CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/204.mrc Output diagnostic file name Micrographs_0601/204.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/204.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2334.000 8192.000 Mean, RMS : 4508.699 223.9845 TITLE 1: emi2mrc: 204.emi Sun Jan 05 17:33:05 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 45.070, Y 528.582, Z -81.867, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01261 10800.00 10000.00 0.00 -0.01257 11200.00 10000.00 0.00 -0.01257 11400.00 10000.00 0.00 -0.01237 11600.00 10000.00 0.00 -0.01229 12600.00 10000.00 0.00 -0.01225 12800.00 10000.00 0.00 -0.01218 13000.00 10000.00 0.00 -0.01214 13200.00 10000.00 0.00 -0.01199 13400.00 10000.00 0.00 -0.01185 13600.00 10000.00 0.00 -0.01170 13800.00 10000.00 0.00 -0.01151 14000.00 10000.00 0.00 -0.01132 14200.00 10000.00 0.00 -0.01117 14400.00 10000.00 0.00 -0.01088 14600.00 10000.00 0.00 -0.01059 14800.00 10000.00 0.00 -0.01034 15000.00 10000.00 0.00 -0.00997 15200.00 10000.00 0.00 -0.00955 15400.00 10000.00 0.00 -0.00906 15600.00 10000.00 0.00 -0.00868 15800.00 10000.00 0.00 -0.00835 16000.00 10000.00 0.00 -0.00788 16200.00 10000.00 0.00 -0.00740 16400.00 10000.00 0.00 -0.00693 16600.00 10000.00 0.00 -0.00653 16800.00 10000.00 0.00 -0.00618 17000.00 10000.00 0.00 -0.00579 17200.00 10000.00 0.00 -0.00529 17400.00 10000.00 0.00 -0.00465 17600.00 10000.00 0.00 -0.00383 17800.00 10000.00 0.00 -0.00290 18000.00 10000.00 0.00 -0.00216 18200.00 10000.00 0.00 -0.00143 18400.00 10000.00 0.00 -0.00055 18600.00 10000.00 0.00 0.00021 18800.00 10000.00 0.00 0.00100 19000.00 10000.00 0.00 0.00189 19200.00 10000.00 0.00 0.00284 19400.00 10000.00 0.00 0.00384 19600.00 10000.00 0.00 0.00480 19800.00 10000.00 0.00 0.00576 20000.00 10000.00 0.00 0.00666 20200.00 10000.00 0.00 0.00756 20400.00 10000.00 0.00 0.00839 20600.00 10000.00 0.00 0.00926 20800.00 10000.00 0.00 0.01036 21000.00 10000.00 0.00 0.01145 21200.00 10000.00 0.00 0.01257 21400.00 10000.00 0.00 0.01383 21600.00 10000.00 0.00 0.01501 21800.00 10000.00 0.00 0.01608 22000.00 10000.00 0.00 0.01721 22200.00 10000.00 0.00 0.01846 22400.00 10000.00 0.00 0.01977 22600.00 10000.00 0.00 0.02111 22800.00 10000.00 0.00 0.02260 23000.00 10000.00 0.00 0.02425 23200.00 10000.00 0.00 0.02594 23400.00 10000.00 0.00 0.02780 23600.00 10000.00 0.00 0.02975 23800.00 10000.00 0.00 0.03175 24000.00 10000.00 0.00 0.03377 24200.00 10000.00 0.00 0.03549 24400.00 10000.00 0.00 0.03660 24600.00 10000.00 0.00 0.03708 24800.00 10000.00 0.00 0.03727 25000.00 10000.00 0.00 0.03738 25200.00 10000.00 0.00 0.03738 24800.00 10200.00 0.00 0.03755 25000.00 