CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/201.mrc Output diagnostic file name Micrographs_0601/201.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/201.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2382.000 8348.000 Mean, RMS : 4515.266 222.7109 TITLE 1: emi2mrc: 201.emi Sun Jan 05 17:31:10 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X 50.340, Y 527.764, Z -81.867, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01255 10200.00 10000.00 0.00 -0.01249 10400.00 10000.00 0.00 -0.01235 10600.00 10000.00 0.00 -0.01228 10800.00 10000.00 0.00 -0.01226 11000.00 10000.00 0.00 -0.01223 11200.00 10000.00 0.00 -0.01203 11400.00 10000.00 0.00 -0.01195 12400.00 10000.00 0.00 -0.01186 12600.00 10000.00 0.00 -0.01167 12800.00 10000.00 0.00 -0.01153 13000.00 10000.00 0.00 -0.01137 13200.00 10000.00 0.00 -0.01105 13400.00 10000.00 0.00 -0.01060 13600.00 10000.00 0.00 -0.01004 13800.00 10000.00 0.00 -0.00956 14000.00 10000.00 0.00 -0.00922 14200.00 10000.00 0.00 -0.00883 14400.00 10000.00 0.00 -0.00842 14600.00 10000.00 0.00 -0.00807 14800.00 10000.00 0.00 -0.00774 15000.00 10000.00 0.00 -0.00724 15200.00 10000.00 0.00 -0.00660 15400.00 10000.00 0.00 -0.00599 15600.00 10000.00 0.00 -0.00535 15800.00 10000.00 0.00 -0.00465 16000.00 10000.00 0.00 -0.00393 16200.00 10000.00 0.00 -0.00317 16400.00 10000.00 0.00 -0.00234 16600.00 10000.00 0.00 -0.00149 16800.00 10000.00 0.00 -0.00060 17000.00 10000.00 0.00 0.00029 17200.00 10000.00 0.00 0.00125 17400.00 10000.00 0.00 0.00236 17600.00 10000.00 0.00 0.00344 17800.00 10000.00 0.00 0.00434 18000.00 10000.00 0.00 0.00516 18200.00 10000.00 0.00 0.00614 18400.00 10000.00 0.00 0.00721 18600.00 10000.00 0.00 0.00818 18800.00 10000.00 0.00 0.00912 19000.00 10000.00 0.00 0.01001 19200.00 10000.00 0.00 0.01088 19400.00 10000.00 0.00 0.01168 19600.00 10000.00 0.00 0.01247 19800.00 10000.00 0.00 0.01356 20000.00 10000.00 0.00 0.01479 20200.00 10000.00 0.00 0.01591 20400.00 10000.00 0.00 0.01706 20600.00 10000.00 0.00 0.01839 20800.00 10000.00 0.00 0.01975 21000.00 10000.00 0.00 0.02108 21200.00 10000.00 0.00 0.02228 21400.00 10000.00 0.00 0.02357 21600.00 10000.00 0.00 0.02511 21800.00 10000.00 0.00 0.02663 22000.00 10000.00 0.00 0.02829 22200.00 10000.00 0.00 0.03017 22400.00 10000.00 0.00 0.03235 22600.00 10000.00 0.00 0.03476 22800.00 10000.00 0.00 0.03684 23000.00 10000.00 0.00 0.03810 23200.00 10000.00 0.00 0.03849 23400.00 10000.00 0.00 0.03862 23600.00 10000.00 0.00 0.03881 23800.00 10000.00 0.00 0.03893 23400.00 10200.00 0.00 0.03898 23600.00 10200.00 0.00 0.03928 23800.00 10200.00 0.00 0.03942 23400.00 10400.00 0.00 0.03963 23600.00 10400.00 0.00 0.03993 23800.00 10400.00 0.00 0.03999 23400.00 10600.00 0.00 0.04012 23600.00 10600.00 0.00 0.04038 23800.00 10600.00 0.00 0.04039 23400.00 10800.00 0.00 0.04060 23600.00 10800.00 0.00 0.04085 23800.00 10800.00 0.00 0.04088 23400.00 11000.00 