CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/194.mrc Output diagnostic file name Micrographs_0601/194.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/194.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2526.000 8104.000 Mean, RMS : 4495.124 220.8342 TITLE 1: emi2mrc: 194.emi Sun Jan 05 17:23:30 2014 TITLE 2: kV 300, Mag 78000x, Def 24120, Spot 4, Int 55.08%, Obj 88.08%, Dif 73.67% TITLE 3: X 48.499, Y 530.641, Z -81.867, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00799 10200.00 10000.00 0.00 -0.00789 10400.00 10000.00 0.00 -0.00781 10600.00 10000.00 0.00 -0.00769 10800.00 10000.00 0.00 -0.00741 11000.00 10000.00 0.00 -0.00715 11200.00 10000.00 0.00 -0.00691 11400.00 10000.00 0.00 -0.00669 11600.00 10000.00 0.00 -0.00645 11800.00 10000.00 0.00 -0.00611 12000.00 10000.00 0.00 -0.00573 12200.00 10000.00 0.00 -0.00524 12400.00 10000.00 0.00 -0.00470 12600.00 10000.00 0.00 -0.00417 12800.00 10000.00 0.00 -0.00362 13000.00 10000.00 0.00 -0.00306 13200.00 10000.00 0.00 -0.00247 13400.00 10000.00 0.00 -0.00186 13600.00 10000.00 0.00 -0.00105 13800.00 10000.00 0.00 -0.00030 14000.00 10000.00 0.00 0.00031 14200.00 10000.00 0.00 0.00104 14400.00 10000.00 0.00 0.00172 14600.00 10000.00 0.00 0.00238 14800.00 10000.00 0.00 0.00305 15000.00 10000.00 0.00 0.00379 15200.00 10000.00 0.00 0.00465 15400.00 10000.00 0.00 0.00553 15600.00 10000.00 0.00 0.00646 15800.00 10000.00 0.00 0.00742 16000.00 10000.00 0.00 0.00850 16200.00 10000.00 0.00 0.00964 16400.00 10000.00 0.00 0.01085 16600.00 10000.00 0.00 0.01203 16800.00 10000.00 0.00 0.01301 17000.00 10000.00 0.00 0.01399 17200.00 10000.00 0.00 0.01501 17400.00 10000.00 0.00 0.01612 17600.00 10000.00 0.00 0.01727 17800.00 10000.00 0.00 0.01847 18000.00 10000.00 0.00 0.01981 18200.00 10000.00 0.00 0.02124 18400.00 10000.00 0.00 0.02265 18600.00 10000.00 0.00 0.02395 18800.00 10000.00 0.00 0.02540 19000.00 10000.00 0.00 0.02689 19200.00 10000.00 0.00 0.02828 19400.00 10000.00 0.00 0.02992 19600.00 10000.00 0.00 0.03160 19800.00 10000.00 0.00 0.03329 20000.00 10000.00 0.00 0.03520 20200.00 10000.00 0.00 0.03720 20400.00 10000.00 0.00 0.03935 20600.00 10000.00 0.00 0.04162 20800.00 10000.00 0.00 0.04370 21000.00 10000.00 0.00 0.04565 21200.00 10000.00 0.00 0.04714 21400.00 10000.00 0.00 0.04789 21600.00 10000.00 0.00 0.04821 21800.00 10000.00 0.00 0.04831 21400.00 10200.00 0.00 0.04838 21600.00 10200.00 0.00 0.04870 21800.00 10200.00 0.00 0.04878 21400.00 10400.00 0.00 0.04898 21600.00 10400.00 0.00 0.04928 21800.00 10400.00 0.00 0.04934 21400.00 10600.00 0.00 0.04981 21600.00 10600.00 0.00 0.05010 21800.00 10600.00 0.00 0.05018 21400.00 10800.00 0.00 0.05059 21600.00 10800.00 0.00 0.05089 21800.00 10800.00 0.00 0.05098 21400.00 11000.00 0.00 0.05142 21600.00 11000.00 0.00 0.05171 21800.00 11000.00 0.00 0.05180 21400.00 11200.00 0.00 0.05226 21600.00 11200.00 0.00 0.05252 21800.00 11200.00 0.00 0.05261 21400.00 11400.00 0.00 0.05312 21600.00 11400.00 0.00 0.05337 21800.00 11400.00 0.00 0.05345 21400.00 11600.00 0.00 0.05399 21600.00 11600.00 0.00 0.05423 21800.00 11600.00 0.00 0.05430 21400.00 11800.00 0.00 0.05490 21600.00 11800.00 0.00 0.05510 21800.00 11800.00 0.00 0.05514 21200.00 12000.00 0.00 0.05518 21400.00 12000.00 0.00 0.05572 21600.00 12000.00 0.00 0.05588 21800.00 12000.00 0.00 0.05591 21200.00 12200.00 0.00 0.05595 21400.00 12200.00 0.00 0.05644 21600.00 12200.00 0.00 0.05663 21800.00 12200.00 0.00 0.05665 21200.00 12400.00 0.00 0.05681 21400.00 12400.00 0.00 0.05731 21600.00 12400.00 0.00 0.05753 21200.00 12600.00 0.00 0.05762 