CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/177.mrc Output diagnostic file name Micrographs_0601/177.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/177.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2154.000 8726.000 Mean, RMS : 4460.989 213.3000 TITLE 1: emi2mrc: 177.emi Sun Jan 05 17:09:31 2014 TITLE 2: kV 300, Mag 78000x, Def 24280, Spot 4, Int 55.08%, Obj 88.10%, Dif 73.67% TITLE 3: X 51.597, Y 535.262, Z -81.475, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04603 10200.00 10000.00 0.00 0.04847 10400.00 10000.00 0.00 0.05069 10600.00 10000.00 0.00 0.05331 10800.00 10000.00 0.00 0.05609 11000.00 10000.00 0.00 0.05915 11200.00 10000.00 0.00 0.06227 11400.00 10000.00 0.00 0.06555 11600.00 10000.00 0.00 0.06924 11800.00 10000.00 0.00 0.07290 12000.00 10000.00 0.00 0.07670 12200.00 10000.00 0.00 0.08039 12400.00 10000.00 0.00 0.08430 12600.00 10000.00 0.00 0.08836 12800.00 10000.00 0.00 0.09249 13000.00 10000.00 0.00 0.09668 13200.00 10000.00 0.00 0.10027 13400.00 10000.00 0.00 0.10251 13600.00 10000.00 0.00 0.10302 13200.00 10200.00 0.00 0.10357 13400.00 10200.00 0.00 0.10578 13600.00 10200.00 0.00 0.10618 13200.00 10400.00 0.00 0.10687 13400.00 10400.00 0.00 0.10900 13600.00 10400.00 0.00 0.10927 13200.00 10600.00 0.00 0.11013 13400.00 10600.00 0.00 0.11224 13600.00 10600.00 0.00 0.11244 13200.00 10800.00 0.00 0.11361 13400.00 10800.00 0.00 0.11573 13600.00 10800.00 0.00 0.11589 13200.00 11000.00 0.00 0.11756 13400.00 11000.00 0.00 0.11962 13200.00 11200.00 0.00 0.12129 13400.00 11200.00 0.00 0.12319 13200.00 11400.00 0.00 0.12509 13400.00 11400.00 0.00 0.12685 13200.00 11600.00 0.00 0.12854 13400.00 11600.00 0.00 0.13024 13200.00 11800.00 0.00 0.13233 13400.00 11800.00 0.00 0.13396 13200.00 12000.00 0.00 0.13641 13400.00 12000.00 0.00 0.13783 13200.00 12200.00 0.00 0.14039 13400.00 12200.00 0.00 0.14149 13200.00 12400.00 0.00 0.14454 13400.00 12400.00 0.00 0.14523 13000.00 12600.00 0.00 0.14537 13200.00 12600.00 0.00 0.14817 13400.00 12600.00 0.00 0.14842 13000.00 12800.00 0.00 0.14956 13200.00 12800.00 0.00 0.15188 13000.00 13000.00 0.00 0.15254 13200.00 13000.00 0.00 0.15439 13000.00 13200.00 0.00 0.15463 13200.00 13200.00 0.00 0.15606 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13284.03 13177.32 -72.76 0.15628 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/177.ctf NX, NY, NZ: 512 512 1 MODE : real