10200.00 0.00 0.03767 24800.00 10400.00 0.00 0.03793 25000.00 10400.00 0.00 0.03803 24600.00 10600.00 0.00 0.03815 24800.00 10600.00 0.00 0.03843 25000.00 10600.00 0.00 0.03851 24600.00 10800.00 0.00 0.03866 24800.00 10800.00 0.00 0.03894 25000.00 10800.00 0.00 0.03902 24600.00 11000.00 0.00 0.03926 24800.00 11000.00 0.00 0.03955 25000.00 11000.00 0.00 0.03962 24600.00 11200.00 0.00 0.03990 24800.00 11200.00 0.00 0.04016 25000.00 11200.00 0.00 0.04019 24600.00 11400.00 0.00 0.04051 24800.00 11400.00 0.00 0.04076 25000.00 11400.00 0.00 0.04080 24600.00 11600.00 0.00 0.04121 24800.00 11600.00 0.00 0.04150 25000.00 11600.00 0.00 0.04157 24600.00 11800.00 0.00 0.04211 24800.00 11800.00 0.00 0.04241 25000.00 11800.00 0.00 0.04247 24600.00 12000.00 0.00 0.04296 24800.00 12000.00 0.00 0.04323 25000.00 12000.00 0.00 0.04326 24600.00 12200.00 0.00 0.04374 24800.00 12200.00 0.00 0.04401 25000.00 12200.00 0.00 0.04403 24600.00 12400.00 0.00 0.04461 24800.00 12400.00 0.00 0.04484 24600.00 12600.00 0.00 0.04526 24800.00 12600.00 0.00 0.04543 24600.00 12800.00 0.00 0.04578 24800.00 12800.00 0.00 0.04595 24600.00 13000.00 0.00 0.04632 24800.00 13000.00 0.00 0.04646 24600.00 13200.00 0.00 0.04678 24800.00 13200.00 0.00 0.04686 24600.00 13400.00 0.00 0.04726 24800.00 13400.00 0.00 0.04731 24600.00 13600.00 0.00 0.04782 24800.00 13600.00 0.00 0.04786 24400.00 13800.00 0.00 0.04794 24600.00 13800.00 0.00 0.04847 24800.00 13800.00 0.00 0.04848 24400.00 14000.00 0.00 0.04863 24600.00 14000.00 0.00 0.04914 24400.00 14200.00 0.00 0.04927 24600.00 14200.00 0.00 0.04972 24400.00 14400.00 0.00 0.04990 24600.00 14400.00 0.00 0.05031 24400.00 14600.00 0.00 0.05060 24600.00 14600.00 0.00 0.05098 24400.00 14800.00 0.00 0.05133 24600.00 14800.00 0.00 0.05172 24400.00 15000.00 0.00 0.05211 24600.00 15000.00 0.00 0.05251 24400.00 15200.00 0.00 0.05287 24600.00 15200.00 0.00 0.05327 24400.00 15400.00 0.00 0.05378 24600.00 15400.00 0.00 0.05423 24400.00 15600.00 0.00 0.05476 24600.00 15600.00 0.00 0.05522 24400.00 15800.00 0.00 0.05567 24600.00 15800.00 0.00 0.05611 24400.00 16000.00 0.00 0.05666 24600.00 16000.00 0.00 0.05712 24400.00 16200.00 0.00 0.05768 24600.00 16200.00 0.00 0.05816 24800.00 16200.00 0.00 0.05819 24400.00 16400.00 0.00 0.05886 24600.00 16400.00 0.00 0.05934 24800.00 16400.00 0.00 0.05938 24400.00 16600.00 0.00 0.06001 24600.00 16600.00 0.00 0.06048 24800.00 16600.00 0.00 0.06052 24400.00 16800.00 0.00 0.06109 24600.00 16800.00 0.00 0.06160 24800.00 16800.00 0.00 0.06164 24400.00 17000.00 0.00 0.06234 24600.00 17000.00 0.00 0.06286 24400.00 17200.00 0.00 0.06366 24600.00 17200.00 0.00 0.06413 24400.00 17400.00 0.00 