 10000.00 0.00 -0.01037 18000.00 10000.00 0.00 -0.00967 18200.00 10000.00 0.00 -0.00897 18400.00 10000.00 0.00 -0.00837 18600.00 10000.00 0.00 -0.00762 18800.00 10000.00 0.00 -0.00684 19000.00 10000.00 0.00 -0.00606 19200.00 10000.00 0.00 -0.00517 19400.00 10000.00 0.00 -0.00425 19600.00 10000.00 0.00 -0.00345 19800.00 10000.00 0.00 -0.00273 20000.00 10000.00 0.00 -0.00178 20200.00 10000.00 0.00 -0.00079 20400.00 10000.00 0.00 0.00020 20600.00 10000.00 0.00 0.00114 20800.00 10000.00 0.00 0.00193 21000.00 10000.00 0.00 0.00282 21200.00 10000.00 0.00 0.00371 21400.00 10000.00 0.00 0.00463 21600.00 10000.00 0.00 0.00568 21800.00 10000.00 0.00 0.00670 22000.00 10000.00 0.00 0.00756 22200.00 10000.00 0.00 0.00846 22400.00 10000.00 0.00 0.00961 22600.00 10000.00 0.00 0.01080 22800.00 10000.00 0.00 0.01199 23000.00 10000.00 0.00 0.01304 23200.00 10000.00 0.00 0.01392 23400.00 10000.00 0.00 0.01507 23600.00 10000.00 0.00 0.01642 23800.00 10000.00 0.00 0.01778 24000.00 10000.00 0.00 0.01918 24200.00 10000.00 0.00 0.02080 24400.00 10000.00 0.00 0.02265 24600.00 10000.00 0.00 0.02460 24800.00 10000.00 0.00 0.02669 25000.00 10000.00 0.00 0.02847 25200.00 10000.00 0.00 0.02958 25400.00 10000.00 0.00 0.03012 25600.00 10000.00 0.00 0.03038 25800.00 10000.00 0.00 0.03047 25400.00 10200.00 0.00 0.03061 25600.00 10200.00 0.00 0.03087 25800.00 10200.00 0.00 0.03093 25400.00 10400.00 0.00 0.03102 25600.00 10400.00 0.00 0.03130 25800.00 10400.00 0.00 0.03133 25400.00 10600.00 0.00 0.03137 25600.00 10600.00 0.00 0.03166 25600.00 10800.00 0.00 0.03179 25600.00 11000.00 0.00 0.03188 25400.00 11200.00 0.00 0.03198 25600.00 11200.00 0.00 0.03210 25400.00 11400.00 0.00 0.03223 25600.00 11400.00 0.00 0.03234 25400.00 11600.00 0.00 0.03247 25600.00 11600.00 0.00 0.03257 25400.00 11800.00 0.00 0.03280 25600.00 11800.00 0.00 0.03291 25400.00 12000.00 0.00 0.03340 25600.00 12000.00 0.00 0.03350 25400.00 12200.00 0.00 0.03408 25600.00 12200.00 0.00 0.03417 25400.00 12400.00 0.00 0.03464 25600.00 12400.00 0.00 0.03472 25400.00 12600.00 0.00 0.03519 25600.00 12600.00 0.00 0.03528 25400.00 12800.00 0.00 0.03581 25600.00 12800.00 0.00 0.03589 25400.00 13000.00 0.00 0.03632 25600.00 13000.00 0.00 0.03636 25400.00 13200.00 0.00 0.03680 25600.00 13200.00 0.00 0.03685 25400.00 13400.00 0.00 0.03738 25600.00 13400.00 0.00 0.03744 25400.00 13600.00 0.00 0.03788 25400.00 13800.00 0.00 0.03831 25200.00 14000.00 0.00 0.03844 25400.00 14000.00 0.00 0.03886 25200.00 14200.00 0.00 0.03907 25400.00 14200.00 0.00 0.03946 25200.00 14400.00 0.00 0.03965 25400.00 14400.00 0.00 0.03999 25200.00 14600.00 0.00 0.04027 25400.00 14600.00 0.00 0.04058 25200.00 14800.00 0.00 0.04095 25400.00 14800.00 0.00 0.04126 25600.00 14800.00 0.00 0.04132 25800.00 14800.00 0.00 0.04134 25200.00 15000.00 0.00 0.04165 25400.00 15000.00 0.00 0.04203 25600.00 15000.00 0.00 0.04217 25800.00 15000.00 0.00 0.04218 25200.00 15200.00 0.00 0.04237 25400.00 15200.00 0.00 0.04282 25600.00 15200.00 0.00 0.04298 25200.00 15400.00 0.00 0.04318 25400.00 15400.00 0.00 0.04365 25600.00 15400.00 0.00 0.04379 25200.00 15600.00 0.00 0.04409 25400.00 15600.00 0.00 0.04454 25600.00 15600.00 0.00 0.04466 25200.00 15800.00 0.00 0.04493 25400.00 15800.00 0.00 0.04539 25600.00 15800.00 0.00 0.04550 25200.00 16000.00 0.00 0.04576 25400.00 16000.00 0.00 0.04626 25600.00 16000.00 0.00 0.04639 25200.00 16200.00 0.00 0.04666 25400.00 16200.00 0.00 0.04723 25600.00 16200.00 0.00 0.04737 25200.00 16400.00 0.00 0.04773 25400.00 16400.00 0.00 0.04833 