CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/179.mrc Output diagnostic file name Micrographs_0601/179.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/179.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2516.000 8820.000 Mean, RMS : 4487.880 216.3599 TITLE 1: emi2mrc: 179.emi Sun Jan 05 17:11:46 2014 TITLE 2: kV 300, Mag 78000x, Def 24120, Spot 4, Int 55.08%, Obj 88.08%, Dif 73.67% TITLE 3: X 56.901, Y 534.604, Z -81.475, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00381 10200.00 10000.00 0.00 -0.00372 10400.00 10000.00 0.00 -0.00363 10600.00 10000.00 0.00 -0.00342 10800.00 10000.00 0.00 -0.00326 11000.00 10000.00 0.00 -0.00318 11200.00 10000.00 0.00 -0.00298 11400.00 10000.00 0.00 -0.00270 11600.00 10000.00 0.00 -0.00234 11800.00 10000.00 0.00 -0.00196 12000.00 10000.00 0.00 -0.00165 12200.00 10000.00 0.00 -0.00137 12400.00 10000.00 0.00 -0.00113 12600.00 10000.00 0.00 -0.00087 12800.00 10000.00 0.00 -0.00042 13000.00 10000.00 0.00 0.00008 13200.00 10000.00 0.00 0.00069 13400.00 10000.00 0.00 0.00150 13600.00 10000.00 0.00 0.00226 13800.00 10000.00 0.00 0.00303 14000.00 10000.00 0.00 0.00378 14200.00 10000.00 0.00 0.00438 14400.00 10000.00 0.00 0.00509 14600.00 10000.00 0.00 0.00598 14800.00 10000.00 0.00 0.00702 15000.00 10000.00 0.00 0.00805 15200.00 10000.00 0.00 0.00895 15400.00 10000.00 0.00 0.00979 15600.00 10000.00 0.00 0.01080 15800.00 10000.00 0.00 0.01205 16000.00 10000.00 0.00 0.01317 16200.00 10000.00 0.00 0.01414 16400.00 10000.00 0.00 0.01528 16600.00 10000.00 0.00 0.01651 16800.00 10000.00 0.00 0.01761 17000.00 10000.00 0.00 0.01871 17200.00 10000.00 0.00 0.01981 17400.00 10000.00 0.00 0.02090 17600.00 10000.00 0.00 0.02203 17800.00 10000.00 0.00 0.02326 18000.00 10000.00 0.00 0.02461 18200.00 10000.00 0.00 0.02585 18400.00 10000.00 0.00 0.02712 18600.00 10000.00 0.00 0.02861 18800.00 10000.00 0.00 0.02999 19000.00 10000.00 0.00 0.03127 19200.00 10000.00 0.00 0.03286 19400.00 10000.00 0.00 0.03462 19600.00 10000.00 0.00 0.03643 19800.00 10000.00 0.00 0.03848 20000.00 10000.00 0.00 0.04070 20200.00 10000.00 0.00 0.04316 20400.00 10000.00 0.00 0.04570 20600.00 10000.00 0.00 0.04784 20800.00 10000.00 0.00 0.04911 21000.00 10000.00 0.00 0.04958 21200.00 10000.00 0.00 0.04976 21000.00 10200.00 0.00 0.05004 21200.00 10200.00 0.00 0.05023 21000.00 10400.00 0.00 0.05044 21200.00 10400.00 0.00 0.05067 21400.00 10400.00 0.00 0.05067 21000.00 10600.00 0.00 0.05104 21200.00 10600.00 0.00 0.05132 21400.00 10600.00 0.00 0.05135 21000.00 10800.00 0.00 0.05190 21200.00 10800.00 0.00 0.05216 21400.00 10800.00 0.00 0.05217 20800.00 11000.00 0.00 0.05220 21000.00 11000.00 0.00 0.05270 21200.00 11000.00 0.00 0.05293 21400.00 11000.00 0.00 0.05294 20800.00 11200.00 0.00 0.05296 21000.00 11200.00 0.00 0.05342 21200.00 11200.00 0.00 0.05367 21400.00 11200.00 0.00 0.05372 20800.00 11400.00 0.00 0.05395 21000.00 11400.00 0.00 0.05441 21200.00 11400.00 0.00 0.05470 21400.00 11400.00 0.00 0.05475 20800.00 11600.00 0.00 0.05495 21000.00 11600.00 0.00 0.05540 21200.00 11600.00 0.00 0.05571 21400.00 11600.00 0.00 0.05575 20800.00 11800.00 0.00 0.05582 21000.00 11800.00 0.00 0.05631 21200.00 11800.00 0.00 0.05666 21400.00 11800.00 0.00 0.05667 20800.00 12000.00 0.00 0.05668 21000.00 12000.00 0.00 0.05726 21200.00 12000.00 0.00 0.05764 20800.00 12200.00 0.00 0.05766 21000.00 12200.00 0.00 0.05829 21200.00 12200.00 0.00 0.05862 21000.00 12400.00 0.00 0.05923 21200.00 12400.00 0.00 0.05947 20800.00 12600.00 0.00 0.05948 21000.00 12600.00 0.00 0.06007 21200.00 12600.00 