0.00 0.04124 23600.00 11000.00 0.00 0.04150 23800.00 11000.00 0.00 0.04153 23400.00 11200.00 0.00 0.04180 23600.00 11200.00 0.00 0.04202 23800.00 11200.00 0.00 0.04203 23200.00 11400.00 0.00 0.04206 23400.00 11400.00 0.00 0.04244 23600.00 11400.00 0.00 0.04265 23800.00 11400.00 0.00 0.04269 23200.00 11600.00 0.00 0.04271 23400.00 11600.00 0.00 0.04309 23600.00 11600.00 0.00 0.04332 23800.00 11600.00 0.00 0.04339 23200.00 11800.00 0.00 0.04345 23400.00 11800.00 0.00 0.04383 23600.00 11800.00 0.00 0.04405 23800.00 11800.00 0.00 0.04411 23200.00 12000.00 0.00 0.04426 23400.00 12000.00 0.00 0.04462 23600.00 12000.00 0.00 0.04484 23800.00 12000.00 0.00 0.04489 23200.00 12200.00 0.00 0.04500 23400.00 12200.00 0.00 0.04536 23600.00 12200.00 0.00 0.04558 23800.00 12200.00 0.00 0.04564 23200.00 12400.00 0.00 0.04580 23400.00 12400.00 0.00 0.04618 23600.00 12400.00 0.00 0.04644 23800.00 12400.00 0.00 0.04650 23200.00 12600.00 0.00 0.04664 23400.00 12600.00 0.00 0.04705 23600.00 12600.00 0.00 0.04731 23800.00 12600.00 0.00 0.04733 23200.00 12800.00 0.00 0.04746 23400.00 12800.00 0.00 0.04787 23600.00 12800.00 0.00 0.04810 23200.00 13000.00 0.00 0.04823 23400.00 13000.00 0.00 0.04862 23600.00 13000.00 0.00 0.04881 23200.00 13200.00 0.00 0.04902 23400.00 13200.00 0.00 0.04939 23600.00 13200.00 0.00 0.04955 23200.00 13400.00 0.00 0.04967 23400.00 13400.00 0.00 0.05000 23600.00 13400.00 0.00 0.05014 23200.00 13600.00 0.00 0.05020 23400.00 13600.00 0.00 0.05054 23600.00 13600.00 0.00 0.05068 23200.00 13800.00 0.00 0.05095 23400.00 13800.00 0.00 0.05129 23600.00 13800.00 0.00 0.05140 23200.00 14000.00 0.00 0.05191 23400.00 14000.00 0.00 0.05223 23600.00 14000.00 0.00 0.05230 23200.00 14200.00 0.00 0.05291 23400.00 14200.00 0.00 0.05319 23600.00 14200.00 0.00 0.05325 23200.00 14400.00 0.00 0.05381 23400.00 14400.00 0.00 0.05409 23600.00 14400.00 0.00 0.05416 23000.00 14600.00 0.00 0.05426 23200.00 14600.00 0.00 0.05490 23400.00 14600.00 0.00 0.05518 23600.00 14600.00 0.00 0.05528 23000.00 14800.00 0.00 0.05537 23200.00 14800.00 0.00 0.05601 23400.00 14800.00 0.00 0.05630 23600.00 14800.00 0.00 0.05643 23000.00 15000.00 0.00 0.05653 23200.00 15000.00 0.00 0.05719 23400.00 15000.00 0.00 0.05750 23600.00 15000.00 0.00 0.05764 23000.00 15200.00 0.00 0.05782 23200.00 15200.00 0.00 0.05848 23400.00 15200.00 0.00 0.05880 23600.00 15200.00 0.00 0.05890 23000.00 15400.00 0.00 0.05910 23200.00 15400.00 0.00 0.05974 23400.00 15400.00 0.00 0.06001 23600.00 15400.00 0.00 0.06006 23000.00 15600.00 0.00 0.06041 23200.00 15600.00 0.00 0.06102 23400.00 15600.00 0.00 0.06125 23000.00 15800.00 0.00 0.06165 23200.00 15800.00 0.00 0.06220 23400.00 15800.00 0.00 0.06237 23000.00 16000.00 0.00 0.06291 23200.00 16000.00 0.00 0.06340 23400.00 16000.00 0.00 0.06355 23000.00 16200.00 0.00 0.06420 23200.00 16200.00 0.00 0.06469 23400.00 16200.00 0.00 0.06483 23000.00 16400.00 0.00 