CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/197.mrc Output diagnostic file name Micrographs_0601/197.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/197.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2356.000 8290.000 Mean, RMS : 4489.812 218.1831 TITLE 1: emi2mrc: 197.emi Sun Jan 05 17:25:30 2014 TITLE 2: kV 300, Mag 78000x, Def 24120, Spot 4, Int 55.08%, Obj 88.08%, Dif 73.67% TITLE 3: X 56.400, Y 529.509, Z -81.867, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00015 10200.00 10000.00 0.00 0.00042 10400.00 10000.00 0.00 0.00057 10600.00 10000.00 0.00 0.00070 10800.00 10000.00 0.00 0.00080 11000.00 10000.00 0.00 0.00100 11200.00 10000.00 0.00 0.00118 11400.00 10000.00 0.00 0.00121 11600.00 10000.00 0.00 0.00129 11800.00 10000.00 0.00 0.00144 12000.00 10000.00 0.00 0.00159 12200.00 10000.00 0.00 0.00182 12400.00 10000.00 0.00 0.00213 12600.00 10000.00 0.00 0.00234 12800.00 10000.00 0.00 0.00255 13000.00 10000.00 0.00 0.00282 13200.00 10000.00 0.00 0.00317 13400.00 10000.00 0.00 0.00365 13600.00 10000.00 0.00 0.00402 13800.00 10000.00 0.00 0.00447 14000.00 10000.00 0.00 0.00508 14200.00 10000.00 0.00 0.00561 14400.00 10000.00 0.00 0.00615 14600.00 10000.00 0.00 0.00680 14800.00 10000.00 0.00 0.00770 15000.00 10000.00 0.00 0.00870 15200.00 10000.00 0.00 0.00953 15400.00 10000.00 0.00 0.01042 15600.00 10000.00 0.00 0.01150 15800.00 10000.00 0.00 0.01266 16000.00 10000.00 0.00 0.01382 16200.00 10000.00 0.00 0.01486 16400.00 10000.00 0.00 0.01586 16600.00 10000.00 0.00 0.01700 16800.00 10000.00 0.00 0.01806 17000.00 10000.00 0.00 0.01898 17200.00 10000.00 0.00 0.02010 17400.00 10000.00 0.00 0.02132 17600.00 10000.00 0.00 0.02240 17800.00 10000.00 0.00 0.02365 18000.00 10000.00 0.00 0.02509 18200.00 10000.00 0.00 0.02644 18400.00 10000.00 0.00 0.02773 18600.00 10000.00 0.00 0.02900 18800.00 10000.00 0.00 0.03028 19000.00 10000.00 0.00 0.03147 19200.00 10000.00 0.00 0.03278 19400.00 10000.00 0.00 0.03435 19600.00 10000.00 0.00 0.03592 19800.00 10000.00 0.00 0.03736 20000.00 10000.00 0.00 0.03871 20200.00 10000.00 0.00 0.04025 20400.00 10000.00 0.00 0.04195 20600.00 10000.00 0.00 0.04367 20800.00 10000.00 0.00 0.04540 21000.00 10000.00 0.00 0.04707 21200.00 10000.00 0.00 0.04859 21400.00 10000.00 0.00 0.04976 21600.00 10000.00 0.00 0.05050 21800.00 10000.00 0.00 0.05092 22000.00 10000.00 0.00 0.05125 22200.00 10000.00 0.00 0.05141 21800.00 10200.00 0.00 0.05158 22000.00 10200.00 0.00 0.05192 22200.00 10200.00 0.00 0.05208 21800.00 10400.00 0.00 0.05220 22000.00 10400.00 0.00 0.05254 22200.00 10400.00 0.00 0.05267 21800.00 10600.00 0.00 0.05289 22000.00 10600.00 0.00 0.05324 22200.00 10600.00 0.00 0.05334 21800.00 10800.00 0.00 0.05360 22000.00 10800.00 0.00 0.05392 22200.00 10800.00 0.00 0.05398 21800.00 11000.00 0.00 0.05434 22000.00 11000.00 0.00 0.05464 22200.00 11000.00 0.00 0.05469 21800.00 11200.00 0.00 0.05511 22000.00 11200.00 0.00 0.05539 22200.00 11200.00 0.00 0.05542 21600.00 11400.00 0.00 0.05550 21800.00 11400.00 0.00 0.05598 22000.00 11400.00 0.00 0.05620 21600.00 11600.00 0.00 0.05624 21800.00 11600.00 0.00 0.05667 22000.00 11600.00 0.00 0.05685 21600.00 11800.00 0.00 0.05700 21800.00 11800.00 0.00 0.05739 22000.00 11800.00 0.00 0.05755 21600.00 12000.00 0.00 0.05778 21800.00 12000.00 0.00 0.05815 22000.00 12000.00 0.00 0.05831 22200.00 12000.00 0.00 0.05832 21600.00 12200.00 0.00 0.05847 21800.00 12200.00 0.00 0.05886 22000.00 12200.00 0.00 0.05906 22200.00 12200.00 0.00 0.05911 21600.00 12400.00 0.00 0.05930 21800.00 12400.00 0.00 0.05974 22000.00 12400.00 0.00 0.05997 22200.00 12400.00 0.00 0.06002 21600.00 12600.00 0.00 0.06027 21800.00 12600.00 0.00 0.06074 22000.00 12600.00 0.00 0.06097 22200.00 12600.00 0.00 0.06100 21600.00 12800.00 0.00 0.06141 21800.00 12800.00 0.00 0.06189 22000.00 12800.00 0.00 0.06211 22200.00 12800.00 0.00 0.06213 21600.00 13000.00 0.00 0.06246 21800.00 13000.00 0.00 