CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/162.mrc Output diagnostic file name Micrographs_0601/162.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/162.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2392.000 7812.000 Mean, RMS : 4535.720 221.2069 TITLE 1: emi2mrc: 162.emi Sun Jan 05 16:53:51 2014 TITLE 2: kV 300, Mag 78000x, Def 23790, Spot 4, Int 55.10%, Obj 88.02%, Dif 73.67% TITLE 3: X 16.271, Y 317.171, Z -97.762, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00180 10200.00 10000.00 0.00 0.00217 10400.00 10000.00 0.00 0.00262 10600.00 10000.00 0.00 0.00325 10800.00 10000.00 0.00 0.00388 11000.00 10000.00 0.00 0.00439 11200.00 10000.00 0.00 0.00485 11400.00 10000.00 0.00 0.00516 11600.00 10000.00 0.00 0.00542 11800.00 10000.00 0.00 0.00595 12000.00 10000.00 0.00 0.00659 12200.00 10000.00 0.00 0.00715 12400.00 10000.00 0.00 0.00784 12600.00 10000.00 0.00 0.00867 12800.00 10000.00 0.00 0.00950 13000.00 10000.00 0.00 0.01034 13200.00 10000.00 0.00 0.01130 13400.00 10000.00 0.00 0.01225 13600.00 10000.00 0.00 0.01314 13800.00 10000.00 0.00 0.01414 14000.00 10000.00 0.00 0.01521 14200.00 10000.00 0.00 0.01631 14400.00 10000.00 0.00 0.01739 14600.00 10000.00 0.00 0.01861 14800.00 10000.00 0.00 0.02000 15000.00 10000.00 0.00 0.02139 15200.00 10000.00 0.00 0.02281 15400.00 10000.00 0.00 0.02433 15600.00 10000.00 0.00 0.02588 15800.00 10000.00 0.00 0.02739 16000.00 10000.00 0.00 0.02898 16200.00 10000.00 0.00 0.03054 16400.00 10000.00 0.00 0.03217 16600.00 10000.00 0.00 0.03388 16800.00 10000.00 0.00 0.03543 17000.00 10000.00 0.00 0.03704 17200.00 10000.00 0.00 0.03868 17400.00 10000.00 0.00 0.04033 17600.00 10000.00 0.00 0.04213 17800.00 10000.00 0.00 0.04410 18000.00 10000.00 0.00 0.04606 18200.00 10000.00 0.00 0.04797 18400.00 10000.00 0.00 0.04999 18600.00 10000.00 0.00 0.05175 18800.00 10000.00 0.00 0.05316 19000.00 10000.00 0.00 0.05415 19200.00 10000.00 0.00 0.05475 19400.00 10000.00 0.00 0.05523 19600.00 10000.00 0.00 0.05562 19800.00 10000.00 0.00 0.05592 20000.00 10000.00 0.00 0.05607 20200.00 10000.00 0.00 0.05618 20400.00 10000.00 0.00 0.05628 20600.00 10000.00 0.00 0.05629 19600.00 10200.00 0.00 0.05653 19800.00 10200.00 0.00 0.05684 20000.00 10200.00 0.00 0.05701 20200.00 10200.00 0.00 0.05711 20400.00 10200.00 0.00 0.05719 19600.00 10400.00 0.00 0.05745 19800.00 10400.00 0.00 0.05776 20000.00 10400.00 0.00 0.05792 20200.00 10400.00 0.00 0.05798 20400.00 10400.00 0.00 0.05799 19400.00 10600.00 0.00 0.05809 19600.00 10600.00 0.00 0.05848 19800.00 10600.00 0.00 0.05877 20000.00 10600.00 0.00 0.05889 19400.00 10800.00 0.00 0.05912 19600.00 10800.00 0.00 0.05947 19800.00 10800.00 0.00 0.05973 20000.00 10800.00 0.00 0.05979 19400.00 11000.00 0.00 0.06017 19600.00 11000.00 0.00 0.06050 19800.00 11000.00 0.00 0.06073 20000.00 11000.00 0.00 0.06073 19200.00 11200.00 0.00 0.06080 19400.00 11200.00 0.00 0.06129 19600.00 11200.00 0.00 0.06159 19800.00 11200.00 0.00 0.06177 19200.00 11400.00 0.00 0.06207 19400.00 11400.00 0.00 0.06255 19600.00 11400.00 0.00 0.06280 19800.00 11400.00 0.00 0.06292 19200.00 11600.00 0.00 0.06327 19400.00 11600.00 0.00 0.06373 19600.00 11600.00 0.00 0.06395 19800.00 11600.00 0.00 0.06405 19200.00 11800.00 0.00 0.06463 19400.00 11800.00 0.00 0.06509 19600.00 11800.00 0.00 0.06530 19800.00 11800.00 0.00 0.06539 19000.00 12000.00 0.00 0.06557 19200.00 12000.00 0.00 0.06617 19400.00 12000.00 0.00 0.06662 19600.00 12000.00 0.00 0.06680 19800.00 12000.00 0.00 0.06683 19000.00 12200.00 0.00 0.06703 19200.00 12200.00 0.00 0.06762 19400.00 12200.00 0.00 0.06802 19600.00 12200.00 0.00 0.06812 19000.00 12400.00 0.00 0.06849 