CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/169.mrc Output diagnostic file name Micrographs_0601/169.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/169.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2176.000 8322.000 Mean, RMS : 4550.658 228.0594 TITLE 1: emi2mrc: 169.emi Sun Jan 05 16:58:22 2014 TITLE 2: kV 300, Mag 78000x, Def 24120, Spot 4, Int 55.10%, Obj 88.01%, Dif 73.67% TITLE 3: X 18.074, Y 314.261, Z -97.762, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00842 10200.00 10000.00 0.00 -0.00840 10400.00 10000.00 0.00 -0.00815 10600.00 10000.00 0.00 -0.00799 10800.00 10000.00 0.00 -0.00782 11000.00 10000.00 0.00 -0.00765 11200.00 10000.00 0.00 -0.00748 11400.00 10000.00 0.00 -0.00736 11600.00 10000.00 0.00 -0.00727 11800.00 10000.00 0.00 -0.00708 12000.00 10000.00 0.00 -0.00677 12200.00 10000.00 0.00 -0.00654 12400.00 10000.00 0.00 -0.00645 12600.00 10000.00 0.00 -0.00618 12800.00 10000.00 0.00 -0.00576 13000.00 10000.00 0.00 -0.00538 13200.00 10000.00 0.00 -0.00499 13400.00 10000.00 0.00 -0.00460 13600.00 10000.00 0.00 -0.00429 13800.00 10000.00 0.00 -0.00402 14000.00 10000.00 0.00 -0.00355 14200.00 10000.00 0.00 -0.00299 14400.00 10000.00 0.00 -0.00237 14600.00 10000.00 0.00 -0.00171 14800.00 10000.00 0.00 -0.00105 15000.00 10000.00 0.00 -0.00026 15200.00 10000.00 0.00 0.00041 15400.00 10000.00 0.00 0.00108 15600.00 10000.00 0.00 0.00193 15800.00 10000.00 0.00 0.00277 16000.00 10000.00 0.00 0.00371 16200.00 10000.00 0.00 0.00471 16400.00 10000.00 0.00 0.00580 16600.00 10000.00 0.00 0.00695 16800.00 10000.00 0.00 0.00803 17000.00 10000.00 0.00 0.00908 17200.00 10000.00 0.00 0.01010 17400.00 10000.00 0.00 0.01130 17600.00 10000.00 0.00 0.01266 17800.00 10000.00 0.00 0.01411 18000.00 10000.00 0.00 0.01568 18200.00 10000.00 0.00 0.01715 18400.00 10000.00 0.00 0.01847 18600.00 10000.00 0.00 0.01972 18800.00 10000.00 0.00 0.02106 19000.00 10000.00 0.00 0.02247 19200.00 10000.00 0.00 0.02392 19400.00 10000.00 0.00 0.02549 19600.00 10000.00 0.00 0.02719 19800.00 10000.00 0.00 0.02899 20000.00 10000.00 0.00 0.03080 20200.00 10000.00 0.00 0.03277 20400.00 10000.00 0.00 0.03489 20600.00 10000.00 0.00 0.03698 20800.00 10000.00 0.00 0.03901 21000.00 10000.00 0.00 0.04093 21200.00 10000.00 0.00 0.04262 21400.00 10000.00 0.00 0.04433 21600.00 10000.00 0.00 0.04596 21800.00 10000.00 0.00 0.04687 22000.00 10000.00 0.00 0.04731 22200.00 10000.00 0.00 0.04747 22000.00 10200.00 0.00 0.04778 22200.00 10200.00 0.00 0.04789 22000.00 10400.00 0.00 0.04818 22200.00 10400.00 0.00 0.04824 22000.00 10600.00 0.00 0.04859 22200.00 10600.00 0.00 0.04860 22000.00 10800.00 0.00 0.04889 21800.00 11000.00 0.00 0.04901 22000.00 11000.00 0.00 0.04932 22200.00 11000.00 0.00 0.04933 21800.00 11200.00 0.00 0.04952 22000.00 11200.00 0.00 0.04985 22200.00 11200.00 0.00 0.04993 21800.00 11400.00 0.00 0.05019 22000.00 11400.00 0.00 0.05058 22200.00 11400.00 0.00 0.05072 21800.00 11600.00 0.00 0.05101 22000.00 11600.00 0.00 0.05146 22200.00 11600.00 0.00 0.05161 21800.00 11800.00 0.00 0.05187 22000.00 11800.00 0.00 0.05232 22200.00 11800.00 0.00 0.05243 21800.00 12000.00 0.00 0.05276 22000.00 12000.00 0.00 0.05319 22200.00 12000.00 0.00 0.05326 21800.00 12200.00 0.00 0.05377 22000.00 12200.00 0.00 0.05415 22200.00 12200.00 0.00 0.05417 21800.00 12400.00 0.00 0.05468 22000.00 12400.00 0.00 0.05501 21800.00 12600.00 0.00 0.05547 22000.00 12600.00 0.00 0.05578 21800.00 12800.00 0.00 0.05639 22000.00 12800.00 0.00 0.05664 21800.00 13000.00 0.00 0.05728 22000.00 13000.00 0.00 0.05743 21600.00 13200.00 0.00 0.05754 21800.00 13200.00 0.00 0.05821 22000.00 13200.00 0.00 0.05832 21600.00 13400.00 0.00 0.05852 21800.00 13400.00 0.00 0.05912 22000.00 13400.00 0.00 0.05927 21600.00 13600.00 0.00 0.05953 21800.00 13600.00 0.00 0.06013 22000.00 13600.00 0.00 0.06035 22200.00 13600.00 0.00 0.06037 21600.00 13800.00 0.00 0.06077 