CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/172.mrc Output diagnostic file name Micrographs_0601/172.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/172.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2468.000 7562.000 Mean, RMS : 4405.074 216.5805 TITLE 1: emi2mrc: 172.emi Sun Jan 05 17:05:05 2014 TITLE 2: kV 300, Mag 78000x, Def 24280, Spot 4, Int 55.10%, Obj 88.10%, Dif 73.67% TITLE 3: X 59.587, Y 537.008, Z -81.475, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00450 10200.00 10000.00 0.00 -0.00420 10400.00 10000.00 0.00 -0.00380 10600.00 10000.00 0.00 -0.00342 10800.00 10000.00 0.00 -0.00307 11000.00 10000.00 0.00 -0.00281 11200.00 10000.00 0.00 -0.00256 11400.00 10000.00 0.00 -0.00217 11600.00 10000.00 0.00 -0.00170 11800.00 10000.00 0.00 -0.00118 12000.00 10000.00 0.00 -0.00072 12200.00 10000.00 0.00 -0.00017 12400.00 10000.00 0.00 0.00062 12600.00 10000.00 0.00 0.00138 12800.00 10000.00 0.00 0.00210 13000.00 10000.00 0.00 0.00291 13200.00 10000.00 0.00 0.00373 13400.00 10000.00 0.00 0.00454 13600.00 10000.00 0.00 0.00542 13800.00 10000.00 0.00 0.00623 14000.00 10000.00 0.00 0.00701 14200.00 10000.00 0.00 0.00790 14400.00 10000.00 0.00 0.00880 14600.00 10000.00 0.00 0.00974 14800.00 10000.00 0.00 0.01070 15000.00 10000.00 0.00 0.01161 15200.00 10000.00 0.00 0.01269 15400.00 10000.00 0.00 0.01380 15600.00 10000.00 0.00 0.01491 15800.00 10000.00 0.00 0.01608 16000.00 10000.00 0.00 0.01734 16200.00 10000.00 0.00 0.01876 16400.00 10000.00 0.00 0.02006 16600.00 10000.00 0.00 0.02122 16800.00 10000.00 0.00 0.02253 17000.00 10000.00 0.00 0.02398 17200.00 10000.00 0.00 0.02559 17400.00 10000.00 0.00 0.02732 17600.00 10000.00 0.00 0.02894 17800.00 10000.00 0.00 0.03045 18000.00 10000.00 0.00 0.03184 18200.00 10000.00 0.00 0.03332 18400.00 10000.00 0.00 0.03496 18600.00 10000.00 0.00 0.03656 18800.00 10000.00 0.00 0.03828 19000.00 10000.00 0.00 0.04003 19200.00 10000.00 0.00 0.04158 19400.00 10000.00 0.00 0.04304 19600.00 10000.00 0.00 0.04461 19800.00 10000.00 0.00 0.04634 20000.00 10000.00 0.00 0.04816 20200.00 10000.00 0.00 0.04988 20400.00 10000.00 0.00 0.05117 20600.00 10000.00 0.00 0.05216 20800.00 10000.00 0.00 0.05281 21000.00 10000.00 0.00 0.05319 21200.00 10000.00 0.00 0.05358 21400.00 10000.00 0.00 0.05370 20800.00 10200.00 0.00 0.05373 21000.00 10200.00 0.00 0.05410 21200.00 10200.00 0.00 0.05447 21400.00 10200.00 0.00 0.05453 20800.00 10400.00 0.00 0.05469 21000.00 10400.00 0.00 0.05502 21200.00 10400.00 0.00 0.05536 20800.00 10600.00 0.00 0.05566 21000.00 10600.00 0.00 0.05598 21200.00 10600.00 0.00 0.05629 20800.00 10800.00 0.00 0.05645 21000.00 10800.00 0.00 0.05678 21200.00 10800.00 0.00 0.05707 20800.00 11000.00 0.00 0.05722 21000.00 11000.00 0.00 0.05758 21200.00 11000.00 0.00 0.05787 20800.00 11200.00 0.00 0.05805 21000.00 11200.00 0.00 0.05843 21200.00 11200.00 0.00 0.05871 20800.00 11400.00 0.00 0.05900 21000.00 11400.00 0.00 0.05939 21200.00 11400.00 0.00 0.05962 20800.00 11600.00 0.00 0.06011 21000.00 11600.00 0.00 0.06047 21200.00 11600.00 0.00 0.06066 20600.00 11800.00 0.00 0.06079 20800.00 11800.00 0.00 0.06137 21000.00 11800.00 0.00 0.06170 21200.00 11800.00 0.00 0.06185 20600.00 12000.00 0.00 0.06194 20800.00 12000.00 0.00 0.06247 21000.00 12000.00 0.00 0.06274 21200.00 12000.00 0.00 0.06287 20800.00 12200.00 0.00 0.06336 21000.00 12200.00 0.00 0.06363 21200.00 12200.00 0.00 0.06377 20600.00 12400.00 0.00 0.06385 20800.00 12400.00 0.00 0.06439 21000.00 12400.00 0.00 0.06468 21200.00 12400.00 0.00 0.06486 20600.00 12600.00 0.00 0.06487 20800.00 12600.00 0.00 0.06544 21000.00 12600.00 0.00 0.06577 21200.00 12600.00 0.00 0.06595 20600.00 12800.00 0.00 0.06600 20800.00 12800.00 0.00 0.06661 21000.00 12800.00 0.00 0.06696 