CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/203.mrc Output diagnostic file name Micrographs_0601/203.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/203.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2364.000 8290.000 Mean, RMS : 4510.247 218.4283 TITLE 1: emi2mrc: 203.emi Sun Jan 05 17:31:58 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 47.831, Y 528.148, Z -81.867, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01158 10200.00 10000.00 0.00 -0.01156 10400.00 10000.00 0.00 -0.01154 11400.00 10000.00 0.00 -0.01144 11600.00 10000.00 0.00 -0.01136 11800.00 10000.00 0.00 -0.01130 12800.00 10000.00 0.00 -0.01128 13000.00 10000.00 0.00 -0.01097 13200.00 10000.00 0.00 -0.01066 13400.00 10000.00 0.00 -0.01049 13600.00 10000.00 0.00 -0.01011 13800.00 10000.00 0.00 -0.00968 14000.00 10000.00 0.00 -0.00916 14200.00 10000.00 0.00 -0.00860 14400.00 10000.00 0.00 -0.00811 14600.00 10000.00 0.00 -0.00746 14800.00 10000.00 0.00 -0.00679 15000.00 10000.00 0.00 -0.00612 15200.00 10000.00 0.00 -0.00540 15400.00 10000.00 0.00 -0.00463 15600.00 10000.00 0.00 -0.00388 15800.00 10000.00 0.00 -0.00329 16000.00 10000.00 0.00 -0.00262 16200.00 10000.00 0.00 -0.00199 16400.00 10000.00 0.00 -0.00146 16600.00 10000.00 0.00 -0.00090 16800.00 10000.00 0.00 -0.00032 17000.00 10000.00 0.00 0.00042 17200.00 10000.00 0.00 0.00132 17400.00 10000.00 0.00 0.00220 17600.00 10000.00 0.00 0.00299 17800.00 10000.00 0.00 0.00390 18000.00 10000.00 0.00 0.00486 18200.00 10000.00 0.00 0.00567 18400.00 10000.00 0.00 0.00655 18600.00 10000.00 0.00 0.00761 18800.00 10000.00 0.00 0.00866 19000.00 10000.00 0.00 0.00960 19200.00 10000.00 0.00 0.01055 19400.00 10000.00 0.00 0.01143 19600.00 10000.00 0.00 0.01236 19800.00 10000.00 0.00 0.01333 20000.00 10000.00 0.00 0.01426 20200.00 10000.00 0.00 0.01542 20400.00 10000.00 0.00 0.01655 20600.00 10000.00 0.00 0.01756 20800.00 10000.00 0.00 0.01871 21000.00 10000.00 0.00 0.01994 21200.00 10000.00 0.00 0.02133 21400.00 10000.00 0.00 0.02269 21600.00 10000.00 0.00 0.02396 21800.00 10000.00 0.00 0.02516 22000.00 10000.00 0.00 0.02642 22200.00 10000.00 0.00 0.02782 22400.00 10000.00 0.00 0.02921 22600.00 10000.00 0.00 0.03071 22800.00 10000.00 0.00 0.03249 23000.00 10000.00 0.00 0.03444 23200.00 10000.00 0.00 0.03644 23400.00 10000.00 0.00 0.03832 23600.00 10000.00 0.00 0.03971 23800.00 10000.00 0.00 0.04034 24000.00 10000.00 0.00 0.04035 23800.00 10200.00 0.00 0.04062 23800.00 10400.00 0.00 0.04083 23800.00 10600.00 0.00 0.04119 23800.00 10800.00 0.00 0.04165 24000.00 10800.00 0.00 0.04167 23800.00 11000.00 0.00 0.04216 24000.00 11000.00 0.00 0.04221 23800.00 11200.00 0.00 0.04263 24000.00 11200.00 0.00 0.04265 23800.00 11400.00 0.00 0.04303 24000.00 11400.00 0.00 0.04304 23800.00 11600.00 0.00 0.04357 24000.00 11600.00 0.00 0.04360 24200.00 11600.00 0.00 0.04363 23600.00 11800.00 0.00 0.04367 23800.00 11800.00 0.00 0.04421 24000.00 11800.00 0.00 0.04423 24200.00 11800.00 0.00 0.04425 23800.00 12000.00 0.00 0.04473 24000.00 12000.00 0.00 0.04473 24200.00 12000.00 0.00 0.04474 23600.00 12200.00 0.00 0.04484 23800.00 12200.00 0.00 0.04531 24000.00 12200.00 0.00 0.04532 24200.00 12200.00 0.00 0.04536 23600.00 12400.00 0.00 0.04558 23800.00 12400.00 0.00 0.04597 24000.00 12400.00 0.00 0.04599 24200.00 12400.00 0.00 0.04601 23600.00 12600.00 0.00 0.04631 23800.00 12600.00 0.00 0.04666 24000.00 12600.00 0.00 0.04671 24200.00 12600.00 0.00 0.04672 23600.00 12800.00 0.00 0.04711 23800.00 12800.00 0.00 0.04749 24000.00 12800.00 0.00 0.04759 23600.00 13000.00 0.00 0.04789 23800.00 13000.00 0.00 0.04834 24000.00 13000.00 0.00 0.04842 23600.00 13200.00 0.00 0.04875 23800.00 13200.00 0.00 0.04919 24000.00 13200.00 0.00 0.04920 23600.00 13400.00 0.00 0.04941 23800.00 13400.00 0.00 0.04984 23600.00 13600.00 0.00 0.05009 23800.00 13600.00 0.00 0.05055 24000.00 13600.00 0.00 0.05058 24200.00 13600.00 