CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/188.mrc Output diagnostic file name Micrographs_0601/188.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/188.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2272.000 7872.000 Mean, RMS : 4497.460 221.8291 TITLE 1: emi2mrc: 188.emi Sun Jan 05 17:18:52 2014 TITLE 2: kV 300, Mag 78000x, Def 23960, Spot 4, Int 55.08%, Obj 88.08%, Dif 73.67% TITLE 3: X 45.415, Y 533.495, Z -81.475, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01159 10200.00 10000.00 0.00 -0.01149 11400.00 10000.00 0.00 -0.01140 11600.00 10000.00 0.00 -0.01123 11800.00 10000.00 0.00 -0.01101 12000.00 10000.00 0.00 -0.01084 12200.00 10000.00 0.00 -0.01076 12400.00 10000.00 0.00 -0.01074 12600.00 10000.00 0.00 -0.01065 12800.00 10000.00 0.00 -0.01051 13000.00 10000.00 0.00 -0.01050 13200.00 10000.00 0.00 -0.01040 13400.00 10000.00 0.00 -0.01001 13600.00 10000.00 0.00 -0.00944 13800.00 10000.00 0.00 -0.00891 14000.00 10000.00 0.00 -0.00846 14200.00 10000.00 0.00 -0.00806 14400.00 10000.00 0.00 -0.00754 14600.00 10000.00 0.00 -0.00685 14800.00 10000.00 0.00 -0.00624 15000.00 10000.00 0.00 -0.00584 15200.00 10000.00 0.00 -0.00544 15400.00 10000.00 0.00 -0.00487 15600.00 10000.00 0.00 -0.00438 15800.00 10000.00 0.00 -0.00388 16000.00 10000.00 0.00 -0.00304 16200.00 10000.00 0.00 -0.00221 16400.00 10000.00 0.00 -0.00148 16600.00 10000.00 0.00 -0.00075 16800.00 10000.00 0.00 -0.00001 17000.00 10000.00 0.00 0.00088 17200.00 10000.00 0.00 0.00178 17400.00 10000.00 0.00 0.00272 17600.00 10000.00 0.00 0.00367 17800.00 10000.00 0.00 0.00465 18000.00 10000.00 0.00 0.00585 18200.00 10000.00 0.00 0.00709 18400.00 10000.00 0.00 0.00823 18600.00 10000.00 0.00 0.00945 18800.00 10000.00 0.00 0.01064 19000.00 10000.00 0.00 0.01167 19200.00 10000.00 0.00 0.01277 19400.00 10000.00 0.00 0.01386 19600.00 10000.00 0.00 0.01491 19800.00 10000.00 0.00 0.01605 20000.00 10000.00 0.00 0.01723 20200.00 10000.00 0.00 0.01856 20400.00 10000.00 0.00 0.01982 20600.00 10000.00 0.00 0.02121 20800.00 10000.00 0.00 0.02274 21000.00 10000.00 0.00 0.02419 21200.00 10000.00 0.00 0.02584 21400.00 10000.00 0.00 0.02750 21600.00 10000.00 0.00 0.02922 21800.00 10000.00 0.00 0.03114 22000.00 10000.00 0.00 0.03323 22200.00 10000.00 0.00 0.03546 22400.00 10000.00 0.00 0.03763 22600.00 10000.00 0.00 0.03947 22800.00 10000.00 0.00 0.04059 23000.00 10000.00 0.00 0.04110 23200.00 10000.00 0.00 0.04137 23400.00 10000.00 0.00 0.04143 23000.00 10200.00 0.00 0.04174 23200.00 10200.00 0.00 0.04196 23400.00 10200.00 0.00 0.04198 23000.00 10400.00 0.00 0.04225 23200.00 10400.00 0.00 0.04243 23400.00 10400.00 0.00 0.04243 23000.00 10600.00 0.00 0.04270 23200.00 10600.00 0.00 0.04286 23400.00 10600.00 0.00 0.04290 23600.00 10600.00 0.00 0.04294 23800.00 10600.00 0.00 0.04295 23000.00 10800.00 0.00 0.04324 23200.00 