CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/193.mrc Output diagnostic file name Micrographs_0601/193.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/193.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2588.000 9178.000 Mean, RMS : 4489.071 218.8920 TITLE 1: emi2mrc: 193.emi Sun Jan 05 17:22:18 2014 TITLE 2: kV 300, Mag 78000x, Def 24120, Spot 4, Int 55.08%, Obj 88.08%, Dif 73.67% TITLE 3: X 45.568, Y 531.061, Z -81.867, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00819 10600.00 10000.00 0.00 -0.00816 10800.00 10000.00 0.00 -0.00811 11000.00 10000.00 0.00 -0.00803 11200.00 10000.00 0.00 -0.00781 11400.00 10000.00 0.00 -0.00755 11600.00 10000.00 0.00 -0.00727 11800.00 10000.00 0.00 -0.00708 12600.00 10000.00 0.00 -0.00694 12800.00 10000.00 0.00 -0.00634 13000.00 10000.00 0.00 -0.00583 13200.00 10000.00 0.00 -0.00546 13400.00 10000.00 0.00 -0.00509 13600.00 10000.00 0.00 -0.00483 13800.00 10000.00 0.00 -0.00464 14000.00 10000.00 0.00 -0.00410 14200.00 10000.00 0.00 -0.00343 14400.00 10000.00 0.00 -0.00286 14600.00 10000.00 0.00 -0.00215 14800.00 10000.00 0.00 -0.00146 15000.00 10000.00 0.00 -0.00081 15200.00 10000.00 0.00 -0.00002 15400.00 10000.00 0.00 0.00079 15600.00 10000.00 0.00 0.00172 15800.00 10000.00 0.00 0.00277 16000.00 10000.00 0.00 0.00381 16200.00 10000.00 0.00 0.00485 16400.00 10000.00 0.00 0.00588 16600.00 10000.00 0.00 0.00687 16800.00 10000.00 0.00 0.00783 17000.00 10000.00 0.00 0.00888 17200.00 10000.00 0.00 0.01008 17400.00 10000.00 0.00 0.01134 17600.00 10000.00 0.00 0.01264 17800.00 10000.00 0.00 0.01397 18000.00 10000.00 0.00 0.01517 18200.00 10000.00 0.00 0.01625 18400.00 10000.00 0.00 0.01731 18600.00 10000.00 0.00 0.01852 18800.00 10000.00 0.00 0.01985 19000.00 10000.00 0.00 0.02115 19200.00 10000.00 0.00 0.02243 19400.00 10000.00 0.00 0.02366 19600.00 10000.00 0.00 0.02481 19800.00 10000.00 0.00 0.02598 20000.00 10000.00 0.00 0.02728 20200.00 10000.00 0.00 0.02879 20400.00 10000.00 0.00 0.03038 20600.00 10000.00 0.00 0.03204 20800.00 10000.00 0.00 0.03376 21000.00 10000.00 0.00 0.03565 21200.00 10000.00 0.00 0.03768 21400.00 10000.00 0.00 0.03977 21600.00 10000.00 0.00 0.04195 21800.00 10000.00 0.00 0.04385 22000.00 10000.00 0.00 0.04505 22200.00 10000.00 0.00 0.04564 22400.00 10000.00 0.00 0.04598 22600.00 10000.00 0.00 0.04606 22200.00 10200.00 0.00 0.04624 22400.00 10200.00 0.00 0.04658 22600.00 10200.00 0.00 0.04664 22200.00 10400.00 0.00 0.04671 22400.00 10400.00 0.00 0.04701 22200.00 10600.00 0.00 0.04713 22400.00 10600.00 0.00 0.04739 22200.00 10800.00 0.00 0.04768 22400.00 10800.00 0.00 0.04792 22600.00 10800.00 0.00 0.04793 22000.00 11000.00 0.00 0.04793 22200.00 11000.00 0.00 0.04854 22400.00 11000.00 0.00 0.04877 22600.00 11000.00 0.00 0.04881 22000.00 11200.00 0.00 0.04885 22200.00 11200.00 0.00 0.04941 22400.00 11200.00 0.00 0.04962 22600.00 11200.00 0.00 0.04965 22200.00 11400.00 0.00 0.05011 22400.00 11400.00 0.00 0.05030 22600.00 11400.00 0.00 0.05030 22200.00 11600.00 0.00 0.05076 22400.00 11600.00 0.00 0.05096 22000.00 11800.00 0.00 0.05111 22200.00 11800.00 0.00 0.05154 22400.00 11800.00 0.00 0.05178 22000.00 12000.00 0.00 0.05191 22200.00 12000.00 0.00 0.05236 22400.00 12000.00 0.00 0.05264 22000.00 12200.00 0.00 0.05274 22200.00 12200.00 0.00 0.05323 22400.00 12200.00 0.00 0.05353 22000.00 12400.00 0.00 0.05362 22200.00 12400.00 0.00 0.05415 22400.00 12400.00 0.00 0.05442 22200.00 12600.00 0.00 0.05496 22400.00 12600.00 0.00 0.05518 22000.00 12800.00 0.00 0.05527 22200.00 12800.00 0.00 0.05583 22400.00 12800.00 0.00 0.05602 22000.00 13000.00 0.00 0.05623 22200.00 13000.00 