0.00 0.05059 23600.00 13800.00 0.00 0.05090 23800.00 13800.00 0.00 0.05137 24000.00 13800.00 0.00 0.05138 23600.00 14000.00 0.00 0.05156 23800.00 14000.00 0.00 0.05196 23600.00 14200.00 0.00 0.05231 23800.00 14200.00 0.00 0.05267 24000.00 14200.00 0.00 0.05270 24200.00 14200.00 0.00 0.05276 23600.00 14400.00 0.00 0.05312 23800.00 14400.00 0.00 0.05351 24000.00 14400.00 0.00 0.05362 24200.00 14400.00 0.00 0.05369 23600.00 14600.00 0.00 0.05410 23800.00 14600.00 0.00 0.05453 24000.00 14600.00 0.00 0.05465 23600.00 14800.00 0.00 0.05519 23800.00 14800.00 0.00 0.05561 24000.00 14800.00 0.00 0.05568 23600.00 15000.00 0.00 0.05617 23800.00 15000.00 0.00 0.05657 24000.00 15000.00 0.00 0.05660 23600.00 15200.00 0.00 0.05722 23800.00 15200.00 0.00 0.05762 24000.00 15200.00 0.00 0.05764 23600.00 15400.00 0.00 0.05834 23800.00 15400.00 0.00 0.05873 24000.00 15400.00 0.00 0.05877 24200.00 15400.00 0.00 0.05877 23600.00 15600.00 0.00 0.05938 23800.00 15600.00 0.00 0.05975 24000.00 15600.00 0.00 0.05981 24200.00 15600.00 0.00 0.05982 23600.00 15800.00 0.00 0.06038 23800.00 15800.00 0.00 0.06075 24000.00 15800.00 0.00 0.06085 24200.00 15800.00 0.00 0.06087 23600.00 16000.00 0.00 0.06144 23800.00 16000.00 0.00 0.06184 24000.00 16000.00 0.00 0.06197 23600.00 16200.00 0.00 0.06253 23800.00 16200.00 0.00 0.06295 24000.00 16200.00 0.00 0.06306 23600.00 16400.00 0.00 0.06380 23800.00 16400.00 0.00 0.06420 24000.00 16400.00 0.00 0.06427 23600.00 16600.00 0.00 0.06507 23800.00 16600.00 0.00 0.06544 24000.00 16600.00 0.00 0.06544 23600.00 16800.00 0.00 0.06632 23800.00 16800.00 0.00 0.06667 23600.00 17000.00 0.00 0.06754 23800.00 17000.00 0.00 0.06784 23600.00 17200.00 0.00 0.06864 23800.00 17200.00 0.00 0.06892 23600.00 17400.00 0.00 0.06988 23800.00 17400.00 0.00 0.07017 23600.00 17600.00 0.00 0.07113 23800.00 17600.00 0.00 0.07145 23600.00 17800.00 0.00 0.07261 23800.00 17800.00 0.00 0.07295 23600.00 18000.00 0.00 0.07426 23800.00 18000.00 0.00 0.07462 23600.00 18200.00 0.00 0.07594 23800.00 18200.00 0.00 0.07624 23600.00 18400.00 0.00 0.07758 23800.00 18400.00 0.00 0.07782 23400.00 18600.00 0.00 0.07786 23600.00 18600.00 0.00 0.07923 23800.00 18600.00 0.00 0.07943 23400.00 18800.00 0.00 0.07961 23600.00 18800.00 0.00 0.08094 23800.00 18800.00 0.00 0.08111 23400.00 19000.00 0.00 0.08124 23600.00 19000.00 0.00 0.08255 23800.00 19000.00 0.00 0.08269 23400.00 19200.00 0.00 0.08300 23600.00 19200.00 0.00 0.08427 23800.00 19200.00 0.00 0.08435 23400.00 19400.00 0.00 0.08462 23600.00 19400.00 0.00 0.08581 23400.00 19600.00 0.00 0.08610 23600.00 19600.00 0.00 0.08724 23400.00 19800.00 0.00 0.08766 23600.00 19800.00 0.00 0.08881 23400.00 20000.00 0.00 0.08934 23600.00 20000.00 0.00 0.09052 23400.00 20200.00 0.00 0.09103 23600.00 20200.00 0.00 0.09220 23400.00 20400.00 0.00 0.09258 23600.00 20400.00 0.00 0.09366 23400.00 20600.00 0.00 0.09426 23600.00 20600.00 0.00 0.09523 23400.00 20800.00 0.00 0.09602 23600.00 20800.00 0.00 0.09690 23400.00 21000.00 0.00 0.09785 23600.00 21000.00 0.00 0.09865 23400.00 21200.00 0.00 0.09963 23600.00 21200.00 0.00 0.10032 23400.00 21400.00 0.00 0.10146 23600.00 21400.00 0.00 0.10203 23400.00 21600.00 0.00 0.10325 23600.00 21600.00 0.00 0.10370 23400.00 21800.00 0.00 0.10502 23600.00 21800.00 0.00 0.10537 23400.00 22000.00 0.00 0.10704 23600.00 22000.00 0.00 0.10728 23400.00 22200.00 0.00 0.10903 23600.00 22200.00 0.00 0.10908 23400.00 22400.00 0.00 0.11118 23400.00 22600.00 0.00 0.11308 23400.00 22800.00 0.00 0.11485 23400.00 23000.00 0.00 0.11629 23400.00 23200.00 0.00 0.11745 23200.00 23400.00 0.00 0.11763 23400.00 23400.00 0.00 0.11838 23200.00 23600.00 20.00 0.11840 23200.00 23600.00 25.00 0.11850 23200.00 23600.00 30.00 0.11854 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23289.89 23505.96 29.26 0.11888 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/203.ctf NX, NY, NZ: 512 512 1 MODE : real