21200.00 12800.00 0.00 0.06713 20600.00 13000.00 0.00 0.06718 20800.00 13000.00 0.00 0.06781 21000.00 13000.00 0.00 0.06814 21200.00 13000.00 0.00 0.06828 20600.00 13200.00 0.00 0.06838 20800.00 13200.00 0.00 0.06900 21000.00 13200.00 0.00 0.06929 21200.00 13200.00 0.00 0.06938 20600.00 13400.00 0.00 0.06960 20800.00 13400.00 0.00 0.07019 21000.00 13400.00 0.00 0.07044 21200.00 13400.00 0.00 0.07050 20600.00 13600.00 0.00 0.07082 20800.00 13600.00 0.00 0.07135 21000.00 13600.00 0.00 0.07156 21200.00 13600.00 0.00 0.07158 20600.00 13800.00 0.00 0.07207 20800.00 13800.00 0.00 0.07256 21000.00 13800.00 0.00 0.07276 21200.00 13800.00 0.00 0.07280 20600.00 14000.00 0.00 0.07340 20800.00 14000.00 0.00 0.07389 21000.00 14000.00 0.00 0.07413 21200.00 14000.00 0.00 0.07418 20600.00 14200.00 0.00 0.07487 20800.00 14200.00 0.00 0.07536 21000.00 14200.00 0.00 0.07560 21200.00 14200.00 0.00 0.07563 20600.00 14400.00 0.00 0.07630 20800.00 14400.00 0.00 0.07680 21000.00 14400.00 0.00 0.07706 21200.00 14400.00 0.00 0.07707 20400.00 14600.00 0.00 0.07710 20600.00 14600.00 0.00 0.07796 20800.00 14600.00 0.00 0.07850 21000.00 14600.00 0.00 0.07877 20400.00 14800.00 0.00 0.07882 20600.00 14800.00 0.00 0.07971 20800.00 14800.00 0.00 0.08027 21000.00 14800.00 0.00 0.08051 20600.00 15000.00 0.00 0.08130 20800.00 15000.00 0.00 0.08185 21000.00 15000.00 0.00 0.08198 20600.00 15200.00 0.00 0.08280 20800.00 15200.00 0.00 0.08331 21000.00 15200.00 0.00 0.08337 20400.00 15400.00 0.00 0.08348 20600.00 15400.00 0.00 0.08435 20800.00 15400.00 0.00 0.08481 20400.00 15600.00 0.00 0.08507 20600.00 15600.00 0.00 0.08590 20800.00 15600.00 0.00 0.08629 20400.00 15800.00 0.00 0.08667 20600.00 15800.00 0.00 0.08748 20800.00 15800.00 0.00 0.08784 20400.00 16000.00 0.00 0.08843 20600.00 16000.00 0.00 0.08922 20800.00 16000.00 0.00 0.08954 20400.00 16200.00 0.00 0.09009 20600.00 16200.00 0.00 0.09087 20800.00 16200.00 0.00 0.09116 20400.00 16400.00 0.00 0.09199 20600.00 16400.00 0.00 0.09276 20800.00 16400.00 0.00 0.09301 20400.00 16600.00 0.00 0.09385 20600.00 16600.00 0.00 0.09457 20800.00 16600.00 0.00 0.09476 20400.00 16800.00 0.00 0.09557 20600.00 16800.00 0.00 0.09625 20800.00 16800.00 0.00 0.09639 20400.00 17000.00 0.00 0.09742 20600.00 17000.00 0.00 0.09807 20800.00 17000.00 0.00 0.09819 20400.00 17200.00 0.00 0.09914 20600.00 17200.00 0.00 0.09977 20800.00 17200.00 0.00 0.09979 20400.00 17400.00 0.00 0.10087 20600.00 17400.00 0.00 0.10141 20200.00 17600.00 0.00 0.10152 20400.00 17600.00 0.00 0.10254 20600.00 17600.00 0.00 0.10298 20200.00 17800.00 0.00 0.10337 20400.00 17800.00 0.00 0.10430 20600.00 17800.00 0.00 0.10466 20200.00 18000.00 0.00 0.10505 20400.00 18000.00 0.00 0.10593 20600.00 18000.00 0.00 0.10628 20200.00 18200.00 0.00 0.10677 20400.00 18200.00 0.00 0.10766 20600.00 18200.00 0.00 0.10802 20200.00 18400.00 0.00 0.10864 20400.00 18400.00 0.00 0.10954 20600.00 18400.00 0.00 0.10986 20200.00 18600.00 0.00 0.11058 20400.00 18600.00 0.00 0.11148 20600.00 18600.00 0.00 0.11172 20200.00 18800.00 0.00 0.11275 20400.00 18800.00 0.00 0.11358 20600.00 18800.00 0.00 0.11369 20200.00 19000.00 0.00 0.11477 20400.00 19000.00 0.00 0.11546 20200.00 19200.00 0.00 0.11685 20400.00 19200.00 0.00 0.11739 20200.00 19400.00 0.00 0.11866 20400.00 19400.00 0.00 0.11908 20000.00 19600.00 0.00 0.11917 20200.00 19600.00 0.00 0.12050 20400.00 19600.00 0.00 0.12078 20000.00 19800.00 0.00 0.12092 20200.00 19800.00 0.00 0.12217 20400.00 19800.00 0.00 0.12220 20000.00 20000.00 0.00 0.12230 20200.00 20000.00 0.00 0.12343 20200.00 20200.00 0.00 0.12413 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20196.69 20212.39 45.01 0.12413 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/172.ctf NX, NY, NZ: 512 512 1 MODE : real