0.06292 22000.00 13000.00 0.00 0.06310 21600.00 13200.00 0.00 0.06340 21800.00 13200.00 0.00 0.06380 22000.00 13200.00 0.00 0.06392 21600.00 13400.00 0.00 0.06425 21800.00 13400.00 0.00 0.06460 22000.00 13400.00 0.00 0.06469 21600.00 13600.00 0.00 0.06519 21800.00 13600.00 0.00 0.06555 22000.00 13600.00 0.00 0.06563 21600.00 13800.00 0.00 0.06627 21800.00 13800.00 0.00 0.06663 22000.00 13800.00 0.00 0.06668 21600.00 14000.00 0.00 0.06734 21800.00 14000.00 0.00 0.06766 21400.00 14200.00 0.00 0.06775 21600.00 14200.00 0.00 0.06861 21800.00 14200.00 0.00 0.06888 21400.00 14400.00 0.00 0.06911 21600.00 14400.00 0.00 0.06994 21800.00 14400.00 0.00 0.07015 21400.00 14600.00 0.00 0.07053 21600.00 14600.00 0.00 0.07131 21800.00 14600.00 0.00 0.07148 21400.00 14800.00 0.00 0.07196 21600.00 14800.00 0.00 0.07272 21800.00 14800.00 0.00 0.07288 21400.00 15000.00 0.00 0.07344 21600.00 15000.00 0.00 0.07418 21800.00 15000.00 0.00 0.07431 21400.00 15200.00 0.00 0.07480 21600.00 15200.00 0.00 0.07550 21800.00 15200.00 0.00 0.07559 21400.00 15400.00 0.00 0.07616 21600.00 15400.00 0.00 0.07682 21800.00 15400.00 0.00 0.07694 21400.00 15600.00 0.00 0.07773 21600.00 15600.00 0.00 0.07841 21800.00 15600.00 0.00 0.07854 21400.00 15800.00 0.00 0.07924 21600.00 15800.00 0.00 0.07988 21800.00 15800.00 0.00 0.07998 21400.00 16000.00 0.00 0.08074 21600.00 16000.00 0.00 0.08133 21800.00 16000.00 0.00 0.08138 21400.00 16200.00 0.00 0.08219 21600.00 16200.00 0.00 0.08276 21800.00 16200.00 0.00 0.08281 21400.00 16400.00 0.00 0.08383 21600.00 16400.00 0.00 0.08442 21800.00 16400.00 0.00 0.08445 21400.00 16600.00 0.00 0.08558 21600.00 16600.00 0.00 0.08611 21400.00 16800.00 0.00 0.08724 21600.00 16800.00 0.00 0.08769 21200.00 17000.00 0.00 0.08779 21400.00 17000.00 0.00 0.08890 21600.00 17000.00 0.00 0.08934 21200.00 17200.00 0.00 0.08941 21400.00 17200.00 0.00 0.09051 21600.00 17200.00 0.00 0.09099 21200.00 17400.00 0.00 0.09118 21400.00 17400.00 0.00 0.09228 21600.00 17400.00 0.00 0.09276 21200.00 17600.00 0.00 0.09282 21400.00 17600.00 0.00 0.09391 21600.00 17600.00 0.00 0.09438 21200.00 17800.00 0.00 0.09448 21400.00 17800.00 0.00 0.09558 21600.00 17800.00 0.00 0.09602 21200.00 18000.00 0.00 0.09626 21400.00 18000.00 0.00 0.09733 21600.00 18000.00 0.00 0.09776 21200.00 18200.00 0.00 0.09815 21400.00 18200.00 0.00 0.09921 21600.00 18200.00 0.00 0.09963 21200.00 18400.00 0.00 0.10008 21400.00 18400.00 0.00 0.10114 21600.00 18400.00 0.00 0.10157 21200.00 18600.00 0.00 0.10202 21400.00 18600.00 0.00 0.10313 21600.00 18600.00 0.00 0.10356 21200.00 18800.00 0.00 0.10423 21400.00 18800.00 0.00 0.10535 21600.00 18800.00 0.00 0.10573 21200.00 19000.00 0.00 0.10649 21400.00 19000.00 0.00 0.10757 21600.00 19000.00 0.00 0.10785 21200.00 19200.00 0.00 0.10891 21400.00 19200.00 0.00 0.10995 21600.00 19200.00 0.00 0.11016 21200.00 19400.00 0.00 0.11141 21400.00 19400.00 0.00 0.11242 21600.00 19400.00 0.00 0.11255 21200.00 19600.00 0.00 0.11406 21400.00 19600.00 0.00 0.11500 21600.00 19600.00 0.00 0.11501 21200.00 19800.00 0.00 0.11658 21400.00 19800.00 0.00 0.11743 21200.00 20000.00 0.00 0.11916 21400.00 20000.00 0.00 0.11992 21000.00 20200.00 0.00 0.12007 21200.00 20200.00 0.00 0.12204 21400.00 20200.00 0.00 0.12268 21000.00 20400.00 0.00 0.12317 21200.00 20400.00 0.00 0.12510 21400.00 20400.00 0.00 0.12560 21000.00 20600.00 0.00 0.12667 21200.00 20600.00 0.00 0.12847 21400.00 20600.00 0.00 0.12870 21000.00 20800.00 0.00 0.12979 21200.00 20800.00 0.00 0.13145 21000.00 21000.00 0.00 0.13305 21200.00 21000.00 0.00 0.13437 21000.00 21200.00 0.00 0.13518 21200.00 21200.00 0.00 0.13578 21000.00 21400.00 25.00 0.13581 21000.00 21400.00 30.00 0.13594 21000.00 21400.00 35.00 0.13601 21000.00 21400.00 40.00 0.13603 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21082.97 21315.19 38.84 0.13625 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/197.ctf NX, NY, NZ: 512 512 1 MODE : real