0.06554 23200.00 16400.00 0.00 0.06600 23400.00 16400.00 0.00 0.06610 23000.00 16600.00 0.00 0.06688 23200.00 16600.00 0.00 0.06727 23400.00 16600.00 0.00 0.06734 23000.00 16800.00 0.00 0.06820 23200.00 16800.00 0.00 0.06856 23400.00 16800.00 0.00 0.06866 23000.00 17000.00 0.00 0.06952 23200.00 17000.00 0.00 0.06988 23400.00 17000.00 0.00 0.07003 23000.00 17200.00 0.00 0.07090 23200.00 17200.00 0.00 0.07130 23400.00 17200.00 0.00 0.07147 23000.00 17400.00 0.00 0.07253 23200.00 17400.00 0.00 0.07296 23400.00 17400.00 0.00 0.07310 23000.00 17600.00 0.00 0.07425 23200.00 17600.00 0.00 0.07464 23400.00 17600.00 0.00 0.07469 23000.00 17800.00 0.00 0.07596 23200.00 17800.00 0.00 0.07627 23400.00 17800.00 0.00 0.07629 23000.00 18000.00 0.00 0.07752 23200.00 18000.00 0.00 0.07785 23400.00 18000.00 0.00 0.07790 23000.00 18200.00 0.00 0.07904 23200.00 18200.00 0.00 0.07940 23400.00 18200.00 0.00 0.07949 23000.00 18400.00 0.00 0.08059 23200.00 18400.00 0.00 0.08101 23400.00 18400.00 0.00 0.08110 23000.00 18600.00 0.00 0.08223 23200.00 18600.00 0.00 0.08268 23400.00 18600.00 0.00 0.08276 23000.00 18800.00 0.00 0.08397 23200.00 18800.00 0.00 0.08439 23400.00 18800.00 0.00 0.08439 23000.00 19000.00 0.00 0.08579 23200.00 19000.00 0.00 0.08613 23000.00 19200.00 0.00 0.08764 23200.00 19200.00 0.00 0.08792 23000.00 19400.00 0.00 0.08936 23200.00 19400.00 0.00 0.08954 22800.00 19600.00 0.00 0.08972 23000.00 19600.00 0.00 0.09114 23200.00 19600.00 0.00 0.09123 22800.00 19800.00 0.00 0.09144 23000.00 19800.00 0.00 0.09279 23200.00 19800.00 0.00 0.09282 22800.00 20000.00 0.00 0.09321 23000.00 20000.00 0.00 0.09454 23200.00 20000.00 0.00 0.09455 22800.00 20200.00 0.00 0.09526 23000.00 20200.00 0.00 0.09660 22800.00 20400.00 0.00 0.09742 23000.00 20400.00 0.00 0.09870 22800.00 20600.00 0.00 0.09969 23000.00 20600.00 0.00 0.10090 22800.00 20800.00 0.00 0.10181 23000.00 20800.00 0.00 0.10294 22800.00 21000.00 0.00 0.10409 23000.00 21000.00 0.00 0.10513 22800.00 21200.00 0.00 0.10624 23000.00 21200.00 0.00 0.10717 22800.00 21400.00 0.00 0.10830 23000.00 21400.00 0.00 0.10922 22800.00 21600.00 0.00 0.11070 23000.00 21600.00 0.00 0.11163 22800.00 21800.00 0.00 0.11321 23000.00 21800.00 0.00 0.11402 22800.00 22000.00 0.00 0.11585 23000.00 22000.00 0.00 0.11642 22800.00 22200.00 0.00 0.11834 23000.00 22200.00 0.00 0.11858 22600.00 22400.00 0.00 0.11873 22800.00 22400.00 0.00 0.12072 22600.00 22600.00 0.00 0.12091 22800.00 22600.00 0.00 0.12265 22600.00 22800.00 0.00 0.12279 22800.00 22800.00 0.00 0.12426 22800.00 23000.00 0.00 0.12435 22800.00 23000.00 5.00 0.12448 22800.00 23000.00 10.00 0.12458 22800.00 23000.00 15.00 0.12465 22800.00 23000.00 20.00 0.12470 22800.00 23000.00 25.00 0.12471 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22738.69 22955.14 28.52 0.12498 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/201.ctf NX, NY, NZ: 512 512 1 MODE : real