21800.00 13800.00 0.00 0.06140 22000.00 13800.00 0.00 0.06167 22200.00 13800.00 0.00 0.06169 21600.00 14000.00 0.00 0.06213 21800.00 14000.00 0.00 0.06276 22000.00 14000.00 0.00 0.06305 22200.00 14000.00 0.00 0.06306 21600.00 14200.00 0.00 0.06345 21800.00 14200.00 0.00 0.06409 22000.00 14200.00 0.00 0.06440 22200.00 14200.00 0.00 0.06441 21600.00 14400.00 0.00 0.06476 21800.00 14400.00 0.00 0.06545 22000.00 14400.00 0.00 0.06579 21600.00 14600.00 0.00 0.06628 21800.00 14600.00 0.00 0.06699 22000.00 14600.00 0.00 0.06732 21600.00 14800.00 0.00 0.06778 21800.00 14800.00 0.00 0.06847 22000.00 14800.00 0.00 0.06875 21600.00 15000.00 0.00 0.06929 21800.00 15000.00 0.00 0.06997 22000.00 15000.00 0.00 0.07026 21600.00 15200.00 0.00 0.07092 21800.00 15200.00 0.00 0.07159 22000.00 15200.00 0.00 0.07186 21600.00 15400.00 0.00 0.07250 21800.00 15400.00 0.00 0.07313 22000.00 15400.00 0.00 0.07332 21600.00 15600.00 0.00 0.07391 21800.00 15600.00 0.00 0.07450 22000.00 15600.00 0.00 0.07464 21600.00 15800.00 0.00 0.07537 21800.00 15800.00 0.00 0.07599 22000.00 15800.00 0.00 0.07613 21600.00 16000.00 0.00 0.07715 21800.00 16000.00 0.00 0.07776 22000.00 16000.00 0.00 0.07786 21600.00 16200.00 0.00 0.07889 21800.00 16200.00 0.00 0.07946 22000.00 16200.00 0.00 0.07950 21600.00 16400.00 0.00 0.08066 21800.00 16400.00 0.00 0.08121 22000.00 16400.00 0.00 0.08124 21600.00 16600.00 0.00 0.08243 21800.00 16600.00 0.00 0.08298 22000.00 16600.00 0.00 0.08301 21600.00 16800.00 0.00 0.08435 21800.00 16800.00 0.00 0.08490 22000.00 16800.00 0.00 0.08491 21400.00 17000.00 0.00 0.08495 21600.00 17000.00 0.00 0.08632 21800.00 17000.00 0.00 0.08687 21600.00 17200.00 0.00 0.08817 21800.00 17200.00 0.00 0.08874 21400.00 17400.00 0.00 0.08878 21600.00 17400.00 0.00 0.09023 21800.00 17400.00 0.00 0.09080 21400.00 17600.00 0.00 0.09080 21600.00 17600.00 0.00 0.09228 21800.00 17600.00 0.00 0.09283 21400.00 17800.00 0.00 0.09302 21600.00 17800.00 0.00 0.09451 21800.00 17800.00 0.00 0.09501 21400.00 18000.00 0.00 0.09526 21600.00 18000.00 0.00 0.09676 21800.00 18000.00 0.00 0.09720 21400.00 18200.00 0.00 0.09758 21600.00 18200.00 0.00 0.09906 21800.00 18200.00 0.00 0.09942 21400.00 18400.00 0.00 0.10002 21600.00 18400.00 0.00 0.10143 21800.00 18400.00 0.00 0.10169 21400.00 18600.00 0.00 0.10247 21600.00 18600.00 0.00 0.10379 21800.00 18600.00 0.00 0.10396 21400.00 18800.00 0.00 0.10502 21600.00 18800.00 0.00 0.10625 21800.00 18800.00 0.00 0.10638 21400.00 19000.00 0.00 0.10750 21600.00 19000.00 0.00 0.10868 21800.00 19000.00 0.00 0.10878 21400.00 19200.00 0.00 0.11016 21600.00 19200.00 0.00 0.11133 21800.00 19200.00 0.00 0.11141 21400.00 19400.00 0.00 0.11289 21600.00 19400.00 0.00 0.11405 21400.00 19600.00 0.00 0.11576 21600.00 19600.00 0.00 0.11684 21400.00 19800.00 0.00 0.11858 21600.00 19800.00 0.00 0.11956 21200.00 20000.00 0.00 0.11963 21400.00 20000.00 0.00 0.12153 21600.00 20000.00 0.00 0.12239 21200.00 20200.00 0.00 0.12285 21400.00 20200.00 0.00 0.12461 21600.00 20200.00 0.00 0.12528 21200.00 20400.00 0.00 0.12586 21400.00 20400.00 0.00 0.12745 21600.00 20400.00 0.00 0.12790 21200.00 20600.00 0.00 0.12916 21400.00 20600.00 0.00 0.13052 21600.00 20600.00 0.00 0.13073 21200.00 20800.00 0.00 0.13228 21400.00 20800.00 0.00 0.13338 21200.00 21000.00 0.00 0.13529 21400.00 21000.00 0.00 0.13594 21200.00 21200.00 0.00 0.13723 21400.00 21200.00 0.00 0.13736 21200.00 21400.00 0.00 0.13753 21200.00 21400.00 5.00 0.13768 21200.00 21400.00 10.00 0.13783 21200.00 21400.00 15.00 0.13797 21200.00 21400.00 20.00 0.13809 21200.00 21400.00 25.00 0.13819 21200.00 21400.00 30.00 0.13827 21200.00 21400.00 35.00 0.13832 21200.00 21400.00 40.00 0.13835 21200.00 21400.00 45.00 0.13835 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21129.63 21492.77 42.14 0.13860 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/169.ctf NX, NY, NZ: 512 512 1 MODE : real