25600.00 16400.00 0.00 0.04846 25200.00 16600.00 0.00 0.04885 25400.00 16600.00 0.00 0.04940 25600.00 16600.00 0.00 0.04948 25200.00 16800.00 0.00 0.04991 25400.00 16800.00 0.00 0.05041 25600.00 16800.00 0.00 0.05047 25200.00 17000.00 0.00 0.05102 25400.00 17000.00 0.00 0.05149 25600.00 17000.00 0.00 0.05156 25200.00 17200.00 0.00 0.05213 25400.00 17200.00 0.00 0.05260 25600.00 17200.00 0.00 0.05267 25200.00 17400.00 0.00 0.05329 25400.00 17400.00 0.00 0.05378 25600.00 17400.00 0.00 0.05382 25200.00 17600.00 0.00 0.05455 25400.00 17600.00 0.00 0.05503 25200.00 17800.00 0.00 0.05584 25400.00 17800.00 0.00 0.05622 25200.00 18000.00 0.00 0.05695 25400.00 18000.00 0.00 0.05723 25200.00 18200.00 0.00 0.05805 25400.00 18200.00 0.00 0.05830 25200.00 18400.00 0.00 0.05921 25400.00 18400.00 0.00 0.05946 25200.00 18600.00 0.00 0.06034 25400.00 18600.00 0.00 0.06058 25200.00 18800.00 0.00 0.06161 25400.00 18800.00 0.00 0.06181 25000.00 19000.00 0.00 0.06182 25200.00 19000.00 0.00 0.06285 25400.00 19000.00 0.00 0.06303 25200.00 19200.00 0.00 0.06405 25400.00 19200.00 0.00 0.06430 25000.00 19400.00 0.00 0.06442 25200.00 19400.00 0.00 0.06557 25400.00 19400.00 0.00 0.06588 25000.00 19600.00 0.00 0.06616 25200.00 19600.00 0.00 0.06734 25400.00 19600.00 0.00 0.06767 25000.00 19800.00 0.00 0.06777 25200.00 19800.00 0.00 0.06895 25400.00 19800.00 0.00 0.06925 25200.00 20000.00 0.00 0.07038 25400.00 20000.00 0.00 0.07066 25200.00 20200.00 0.00 0.07177 25400.00 20200.00 0.00 0.07203 25000.00 20400.00 0.00 0.07205 25200.00 20400.00 0.00 0.07326 25400.00 20400.00 0.00 0.07350 25000.00 20600.00 0.00 0.07365 25200.00 20600.00 0.00 0.07488 25400.00 20600.00 0.00 0.07510 25000.00 20800.00 0.00 0.07523 25200.00 20800.00 0.00 0.07647 25400.00 20800.00 0.00 0.07666 25000.00 21000.00 0.00 0.07685 25200.00 21000.00 0.00 0.07807 25400.00 21000.00 0.00 0.07815 25000.00 21200.00 0.00 0.07847 25200.00 21200.00 0.00 0.07961 25000.00 21400.00 0.00 0.08012 25200.00 21400.00 0.00 0.08117 25000.00 21600.00 0.00 0.08159 25200.00 21600.00 0.00 0.08255 25000.00 21800.00 0.00 0.08305 25200.00 21800.00 0.00 0.08397 25000.00 22000.00 0.00 0.08467 25200.00 22000.00 0.00 0.08552 25000.00 22200.00 0.00 0.08625 25200.00 22200.00 0.00 0.08703 25000.00 22400.00 0.00 0.08809 25200.00 22400.00 0.00 0.08879 25000.00 22600.00 0.00 0.09019 25200.00 22600.00 0.00 0.09084 25000.00 22800.00 0.00 0.09245 25200.00 22800.00 0.00 0.09301 25000.00 23000.00 0.00 0.09463 25200.00 23000.00 0.00 0.09513 25000.00 23200.00 0.00 0.09701 25200.00 23200.00 0.00 0.09745 25000.00 23400.00 0.00 0.09951 25200.00 23400.00 0.00 0.09982 25000.00 23600.00 0.00 0.10184 25200.00 23600.00 0.00 0.10198 24800.00 23800.00 0.00 0.10228 25000.00 23800.00 0.00 0.10434 24800.00 24000.00 0.00 0.10491 25000.00 24000.00 0.00 0.10685 24800.00 24200.00 0.00 0.10775 25000.00 24200.00 0.00 0.10953 24800.00 24400.00 0.00 0.11043 25000.00 24400.00 0.00 0.11200 24800.00 24600.00 0.00 0.11320 25000.00 24600.00 0.00 0.11445 24800.00 24800.00 0.00 0.11592 25000.00 24800.00 0.00 0.11655 24800.00 25000.00 0.00 0.11699 24800.00 25000.00 5.00 0.11715 24800.00 25000.00 10.00 0.11730 24800.00 25000.00 15.00 0.11744 24800.00 25000.00 20.00 0.11756 24800.00 25000.00 25.00 0.11766 24800.00 25000.00 30.00 0.11773 24800.00 25000.00 35.00 0.11777 24800.00 25000.00 40.00 0.11777 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24815.70 25060.59 33.57 0.11791 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/205.ctf NX, NY, NZ: 512 512 1 MODE : real