19200.00 12400.00 0.00 0.06902 19400.00 12400.00 0.00 0.06933 19600.00 12400.00 0.00 0.06934 19000.00 12600.00 0.00 0.06998 19200.00 12600.00 0.00 0.07045 19400.00 12600.00 0.00 0.07068 19000.00 12800.00 0.00 0.07153 19200.00 12800.00 0.00 0.07194 19400.00 12800.00 0.00 0.07214 18800.00 13000.00 0.00 0.07220 19000.00 13000.00 0.00 0.07304 19200.00 13000.00 0.00 0.07343 19400.00 13000.00 0.00 0.07364 18800.00 13200.00 0.00 0.07392 19000.00 13200.00 0.00 0.07474 19200.00 13200.00 0.00 0.07512 19400.00 13200.00 0.00 0.07535 18800.00 13400.00 0.00 0.07566 19000.00 13400.00 0.00 0.07646 19200.00 13400.00 0.00 0.07685 19400.00 13400.00 0.00 0.07709 18800.00 13600.00 0.00 0.07748 19000.00 13600.00 0.00 0.07831 19200.00 13600.00 0.00 0.07872 19400.00 13600.00 0.00 0.07897 18800.00 13800.00 0.00 0.07942 19000.00 13800.00 0.00 0.08029 19200.00 13800.00 0.00 0.08072 19400.00 13800.00 0.00 0.08094 18800.00 14000.00 0.00 0.08147 19000.00 14000.00 0.00 0.08236 19200.00 14000.00 0.00 0.08275 19400.00 14000.00 0.00 0.08292 18800.00 14200.00 0.00 0.08362 19000.00 14200.00 0.00 0.08449 19200.00 14200.00 0.00 0.08484 19400.00 14200.00 0.00 0.08496 18800.00 14400.00 0.00 0.08574 19000.00 14400.00 0.00 0.08659 19200.00 14400.00 0.00 0.08690 19400.00 14400.00 0.00 0.08698 18800.00 14600.00 0.00 0.08824 19000.00 14600.00 0.00 0.08902 19200.00 14600.00 0.00 0.08925 18800.00 14800.00 0.00 0.09062 19000.00 14800.00 0.00 0.09130 19200.00 14800.00 0.00 0.09141 18800.00 15000.00 0.00 0.09297 19000.00 15000.00 0.00 0.09353 19200.00 15000.00 0.00 0.09358 18600.00 15200.00 0.00 0.09381 18800.00 15200.00 0.00 0.09527 19000.00 15200.00 0.00 0.09574 19200.00 15200.00 0.00 0.09575 18600.00 15400.00 0.00 0.09617 18800.00 15400.00 0.00 0.09753 19000.00 15400.00 0.00 0.09793 19200.00 15400.00 0.00 0.09794 18600.00 15600.00 0.00 0.09866 18800.00 15600.00 0.00 0.09995 19000.00 15600.00 0.00 0.10036 19200.00 15600.00 0.00 0.10037 18600.00 15800.00 0.00 0.10100 18800.00 15800.00 0.00 0.10228 19000.00 15800.00 0.00 0.10270 18600.00 16000.00 0.00 0.10353 18800.00 16000.00 0.00 0.10480 19000.00 16000.00 0.00 0.10522 18600.00 16200.00 0.00 0.10609 18800.00 16200.00 0.00 0.10737 19000.00 16200.00 0.00 0.10778 18600.00 16400.00 0.00 0.10893 18800.00 16400.00 0.00 0.11021 19000.00 16400.00 0.00 0.11059 18600.00 16600.00 0.00 0.11177 18800.00 16600.00 0.00 0.11303 19000.00 16600.00 0.00 0.11330 18600.00 16800.00 0.00 0.11462 18800.00 16800.00 0.00 0.11584 19000.00 16800.00 0.00 0.11599 18600.00 17000.00 0.00 0.11771 18800.00 17000.00 0.00 0.11886 19000.00 17000.00 0.00 0.11888 18600.00 17200.00 0.00 0.12068 18800.00 17200.00 0.00 0.12174 18400.00 17400.00 0.00 0.12196 18600.00 17400.00 0.00 0.12374 18800.00 17400.00 0.00 0.12468 18400.00 17600.00 0.00 0.12503 18600.00 17600.00 0.00 0.12675 18800.00 17600.00 0.00 0.12757 18400.00 17800.00 0.00 0.12847 18600.00 17800.00 0.00 0.13005 18800.00 17800.00 0.00 0.13069 18400.00 18000.00 0.00 0.13160 18600.00 18000.00 0.00 0.13301 18800.00 18000.00 0.00 0.13345 18400.00 18200.00 0.00 0.13477 18600.00 18200.00 0.00 0.13587 18800.00 18200.00 0.00 0.13599 18400.00 18400.00 0.00 0.13728 18600.00 18400.00 0.00 0.13775 18400.00 18600.00 15.00 0.13781 18400.00 18600.00 20.00 0.13789 18400.00 18600.00 25.00 0.13798 18400.00 18600.00 30.00 0.13805 18400.00 18800.00 30.00 0.13813 18400.00 18800.00 35.00 0.13828 18400.00 18800.00 40.00 0.13840 18400.00 18800.00 45.00 0.13848 18400.00 18800.00 50.00 0.13851 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18405.97 18765.16 52.02 0.13853 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/162.ctf NX, NY, NZ: 512 512 1 MODE : real