0.00 0.05680 22400.00 13000.00 0.00 0.05697 22000.00 13200.00 0.00 0.05720 22200.00 13200.00 0.00 0.05775 22400.00 13200.00 0.00 0.05789 22000.00 13400.00 0.00 0.05811 22200.00 13400.00 0.00 0.05863 22400.00 13400.00 0.00 0.05871 22000.00 13600.00 0.00 0.05916 22200.00 13600.00 0.00 0.05963 22400.00 13600.00 0.00 0.05968 22000.00 13800.00 0.00 0.06026 22200.00 13800.00 0.00 0.06070 22400.00 13800.00 0.00 0.06074 22000.00 14000.00 0.00 0.06137 22200.00 14000.00 0.00 0.06180 22000.00 14200.00 0.00 0.06253 22200.00 14200.00 0.00 0.06290 22000.00 14400.00 0.00 0.06343 22200.00 14400.00 0.00 0.06377 22000.00 14600.00 0.00 0.06443 22200.00 14600.00 0.00 0.06483 22400.00 14600.00 0.00 0.06485 22000.00 14800.00 0.00 0.06570 22200.00 14800.00 0.00 0.06611 22400.00 14800.00 0.00 0.06612 22000.00 15000.00 0.00 0.06702 22200.00 15000.00 0.00 0.06741 22000.00 15200.00 0.00 0.06835 22200.00 15200.00 0.00 0.06879 22400.00 15200.00 0.00 0.06880 22000.00 15400.00 0.00 0.06970 22200.00 15400.00 0.00 0.07016 22000.00 15600.00 0.00 0.07108 22200.00 15600.00 0.00 0.07152 22000.00 15800.00 0.00 0.07246 22200.00 15800.00 0.00 0.07285 22000.00 16000.00 0.00 0.07390 22200.00 16000.00 0.00 0.07425 22000.00 16200.00 0.00 0.07540 22200.00 16200.00 0.00 0.07571 22000.00 16400.00 0.00 0.07693 22200.00 16400.00 0.00 0.07724 21800.00 16600.00 0.00 0.07726 22000.00 16600.00 0.00 0.07861 22200.00 16600.00 0.00 0.07890 21800.00 16800.00 0.00 0.07913 22000.00 16800.00 0.00 0.08045 22200.00 16800.00 0.00 0.08063 21800.00 17000.00 0.00 0.08071 22000.00 17000.00 0.00 0.08198 22200.00 17000.00 0.00 0.08209 21800.00 17200.00 0.00 0.08229 22000.00 17200.00 0.00 0.08359 22200.00 17200.00 0.00 0.08368 21800.00 17400.00 0.00 0.08414 22000.00 17400.00 0.00 0.08541 22200.00 17400.00 0.00 0.08543 21800.00 17600.00 0.00 0.08577 22000.00 17600.00 0.00 0.08701 21800.00 17800.00 0.00 0.08759 22000.00 17800.00 0.00 0.08881 21800.00 18000.00 0.00 0.08951 22000.00 18000.00 0.00 0.09067 21800.00 18200.00 0.00 0.09146 22000.00 18200.00 0.00 0.09253 21800.00 18400.00 0.00 0.09347 22000.00 18400.00 0.00 0.09440 21800.00 18600.00 0.00 0.09538 22000.00 18600.00 0.00 0.09617 21800.00 18800.00 0.00 0.09717 22000.00 18800.00 0.00 0.09787 21800.00 19000.00 0.00 0.09896 22000.00 19000.00 0.00 0.09966 21800.00 19200.00 0.00 0.10101 22000.00 19200.00 0.00 0.10170 21800.00 19400.00 0.00 0.10309 22000.00 19400.00 0.00 0.10380 21800.00 19600.00 0.00 0.10555 22000.00 19600.00 0.00 0.10625 21800.00 19800.00 0.00 0.10797 22000.00 19800.00 0.00 0.10859 21800.00 20000.00 0.00 0.11030 22000.00 20000.00 0.00 0.11083 21800.00 20200.00 0.00 0.11281 22000.00 20200.00 0.00 0.11328 21600.00 20400.00 0.00 0.11336 21800.00 20400.00 0.00 0.11534 22000.00 20400.00 0.00 0.11569 21600.00 20600.00 0.00 0.11617 21800.00 20600.00 0.00 0.11807 22000.00 20600.00 0.00 0.11827 21600.00 20800.00 0.00 0.11897 21800.00 20800.00 0.00 0.12080 22000.00 20800.00 0.00 0.12081 21600.00 21000.00 0.00 0.12210 21800.00 21000.00 0.00 0.12379 21600.00 21200.00 0.00 0.12504 21800.00 21200.00 0.00 0.12651 21600.00 21400.00 0.00 0.12802 21800.00 21400.00 0.00 0.12913 21600.00 21600.00 0.00 0.13078 21800.00 21600.00 0.00 0.13117 21600.00 21800.00 0.00 0.13157 21600.00 21800.00 5.00 0.13169 21600.00 21800.00 10.00 0.13180 21600.00 21800.00 15.00 0.13191 21600.00 21800.00 20.00 0.13199 21600.00 21800.00 25.00 0.13206 21600.00 21800.00 30.00 0.13210 21600.00 21800.00 35.00 0.13211 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21608.39 21826.18 35.00 0.13214 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/193.ctf NX, NY, NZ: 512 512 1 MODE : real