0.00 0.06029 20800.00 12800.00 0.00 0.06051 21000.00 12800.00 0.00 0.06109 21200.00 12800.00 0.00 0.06131 20800.00 13000.00 0.00 0.06157 21000.00 13000.00 0.00 0.06214 21200.00 13000.00 0.00 0.06234 20800.00 13200.00 0.00 0.06277 21000.00 13200.00 0.00 0.06332 21200.00 13200.00 0.00 0.06349 20800.00 13400.00 0.00 0.06397 21000.00 13400.00 0.00 0.06447 21200.00 13400.00 0.00 0.06464 20800.00 13600.00 0.00 0.06518 21000.00 13600.00 0.00 0.06564 21200.00 13600.00 0.00 0.06583 20800.00 13800.00 0.00 0.06643 21000.00 13800.00 0.00 0.06687 21200.00 13800.00 0.00 0.06710 20800.00 14000.00 0.00 0.06762 21000.00 14000.00 0.00 0.06811 21200.00 14000.00 0.00 0.06837 20800.00 14200.00 0.00 0.06899 21000.00 14200.00 0.00 0.06952 21200.00 14200.00 0.00 0.06975 20800.00 14400.00 0.00 0.07033 21000.00 14400.00 0.00 0.07084 21200.00 14400.00 0.00 0.07100 20800.00 14600.00 0.00 0.07177 21000.00 14600.00 0.00 0.07221 21200.00 14600.00 0.00 0.07230 20800.00 14800.00 0.00 0.07311 21000.00 14800.00 0.00 0.07351 21200.00 14800.00 0.00 0.07358 20800.00 15000.00 0.00 0.07441 21000.00 15000.00 0.00 0.07484 21200.00 15000.00 0.00 0.07493 20800.00 15200.00 0.00 0.07593 21000.00 15200.00 0.00 0.07639 21200.00 15200.00 0.00 0.07645 20800.00 15400.00 0.00 0.07758 21000.00 15400.00 0.00 0.07799 21200.00 15400.00 0.00 0.07799 20600.00 15600.00 0.00 0.07821 20800.00 15600.00 0.00 0.07943 21000.00 15600.00 0.00 0.07979 20600.00 15800.00 0.00 0.08004 20800.00 15800.00 0.00 0.08124 21000.00 15800.00 0.00 0.08159 20600.00 16000.00 0.00 0.08179 20800.00 16000.00 0.00 0.08302 21000.00 16000.00 0.00 0.08334 20600.00 16200.00 0.00 0.08360 20800.00 16200.00 0.00 0.08480 21000.00 16200.00 0.00 0.08506 20600.00 16400.00 0.00 0.08533 20800.00 16400.00 0.00 0.08647 21000.00 16400.00 0.00 0.08668 20600.00 16600.00 0.00 0.08691 20800.00 16600.00 0.00 0.08806 21000.00 16600.00 0.00 0.08829 20600.00 16800.00 0.00 0.08879 20800.00 16800.00 0.00 0.08995 21000.00 16800.00 0.00 0.09018 20600.00 17000.00 0.00 0.09069 20800.00 17000.00 0.00 0.09183 21000.00 17000.00 0.00 0.09200 20600.00 17200.00 0.00 0.09245 20800.00 17200.00 0.00 0.09358 21000.00 17200.00 0.00 0.09371 20600.00 17400.00 0.00 0.09446 20800.00 17400.00 0.00 0.09559 21000.00 17400.00 0.00 0.09568 20600.00 17600.00 0.00 0.09640 20800.00 17600.00 0.00 0.09750 21000.00 17600.00 0.00 0.09754 20600.00 17800.00 0.00 0.09854 20800.00 17800.00 0.00 0.09966 21000.00 17800.00 0.00 0.09969 20600.00 18000.00 0.00 0.10095 20800.00 18000.00 0.00 0.10207 20600.00 18200.00 0.00 0.10341 20800.00 18200.00 0.00 0.10445 20600.00 18400.00 0.00 0.10573 20800.00 18400.00 0.00 0.10667 20600.00 18600.00 0.00 0.10806 20800.00 18600.00 0.00 0.10897 20600.00 18800.00 0.00 0.11071 20800.00 18800.00 0.00 0.11159 20600.00 19000.00 0.00 0.11353 20800.00 19000.00 0.00 0.11436 20600.00 19200.00 0.00 0.11679 20800.00 19200.00 0.00 0.11756 20600.00 19400.00 0.00 0.12007 20800.00 19400.00 0.00 0.12068 20400.00 19600.00 0.00 0.12096 20600.00 19600.00 0.00 0.12337 20800.00 19600.00 0.00 0.12374 20400.00 19800.00 0.00 0.12442 20600.00 19800.00 0.00 0.12668 20800.00 19800.00 0.00 0.12673 20400.00 20000.00 0.00 0.12801 20600.00 20000.00 0.00 0.13002 20400.00 20200.00 0.00 0.13149 20600.00 20200.00 0.00 0.13319 20400.00 20400.00 0.00 0.13429 20600.00 20400.00 0.00 0.13565 20400.00 20600.00 0.00 0.13636 20600.00 20600.00 0.00 0.13696 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20483.11 20669.41 33.12 0.13753 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/179.ctf NX, NY, NZ: 512 512 1 MODE : real