21400.00 12600.00 0.00 0.05814 21600.00 12600.00 0.00 0.05836 21400.00 12800.00 0.00 0.05882 21600.00 12800.00 0.00 0.05898 21400.00 13000.00 0.00 0.05938 21600.00 13000.00 0.00 0.05953 21200.00 13200.00 0.00 0.05966 21400.00 13200.00 0.00 0.06020 21600.00 13200.00 0.00 0.06039 21200.00 13400.00 0.00 0.06069 21400.00 13400.00 0.00 0.06130 21600.00 13400.00 0.00 0.06150 21200.00 13600.00 0.00 0.06183 21400.00 13600.00 0.00 0.06243 21600.00 13600.00 0.00 0.06259 21200.00 13800.00 0.00 0.06308 21400.00 13800.00 0.00 0.06364 21600.00 13800.00 0.00 0.06377 21200.00 14000.00 0.00 0.06440 21400.00 14000.00 0.00 0.06490 21600.00 14000.00 0.00 0.06503 21200.00 14200.00 0.00 0.06572 21400.00 14200.00 0.00 0.06619 21600.00 14200.00 0.00 0.06634 21200.00 14400.00 0.00 0.06705 21400.00 14400.00 0.00 0.06753 21600.00 14400.00 0.00 0.06770 21200.00 14600.00 0.00 0.06845 21400.00 14600.00 0.00 0.06893 21600.00 14600.00 0.00 0.06909 21200.00 14800.00 0.00 0.06984 21400.00 14800.00 0.00 0.07031 21600.00 14800.00 0.00 0.07045 21200.00 15000.00 0.00 0.07133 21400.00 15000.00 0.00 0.07179 21600.00 15000.00 0.00 0.07192 21200.00 15200.00 0.00 0.07285 21400.00 15200.00 0.00 0.07331 21600.00 15200.00 0.00 0.07342 21200.00 15400.00 0.00 0.07434 21400.00 15400.00 0.00 0.07478 21600.00 15400.00 0.00 0.07485 21200.00 15600.00 0.00 0.07585 21400.00 15600.00 0.00 0.07629 21600.00 15600.00 0.00 0.07636 21200.00 15800.00 0.00 0.07739 21400.00 15800.00 0.00 0.07787 21600.00 15800.00 0.00 0.07793 21200.00 16000.00 0.00 0.07904 21400.00 16000.00 0.00 0.07951 21600.00 16000.00 0.00 0.07955 21200.00 16200.00 0.00 0.08059 21400.00 16200.00 0.00 0.08105 21600.00 16200.00 0.00 0.08108 21200.00 16400.00 0.00 0.08226 21400.00 16400.00 0.00 0.08272 21600.00 16400.00 0.00 0.08275 21200.00 16600.00 0.00 0.08396 21400.00 16600.00 0.00 0.08444 21200.00 16800.00 0.00 0.08587 21400.00 16800.00 0.00 0.08633 21200.00 17000.00 0.00 0.08789 21400.00 17000.00 0.00 0.08825 21200.00 17200.00 0.00 0.08978 21400.00 17200.00 0.00 0.09005 21000.00 17400.00 0.00 0.09031 21200.00 17400.00 0.00 0.09189 21400.00 17400.00 0.00 0.09215 21000.00 17600.00 0.00 0.09237 21200.00 17600.00 0.00 0.09396 21400.00 17600.00 0.00 0.09420 21000.00 17800.00 0.00 0.09449 21200.00 17800.00 0.00 0.09610 21400.00 17800.00 0.00 0.09628 21000.00 18000.00 0.00 0.09661 21200.00 18000.00 0.00 0.09822 21400.00 18000.00 0.00 0.09829 21000.00 18200.00 0.00 0.09882 21200.00 18200.00 0.00 0.10039 21000.00 18400.00 0.00 0.10109 21200.00 18400.00 0.00 0.10255 21000.00 18600.00 0.00 0.10325 21200.00 18600.00 0.00 0.10462 21000.00 18800.00 0.00 0.10565 21200.00 18800.00 0.00 0.10699 21000.00 19000.00 0.00 0.10816 21200.00 19000.00 0.00 0.10942 21000.00 19200.00 0.00 0.11103 21200.00 19200.00 0.00 0.11219 21000.00 19400.00 0.00 0.11400 21200.00 19400.00 0.00 0.11506 21000.00 19600.00 0.00 0.11701 21200.00 19600.00 0.00 0.11793 21000.00 19800.00 0.00 0.12003 21200.00 19800.00 0.00 0.12079 21000.00 20000.00 0.00 0.12316 21200.00 20000.00 0.00 0.12376 20800.00 20200.00 0.00 0.12405 21000.00 20200.00 0.00 0.12646 21200.00 20200.00 0.00 0.12684 20800.00 20400.00 0.00 0.12744 21000.00 20400.00 0.00 0.12970 21200.00 20400.00 0.00 0.12977 20800.00 20600.00 0.00 0.13127 21000.00 20600.00 0.00 0.13320 20800.00 20800.00 0.00 0.13445 21000.00 20800.00 0.00 0.13600 20800.00 21000.00 0.00 0.13652 21000.00 21000.00 0.00 0.13746 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20896.15 21084.79 36.25 0.13795 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/194.ctf NX, NY, NZ: 512 512 1 MODE : real