10800.00 0.00 0.04344 23400.00 10800.00 0.00 0.04355 23600.00 10800.00 0.00 0.04360 23000.00 11000.00 0.00 0.04391 23200.00 11000.00 0.00 0.04416 23400.00 11000.00 0.00 0.04427 23600.00 11000.00 0.00 0.04428 23000.00 11200.00 0.00 0.04459 23200.00 11200.00 0.00 0.04486 23400.00 11200.00 0.00 0.04494 23000.00 11400.00 0.00 0.04539 23200.00 11400.00 0.00 0.04565 23400.00 11400.00 0.00 0.04571 23000.00 11600.00 0.00 0.04614 23200.00 11600.00 0.00 0.04640 23400.00 11600.00 0.00 0.04643 22800.00 11800.00 0.00 0.04648 23000.00 11800.00 0.00 0.04698 23200.00 11800.00 0.00 0.04719 23400.00 11800.00 0.00 0.04719 22800.00 12000.00 0.00 0.04731 23000.00 12000.00 0.00 0.04777 23200.00 12000.00 0.00 0.04794 22800.00 12200.00 0.00 0.04801 23000.00 12200.00 0.00 0.04844 23200.00 12200.00 0.00 0.04860 23400.00 12200.00 0.00 0.04863 23600.00 12200.00 0.00 0.04863 22800.00 12400.00 0.00 0.04873 23000.00 12400.00 0.00 0.04914 23200.00 12400.00 0.00 0.04934 23400.00 12400.00 0.00 0.04939 23000.00 12600.00 0.00 0.04975 23200.00 12600.00 0.00 0.04996 23400.00 12600.00 0.00 0.05000 23000.00 12800.00 0.00 0.05045 23200.00 12800.00 0.00 0.05064 22800.00 13000.00 0.00 0.05074 23000.00 13000.00 0.00 0.05119 23200.00 13000.00 0.00 0.05133 22800.00 13200.00 0.00 0.05146 23000.00 13200.00 0.00 0.05188 23200.00 13200.00 0.00 0.05198 22800.00 13400.00 0.00 0.05219 23000.00 13400.00 0.00 0.05261 23200.00 13400.00 0.00 0.05274 23400.00 13400.00 0.00 0.05277 22800.00 13600.00 0.00 0.05308 23000.00 13600.00 0.00 0.05350 23200.00 13600.00 0.00 0.05364 23400.00 13600.00 0.00 0.05367 22800.00 13800.00 0.00 0.05397 23000.00 13800.00 0.00 0.05435 23200.00 13800.00 0.00 0.05447 22800.00 14000.00 0.00 0.05477 23000.00 14000.00 0.00 0.05513 23200.00 14000.00 0.00 0.05525 23400.00 14000.00 0.00 0.05525 22800.00 14200.00 0.00 0.05568 23000.00 14200.00 0.00 0.05607 23200.00 14200.00 0.00 0.05623 23400.00 14200.00 0.00 0.05626 22800.00 14400.00 0.00 0.05663 23000.00 14400.00 0.00 0.05707 23200.00 14400.00 0.00 0.05725 23400.00 14400.00 0.00 0.05729 22800.00 14600.00 0.00 0.05777 23000.00 14600.00 0.00 0.05821 23200.00 14600.00 0.00 0.05834 22800.00 14800.00 0.00 0.05886 23000.00 14800.00 0.00 0.05923 23200.00 14800.00 0.00 0.05932 22800.00 15000.00 0.00 0.05988 23000.00 15000.00 0.00 0.06025 23200.00 15000.00 0.00 0.06036 23400.00 15000.00 0.00 0.06038 22800.00 15200.00 0.00 0.06109 23000.00 15200.00 0.00 0.06145 23200.00 15200.00 0.00 0.06159 23400.00 15200.00 0.00 0.06162 22800.00 15400.00 0.00 0.06218 23000.00 15400.00 0.00 0.06256 23200.00 15400.00 0.00 0.06272 23400.00 15400.00 0.00 0.06272 22800.00 15600.00 0.00 0.06330 23000.00 15600.00 0.00 0.06375 23200.00 15600.00 0.00 0.06392 22800.00 15800.00 0.00 0.06466 23000.00 15800.00 0.00 0.06514 23200.00 15800.00 0.00 0.06526 22800.00 16000.00 0.00 0.06610 23000.00 16000.00 