0.06507 24600.00 17400.00 0.00 0.06547 24400.00 17600.00 0.00 0.06646 24600.00 17600.00 0.00 0.06682 24400.00 17800.00 0.00 0.06783 24600.00 17800.00 0.00 0.06818 24400.00 18000.00 0.00 0.06928 24600.00 18000.00 0.00 0.06961 24400.00 18200.00 0.00 0.07083 24600.00 18200.00 0.00 0.07112 24400.00 18400.00 0.00 0.07227 24600.00 18400.00 0.00 0.07254 24400.00 18600.00 0.00 0.07371 24600.00 18600.00 0.00 0.07395 24400.00 18800.00 0.00 0.07523 24600.00 18800.00 0.00 0.07540 24400.00 19000.00 0.00 0.07671 24600.00 19000.00 0.00 0.07683 24200.00 19200.00 0.00 0.07698 24400.00 19200.00 0.00 0.07830 24600.00 19200.00 0.00 0.07837 24200.00 19400.00 0.00 0.07852 24400.00 19400.00 0.00 0.07980 24600.00 19400.00 0.00 0.07981 24200.00 19600.00 0.00 0.08011 24400.00 19600.00 0.00 0.08133 24200.00 19800.00 0.00 0.08170 24400.00 19800.00 0.00 0.08282 24200.00 20000.00 0.00 0.08317 24400.00 20000.00 0.00 0.08420 24200.00 20200.00 0.00 0.08467 24400.00 20200.00 0.00 0.08566 24200.00 20400.00 0.00 0.08630 24400.00 20400.00 0.00 0.08725 24200.00 20600.00 0.00 0.08812 24400.00 20600.00 0.00 0.08901 24200.00 20800.00 0.00 0.08989 24400.00 20800.00 0.00 0.09076 24200.00 21000.00 0.00 0.09173 24400.00 21000.00 0.00 0.09259 24200.00 21200.00 0.00 0.09352 24400.00 21200.00 0.00 0.09435 24200.00 21400.00 0.00 0.09537 24400.00 21400.00 0.00 0.09612 24200.00 21600.00 0.00 0.09728 24400.00 21600.00 0.00 0.09794 24200.00 21800.00 0.00 0.09919 24400.00 21800.00 0.00 0.09982 24200.00 22000.00 0.00 0.10138 24400.00 22000.00 0.00 0.10198 24200.00 22200.00 0.00 0.10368 24400.00 22200.00 0.00 0.10422 24200.00 22400.00 0.00 0.10616 24400.00 22400.00 0.00 0.10663 24200.00 22600.00 0.00 0.10868 24400.00 22600.00 0.00 0.10910 24000.00 22800.00 0.00 0.10915 24200.00 22800.00 0.00 0.11132 24400.00 22800.00 0.00 0.11162 24000.00 23000.00 0.00 0.11177 24200.00 23000.00 0.00 0.11389 24400.00 23000.00 0.00 0.11400 24000.00 23200.00 0.00 0.11446 24200.00 23200.00 0.00 0.11650 24000.00 23400.00 0.00 0.11738 24200.00 23400.00 0.00 0.11932 24000.00 23600.00 0.00 0.12032 24200.00 23600.00 0.00 0.12204 24000.00 23800.00 0.00 0.12333 24200.00 23800.00 0.00 0.12461 24000.00 24000.00 0.00 0.12561 24200.00 24000.00 0.00 0.12642 24000.00 24200.00 0.00 0.12698 24200.00 24200.00 0.00 0.12713 24000.00 24200.00 10.00 0.12725 24000.00 24200.00 15.00 0.12736 24000.00 24200.00 20.00 0.12746 24000.00 24200.00 25.00 0.12753 24000.00 24200.00 30.00 0.12758 24000.00 24200.00 35.00 0.12760 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24000.00 24200.00 35.00 0.12760 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/204.ctf NX, NY, NZ: 512 512 1 MODE : real