0.00 0.06654 23200.00 16000.00 0.00 0.06658 22800.00 16200.00 0.00 0.06746 23000.00 16200.00 0.00 0.06788 22800.00 16400.00 0.00 0.06879 23000.00 16400.00 0.00 0.06918 22800.00 16600.00 0.00 0.07002 23000.00 16600.00 0.00 0.07038 22800.00 16800.00 0.00 0.07144 23000.00 16800.00 0.00 0.07177 22600.00 17000.00 0.00 0.07188 22800.00 17000.00 0.00 0.07297 23000.00 17000.00 0.00 0.07326 22600.00 17200.00 0.00 0.07332 22800.00 17200.00 0.00 0.07440 23000.00 17200.00 0.00 0.07465 22600.00 17400.00 0.00 0.07498 22800.00 17400.00 0.00 0.07605 23000.00 17400.00 0.00 0.07626 22600.00 17600.00 0.00 0.07665 22800.00 17600.00 0.00 0.07769 23000.00 17600.00 0.00 0.07785 22600.00 17800.00 0.00 0.07828 22800.00 17800.00 0.00 0.07928 23000.00 17800.00 0.00 0.07941 22600.00 18000.00 0.00 0.07994 22800.00 18000.00 0.00 0.08091 23000.00 18000.00 0.00 0.08101 22600.00 18200.00 0.00 0.08153 22800.00 18200.00 0.00 0.08248 23000.00 18200.00 0.00 0.08258 22600.00 18400.00 0.00 0.08308 22800.00 18400.00 0.00 0.08406 23000.00 18400.00 0.00 0.08417 22600.00 18600.00 0.00 0.08481 22800.00 18600.00 0.00 0.08580 23000.00 18600.00 0.00 0.08589 22600.00 18800.00 0.00 0.08644 22800.00 18800.00 0.00 0.08743 23000.00 18800.00 0.00 0.08746 22600.00 19000.00 0.00 0.08799 22800.00 19000.00 0.00 0.08898 23000.00 19000.00 0.00 0.08899 22600.00 19200.00 0.00 0.08989 22800.00 19200.00 0.00 0.09089 22600.00 19400.00 0.00 0.09192 22800.00 19400.00 0.00 0.09287 22600.00 19600.00 0.00 0.09408 22800.00 19600.00 0.00 0.09492 22600.00 19800.00 0.00 0.09611 22800.00 19800.00 0.00 0.09683 22600.00 20000.00 0.00 0.09812 22800.00 20000.00 0.00 0.09876 22600.00 20200.00 0.00 0.10020 22800.00 20200.00 0.00 0.10079 22600.00 20400.00 0.00 0.10227 22800.00 20400.00 0.00 0.10281 22600.00 20600.00 0.00 0.10453 22800.00 20600.00 0.00 0.10497 22600.00 20800.00 0.00 0.10671 22800.00 20800.00 0.00 0.10702 22600.00 21000.00 0.00 0.10903 22800.00 21000.00 0.00 0.10913 22400.00 21200.00 0.00 0.10914 22600.00 21200.00 0.00 0.11118 22400.00 21400.00 0.00 0.11154 22600.00 21400.00 0.00 0.11348 22400.00 21600.00 0.00 0.11417 22600.00 21600.00 0.00 0.11598 22400.00 21800.00 0.00 0.11676 22600.00 21800.00 0.00 0.11840 22400.00 22000.00 0.00 0.11935 22600.00 22000.00 0.00 0.12076 22400.00 22200.00 0.00 0.12162 22600.00 22200.00 0.00 0.12277 22400.00 22400.00 0.00 0.12405 22600.00 22400.00 0.00 0.12464 22400.00 22600.00 0.00 0.12489 22400.00 22600.00 5.00 0.12504 22400.00 22600.00 10.00 0.12519 22400.00 22600.00 15.00 0.12533 22400.00 22600.00 20.00 0.12545 22400.00 22600.00 25.00 0.12556 22400.00 22600.00 30.00 0.12564 22400.00 22600.00 35.00 0.12569 22400.00 22600.00 40.00 0.12571 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22428.60 22608.06 40.00 0.12574 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/188.ctf NX, NY, NZ: 512 512 1 MODE : real